Protein Alignment HSPA5 and Grp170

DIOPT Version :10

• Sequence 1: NP_005338.1
• Gene: HSPA5 / 3309
• HGNCID: 5238
• Length: 654
• Species: Homo sapiens
• Sequence 2: NP_569995.1
• Gene: Grp170 / 31215
• FlyBaseID: FBgn0023529
• Length: 923
• Species: Drosophila melanogaster
• Alignment Length: 799
• Identity: 197/799 - (24%)
• Similarity: 331/799 - (41%)
• Gaps: 175/799 - (21%)

Human     1 MKLSLVAAMLL---LLSAARAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNG-RVEIIANDQGNRI 61
            |||.|:.:.||   .||...|           |:.:|||:.:..|||...| .:||..|.:..|.
  Fly     1 MKLVLLLSALLAGIALSQGAA-----------VMSVDLGSEWMKVGVVSPGVPMEIALNRESKRK 54

Human    62 TPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVV----EK 122
            ||:.:||. :|.|.||:.|:.....:|.:.......|:|:|.::|.|....|..|:..:    |:
  Fly    55 TPAILAFR-DGTRTIGEDAQTIGIKDPNSAYGYLLDLLGKTIDNPIVDLYRKRFPYYNIVGDPER 118

Human   123 KTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDA 187
            .|..:.:.|     |..|:.||:.|.:|.|.|:.|:..:.:.:|..|:|||.||..|:|:|...|
  Fly   119 NTVVFRKSD-----TDEFSVEELVAQLLVKAKQFAQESVQQPITECVLTVPGYFGQAEREALLSA 178

Human   188 GTIAGLNVMRIINEPTAAAIAYGLDKREGEKN-----ILVFDLGGGTFDVSLLTID--------- 238
            ..:|.|.|:::||:..|.|:.||:..| ||.|     .|.:|:|......::::..
  Fly   179 AQLANLKVLQLINDYAAVALNYGVFHR-GEINETAQYFLFYDMGAYKTSAAVVSYQLVKDKQTRE 242

Human   239 -NGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYK--KKTGKDVRKDNRAVQKLRREVEKAKRALS 300
             |.|.:|:....|..|||.:...|:.::..:.:.  |||..||....||:.||.:|..:.|..||
  Fly   243 INPVVQVLGVGYDRTLGGLEIQLRLRDYLAQEFNALKKTKTDVTTSPRALAKLFKEAGRLKNVLS 307

Human   301 SQHQARIEIESFYEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGS 365
            :..:...:||:..|..||...:||.|.|:|..||:....||:::.|..|.|....|::::|.||.
  Fly   308 ANTEFFAQIENLIEDIDFKLPVTREKLEQLCEDLWPRATKPLEEALASSHLSLDVINQVILFGGG 372

Human   366 TRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLD--VCPLTLGIE- 427
            ||:|::|:.:|.... :|..:.:|.||:...||..:|..||........|:.|  :.||.:..|
  Fly   373 TRVPRVQETIKAVIK-QELGKNLNADESATMGAVYKAADLSAGFKVKKFVVKDATLFPLQVSFER 436

Human   428 ------TVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVY----EGERPLTKDNHLLGTF 482
                  .|..|...|.......|.||...|     |:.|...:.|    :.:|...::...||:.
  Fly   437 DPGDGAAVKQVKRALFALMNPYPQKKVITF-----NKHTDDFEFYVNYADLDRYSKEEIAALGSL 496

Human   483 DLTGIPPAPRGVPQ--------------IEVTFEIDVNGILRVT--------------AEDKGTG 519
            ::|.:  ..:.|.:              |:..|.:|.:||.|.|              |::..|.
  Fly   497 NVTKV--QLKQVKELLEKSKKELVDNKGIKAYFYLDDSGIFRCTGVEYVYEKQKPEDDADEDSTL 559

Human   520 NK-----NKITITNDQNRLTPEEIERMVN---------DAEKFAEED--------KKLKERIDTR 562
            :|     :|:.....:.:....|.|...|         |.||..|||        :..|:..:.:
  Fly   560 SKFGSTLSKLFTKEGEEKKDNSEQEEAANAGEEPSKSEDNEKAKEEDASKEQKSEESTKQDTEAK 624

Human   563 NE------------------------------------------------------LESYAYSLK 573
            ||                                                      ||::...::
  Fly   625 NETIKLVTVKSPVTYESQTQFVVPLVGSAYDQSVAKLAAINKAEEQRVRLESAFNALEAHIIEVQ 689

Human   574 NQIGDKEKLGGKLSSEDKETMEKAVEEKIEWL-ESHQDADIEDFKAKKKELEEIVQPIISKLYGS 637
            .:: |:|......::|:||.:........||| |..:|...|.::.|..:|:::....:::.:..
  Fly   690 QKL-DEESYAKCATAEEKEKLLAECSTLGEWLYEDLEDPKAEIYEEKLAQLKKLSNVFLARHWEH 753

Human   638 AGPPPT-----GEEDTAEK 651
            ...|..     |..|.|||
  Fly   754 EERPEAIKALKGMIDGAEK 772

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
HSPA5	NP_005338.1	Required for interaction with ELAPOR1. /evidence=ECO:0000269|PubMed:26045166	1..80		29/82 (35%)
		HSP70	30..635	CDD:394970	182/744 (24%)
		Nucleotide-binding (NBD). /evidence=ECO:0000303|PubMed:28286085	125..280		50/171 (29%)
		Interdomain linker. /evidence=ECO:0000250|UniProtKB:G3I8R9	409..419		2/11 (18%)
		Substrate-binding (SBD). /evidence=ECO:0000303|PubMed:28286085	420..500		19/104 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	633..654		6/24 (25%)
		Prevents secretion from ER. /evidence=ECO:0000255	651..654		1/1 (100%)
Grp170	NP_569995.1	ASKHA_NBD_HSP70_HYOU1	21..409	CDD:466828	122/406 (30%)
		PTZ00121	<546..917	CDD:173412	37/228 (16%)
		TAF4	<572..>627	CDD:461598	11/54 (20%)