DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment HERC2 and Magi3

DIOPT Version :9

Sequence 1:	NP_608388.2	Gene:	HERC2 / 33035	FlyBaseID:	FBgn0031107	Length:	4912	Species:	Drosophila melanogaster
Sequence 2:	NP_001152826.1	Gene:	Magi3 / 99470	MGIID:	1923484	Length:	1476	Species:	Mus musculus

Alignment Length:	1813	Identity:	341/1813 - (18%)
Similarity:	544/1813 - (30%)	Gaps:	616/1813 - (33%)

- Green bases have known domain annotations that are detailed below.

  Fly  2102 HYLSLLEQPAGNEAGVYRQLHCLRLLQLILAQWGAE-----EEPRMPALVHQLFATLGRIALHCP 2161
            |:||.:::.|.:.||....|             |||     |....|        .|||:.....
Mouse    10 HWLSKVQECAVSWAGPPGDL-------------GAEIRGGAERGEFP--------YLGRLRDEAG 53

  Fly  2162 GDASLLPTAEGKA----RVLLTASH---SGSVAEELVALLR------RLHTLPSWNPVINSFLAQ 2213
            |.........|||    .|||..:.   ||....:.:|::|      ||.|:.. ..|||.
Mouse    54 GGGGTCCVVSGKAPSPGDVLLEVNGTPVSGLTNRDTLAVIRHFREPIRLKTVKP-GKVINK---- 113

  Fly  2214 KLCVAAELLAEQSHSTALDSEQVFVLGVLGAMGGHDLRPRVGLHCFHEGSHMVIASFTPKGRCLL 2278
                                               |||..:.|. |.:||
Mouse   114 -----------------------------------DLRHYLSLQ-FQKGS--------------- 127

  Fly  2279 APGGVGSGVGFVKVQLPAVMPHLDHTVFSLSRLPMNEMLLNAWTVLLYGPAPELRELPSSADGRL 2343
                                  :||   .|.::..:.:.|.  |:.....||...|:|.     :
Mouse   128 ----------------------IDH---KLQQVIRDNLYLR--TIPCTTRAPRDGEVPG-----V 160

  Fly  2344 DLALLRAQQLQMAVLHTNGVLYRHQVALRRILKQRAPGSIYASPDEPDRSDAESQQPGEQ---DQ 2405
            |...:..:|.:  .|..:|.|         :......|:.|.:|..|.........|.:|   |.
Mouse   161 DYNFISVEQFK--ALEESGAL---------LESGTYDGNFYGTPKPPAEPSPFQPDPVDQVLFDN 214

  Fly  2406 QLSSGSGQEPQLLIQCILLRATQASPVKACYSYMDLATAALNCIQSLATQAHQELSE--GGGVPP 2468
            :..:.|.::          |.|..|.::.    ||         .||..:...|..|  .|....
Mouse   215 EFDTESQRK----------RTTSVSKMER----MD---------SSLPEEEEDEDKEAVNGSGSM 256

  Fly  2469 NGRALSSPPQPTMVHGVPVYNVA------RKEQKPSEQVEQKSK-WPAAATDAQLIGQIMEMGFT 2526
            ..|.:.|......:..||.||..      |......|.:|...| |..|.||.   |.|..:...
Mouse   257 ETREMHSETSDCWMKTVPSYNQTNSSMDFRNYMMRDENLEPLPKNWEMAYTDT---GMIYFIDHN 318

  Fly  2527 RRTVELALKQLSLQA---------------EIMPTPEQIVQWI--LEHPDVCANTIEE-DTLPLA 2573
            .:|......:|..:|               |.:..|:....::  |.......|.:|| ......
Mouse   319 TKTTTWLDPRLCKKAKAPEDCEDGELPYGWEKIEDPQYGTYYVDHLNQKTQFENPVEEAKRKKQL 383

  Fly  2574 SSASSHDPEADSD----NECPS--SNSTTSSSTSSDTVEGQPMAVSGPAPPVKFESRKDFQTADL 2632
            ..|..|..:.|.:    ...||  ......:|....|: |....:.|...|.:|
Mouse   384 GQAEIHSAKTDVERAHFTRDPSQLKGVLVRASLKKSTM-GFGFTIIGGDRPDEF----------- 436

  Fly  2633 YALYVRGLVRPGMTVRCCRDFEEIKQGDMGTVLIVDTEGLHDLNVQVDWRNHGSTYWVCFVHIEL 2697
              |.|:.:::.|...:    ..:|..||    :|||..|...|         |.|      |.::
Mouse   437 --LQVKNVLKDGPAAQ----DGKIAPGD----VIVDINGNCVL---------GHT------HADV 476

  Fly  2698 VEAAQ------------THHQPRP----PPIA--VGARVRLRTSSLRYGMLC----PLRLGR--- 2737
            |:..|            ....|.|    .|:.  |.|...:...||..|..|    ..:||.
Mouse   477 VQMFQLVPVNQYVNLTLCRGYPLPDDSEDPVVDIVAATPVINGQSLTKGETCMNTQDFKLGAMVL 541

