DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Pi3K21B and pik3r2

DIOPT Version :9

Sequence 1:	NP_001259817.1	Gene:	Pi3K21B / 33203	FlyBaseID:	FBgn0020622	Length:	496	Species:	Drosophila melanogaster
Sequence 2:	NP_997987.2	Gene:	pik3r2 / 404211	ZFINID:	ZDB-GENE-040309-1	Length:	724	Species:	Danio rerio

Alignment Length:	461	Identity:	159/461 - (34%)
Similarity:	224/461 - (48%)	Gaps:	89/461 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 PQAP------GSLVDPNEDELRMAPWYWGRISREEAKSILHGKPDGSFLVRDALS-MKGEYTLTL 60
            |:.|      .|:.:.::..|..|.||||.|||||....:...|||:||||||.| :.|||||||
Zfish   306 PKPPKAKAMASSVTNGSDSLLSEAEWYWGDISREEVNEKMRDTPDGTFLVRDASSKVHGEYTLTL 370

  Fly    61 MKDGCEKLIKICHMDRKYGFIETDLFNSVVEMINYYKENSLSMYNKTLDITLSNPIVRAREDEES 125
            .|.|..|||||.|...||||.|...|.||||:||:|:..||:.||..||..|..|:.:.::|:
Zfish   371 RKGGNNKLIKIFHRGGKYGFSEPLTFLSVVELINHYRHESLAQYNAKLDSHLLFPVSKYQQDQ-- 433

  Fly   126 QPHGDLCLLSNEFIRTC--QLLQNLEQNLENKR------NSFNAIREELQEKKLHQSVFGNTEKI 182
                   ::..:.|...  ||....||..|..|      ..:....:|||.|:.....|..|.||
Zfish   434 -------VVKEDSIEAVGEQLKVYHEQYQEKSREYDVLYEEYTRSSQELQMKRTAIEAFNETIKI 491

  Fly   183 FRNQIKLNESFMKAPADAPSTEAGGAGDGANAAASAAANANARRSLQEHKQTLLNLLDALQAKGQ 247
            |..|.:..|...:...:....|               .|......:|.:.:.|         |.:
Zfish   492 FEEQCETQERLSRDSIEKFRRE---------------GNDKEIERIQSNSEKL---------KSR 532

  Fly   248 VLNHYMENKKKEELLLERQ----------INALKPELQILQLRKDKYIERLKGFNLKDDDLKMIL 302
            |...: ::|:|.||.|:||          :|:|||:|  :||||           |:|..|..:.
Zfish   533 VTEIH-DSKRKLELDLKRQATDNREIDKLMNSLKPDL--IQLRK-----------LRDQYLVWLT 583

  Fly   303 QMG-----FDKW----------QQLYETVSNQPHSNEALWLLKDAKRRNAEEMLKGAPSGTFLIR 352
            |.|     .::|          ..|.:...:|.|.:|..|.:.|.||..||::|:|...||||||
Zfish   584 QKGTRQRKINEWLGIKNESEDFYSLLDDDDDQAHHDECSWYVGDIKRSYAEDLLRGKRDGTFLIR 648

  Fly   353 ARDA--GHYALSIACKNIVQHCLIYETSTGFGFAAPYNIYATLKSLVEHYANNSLEEHNDTLTTT 415
            ....  |.:|.|:..:..::||::|:|:||||||.|||:|.:||.||.||.:.||.:|||:|..|
Zfish   649 ESQTQKGSFACSVVVEGEIKHCVVYKTATGFGFAEPYNLYGSLKDLVLHYKHTSLVQHNDSLNVT 713

  Fly   416 LRWPVL 421
            |.:|||
Zfish   714 LAYPVL 719

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pi3K21B	NP_001259817.1	SH2_nSH2_p85_like	14..123	CDD:198195	57/109 (52%)
		PI3K_P85_iSH2	129..302	CDD:293063	43/190 (23%)
		iSH2_PI3K_IA_R	138..303	CDD:304922	43/182 (24%)
		SH2_cSH2_p85_like	320..422	CDD:198184	50/104 (48%)
pik3r2	NP_997987.2	SH3_PI3K_p85beta	6..79	CDD:212842
		RhoGAP	113..298	CDD:295372
		SH2_nSH2_p85_like	326..433	CDD:198195	57/106 (54%)
		PI3K_P85_iSH2	437..596	CDD:293063	45/196 (23%)
		iSH2_PIK3R2	438..598	CDD:214019	46/197 (23%)
		SH2_cSH2_p85_like	616..720	CDD:198184	50/104 (48%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170577417
Domainoid	1	1.000	93	1.000	Domainoid score	I7496
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	226	1.000	Inparanoid score	I3467
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D323393at33208
OrthoFinder	1	1.000	-	-		FOG0001513
OrthoInspector	1	1.000	-	-		otm25645
orthoMCL	1	0.900	-	-		OOG6_103810
Panther	1	1.100	-	-	O	PTHR10155
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X1082
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	11	10.900