DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Npc1b and SCAP

DIOPT Version :9

Sequence 1:	NP_608417.2	Gene:	Npc1b / 33072	FlyBaseID:	FBgn0261675	Length:	1254	Species:	Drosophila melanogaster
Sequence 2:	NP_036367.2	Gene:	SCAP / 22937	HGNCID:	30634	Length:	1279	Species:	Homo sapiens

Alignment Length:	681	Identity:	142/681 - (20%)
Similarity:	263/681 - (38%)	Gaps:	198/681 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly   309 FFYHGFRIWGTFCAKHPVIVLALCSWAIAGLSFGIRYMTIT-TDPVELWAGEESQTRIEKDY--- 369
            |:.||.     .||.:|:.::....:.|....:.:..:.:. |.|||.       |...|||
Human    15 FYNHGL-----LCASYPIPIILFTGFCILACCYPLLKLPLPGTGPVEF-------TTPVKDYSPP 67

  Fly   370 ---FDQHFG------------PFYRTNQMFVKAVNQTYFTHETSNGVLNF-GPAFEYNFLKEVFE 418
               .|:..|            |.....|:|||  :..:..|:....|..| .|      |...|:
Human    68 PVDSDRKQGEPTEQPEWYVGAPVAYVQQIFVK--SSVFPWHKNLLAVDVFRSP------LSRAFQ 124

  Fly   419 LQDSIMKLGMADNEG---LDKICYAPVLMAGETPTVDR---------CAIQSVYGYFQHDMDRFE 471
            |.:.|....:.|:.|   |:::|   :.:....|.:.:         |.:.|...::|:|.:||
Human   125 LVEEIRNHVLRDSSGIRSLEELC---LQVTDLLPGLRKLRNLLPEHGCLLLSPGNFWQNDWERF- 185

  Fly   472 NSYVDSNNYTINYLNQ-----------LEDCLRVPMMEDCFGTFGGPIE-PGIAVGGMPKVAVGE 524
              :.|.:  .|..::|           |:|.|           ||.|.: .|:::....:
Human   186 --HADPD--IIGTIHQHEPKTLQTSATLKDLL-----------FGVPGKYSGVSLYTRKR----- 230

  Fly   525 DPDYMLATGLVLTFLGRNYNDESKLEPNMKWEKLFVDFLR------------DYKSDRLDIAYMA 577
                |::..:.|.|  ::|:.:            |:..||            ..:::.|...:..
Human   231 ----MVSYTITLVF--QHYHAK------------FLGSLRARLMLLHPSPNCSLRAESLVHVHFK 277

  Fly   578 ERSIQDAIVELSEGEVSTVVISYVVMFVYVAIALGHIRSCRGFLRESRIMLAIGGIVIVLASVVC 642
            |        |:...|:..:|.:|:::|.|:..:...|.     :.:|:..||:..:|.||:|::.
Human   278 E--------EIGVAELIPLVTTYIILFAYIYFSTRKID-----MVKSKWGLALAAVVTVLSSLLM 329

  Fly   643 SLGFWGYLDVTTTMLAIEVIPFLVLAVGVDNIFIM----VHTYQRLDHSKFKTTHEAIGEAIGQV 703
            |:|......:|.|:...|:.|:||:.:|::|:.::    |.|...|: .|.:     |.:.:...
Human   330 SVGLCTLFGLTPTLNGGEIFPYLVVVIGLENVLVLTKSVVSTPVDLE-VKLR-----IAQGLSSE 388

  Fly   704 GPSILQTAGSEMACFAIGCISDMPAVKTFAMYAAIAILLDFLLQITAFVALMAIDEKRY----LD 764
            ..||::...:|:....||..:.:||::.|.::|.:.::.||.||:..|..:::||.:|.    |:
Human   389 SWSIMKNMATELGIILIGYFTLVPAIQEFCLFAVVGLVSDFFLQMLFFTTVLSIDIRRMELADLN 453

  Fly   765 GRLDMLCCVKSGGKKINDEDGDGVDRPKEVGLLETLFKNFYSPFLLSKPVKVSVLLIFTVITCLS 829
            .||....|:.|.               |.||                :|.:....|.....|..:
Human   454 KRLPPEACLPSA---------------KPVG----------------QPTRYERQLAVRPSTPHT 487

  Fly   830 LMVTPSIEKGLDQEMSMPKNSHVVKYF---RYMVDLLAMGAPVYWVLKPG-LNYSEPLQQNLICG 890
            :.:.||..:.|    .:||...|| ||   ..:...|.|...|.|:   | |.|::|       .
Human   488 ITLQPSSFRNL----RLPKRLRVV-YFLARTRLAQRLIMAGTVVWI---GILVYTDP-------A 537

  Fly   891 GVECNNNSLSVQLYTQAQYPEITSLARPASS 921
            |:   .|.|:.|:..|:...|......|..|
Human   538 GL---RNYLAAQVTEQSPLGEGALAPMPVPS 565

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Npc1b	NP_608417.2	2A060601	24..1220	CDD:273337	142/681 (21%)
		NPC1_N	24..270	CDD:293023
		nitrilase	<265..>331	CDD:299711	6/21 (29%)
		Sterol-sensing	623..775	CDD:289145	43/159 (27%)
SCAP	NP_036367.2	Loop-1. /evidence=ECO:0000250\|UniProtKB:P97260	46..284		54/302 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	60..80		5/19 (26%)
		Sterol-sensing	308..457	CDD:289145	41/154 (27%)
		ER export signal. /evidence=ECO:0000250\|UniProtKB:P97260	447..452		0/4 (0%)
		Loop-7. /evidence=ECO:0000250\|UniProtKB:P97260	535..710		9/41 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	579..615
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	668..696
		Interaction with SREBF2. /evidence=ECO:0000250\|UniProtKB:P97260	731..1279
		WD40	<771..813	CDD:295369
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	811..904
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	931..962
		WD40	939..>1235	CDD:225201
		WD40 repeat	959..1005	CDD:293791
		WD40 repeat	1011..1047	CDD:293791
		WD40	1078..>1235	CDD:295369
		WD40 repeat	1082..1116	CDD:293791
		WD40 repeat	1123..1157	CDD:293791
		WD40 repeat	1163..1197	CDD:293791
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG1933
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.810