DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Usp2 and USP4

DIOPT Version :9

Sequence 1:	NP_001285528.1	Gene:	Usp2 / 33132	FlyBaseID:	FBgn0031187	Length:	950	Species:	Drosophila melanogaster
Sequence 2:	NP_003354.2	Gene:	USP4 / 7375	HGNCID:	12627	Length:	963	Species:	Homo sapiens

Alignment Length:	720	Identity:	161/720 - (22%)
Similarity:	221/720 - (30%)	Gaps:	333/720 - (46%)

- Green bases have known domain annotations that are detailed below.

  Fly   535 NSASDSVSRSLARSPISSTLARSGTGSSSTARSVLPPMTPTSSRYWDRDSGTSRSSIGTSSALN- 598
            :.:|.:.||:...||.||....|...:|..|..   ..|.|...:   .||.||...|.|::.|
Human   231 SKSSTAPSRNFTTSPKSSASPYSSVSASLIANG---DSTSTCGMH---SSGVSRGGSGFSASYNC 289

  Fly   599 ----SSSLKHNSDDGYKTASSSRDEKSEGLCGLRNIGNTCFMNSVIQCLSHTQELT-RFLRSHHG 658
                ||.::                  .|||||.|:||||||||.:||||:|..|| .||:..:.
Human   290 QEPPSSHIQ------------------PGLCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYE 336

  Fly   659 S-----RSLSTKDQQILHEFAKLIQEMWTANVHTVTPMELKRAFSTKHRMYSDYNQQDAQEFLRF 718
            :     ..|..|. :|...:|:||::||:.....|.|...|.........:|.|.|||:||.|.|
Human   337 AEINRDNPLGMKG-EIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAF 400

  Fly   719 FLDSLHSALNSGVKGETLNIDD--NLSDNKKADLTWEWYTRHENSLVRDLFVGQLKSTLKCTTCG 781
            .||.||..||...|...|.:.|  ...|...|...||.:....:|::.|.|.|..||||.|..|.
Human   401 LLDGLHEDLNRVKKKPYLELKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECA 465

  Fly   782 NTSVTFDPFWDLSVPLP------------------------------------------------ 798
            ..|||||||..|::|||
Human   466 KVSVTFDPFCYLTLPLPLKKDRVMEVFLVPADPHCRPTQYRVTVPLMGAVSDLCEALSRLSGIAA 530

  Fly   799 ----------------------------------------------------------------- 798

Human   531 ENMVVADVYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSVDGSECVTLPVYFRERKSRPSSTS 595

  Fly   799 --------------------------------------------------------SSSRCK--- 804
                                                                    |.:.|:
Human   596 SASALYGQPLLLSVPKHKLTLESLYQAVCDRISRYVKQPLPDEFGSSPLEPGACNGSRNSCEGED 660

  Fly   805 ----------------------------------------------------------------- 804

Human   661 EEEMEHQEEGKEQLSETEGSGEDEPGNDPSETTQKKIKGQPCPKRLFTFSLVNSYGTADINSLAA 725

  Fly   805 ----------------------------------------------------LEACLDLFIREEV 817
                                                                |..|::||...|.
Human   726 DGKLLKLNSRSTLAMDWDSETRRLYYDEQESEAYEKHVSMLQPQKKKKTTVALRDCIELFTTMET 790

  Fly   818 LDGDEMPTCAKCKTRRKCTKSFTIQRFPKYLVIHLKRFSETRW--SKLSNIVEFPTSDSELNMGS 880
            |...:...|..||..::.||.|.:...||.||:||||||..|:  .||..:||||...  |||..
Human   791 LGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDTVVEFPIRG--LNMSE 853

  Fly   881 YGANSNSNVH-YSLYAISNHMGSTAGGHYVALCKHPVSRKWHEFNDNIVSDALSENHLVSSSAYI 944
            :..|.::..: |.|.|:|||.|:...|||.|..|:.::.||:.|:|:.||.| ||:.:|:.:||:
Human   854 FVCNLSARPYVYDLIAVSNHYGAMGVGHYTAYAKNKLNGKWYYFDDSNVSLA-SEDQIVTKAAYV 917

  Fly   945 LFYER 949
            |||:|
Human   918 LFYQR 922

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Usp2	NP_001285528.1	UCH	624..947	CDD:278850	136/622 (22%)
Usp2	NP_001285528.1	Peptidase_C19R	626..948	CDD:239139	135/621 (22%)
USP4	NP_003354.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..22
		Necessary for interaction with SART3. /evidence=ECO:0000269\|PubMed:20595234	27..216
		Peptidase_C19	35..923	CDD:321984	161/720 (22%)
		Nuclear export signal. /evidence=ECO:0000250\|UniProtKB:P35123	133..141
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	219..253		7/21 (33%)
		Required for USP4 activation by providing conformational flexibility between the DUSP and catalytic domains. /evidence=ECO:0000269\|PubMed:25404403	229..295		19/69 (28%)
		Regulates ubiquitin dissociation. /evidence=ECO:0000269\|PubMed:25404403	384..386		0/1 (0%)
		Necessary for interaction with RBL2. /evidence=ECO:0000250\|UniProtKB:P35123	405..407		1/1 (100%)
		Necessary for interaction with RB1 and RBL2. /evidence=ECO:0000250\|UniProtKB:P35123, ECO:0000269\|PubMed:11571652	459..463		2/3 (67%)
		Interacts with DUSP and ubiquitin-like 1 domains and is required for USP4 activation. /evidence=ECO:0000269\|PubMed:25404403	485..775		2/289 (1%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	637..699		2/61 (3%)
		Nuclear localization signal. /evidence=ECO:0000250\|UniProtKB:P35123	767..772		0/4 (0%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	928..963
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1591
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.940