Protein Alignment Npc1b and ncr-2

DIOPT Version :9

• Sequence 1: NP_608417.2
• Gene: Npc1b / 33072
• FlyBaseID: FBgn0261675
• Length: 1254
• Species: Drosophila melanogaster
• Sequence 2: NP_498813.1
• Gene: ncr-2 / 176165
• WormBaseID: WBGene00003562
• Length: 1274
• Species: Caenorhabditis elegans
• Alignment Length: 1323
• Identity: 335/1323 - (25%)
• Similarity: 580/1323 - (43%)
• Gaps: 211/1323 - (15%)

  Fly    39 NKGDTNP---ARPLNSPTSEAIFAKRCPMLYKEYKGESGEDELSLCCDAAQIETMESGLSQADGV 100
            ||....|   .|.|| ||..|.|.|.|..|.:|      ||:..:||...|::.|...:|.|..:
 Worm    48 NKSTYLPITVTRSLN-PTYMARFEKYCSYLVQE------EDKAQVCCTELQLKGMTDRISNAATI 105

  Fly   101 FSRCPTCTRNMALTVCAMTCAKNHTLFLTAYNDTNDAGVDYVKYIDYRLTDDTVSKIYNSCIGIQ 165
            ...||:|..|.|...|..||:.:.:.|:.....|....|  |..:::::..|.|..:|.||   :
 Worm   106 LGSCPSCFDNFAKLWCQFTCSPDQSKFMKVMETTGPKNV--VVKMEFKVNRDFVEGLYESC---R 165

  Fly   166 HTQTGRPAMDLGCGSYNAKTCNYRRWYEFMGDVS-GDYVPFQINYKWSEDAEEGSNEIYLDLSPL 229
            ||.... .:.|...|...|. ::..:|.|||..: ...:|....:::|    ...|.:.:..:|
 Worm   166 HTWFAN-GLALRLMSLGGKV-SFENFYGFMGTKNLAQSIPINTEFQFS----RMKNAMNIPTTP- 223

  Fly   230 KCGESYEDSY-ACACIDCEESC-PLTDA------------PTGPDELW--KIAGLYGVTFILALI 278
             |.:|..... ||..|||..:. .|.|.            |..||..|  ||.|...:|.:|..|
 Worm   224 -CHKSAGPKVPACGAIDCPTNAHQLVDISKVEHLGTKVFHPHFPDFEWLLKICGCLALTVLLVFI 287

  Fly   279 I--ACALSFFIFWGAFGKTSAPS---VCMPTLFG----EFFYHGFRIWGTFCA-------KHPVI 327
            :  :|           .:.|||:   .|...| |    |..:.|      .||       |||:|
 Worm   288 LKYSC-----------HRRSAPNGEDGCYVDL-GKGNLEVQFEG------LCARYANAVIKHPLI 334

  Fly   328 VLAL--------CSWAIAGLSFGIRYMTITTDPVELWAGEESQTRIEKDYFDQHFGPFYRTNQMF 384
            .::|        ||.     :|....:|.:.|.|....||   ||..:..|...|||.:|..|:|
 Worm   335 FVSLGLIVAAACCSG-----NFKFHSLTHSVDQVSAADGE---TRRNEKKFIHSFGPNHRIEQIF 391

  Fly   385 VKAVNQTYFTHETSNGVLNFGPAFEYNFLKEVFELQDSIMKLGMA---DNEGLDKICYAPVLMAG 446
            :.       ...|:..:.|. |.||     |:|:|..:|..|...   .:..||.|||.|:   |
 Worm   392 IN-------LPPTTKSMFNM-PLFE-----EMFQLVGNIQNLTACYGNSSVKLDDICYKPI---G 440

  Fly   447 ETPTVDRCAIQSVYGYFQHDMDRFENS---YVDSNNYTINYLNQLEDCLRVPMM------EDCFG 502
            :.   ..|||.|...|||:....|||:   .:|...:...:...|:.|:|.|:.      ..|||
 Worm   441 KN---HGCAIMSPTNYFQNKWTNFENAGPPTIDDEIFDDQHWEHLKYCIRNPLTVSTYSEMSCFG 502

  Fly   503 TFGGPIEPGIAVGGMPKVAVGEDPDYMLATGLVLTFLGRNYNDESKLEPNMKWEKLFVDFLRDYK 567
            .|.|||:|.:..||..:...|.:. |..|..:::|.|.|...|::     :.||..|::.:..|:
 Worm   503 EFSGPIDPILVFGGSNESIKGAEM-YYTARTIMITVLIRGPEDQA-----IAWETAFLNMMSRYE 561

  Fly   568 SDRLDIAYMAERSIQDAIVELSEGE--VSTVVISYVVMFVYVAIALGH--IRSCRGFLRESRIML 628
            ....:..:|.|.|:.:.|....|.:  ||.:..:.|:::|...:.:.|  ..|....|...::::
 Worm   562 MKHANFTFMTETSVAEEIHTAVETDKIVSVIACAAVLIWVITMLGINHWPESSILSALVHHKLLI 626

  Fly   629 AIGGIVIVLASVVCSLGFWGYLDVTTTMLAIEVIPFLVLAVGVDNIFIMVHTYQRLDHS------ 687
            :|..::|.:.||.||:|.:....|..|..||.|:.|::..:|::.||:::.|:|...|.
 Worm   627 SISAVMISVISVWCSIGMFSLFGVHATDNAIVVLFFVITCLGINRIFVIIRTFQANGHCYGLPNI 691

