DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RpI135 and polr1b

DIOPT Version :9

Sequence 1:	NP_476708.1	Gene:	RpI135 / 33210	FlyBaseID:	FBgn0003278	Length:	1129	Species:	Drosophila melanogaster
Sequence 2:	NP_956812.2	Gene:	polr1b / 325603	ZFINID:	ZDB-GENE-040426-1598	Length:	1132	Species:	Danio rerio

Alignment Length:	1159	Identity:	519/1159 - (44%)
Similarity:	723/1159 - (62%)	Gaps:	64/1159 - (5%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 LEEMQQMKTIPVLTNSRPEFKQIPKKLSR-HLANLGGPHVDSFDEMLTVGLDNSAKHMIPNHWLS 65
            |.:...:||.|.|.:.......:||:..| .:..|...|::|||:.:|.||....:.:.|..:|.
Zfish     3 LSKWCNLKTEPSLKHLTEAGFGVPKEKQRVPVQELVKAHIESFDQAVTDGLCRVVEAIPPMEFLY 67

  Fly    66 PAGEKISMK-VESIWIAKPKVPQDVIDVRTREIYPTDSRQLHVSYSGMCSVRLGWSVNGVQKTPI 129
             .|::||:. ||:| |..|.|.:..|. |...::|.:.|....:|.......:.||||||.|..|
Zfish    68 -KGDRISLSFVEAI-IHTPSVSKGGIS-RDLRVFPAECRGRRCTYRAKLVADVSWSVNGVPKGII 129

  Fly   130 NMDLGEVPIMLRSKACNLGQATPEEMVKHGEHDSEWGGIFVIRGNEKIVRMLIMTRRNHPICVKR 194
            ...||::|||::||.|||...:|:|:::|.|...|.||.|::.|.||::|:|||.|||:||.|.|
Zfish   130 KQSLGQMPIMVKSKLCNLHGLSPKELLEHHEEAEEMGGYFIVNGIEKVIRLLIMPRRNYPIAVSR 194

  Fly   195 SSWKDRGQNFSDLGMLVQTVREDESSLSNVVHYLNNGTAKFMFSHVKRLSYVPVCLILKCLMDYT 259
            ..:|.|||.::...:.::.|:|:.::::..:|||:|||....|.:.|.|.::|:...||.|:||:
Zfish   195 PKFKSRGQGYTQYAISMRCVKEEHTAINMNLHYLDNGTVMVNFIYRKELFFLPLGFALKALVDYS 259

  Fly   260 DEEIYNRLVQGYESDQYYVSCVQAMLREVQNENVYTHAQCKSFIGNLFRARFPEVPEWQPDDDVT 324
            |.:||..|::|.|.:.:|.|||..|||.|.::...|..:..:::|..||.:. .:|||...:...
Zfish   260 DYQIYQELIKGREDNSFYKSCVSEMLRIVMDQGCTTKIKVLNYLGERFRVKL-NLPEWFTHEQCA 323

  Fly   325 DFILRERVMIHLDTYEDKFQLIVFMIQKLFQCAQGKYKVENVDSAMMQEVLLPGHLYQKYLSERV 389
            .|:|.|.:.|||.|..:||.::..|.:|||..|:.:...||.||.|..|||.||.||..:|.|::
Zfish   324 HFLLDECLCIHLKTDVEKFYMLCLMTRKLFSFAKQECMEENPDSPMFHEVLTPGQLYLMFLKEKM 388

  Fly   390 ESWVSQVRRCLQKK------LTSPDALVTSAVMTQCMRQAGGVGRAIESFLATGNIASRTGLGLM 448
            |.|:..|:..|:||      || ||:      ||:.......:.:|.|..|||||:.|:||||::
Zfish   389 EGWLVSVKLALEKKPQRVGGLT-PDS------MTKLFNMGTDLTKAFEYLLATGNLMSKTGLGML 446

  Fly   449 QNSGLVIMAENINRMRYMSHFRAIHRGSYFTTMRTTEARQLLPDAWGFICPVHTPDGTPCGLLNH 513
            |||||.::|:.:|.:||:||||.:|||:.|..||||..|:|||::|||:||||||||.||||:||
Zfish   447 QNSGLCVVADKLNFIRYLSHFRCVHRGAAFAKMRTTTVRKLLPESWGFLCPVHTPDGEPCGLMNH 511

  Fly   514 LTLTCEISMRPDPKLVKAIPKHLIDMGMMPL-SNRRYLGEKLYVVFLDGKHLGHIHQSEAEKIVD 577
            :|..|||.....|  ...:|..|..:|:... .|........|.|.|||..:|.:.:..|..:::
Zfish   512 MTAHCEIVAPVYP--TSTLPGLLCSLGVNAADGNPGQAYSDCYPVILDGAMIGWVEKEVAPSVIE 574

