DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Plc21C and plc-1

DIOPT Version :9

Sequence 1:	NP_995605.1	Gene:	Plc21C / 33204	FlyBaseID:	FBgn0004611	Length:	1318	Species:	Drosophila melanogaster
Sequence 2:	NP_001360638.1	Gene:	plc-1 / 181274	WormBaseID:	WBGene00004036	Length:	3554	Species:	Caenorhabditis elegans

Alignment Length:	1646	Identity:	371/1646 - (22%)
Similarity:	613/1646 - (37%)	Gaps:	542/1646 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly    11 TASVEVPQALQDGEKFIRWDDDSGTGTPVTMRVDAKGFFLYW------VDQN-NELDIL---DIA 65
            |..::|.|.||.|...|.::.|||......:|:|.....:.|      |::: .|.|:|   .::
Worm  2038 TFDLDVIQRLQHGTTVIHYEPDSGRSNLCLLRLDPSCGQINWHKISYSVNKDPKEKDVLAKVSVS 2102

  Fly    66 TIRDVRTGQYAKRP--------------KDNKLRQIVTLGPQ---------------DTLEEKTV 101
            .::.:.:|:.|..|              ::.:|:..|..|.:               .::||.:|
Worm  2103 NLQPLDSGRGAPSPMPSGRTPGTGGVGVEEGELKLSVVKGVELVDSYDIDIEAIYRRHSMEEMSV 2167

  Fly   102 TV-C----HGSDFVNMTFVNFCCTRRDIAQLWTDGL--------------------IKLAY-SLA 140
            .| |    ||....:..|:.|...:: |||.||:||                    ||..| ||.
Worm  2168 PVSCWKVSHGQLLSDNEFIYFLAPQQ-IAQFWTNGLQSVVKSLQGQQRYPDRRMLWIKNVYLSLY 2231

  Fly   141 QLNGSA-----------IMFLQKAHTKLCLQVDKS-------GRIPVKNI-----IKLFAQNKE- 181
            ::.|.:           ...|.:.:|......|.|       .:..:||:     .||...::|
Worm  2232 EITGESNCGPRPFEALQAFGLSQTNTNATRPNDSSLSSEPGGAKSRLKNLKNAMQKKLRGASREG 2296

  Fly   182 ---------------------------------------------------------DRKRV--- 186
                                                                     .|.|.
Worm  2297 SRSQSPQPHSPLVRPPSIKSQISSQSGPPGPNSPGYLLKPRGEPANSDAGDIDSIYTPRSRTPTS 2361

  Fly   187 ---------------------------------EKALDVTGL--PSGK-VDSISVSKFQFEDFYN 215
                                             :.::.|:||  |||| .....::..:|.:.:.
Worm  2362 SSYGGRSVGGRSCKSWRSRGGETPNSGSISSSGQMSIQVSGLSGPSGKEFQEKPLTLVEFAELFR 2426

  Fly   216 LYKYLTQRSEVERLFDSIVG--------------------NSKRKCMSIAQLVEFL--------- 251
            |:. ...|.::..:|:.::.                    .|:...:|.:...:||
Worm  2427 LFN-TRMRKDLRDVFNDVLSTATTPQHCPKRERDRHSPRMQSRLASVSNSYNADFLSNDFLTRNT 2490

  Fly   252 -----------NK------------------TQRDPRLN-EILYPYANPAR--------AKELIQ 278
                       ||                  |.|...|. ::|..:.|..:        |.:|||
Worm  2491 AVTSHHISEKQNKIYNALALASVNSMGGLMDTSRSSMLTPQMLRAFVNTHQMEQIDEQTAIKLIQ 2555

  Fly   279 QYEPNKFNAQKGQLSLDGFLRYLMGDDNPIMAPSKLDLC-DDMDQPMSHYFINSSHNTYLTGHQL 342
            .:||:....||.|:|.:||.|:|....|....|..::.. :|:..|:|||:||||||||||||||
Worm  2556 DHEPDGICRQKNQMSFEGFTRFLCDPVNFAFVPETIEPDEEDLRYPLSHYYINSSHNTYLTGHQL 2620