  Fly  2738 ------------------SQGSSAIGVVSSVRSKQ---LTVDFPDQPAWQGHINEVELVASQPTS 2781
                              |:.|.....::|..|.|   :|:.....|...|.     .:|..||.
Mouse   542 DQNGKSGQILASDRLNGPSESSEQRASLASSGSSQPELVTIPLIKGPKGFGF-----AIADSPTG 601

  Fly  2782 ATLPSLGDS--CSQMPPSDLIED-WSRCIRSLTVSSNEAAAKHLLNGSNQPWQSCSSGPCRHWIR 2843
            ..:..:.||  |..:...|:|:: :.:.:::||........|....|::.|......|||.
Mouse   602 QKVKMILDSQWCQGLQKGDIIKEIYHQNVQNLTHLQVVEVLKQFPVGADVPLLILRGGPCS---- 662

  Fly  2844 LELHDRILVHSLTLKVSPEDHSHMPSLLEIRVGDCVDSLKEYTWIPVPAGASRVLLMQQVPTYYP 2908
                     .:.|.|...:...:..||         :::.|.  ||.|......::....|...|
Mouse   663 ---------PTKTAKTKTDTKENSGSL---------ETINEP--IPQPMPFPPSIIRSGSPKLDP 707

  Fly  2909 WVEVVVKQCQNNGIQCKIHGIKFVGRRQQPDLQHILANAQFLASEYS-------AGVGPGSTAGA 2966
             .||.:|.             |.:...:.|:.:.:   ..||..:.|       .|.||..:...
Mouse   708 -SEVYLKS-------------KTLYEDKPPNTKDL---DVFLRKQESGFGFRVLGGDGPDQSIYI 755

  Fly  2967 GAV---STSHEEAAAAPEQDLPCT--VMVWGLNDKEQLGGLKGSKVKVPTFSQTISRLRPIHIAG 3026
            ||:   ..:.::.......:|.|.  :.|.|.:.|:.| .|..:..:......|:.|    .|..
Mouse   756 GAIIPLGAAEKDGRLRAADELMCIDGIPVKGKSHKQVL-DLMTTAARNGHVLLTVRR----KIFY 815

  Fly  3027 GSKSLFIVSQDGKVYACGEGTNGRLGLGVTHNVPLPHQLPVLRQYVVKKVAVHSGGKHALALTLD 3091
            |.|.    .:|....|..:..:.||...         :||           ..|..:.|..:||.
Mouse   816 GEKQ----PEDESHQAFSQNGSPRLNRA---------ELP-----------TRSAPQEAYDVTLQ 856

  Fly  3092 GKVFSWGEGEDGKLGHGNRTTLDKP----------RLVEALRAKKIRDVACG----SSHSAAISS 3142
            .|       |:...|....|:..||          |:::...|.:     ||    ..|.:|::.
Mouse   857 RK-------ENEGFGFVILTSKSKPPPGVIPHKIGRVIDGSPADR-----CGGLKVGDHISAVNG 909

  Fly  3143 QGELYTWGLGEYGRLGHGDNTTQL-KPKLVT-------------------------ALAGRRVVQ 3181
            |..:         .|.| ||..|| |...||                         ||..|.:.|
Mouse   910 QSIV---------DLSH-DNIVQLIKDAGVTVTLTVVAEEEHHGPPSGTNSARQSPALQHRPMGQ 964

  Fly  3182 VACGSRDAQTLALTEDGAV---------FSWGD------GDFGKLGRGGSEGSDT----PHEIER 3227
            ..........:||  :|.:         .||.|      .|...:...||..:.:    |.|:||
Mouse   965 AQANHIPGDRIAL--EGEIGRDVCSSYRHSWSDHKHLAQPDTAVISVVGSRHNQSLGCYPVELER 1027

  Fly  3228 -LSGIGVVQIECGAQFSLALTRAGEVWTWG--------KGDYYRLG--HGGDQHVR-KPQPIGGL 3280
             ..|.|         |||   |.|:.:..|        .|...:.|  |.|||.|. ..:|..|:
Mouse  1028 GPRGFG---------FSL---RGGKEYNMGLFILRLAEDGPAIKDGRIHVGDQIVEINGEPTQGI 1080

  Fly  3281 RGRRVIH-VAVGALHCLAV--TDAGQVYAWGDNDHGQQGSGNTFVNKKPALVIGLDAVFVNRVAC 3342
            ...|.|. :..|....|.:  ...|.:...||.|.....|.|...:::|.             ..
Mouse  1081 THTRAIELIQAGGNKVLLLLRPGTGLIPDHGDWDTNSPSSSNVIYDEQPP-------------PL 1132

  Fly  3343 GSSHSIAWGLPNASSDEEKRGPVPFSSTRDPLGGSSLGIYEAETMQTLKQEAKPLNQS------- 3400
            .||||       ||:.||...|    :|.|.|....:.....|...|::::...||.|
Mouse  1133 PSSHS-------ASTFEESHVP----ATEDSLTRVQICEKAEELKDTVQEKKSTLNGSQPEMKYQ 1186

