DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment peng and PUM3

DIOPT Version :9

Sequence 1:	NP_523429.1	Gene:	peng / 33112	FlyBaseID:	FBgn0015527	Length:	737	Species:	Drosophila melanogaster
Sequence 2:	NP_055693.4	Gene:	PUM3 / 9933	HGNCID:	29676	Length:	648	Species:	Homo sapiens

Alignment Length:	718	Identity:	194/718 - (27%)
Similarity:	320/718 - (44%)	Gaps:	136/718 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly    43 RKFDKFGG------GAGKSFVKPGAGGAKKFDKSGPGGFKKFDKSGATGDKRLGGNKFRGKPQTQ 101
            ||..|.||      ...||..|.|..|.|:|.....|     |||  ..:|....|||..|.:.|
Human    38 RKVAKEGGPKVTSRNFEKSITKLGKKGVKQFKNKQQG-----DKS--PKNKFQPANKFNKKRKFQ 95

  Fly   102 PQAPAE---GEKQDWNKFKKEKKDLKLKRK-SAKDTYEISKEANQIHEKLR---CRRTENKDKLV 159
            |...::   .:|..|:.|||:||:||..|: |.|..|:|...|.|:.|.||   |.: |.:.||:
Human    96 PDGRSDESAAKKPKWDDFKKKKKELKQSRQLSDKTNYDIVVRAKQMWEILRRKDCDK-EKRVKLM 159

  Fly   160 EQIYKVLNVGDTISKVVKAHDTARVIQSMLKYASPALRAEISEKLLPFTVEMCQSKYAQFCVQRM 224
            ..:.|::.  ..|..:..|||:.||||..::|.:...|.:..|:|....||:.::||::..|::.
Human   160 SDLQKLIQ--GKIKTIAFAHDSTRVIQCYIQYGNEEQRKQAFEELRDDLVELSKAKYSRNIVKKF 222

  Fly   225 LKYGAPATKAKLVDSLYGHIVRLAGHSIGSGLLDSMY-QSATPNQRIYMRQEFYGDLYRKAKDSN 288
            |.||:....|:::.|..||:.::..|:..|.:::..| ..|...||..:.:|.||:.::..|.::
Human   223 LMYGSKPQIAEIIRSFKGHVRKMLRHAEASAIVEYAYNDKAILEQRNMLTEELYGNTFQLYKSAD 287

  Fly   289 VKTLSDTYKEATNMKASILGSVKANLDHVANKQ-LVDSALVHAVMLEYLRACDEDEEKL-EETVT 351
            .:||....:........|:..:|..|..:|.|: ::..:|||.|.|::....   ..|| .|.:.
Human   288 HRTLDKVLEVQPEKLELIMDEMKQILTPMAQKEAVIKHSLVHKVFLDFFTYA---PPKLRSEMIE 349

  Fly   352 AFAALVPHMLSTKEGSEAAVICFYKSTPKNRRAIIKNIKEHLLKIANHEHGHVFLISLLNALDDT 416
            |....|.::..|.:|:..|:.|.:..|||:|:.|:|.:|.::.|:||.::.|:.|::..:.:|||
Human   350 AIREAVVYLAHTHDGARVAMHCLWHGTPKDRKVIVKTMKTYVEKVANGQYSHLVLLAAFDCIDDT 414

  Fly   417 KATKKAIYDHLHGDLKALMSSPYGRRVIQWLVAPGDTTCFHPEFIRTVE--EGLAFGKKEKELRR 479
            |..|:.|...:...|.::::..|||:|:.:|::|.|......|.|..::  :|.|..||:.|:||
Human   415 KLVKQIIISEIISSLPSIVNDKYGRKVLLYLLSPRDPAHTVREIIEVLQKGDGNAHSKKDTEVRR 479

  Fly   480 KEILEQIEAPIAQSIAEDAAFWLSNSHIGLVTGDILNHIQGESYEKAASALAQVVVQPEWRISAD 544
            :|:||.|...:...:.|.|...:.:....::..|||....|:             |||.....|.
Human   480 RELLESISPALLSYLQEHAQEVVLDKSACVLVSDILGSATGD-------------VQPTMNAIAS 531

  Fly   545 --AAGPQPQDKKKPHNDVEAIIAQATKQRRKLLYVESSSDDEDEDEDEDEESDDEGDEKEQKEAA 607
              |.|..|..|     |.|..||
Human   532 LAATGLHPGGK-----DGELHIA------------------------------------------ 549

  Fly   608 ADDAEPKVKKAKKEPKKPKAKEEEPAAPLVSGIEEAGMHIVLKKILKNDGK-----REGTPFSQQ 667
                                  |.||.           |:|||.:::.|.|     |||. |::.
Human   550 ----------------------EHPAG-----------HLVLKWLIEQDKKMKENGREGC-FAKT 580

  Fly   668 LLQNLSSDVLKAWLGVNRACFVLLKLVEECPALLDDCKKAIAAERSLSQILADRK--TPGAKLLA 730
            |::::....||:|..|||...:|..|::.|.  |:...|..||.:||...|...|  :.|.::|.
Human   581 LVEHVGMKNLKSWASVNRGAIILSSLLQSCD--LEVANKVKAALKSLIPTLEKTKSTSKGIEILL 643

  Fly   731 AKL 733
            .||
Human   644 EKL 646

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
peng	NP_523429.1	Pumilio	<183..>254	CDD:277559	21/70 (30%)
peng	NP_523429.1	CPL	438..>537	CDD:285370	27/100 (27%)
PUM3	NP_055693.4	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..124		32/92 (35%)
		Nuclear localization signal. /evidence=ECO:0000269\|PubMed:21266351	106..118		5/11 (45%)
		Pumilio	162..>403	CDD:277559	67/245 (27%)
		Pumilio 1. /evidence=ECO:0000269\|PubMed:25512524	177..212		12/34 (35%)
		Pumilio 2. /evidence=ECO:0000269\|PubMed:25512524	213..248		10/34 (29%)
		Pumilio 3. /evidence=ECO:0000269\|PubMed:25512524	249..277		6/27 (22%)
		Pumilio 4. /evidence=ECO:0000269\|PubMed:25512524	289..325		7/35 (20%)
		HA-8	289..297		2/7 (29%)
		Pumilio 5. /evidence=ECO:0000269\|PubMed:25512524	326..361		10/37 (27%)
		Pumilio 6. /evidence=ECO:0000269\|PubMed:25512524	362..397		12/34 (35%)
		Pumilio 7. /evidence=ECO:0000269\|PubMed:25512524	398..435		9/36 (25%)
		CPL	434..571	CDD:285370	47/229 (21%)
		Pumilio 8. /evidence=ECO:0000269\|PubMed:25512524	436..504		22/67 (33%)
		Pumilio 9. /evidence=ECO:0000269\|PubMed:25512524	505..551		16/127 (13%)
		Pumilio 10. /evidence=ECO:0000269\|PubMed:25512524	552..596		16/55 (29%)
		Pumilio 11. /evidence=ECO:0000269\|PubMed:25512524	597..636		13/40 (33%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165147211
Domainoid	1	1.000	168	1.000	Domainoid score	I3846
eggNOG	1	0.900	-	-		E1_KOG2050
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	210	1.000	Inparanoid score	I3677
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG55991
OrthoDB	1	1.010	-	-		D503417at33208
OrthoFinder	1	1.000	-	-		FOG0004242
OrthoInspector	1	1.000	-	-		oto90207
orthoMCL	1	0.900	-	-		OOG6_102938
Panther	1	1.100	-	-	LDO	PTHR13389
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1033
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	14	13.800