  Fly   343 TGKSSVEIYRQCLLAGCRCVELDFWNGRTEEPVIVHGYTFVPEIFAKDVLEAIAESAFKTSEYPV 407
            .|.||.|:|||.||.||||||||.|:|....|:|.||:|.|.:|..:.|:|.|.:|||.||:.||
Worm  2621 KGPSSSEMYRQVLLTGCRCVELDCWDGDDGLPLIYHGHTLVSKIGFRQVVEIIKKSAFITSDLPV 2685

  Fly   408 ILSFENHCNPRQQAKIANYCREIFGDMLLDKPLDSHPLEPNMDLPPPAMLRRKIIIKNKKK---- 468
            |||.||||:.:||||:|...:.:.||:|:...|.......:..||.|..::.||:|||||.
Worm  2686 ILSIENHCSLQQQAKMAQMFKTVLGDLLVSNFLFEADFSDSPRLPCPLQMKNKILIKNKKMIVDP 2750

  Fly   469 ------------HHHHHHHHHHKKPAQVGTPAANNKLTTANSV--------------DAKAAQQV 507
                        .......:.|:|.::     .:.:.:|.:.|              :....::|
Worm  2751 PTPLPMIERGAVQRGETQLNLHRKQSK-----NSYESSTVDEVEDDDLDEFLDDEENEEDDQEEV 2810

  Fly   508 GLSASHEDGGVTRSTANGDVATGTGTGSAAGTAGHAPPLQQIRQSSKDSTGSSDSDSSSEDESL- 571
            .:.:..||...|...|...........|..  :.|:  ::|.:.|:..:| |..:|..:|||.|
Worm  2811 QVRSEKEDSPKTSKRAEKSARNIKQQDSLC--SDHS--VEQAKPSTSKTT-SKTNDRKTEDEVLY 2870

  Fly   572 --------------PNTT-----PNL--------------------------------------- 578
                          .|.|     |.|
Worm  2871 AQLAQNAIRNQQPRKNNTGVQIAPELSDIVIYMQATKFKGFPPVDGIQSPRIMEEGPASASLSFS 2935

  Fly   579 -----PSG--NEP-PPEKAQKETEAGAEISALVNYVQPIHFSSFENAEKKNRCYEMSSFDEKQAT 635
                 ||.  |.| ||.:.:..|:...|::|        .|.....|.....||:::|.:|..|.
Worm  2936 SRARTPSNLLNTPAPPRRQRSSTQLSQELAA--------EFLGSVRANATATCYQVTSLNENAAK 2992

  Fly   636 TLLKERPIEFVNYNKHQLSRVYPAGTRFDSSNFMPQLFWNAGCQLVALNFQTLDLAMQLNLGIFE 700
            .|:|..|.:.|:|.:..|.|.||:...:|||||.|...|..|.|:|||||||.|:.|.:|..:||
Worm  2993 KLMKRHPAKCVSYTRDHLIRTYPSAKHYDSSNFNPINCWAHGMQMVALNFQTPDVIMAVNQAMFE 3057

  Fly   701 YNARSGYLLKPEFMRRSDRRL-DPFAESTVDGIIAG----TVSITVLSGQ--FLTDKRANTFVEV 758
            .:...||.|||..:......| :.|...:.|  |||    .:::|::|||  :.....|:.:||:
Worm  3058 QSGNCGYQLKPRCLWDESHLLYNKFLPLSKD--IAGHSALLLNLTIISGQHVYPNTHYASLYVEI 3120

  Fly   759 DMYGLPADTVRKKFRTKTVRDNGMNPLYDEEPFVFKKVVLPELASIRIAAYEEG--GKLIGHRVL 821
            ::.|:..|.||:|  :|.|:.|.:||:::....:  ::...:||.:|||..:.|  |:::.|||:
Worm  3121 EVIGIHNDCVREK--SKVVQRNSVNPIWNHTTQL--RIACVDLAFLRIAVCDSGQNGRVVAHRVV 3181

  Fly   822 PVIGLCPGYRHVNLRSEVGQPIALASLFLCVVVKD----YVPDDLSNFAEALANPIKYQSELEKR 882
            ||..:.||:||:.||:....||..|.:||....:.    |:.||.||....|.:.:.|:::|...
Worm  3182 PVKCIRPGFRHLPLRTPTNLPIDNAMIFLRTRFEQEEHIYLHDDDSNTYCNLEHTLAYRTDLTPN 3246