  Fly  3401 SLSESLALETPAARQAALGHVLRAMSILQARQLIVAALTSHSKVNFKERGAVGGEEDHL--IGGP 3463
            |:.::::.:.|   ....||  ...|.|:...    .:|...:....::......|:||  |..|
Mouse  1187 SVHKTMSKKDP---PRGSGH--GEKSRLKGEN----GVTRRGRSASPKKSVNRHSEEHLEKIPRP 1242

  Fly  3464 IMGAPLQLAE---------------TIAQGGGEAPADATDAGLQEHSPEAAVDALTGGMSGGANT 3513
            :...|.:.:.               ||..|.|:.| :..|.| :..||:..       ...|.|:
Mouse  1243 LKSDPKEKSRDRSLSPRKGESKGRLTIKAGSGQDP-NRKDRG-RSSSPKKQ-------QKIGGNS 1298

  Fly  3514 LPPLSAGPLSAFQSLTGSLSMSGSLSSSALPQHKHSRM--SASAMSVMAATMTQQEEMLSHISHC 3576
            |           .:..|.||.:||..::..|:....::  ......|....:.|.:.
Mouse  1299 L-----------SNTEGKLSEAGSRRAAGHPRDSTEQLPDGREKSGVSRKDLKQSQP-------- 1344

  Fly  3577 HGLDDFGGLLGEPEAKSLVELLKLAVCGRCGPPSTSQTIADTLISLGAGTPAVAAMLLETCITEL 3641
                      |:...||              |...|..:.:|.:. ...|.:.|..:    ::|.
Mouse  1345 ----------GKTRTKS--------------PEKKSSKVDETSLP-SKKTSSTAGRV----VSEK 1380

  Fly  3642 EDLCTSRHCLGKLPKPVMQESSHPYVDNVNVTGVVRIPGAEMLRLEFDSQCSTEKRND 3699
            |.        ||  ||...|:|...|::..::       |:.|:.|...:.:....:|
Mouse  1381 EK--------GK--KPTAGETSRETVEHTQIS-------AKQLKQEAQEKTALGNADD 1421

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
HERC2	NP_608388.2	RCC1	638..683	CDD:278826
		RCC1	687..737	CDD:278826
		RCC1	740..787	CDD:278826
		RCC1	790..841	CDD:278826
		RCC1_2	828..857	CDD:290274
		Cyt-b5	1290..1363	CDD:278597
		MIB_HERC2	1928..1987	CDD:284184
		UBA_HERC2	2515..2559	CDD:270585	8/60 (13%)
		Cul7	2624..2699	CDD:288381	14/74 (19%)
		APC10-HERC2	2762..2932	CDD:176485	32/172 (19%)
		RCC1	3037..3088	CDD:278826	8/50 (16%)
		RCC1	3091..3140	CDD:278826	11/62 (18%)
		RCC1	3144..3194	CDD:278826	13/75 (17%)
		RCC1	3197..3246	CDD:278826	16/68 (24%)
		RCC1	3250..3298	CDD:278826	15/59 (25%)
		RCC1	3302..3350	CDD:278826	11/47 (23%)
		RCC1_2	4136..4166	CDD:290274
		RCC1	4154..4203	CDD:278826
		RCC1	4206..4257	CDD:278826
		RCC1	4260..4309	CDD:278826
		RCC1	4312..4360	CDD:278826
		RCC1	4364..4413	CDD:278826
		HECTc	4509..4880	CDD:238033
		HECTc	4553..4878	CDD:214523
Magi3	NP_001152826.1	Interaction with ADRB1 and TGFA. /evidence=ECO:0000269\|PubMed:15652357	18..108		26/111 (23%)
		PDZ_signaling	24..103	CDD:238492	22/99 (22%)
		NK	125..>199	CDD:302627	20/131 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	184..266		20/104 (19%)
		WW	297..329	CDD:197736	8/34 (24%)
		WW	345..375	CDD:238122	4/29 (14%)
		Interaction with PTEN. /evidence=ECO:0000250	413..495		22/118 (19%)
		PDZ	416..496	CDD:214570	21/116 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	551..575		3/23 (13%)
		PDZ	578..659	CDD:214570	16/85 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	664..691		7/37 (19%)
		PDZ	729..812	CDD:214570	17/87 (20%)
		Interaction with ADGRB1. /evidence=ECO:0000250	729..811		16/82 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	818..844		7/49 (14%)
		PDZ_signaling	851..936	CDD:238492	24/106 (23%)
		Interaction with LPAR2 and GRIN2B. /evidence=ECO:0000250	852..939		24/108 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	939..966		4/26 (15%)
		PDZ_signaling	1023..1101	CDD:238492	24/89 (27%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1109..1151		16/65 (25%)
		MIP-T3	<1166..>1431	CDD:287245	58/339 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1168..1476		58/337 (17%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900