DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Npc1b and Npc1

DIOPT Version :9

Sequence 1:	NP_608417.2	Gene:	Npc1b / 33072	FlyBaseID:	FBgn0261675	Length:	1254	Species:	Drosophila melanogaster
Sequence 2:	NP_032746.2	Gene:	Npc1 / 18145	MGIID:	1097712	Length:	1277	Species:	Mus musculus

Alignment Length:	1294	Identity:	488/1294 - (37%)
Similarity:	718/1294 - (55%)	Gaps:	119/1294 - (9%)

- Green bases have known domain annotations that are detailed below.

  Fly    12 AGAWSQSAEQLGCIWYGQSHM-IGAHWVNKGDTNPARPLNSPTSEAIFAKRCPMLYKEYKGESGE 75
            |..:|||     |:|||:..: .|....|...:.|.:||.....:.: .:.||.|:        .
Mouse    18 AQVFSQS-----CVWYGECGIATGDKRYNCKYSGPPKPLPKDGYDLV-QELCPGLF--------F 68

  Fly    76 DELSLCCDAAQIETMESGLSQADGVFSRCPTCTRNMALTVCAMTCAKNHTLFL------------ 128
            |.:|||||..|::|::|.|.......||||:|..|:....|.:||:.:.:.||
Mouse    69 DNVSLCCDIQQLQTLKSNLQLPLQFLSRCPSCFYNLMTLFCELTCSPHQSQFLNVTATEDYFDPK 133

  Fly   129 TAYNDTNDAGVDYVKYIDYRLTDDTVSKIYNSCIGIQHTQTGRPAMDLGCGSYNAKTCNYRRWYE 193
            |..|.||      ||.::|.:.....:.:||:|..::...:...|:.|.||. :|:.||...|.|
Mouse   134 TQENKTN------VKELEYFVGQSFANAMYNACRDVEAPSSNEKALGLLCGR-DARACNATNWIE 191

  Fly   194 FMGDVSGDYVPFQINYKWSEDAEEGSNEIYLDLSPLK-----CGESYED-SYACACIDCEESCPL 252
            :|.:......||.|...:|:.:       .|.:.|::     |.||.:: :..|:|.||...|..
Mouse   192 YMFNKDNGQAPFTIIPVFSDLS-------ILGMEPMRNATKGCNESVDEVTGPCSCQDCSIVCGP 249

  Fly   253 TDAPTGPDELWKIAGLYGVTFILALIIACALSFFIFWG------------------------AFG 293
            ...|..|...|:|.||..:..|:.:.....|  |:|:|                        ||.
Mouse   250 KPQPPPPPMPWRIWGLDAMYVIMWVTYVAFL--FVFFGALLAVWCHRRRYFVSEYTPIDSNIAFS 312

  Fly   294 KTSA----PSVCMP--TLFGEFFYHGFRIWGTFCAKHPVIVLALCSWAIAGLSFGIRYMTITTDP 352
            ..|:    .|.|.|  ..|.:.....|..||.||.::|..::......|...|.|:.::.:||:|
Mouse   313 VNSSDKGEASCCDPLGAAFDDCLRRMFTKWGAFCVRNPTCIIFFSLAFITVCSSGLVFVQVTTNP 377

  Fly   353 VELWAGEESQTRIEKDYFDQHFGPFYRTNQMFVKAVNQTYFTHE--TSNGVLNFGPAFEYNFLKE 415
            ||||:...||.|:||:|||:|||||:||.|:.::|.|.:...:|  .:...:.|||......|.:
Mouse   378 VELWSAPHSQARLEKEYFDKHFGPFFRTEQLIIQAPNTSVHIYEPYPAGADVPFGPPLNKEILHQ 442

  Fly   416 VFELQDSIMKLGMADNE---GLDKICYAPVLMAGETPTVDRCAIQSVYGYFQHDMDRFENSYVDS 477
            |.:||.:|..:..:.|.   .|..||.||:     :|....|.|.||..|||:.....::...|.
Mouse   443 VLDLQIAIESITASYNNETVTLQDICVAPL-----SPYNKNCTIMSVLNYFQNSHAVLDSQVGDD 502

  Fly   478 NNYTINYLNQLEDCLRVP-MMED-------CFGTFGGPIEPGIAVGGMPKVAVGEDPDYMLATGL 534
            .....:|......|:|.| .:.|       |.||||||:.|.:.:||.      :|.:|..||.|
Mouse   503 FYIYADYHTHFLYCVRAPASLNDTSLLHGPCLGTFGGPVFPWLVLGGY------DDQNYNNATAL 561

  Fly   535 VLTFLGRN-YNDESKLEPNMKWEKLFVDFLRDYKSDRLDIAYMAERSIQDAIVELSEGEVSTVVI 598
            |:||...| |||..:|:....|||.|:.|:::||:..|.|::.|||||:|.:...|..:|.||:|
Mouse   562 VITFPVNNYYNDTERLQRAWAWEKEFISFVKNYKNPNLTISFTAERSIEDELNRESNSDVFTVII 626

  Fly   599 SYVVMFVYVAIALGHIRSCRGFLRESRIMLAIGGIVIVLASVVCSLGFWGYLDVTTTMLAIEVIP 663
            ||||||:|:::|||||:||...|.:|:|.|.|.||:|||:||.||||.:.|:.:..|::.|||||
Mouse   627 SYVVMFLYISLALGHIQSCSRLLVDSKISLGIAGILIVLSSVACSLGIFSYMGMPLTLIVIEVIP 691

