Protein Alignment Npc1b and Scap

DIOPT Version :10

• Sequence 1: NP_608417.2
• Gene: Npc1b / 33072
• FlyBaseID: FBgn0261675
• Length: 1254
• Species: Drosophila melanogaster
• Sequence 2: NP_001094436.1
• Gene: Scap / 301024
• RGDID: 1309378
• Length: 1276
• Species: Rattus norvegicus
• Alignment Length: 672
• Identity: 132/672 - (19%)
• Similarity: 255/672 - (37%)
• Gaps: 198/672 - (29%)

  Fly   309 FFYHGFRIWGTFCAKHPVIVLALCSWAIAGLSFGIRYMTIT-TDPVELWA--------------- 357
            |:.||.     .||.:|:.::......|....:.:..:.:. |.|||...
  Rat    15 FYNHGL-----LCASYPIPIILFTGLCILACCYPLLKLPLPGTGPVEFSTPVKGYSPPPADSDHK 74

  Fly   358 -GEESQTRIEKDYFDQHFGPFYRTNQMFVKAVNQTYFTHETSNGVLNFGPAFEYNFLKEVFELQD 421
             ||.|:   :.:::..  .|.....|:|||:....:..:..:..|.. .|      |...|:|.:
  Rat    75 QGEPSE---QPEWYVG--APVAYIQQIFVKSSVSPWHRNLLAVDVFR-SP------LSRAFQLVE 127

  Fly   422 SIMKLGMADNEG---LDKICYAPVLMAGETPTVDR---------CAIQSVYGYFQHDMDRFENSY 474
            .|....:.|:.|   |:::|   :.:....|.:.:         |.:.|...::|:|.:||   :
  Rat   128 EIRNHVLRDSSGTKSLEEVC---LQVTDLLPGLRKLRSLLPEHGCLLLSPGNFWQNDWERF---H 186

  Fly   475 VDSNNYTINYLNQ-----------LEDCLRVPMMEDCFGTFGGPIE-PGIAVGGMPKVAVGEDPD 527
            .|.:  .|..::|           |:|.|           ||.|.: .|:::....:
  Rat   187 ADPD--IIGTIHQHEPKTLQTSATLKDLL-----------FGVPGKYSGVSLYTRKR-------- 230

  Fly   528 YMLATGLVLTFLGRNYNDESKLEPNMKWEKLFVDFLR------------DYKSDRLDIAYMAERS 580
             |::..:.|.|              .::...|:..||            ..:::.|...:..|
  Rat   231 -MVSYTITLVF--------------QRYHAKFLSSLRARLMLLHPSPNCSLRAENLVHVHFKE-- 278

  Fly   581 IQDAIVELSEGEVSTVVISYVVMFVYVAIALGHIRSCRGFLRESRIMLAIGGIVIVLASVVCSLG 645
                  |:...|:..:|.:|:::|.|:..:...|.     :.:|:..||:..:|.||:|::.|:|
  Rat   279 ------EIGIAELIPLVTTYIILFAYIYFSTRKID-----MVKSKWGLALAAVVTVLSSLLMSVG 332

  Fly   646 FWGYLDVTTTMLAIEVIPFLVLAVGVDNIFIM----VHTYQRLDHSKFKTTHEAIGEAIGQVGPS 706
            ......:|.|:...|:.|:||:.:|::|:.::    |.|...|: .|.:     |.:.:.....|
  Rat   333 LCTLFGLTPTLNGGEIFPYLVVVIGLENVLVLTKSVVSTPVDLE-VKLR-----IAQGLSSESWS 391

  Fly   707 ILQTAGSEMACFAIGCISDMPAVKTFAMYAAIAILLDFLLQITAFVALMAIDEKRY----LDGRL 767
            |::...:|:....||..:.:||::.|.::|.:.::.||.||:..|..:::||.:|.    |:.||
  Rat   392 IMKNVATELGIILIGYFTLVPAIQEFCLFAVVGLVSDFFLQMLFFTTVLSIDIRRMELADLNKRL 456

  Fly   768 DMLCCVKSGGKKINDEDGDGVDRPKEVGLLETLFKNFYSPFLLSKPVKVSVLLIFTVITCLSLMV 832
            ....|:.|.               |.||                :|.:....|.....|..::.:
  Rat   457 PPESCLPSA---------------KPVG----------------RPARYERQLAVRPSTPHTITL 490

  Fly   833 TPSIEKGLDQEMSMPKNSHVVKYF---RYMVDLLAMGAPVYWVLKPG-LNYSEPLQQNLICGGVE 893
            .||..:.|    .:||...|: ||   ..:...|.|...|.|:   | |.|::|
  Rat   491 QPSSFRNL----RLPKRLRVI-YFLARTRLAQRLIMAGTVVWI---GILVYTDP----------- 536

  Fly   894 CNNNSLSVQLYTQAQYPEITSL 915
                 ..::.|..||..|.:.|
  Rat   537 -----AGLRTYLAAQVTEQSPL 553

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Npc1b	NP_608417.2	2A060601	24..1220	CDD:273337	132/672 (20%)
Scap	NP_001094436.1	2A060601	<11..>466	CDD:273337	105/543 (19%)
		Loop-1. /evidence=ECO:0000250|UniProtKB:P97260	46..284		48/299 (16%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	60..81		3/23 (13%)
		Sterol-sensing	308..452	CDD:463544	39/149 (26%)
		ER export signal. /evidence=ECO:0000250|UniProtKB:P97260	447..452		0/4 (0%)
		Loop-7. /evidence=ECO:0000250|UniProtKB:P97260	535..710		6/35 (17%)
		Interaction with SREBF2. /evidence=ECO:0000250|UniProtKB:P97260	731..1276
		WD40	771..802	CDD:197651
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	834..903
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	928..958
		WD40 repeat	1008..1044	CDD:293791
		WD40	1075..>1232	CDD:475233
		WD40 repeat	1079..1113	CDD:293791
		WD40 repeat	1120..1153	CDD:293791
		WD40 repeat	1160..1194	CDD:293791