Protein Alignment HERC2 and Ufd4

DIOPT Version :10

• Sequence 1: NP_608388.2
• Gene: HERC2 / 33035
• FlyBaseID: FBgn0031107
• Length: 4912
• Species: Drosophila melanogaster
• Sequence 2: NP_609369.1
• Gene: Ufd4 / 34378
• FlyBaseID: FBgn0032208
• Length: 2727
• Species: Drosophila melanogaster
• Alignment Length: 2756
• Identity: 519/2756 - (18%)
• Similarity: 850/2756 - (30%)
• Gaps: 1033/2756 - (37%)

  Fly   709 SLPTLVVALSDHMVVDVALGSGDAHSLA----------LTSE-GLVFAWGDGDYGKLGNGNCNGS 762
            |:...:.|:.:|:|| ..|.|..:..||          .|.| |.||   :|     |..||
  Fly    94 SIDGAIKAICNHLVV-ADLSSRTSRDLAEQCIKVLELICTREAGAVF---EG-----GGLNC--- 146

  Fly   763 LQPILVESLPRVQRVFAGSQ-----FSVALSSEGQLYTWGKATCLGHQLVERS---VQGCSVPRL 819
                 |.|..|    ..|||     ...|:|...:|.|          .||.:   :|.| |..|
  Fly   147 -----VLSFIR----DCGSQVHKDTLHSAMSVVSRLCT----------KVEPNTPCIQNC-VESL 191

  Fly   820 VSSLQHKRIVDVAVS--VAHCLALSSSGEVFGWGRNDSQQICPASVSSEPLLRTPILVSLPTFPA 882
            .:.|||:   |..||  ...|.|..:......|       :.||.: :|..|.|.:|..|.:.
  Fly   192 STLLQHE---DPMVSDGALKCFASVADRFTRKW-------VDPAPL-AEYGLTTELLKRLQSV-- 243

  Fly   883 SGIACTSAQSLVWTQSSHQGVPRRAPFVVDLGEPTFRLLDQLLGMCSSQDNRQTPNQE--SECIA 945
             |....|:.:...||                  ||           ||.....|.|.:  :|.:|
  Fly   244 -GGNTHSSLTAAGTQ------------------PT-----------SSSQPAATTNSDAINENVA 278

  Fly   946 VACLNLFRLQLLALIANGVEPRQVGLASGSRLLCSLKTRILGLAGGS-----QVLRT-IQVAAQQ 1004
            ..          |.|:|..:.:....|:..:.:.:..:.:..|..||     .:||: :..|.::
  Fly   279 GT----------ATISNSTKVKSSDAAASPQSISTTISLLSTLCRGSPSITHDILRSQLADALER 333

  Fly  1005 ALQDGWSVLLPTAAERAQTLTSLLPSEPGQASSAG-----------------------HRFMTDL 1046
            |||.....:|.  ..|...|..||..|..||.:.|                       ||.:.|.
  Fly   334 ALQGDERCVLD--CMRFADLLLLLLFEGRQALNRGSNNPNQGQLAPRPRRNNTNTDRTHRQLIDC 396

  Fly  1047 LVSSLMAEGGLESALQHAIRLESSSCSADCGDGVHLPL---------LQLITRLLSNNAALSQTR 1102
            :.|.      ...||:.||  ||.....:|.|.|...|         |:::..|....|.:::.:
  Fly   397 IRSK------DSEALREAI--ESGGIDVNCMDDVGQTLLNWASAFGTLEMVEYLCEKGADVNKGQ 453

  Fly  1103 LSPTL----------------------------GKQQ-EKEEEEREQQHQEPST---------SP 1129
            .|.:|                            ||.. :|..|..:..|:|.:.         ||
  Fly   454 RSSSLHYAACFGRPAIAKILLKFGAYPDLRDEDGKTPLDKARERLDDGHREVAAILQSPGEWMSP 518

  Fly  1130 SLSLLHRFQRLLLSHIHQAQPEEETTGAEALLLQYMHALIPACVATLQKAHELALQCREPGENSF 1194
            ..|||::..:.......:..||        :...|:..|:|             :.||    ...
  Fly   519 DHSLLNKDGKKYTLMEPRGDPE--------MAPIYLKVLLP-------------IFCR----TFL 558

