Protein Alignment Npc1b and SCAP

DIOPT Version :9

• Sequence 1: NP_608417.2
• Gene: Npc1b / 33072
• FlyBaseID: FBgn0261675
• Length: 1254
• Species: Drosophila melanogaster
• Sequence 2: NP_788277.1
• Gene: SCAP / 35529
• FlyBaseID: FBgn0033052
• Length: 1276
• Species: Drosophila melanogaster
• Alignment Length: 561
• Identity: 117/561 - (20%)
• Similarity: 215/561 - (38%)
• Gaps: 122/561 - (21%)

  Fly   290 GAFGKTSAPSVCMPTLFGEFFY-HGFRIWGTFCAKHPVIVLALCSWAIAGLSFGIRYM----TIT 349
            |..|...|.|..:|.....|:| ||.     |.:.:|....::...||....:.:..:    ||.
  Fly    43 GVAGAAGASSTGLPASVSHFYYKHGL-----FLSSYPTCASSIAFMAILLSCYPLINIPLPGTIP 102

  Fly   350 TD---PVELWAGE--------ESQTRIEKDYFDQHFGPFYRTNQMFVKAVNQTYFTHETSNGVLN 403
            |.   |.|..:|.        .|.|..|.....:.:.|        ...|..:..|..:...:|.
  Fly   103 TKIVVPYETGSGSLSWHSLNTSSTTPQEPHPSGEPWPP--------EPQVLNSSTTDRSPPPLLP 159

  Fly   404 F---GPAFEY---------------------NF---LKEVFELQDSIMKLGMADNE-GLDKIC-Y 439
            :   .|||.|                     .|   |.|||:|.:.:.....::|: .|:..| :
  Fly   160 WAQSSPAFFYVQQITLRTSVLPWTEGMQLMDAFRAPLHEVFKLLEIVRNHQSSENKRTLEHNCLH 224

  Fly   440 APVLMAGETPTVDR------CAIQSVYGYFQHDMDRFE------NSYVDSNNYTINYLNQLEDCL 492
            ...:..|....:|:      |.:.|....:..:...|.      |:....:|...:.::..|...
  Fly   225 VDNVKRGTHGQLDQIFPEYGCLLLSPANLWTQNSQNFTRDTNILNTIFQYHNLQKSKVSAAEMLF 289

  Fly   493 RVPMMEDCFGTFGGPIEPGIAVGGMPKVAVGEDPDYMLATGLVLTFLGRNYNDESKLEPNMKWEK 557
            .:||.:..|..:             |..|......|.|...|       .:||...|: .:| ||
  Fly   290 GLPMQDTGFKRY-------------PLRARSRIIQYALTLFL-------KHNDMEYLD-TLK-EK 332

  Fly   558 LFVDFLRDYKSDRLDIAYMAERSIQDAIV---ELSEGEVSTVVISYVVMFVYVAIALGHIRSCRG 619
            |    ||.|....|..|...|.:....|.   |....|:....::::::|.||..::..|...|
  Fly   333 L----LRHYPPLPLASASAEEPTTITYIFYPGEYRMWELVPYTVAFMLVFAYVYFSVRKIDVFR- 392

  Fly   620 FLRESRIMLAIGGIVIVLASVVCSLGFWGYLDVTTTMLAIEVIPFLVLAVGVDNIFIMVHTYQRL 684
                ||.:||:..::....|:..|||...:..:|.::.:.::.|:||:.||::|..::..:...:
  Fly   393 ----SRFLLALCSVITTAGSLAMSLGLCFFFGLTISLQSKDIFPYLVILVGLENSLVITKSVVSM 453

  Fly   685 DHS---KFKTTHEAIGEAIGQVGPSILQTAGSEMACFAIGCISDMPAVKTFAMYAAIAILLDFLL 746
            |.:   |.:     :.:|:.:.|..|.:|..:|:....||..:.:|.::.|.::|.:.:|.||:|
  Fly   454 DETFDVKIR-----VAQALSKEGWHISKTLLTEITILTIGLATFVPVIQEFCIFAIVGLLSDFML 513

  Fly   747 QITAFVALMAIDEKR--------YLDGRLDMLCCVKSGGKK 779
            |:..|..::|::.||        :|...|  |.|.:..|::
  Fly   514 QMLLFSTILAMNIKRTEYTAEAKHLPKML--LSCTQGAGRQ 552

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Npc1b	NP_608417.2	2A060601	24..1220	CDD:273337	117/561 (21%)
		NPC1_N	24..270	CDD:293023
		nitrilase	<265..>331	CDD:299711	11/41 (27%)
		Sterol-sensing	623..775	CDD:289145	40/162 (25%)
SCAP	NP_788277.1	Sterol-sensing	390..542	CDD:289145	38/161 (24%)
		WD40	<878..1227	CDD:225201
		WD40	878..1185	CDD:295369
		WD40 repeat	887..942	CDD:293791
		WD40 repeat	945..983	CDD:293791
		WD40 repeat	989..1070	CDD:293791
		WD40 repeat	1079..1114	CDD:293791
		WD40 repeat	1120..1157	CDD:293791
		WD40 repeat	1162..1196	CDD:293791
		WD40 repeat	1201..1233	CDD:293791

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	56	1.000	Domainoid score	I3151
eggNOG	1	0.900	-	-		E1_KOG1933
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	2	1.900