  Fly   883 DIQLSVLTDEAEALGSADEDLSKSFVFVQVGGQKKELRPVESLATSPKHRPSISAAAAMSVDVTV 947
            .....:|               |..:||        ||              |:.|.|....:||
Worm  3247 LSPTPIL---------------KKQIFV--------LR--------------ITGAFADETAITV 3274

  Fly   948 DRTDG--------------GRGEDSI--------SIVAPSIQHQHSLDQSVSTSIRQVESSQFDV 990
            ....|              |:..|.:        |:.|||  .:..::|.|
Worm  3275 HSESGSTVKTVMQQALLNAGKNADQVEEYVLIEESLPAPS--GEDPIEQRV-------------- 3323

  Fly   991 DLVLAEPLEKILDHKSVKEKRLEMEKKLESLRKKHDKEKIKIAGQKSSPLEGKKPKF-AITNKLV 1054
             |.|.||:...:...:...:|..:.||                        |..|.. |....::
Worm  3324 -LPLNEPIMDAVACWNGSMRRFVLRKK------------------------GSDPSSRAWITSII 3363

  Fly  1055 KRLSNKSLNCLSPHSEPGVEIPACPLDLGDSSEESAAADAGEDLAGGSSSLDGRTQESRLRSACR 1119
            |..::.|...:||                     |.....|...:..|:.|.||:.::
Worm  3364 KSGTSGSSTSVSP---------------------SPLTKDGHVKSASSNQLHGRSLDT------- 3400

  Fly  1120 EYTSQYRELQEKYHEAIYSAAEKVL----KTSQTGQTKQLKASLDKVTGEVMHQL-QEARRNEVK 1179
            :...::.|:.|..:....|..:..|    ..|:......|:|.:.....:::.|: .:|||:.|
Worm  3401 DAFGEHLEVTEGLNPRARSMGDTFLVCVHNVSEDQPYAILRAGIHSTAADIIRQVFVKARRSNV- 3464

  Fly  1180 NLATVHRDRDELIRMKREVASSVVERGVAERVRLKQTFDRRTDELQKQHDSVRNALAEHRSKARQ 1244
                   |..|.:.::.......:.:|   ::..|...:|.|..:..|:::|..|.:..:|..|.
Worm  3465 -------DDSEFVLVEETCDDPKLNQG---QMTPKYPNNRTTSRVLGQNENVWKAQSRWKSMGRF 3519

  Fly  1245 ILDKEAES--------RSCVS 1257
            :|:...::        ||.:|
Worm  3520 VLENRKDTVHATLEKVRSFIS 3540

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Plc21C	NP_995605.1	PH_PLC_beta	20..145	CDD:270167	41/189 (22%)
		PLN02222	213..850	CDD:177868	230/810 (28%)
		EF-hand_like	225..317	CDD:286375	28/158 (18%)
		PI-PLCc_beta	317..702	CDD:176533	151/482 (31%)
		C2_PLC_like	736..855	CDD:175974	40/122 (33%)
plc-1	NP_001360638.1	Ank_2	193..328	CDD:372319
		ANK repeat	193..219	CDD:293786
		ANK repeat	221..293	CDD:293786
		ANK repeat	295..328	CDD:293786
		B41	<1421..1598	CDD:214604
		RasGEF	1748..2019	CDD:214539
		EFh_PI-PLCepsilon	2401..2581	CDD:320033	35/180 (19%)
		EF-hand motif	2402..2429	CDD:320033	8/26 (31%)
		EF-hand motif	2435..2500	CDD:320033	5/64 (8%)
		EF-hand motif	2509..2544	CDD:320033	5/34 (15%)
		PLN02952	2526..3215	CDD:178538	221/712 (31%)
		EF-hand motif	2549..2581	CDD:320033	14/31 (45%)
		PI-PLCc_epsilon	2595..3059	CDD:176538	151/481 (31%)
		Ubiquitin_like_fold	3255..3349	CDD:391949	23/132 (17%)
		RA2_PLC-epsilon	3421..3524	CDD:340478	21/113 (19%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C160162213
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D368239at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.940