  Fly   578 ELRYGKIFG--TLPQMMEIGFIP-FKKNGQFPGLYIATGPARMMRPVWNLKWKRVEYIGTLEQLY 639
            .||..|:.|  .:|...||..:| ..|...:||||:.|.|.||:|.|.||...:.|.||..||:|
Zfish   575 SLRRFKVLGEKKVPPWTEIVLVPQTGKASLYPGLYLFTTPCRMVRTVRNLSLGKQELIGIFEQVY 639

  Fly   640 MEIAIDAKEMYPDFTTHLELAKTHFMSNLANLIPMPDYNQSPRNMYQCQMGKQTMGTPCLNWPKQ 704
            :.:.|..||:....|||.||.....:|.:|..||..|:||||||||||||.|||||.|..::..:
Zfish   640 LNVGIFEKEIEDGVTTHQELFPHSMLSVVAEFIPFSDHNQSPRNMYQCQMSKQTMGFPLHSYQYR 704

  Fly   705 AANKLYRLQTPGTPLFRPVHYDNIQLDDFAMGTNAIVAVISYTGYDMEDAMIINKAAYERGFAYG 769
            :.|||||||||.:||.||..||:..:|::.:|||||||||||||||||||||:||:::|||||:|
Zfish   705 SDNKLYRLQTPQSPLVRPAMYDHYDVDNYPIGTNAIVAVISYTGYDMEDAMIVNKSSWERGFAHG 769

  Fly   770 SIYKTKFLTLDKK-----SSYFARHPHMPELIKHLDTDGLPHPGSKLSYGSPLYCYFDGEVATYK 829
            |:|||:.:.|.:|     |..|...|..|:::..||.||||..||.|.||.|.|.|.........
Zfish   770 SVYKTEIIDLSEKVKGDDSIVFGVKPGDPKVMGKLDADGLPFIGSVLKYGDPFYGYISLNTGQSH 834

  Fly   830 VVKMDEKEDCIVESIR------QLGSFDLSPKKMVAITLRVPRPATIGDKFASRAGQKGICSQKY 888
            |.....:|.|:|::|:      ..|.|     |.:.:|.||||..|||||||||.|||||.|:.:
Zfish   835 VYFYKYQESCVVDNIKVCSNETGTGRF-----KRICVTSRVPRNPTIGDKFASRHGQKGILSRLW 894

  Fly   889 PAEDLPFTESGLIPDIVFNPHGFPSRMTIAMMIETMAGKGAAIHGNVYDATPFRFSEENTAIDYF 953
            ||||:||||||:.|||:||||||||||||.|:||:||||.|::||..:|||||.|||||:|:::|
Zfish   895 PAEDMPFTESGMSPDILFNPHGFPSRMTIGMLIESMAGKSASLHGLCHDATPFTFSEENSALEHF 959

  Fly   954 GKMLEAGGYNYYGTERLYSGVDGREMTADIFFGVVHYQRLRHMVFDKWQVRSTGAVEARTHQPIK 1018
            |::|:|.|||||||||||||:.|.|:.||||.|||:||||||||.||:|||:|||.:..|:||:.
Zfish   960 GELLKAAGYNYYGTERLYSGLSGLELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDKVTNQPMG 1024

  Fly  1019 GRKRGGGVRFGEMERDALISHGAAFLLQDRLFHNSDKTHTLVCHKCGSILAPLQRIVKRNETGGL 1083
            ||...||:||||||||||::||.:|||.||||:.||::...||..|||:|:.|           |
Zfish  1025 GRNVQGGIRFGEMERDALLAHGTSFLLHDRLFNCSDRSVAQVCMDCGSLLSTL-----------L 1078

  Fly  1084 SSQPDT----------CRLCGDNSSVSMIEIPFSFKYLVTELSSVNINARFKLN 1127
            ...|..          |.||..:.|:..:.:|:.|||.|.||:::||  :.||:
Zfish  1079 EKPPPNWSATRHRKTICLLCNKSDSIETVSVPYVFKYFVAELAAMNI--KIKLD 1130

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RpI135	NP_476708.1	RpoB	34..1128	CDD:223163	511/1126 (45%)
		RNA_pol_B_RPB2	35..1125	CDD:238353	509/1121 (45%)
		RNA_pol_Rpa2_4	564..614	CDD:284335	16/52 (31%)
polr1b	NP_956812.2	RpoB	16..1132	CDD:223163	514/1146 (45%)
		RNA_pol_B_RPB2	37..1130	CDD:238353	510/1123 (45%)
		RNA_pol_Rpa2_4	561..614	CDD:284335	16/52 (31%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170580577
Domainoid	1	1.000	423	1.000	Domainoid score	I601
eggNOG	1	0.900	-	-		E1_COG0085
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H7133
Inparanoid	1	1.050	964	1.000	Inparanoid score	I310
OMA	1	1.010	-	-		QHG53969
OrthoDB	1	1.010	-	-		D42570at2759
OrthoFinder	1	1.000	-	-		FOG0000346
OrthoInspector	1	1.000	-	-		oto41268
orthoMCL	1	0.900	-	-		OOG6_102249
Panther	1	1.100	-	-	LDO	PTHR20856
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1173
SonicParanoid	1	1.000	-	-		X3081
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	16	15.800