  Fly   688 KFKTTHEAIGEAIGQVGPSILQTAGSEMAC-FAIGCI-----SDMPAVKTFAMYAAIAILLDFLL 746
            .::..:..|...:.:..|.:|..:.....| |..|.:     ..||||:.||.:|.:|||:|...
 Worm   692 SYREMNHRISNVMRRSIPIVLTNSLICSTCLFLAGGVLPYVSVSMPAVEVFARHAGLAILMDTAF 756

  Fly   747 QITAFVALMAIDEKRYLDGRLDMLCCVK-SGGKKIN--DEDGDG-----VDRPKEVGLLETLFKN 803
            .:...:.|...|.:|.:.|:.::....: |...|||  .|..||     ||          .||.
 Worm   757 YLLVMLPLFQYDARREMSGKCEIWPWYELSNESKINLCMEAVDGNLRSPVD----------WFKL 811

  Fly   804 FYSPFLLSKPVKVSVLLIFTVITCLSLMVTPSIEKGLDQEMSMPKNSHVVKYFRYMVDLLAMGAP 868
            ..:|.||.|..::.:...|.|...::...|..:|.|.:|.|:..:.|::.|:|:.|.:.|.:|.|
 Worm   812 AIAPLLLKKICRIWIATFFFVSLIIACYCTLCLEFGFNQVMAFSETSYLTKHFQNMNENLNIGPP 876

  Fly   869 VYWVLKPGLNYSEPLQQNLICGGVECNNNSLSVQLYTQAQYPEI---TSLARPASSWLDDYIDWL 930
            :::|::..:.:.:|..||..|....|::||:..::.:.| |.|.   ..|....:.|||.|:.::
 Worm   877 LWFVVEGDVKWHDPKMQNKFCTLAGCDDNSMGNKIRSLA-YAENYKGNYLHGDVNIWLDSYLQFM 940

  Fly   931 -AISDCCKYNVTTGGFCSSNSKSEDCLPCERGFTENGLRPDAETFNKYIPYFLFDLPDAECAKAG 994
             ....|||.:...  ||.. |.:..|..|......:....:.| |.:.:.:||...|..:||..|
 Worm   941 HPRGSCCKMDGKQ--FCDP-SNATHCSSCSSSSVASLTTTEYE-FYRNLHHFLETPPSIQCAHGG 1001

  Fly   995 RASYADAVIYTIDDVGMSTVQDSYFMQYSTTSTTSE--EFYSQLREVRRISGEINAMFKENNVDA 1057
            .|....|:..|.:    ..:|.:||..:......|:  :.|...|..:.::.:|....:...|
 Worm  1002 MALAKPAINLTRN----GKIQSAYFSTFFKKLNLSDSIQLYDAWRFAKYLADDIERELEIPGV-- 1060

  Fly  1058 EIFAYCVFYIYYEQYLTIWGDAMFSLGMSLVAIFLVTL-LITGLDITSTFIVLFMVICILINMLG 1121
            :::.|..|:.|||||||: ...:::|.:.::.:..||: |...:::..:.:.:|:::...::::.
 Worm  1061 KVYVYSTFFPYYEQYLTL-STTVYTLVVLVLFVAFVTISLFLRVNLAGSLVTVFVLLSSYLHLME 1124

  Fly  1122 MMWAWSINLNAISLVNLVVCVGIGVEFVAHIVRSFKRA-EGTAQERARHSLNVTGSSVLSGITLT 1185
            .|:...|.:|.:|::|:.:.:||.|||...::..|..: :...:|||..:|...||:.||||
 Worm  1125 WMYLLGITVNVVSVINMAMSLGIAVEFFGQMLHGFYNSKKPKREERAFAALVSNGSTTLSGI--- 1186

  Fly  1186 KFAGIVV----LGFSNSQIFQVFYFRMYLGIVLIGAAHGLILLPVLLSLLG-------------- 1232
             |..|::    |.|::|::...::....:||.|:.|.||::.:|.||::.|
 Worm  1187 -FPAIMITAGCLSFADSRVLITYFCNQLVGIGLVCAVHGVVYMPTLLAIFGSDFYQNVSSEEEST 1250

  Fly  1233 --------PPQKLARSSGAEPTA 1247
                    ||...:.:|....|:
 Worm  1251 DEAELQDTPPSTTSSTSSTSETS 1273

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Npc1b	NP_608417.2	2A060601	24..1220	CDD:273337	325/1272 (26%)
		NPC1_N	24..270	CDD:293023	68/250 (27%)
		nitrilase	<265..>331	CDD:299711	20/81 (25%)
		Sterol-sensing	623..775	CDD:289145	40/164 (24%)
ncr-2	NP_498813.1	NPC1_N	29..242	CDD:293023	59/213 (28%)
		2A060601	85..1236	CDD:273337	314/1240 (25%)
		MMPL	619..783	CDD:304422	41/163 (25%)
		MMPL	<1107..1236	CDD:304422	36/132 (27%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	111	1.000	Domainoid score	I3908
eggNOG	1	0.900	-	-		E1_KOG1933
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0	Normalized mean entropy	S1850
OMA	1	1.010	-	-		QHG55095
OrthoDB	1	1.010	-	-		D31089at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm4779
orthoMCL	1	0.900	-	-		OOG6_101370
Panther	1	1.100	-	-	O	PTHR45727
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X943
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	11	10.740