  Fly   664 FLVLAVGVDNIFIMVHTYQRLDHSKFKTTHEAIGEAIGQVGPSILQTAGSEMACFAIGCISDMPA 728
            |||||||||||||:|.||||.:..:.:|..:.:|..:|:|.|::..::.||.:.|..|.:|.|||
Mouse   692 FLVLAVGVDNIFILVQTYQRDERLQEETLDQQLGRILGEVAPTMFLSSFSETSAFFFGALSSMPA 756

  Fly   729 VKTFAMYAAIAILLDFLLQITAFVALMAIDEKRYLDGRLDMLCCVKSGGKKINDEDGDGVDRPKE 793
            |.||:::|.:|:|:|||||||.||:|:.:|.||.....||:||||:..      :||.|....:
Mouse   757 VHTFSLFAGMAVLIDFLLQITCFVSLLGLDIKRQEKNHLDILCCVRGA------DDGQGSHASE- 814

  Fly   794 VGLLETLFKNFYSPFLLSKPVKVSVLLIFTVITCLSLMVTPSIEKGLDQEMSMPKNSHVVKYFRY 858
             ..|...|||:::|.||...::..|:.:|..:...|:.|...::.||||.:|||.:|:|:.||:.
Mouse   815 -SYLFRFFKNYFAPLLLKDWLRPIVVAVFVGVLSFSVAVVNKVDIGLDQSLSMPNDSYVIDYFKS 878

  Fly   859 MVDLLAMGAPVYWVLKPGLNYSEPLQQNLICGGVECNNNSLSVQLYTQAQYPEITSLARPASSWL 923
            :...|..|.|||:||:.|.|||....||::|||:.|:|:||..|::..|:....|.:....|||:
Mouse   879 LAQYLHSGPPVYFVLEEGYNYSSRKGQNMVCGGMGCDNDSLVQQIFNAAELDTYTRVGFAPSSWI 943

  Fly   924 DDYIDWLA-ISDCCK-YNVTTGGFCSSNSKSEDCLPCERGFTENGLRPDAETFNKYIPYFLFDLP 986
            |||.||:: .|.||: ||| |..||:::.....|:.|.....|...||..:.|.|::|.||.|.|
Mouse   944 DDYFDWVSPQSSCCRLYNV-THQFCNASVMDPTCVRCRPLTPEGKQRPQGKEFMKFLPMFLSDNP 1007

  Fly   987 DAECAKAGRASYADAVIYTIDDVGMSTVQDSYFMQYSTTSTTSEEFYSQLREVRRISGEINAMFK 1051
            :.:|.|.|.|:|..||....||   :.:..:|||.|.|...||.::...:::.|.|:..|....:
Mouse  1008 NPKCGKGGHAAYGSAVNIVGDD---TYIGATYFMTYHTILKTSADYTDAMKKARLIASNITETMR 1069

  Fly  1052 ENNVDAEIFAYCVFYIYYEQYLTIWGDAMFSLGMSLVAIFLVTLLITGLDITSTFIVLFMVICIL 1116
            ....|..:|.|.|||::|||||||..|.:|:|.:||.:||||||::.|.::.|..|:...:..||
Mouse  1070 SKGSDYRVFPYSVFYVFYEQYLTIIDDTIFNLSVSLGSIFLVTLVVLGCELWSAVIMCITIAMIL 1134

  Fly  1117 INMLGMMWAWSINLNAISLVNLVVCVGIGVEFVAHIVRSFKRA-EGTAQERARHSLNVTGSSVLS 1180
            :||.|:||.|.|:|||:||||||:..||.|||.:||.|:|..: :|:...||..:|...||||.|
Mouse  1135 VNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTMSTKGSRVSRAEEALAHMGSSVFS 1199

  Fly  1181 GITLTKFAGIVVLGFSNSQIFQVFYFRMYLGIVLIGAAHGLILLPVLLSLLGPPQKLAR 1239
            |||||||.|||||.|:.||||::|||||||.:||:||.||||.||||||.:||....|:
Mouse  1200 GITLTKFGGIVVLAFAKSQIFEIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAK 1258

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Npc1b	NP_608417.2	2A060601	24..1220	CDD:273337	471/1261 (37%)
		NPC1_N	24..270	CDD:293023	73/264 (28%)
		nitrilase	<265..>331	CDD:299711	21/95 (22%)
		Sterol-sensing	623..775	CDD:289145	78/151 (52%)
Npc1	NP_032746.2	NPC1_N	23..267	CDD:293023	75/271 (28%)
		2A060601	25..1251	CDD:273337	481/1273 (38%)
		Important for cholesterol binding and cholesterol transfer from NPC1 to liposomes. /evidence=ECO:0000250	175..205		11/30 (37%)
		Sterol-sensing	649..802	CDD:289145	78/152 (51%)
		Di-leucine motif. /evidence=ECO:0000305	1274..1277
		Required for location in lysosomes. /evidence=ECO:0000250\|UniProtKB:O15118	1274..1277
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG1933
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0	Normalized mean entropy	S1850
OMA	1	1.010	-	-		QHG55095
OrthoDB	1	1.010	-	-		D31089at33208
OrthoFinder	1	1.000	-	-		FOG0001659
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_101370
Panther	1	1.100	-	-	O	PTHR45727
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X943
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	11	10.740