  Fly  1195 GQMVGHLGRVLQADISDALLNELLVGLLLLKRDRPQCLGGLRWARLFLPLLRVLDRLNRALGEGE 1259
            |.|:|.:.|.     |.||:.:      :::...|..|..|                      .|
  Fly   559 GSMLGSVRRA-----SLALIKK------IVQYAYPTVLQSL----------------------SE 590

  Fly  1260 LRDGDDMGWPGIICRGGPKGGPPPADPETHYVRRADMENLLLDGSRCIILAGYVCDLSGYNCESE 1324
            ....:|..     ...|..||                 |||:                      |
  Fly   591 TSFSEDAA-----STSGQNGG-----------------NLLI----------------------E 611

  Fly  1325 TLRSVLDS---GLGKDLTAEMSSQVHRTAMEHILEHH-KLGKY-MVQA-----STEEKSSAPGPS 1379
            .:.||||:   |.|..:...:..::.....|..|:|. :||.: .|||     :.|.....||.
  Fly   612 VIASVLDNEDDGDGHLIVLNIIEEIMCKTQEEFLDHFARLGVFAKVQALMDTDAEELYVQLPGT- 675

  Fly  1380 RLTHFSSECALAQLLG---LVANLMCSGPALQPAE--LQ-----------CRQLDKSSLLSG--G 1426
                 ..|.|.||...   :||....|...::.|:  ||           ||..|...:.|.  .
  Fly   676 -----VEEPAAAQRSSTSVVVAPRPTSDDPMEDAKEILQGKPYHWREWSICRGRDCLYVWSDSVA 735

  Fly  1427 LQLLQPSNPF-----DEEKGEARSSHSCHSTAGNTPTELPPPLPMQQQLAPGRGKSQLQLRADAF 1486
            |:|...||.:     |.:.....||.|..:  ||..:|             .||:         |
  Fly   736 LELSNGSNGWFRFIIDGKLATMYSSGSPEN--GNDSSE-------------NRGE---------F 776

  Fly  1487 ISGLAEAR-------VSEP--PVAA--------WLALTERYCKAHNLMWHQEFATE-HPVQELER 1533
            :..|..||       ||:|  |.|:        |:..::   |.:.|..|.   || |.|..|:.
  Fly   777 LEKLMRARSCVIAGVVSQPILPTASALRLVVGNWVLQSQ---KTNQLQIHN---TEGHQVTVLQD 835

  Fly  1534 LLSAVLI------RHQYLGGLVL---------------NALETE--------------------- 1556
            .|...:.      :|.:....||               |..:||
  Fly   836 DLPGFIFESNRGTKHTFSAETVLGPDFASGWSTAKKKRNKSKTEGQKFQVRNLSREIYNKYFKSA 900

  Fly  1557 -VPPPRQLGEIIRLVHQAKWSVVRTRQQLNRSYKEVCAPILERLRFLLYE--VRPAISPQQRGL- 1617
             :.|...:..:..:|.|.:.|........|.:::......|.:|..|::|  |..|......||
  Fly   901 QIIPRGAVAILTDIVKQIELSFEEQHMAPNGNWETTLTDALMKLSQLIHEDGVVSAYEMHSSGLV 965

  Fly  1618 --------------------------RRLPILQR------------PPRFKMLVRRLLQELRSSR 1644
                                      :|:.:.::            .||.|.....|:|:|
  Fly   966 QALVAVLSVNHWETNSPRCKRNKMQKQRVSVFKKCILEDNVESATNKPRTKSTASILIQKL---- 1026

  Fly  1645 QPAKPEDLLNASIQQEQEKKP-------------QSMPKQ---ELEEQEEEETLL----RRLNER 1689
                      .|:.:..||.|             |.:.|:   .||..|.|.||.    |.|...
  Fly  1027 ----------VSVLESTEKLPVYLYDSPCTGYSLQILQKRLRFRLERAECESTLFDRSGRTLKME 1081

  Fly  1690 QIHGEGELDPALMQDIVDFALQDSCDVETARRAMYCQMQRYQLRLAGLQIVQQL-LELHGLL--- 1750
            .:...|:|...|::.:.    :...|::   |:.|..:::.:............ .:..|||
  Fly  1082 PLATIGQLSKYLLKMVA----KQWYDLD---RSTYFYLKKIREHRTATVFTHSFDFDEEGLLFYI 1139

  Fly  1751 ----------DAAQYSLLNGFLGLHLKSTSSGGGSTGSGSSLHVLGQ----LNMISAYQKARLLL 1801
                      :.|||.|        ::.|||.|.:...|....:|.:    ||..:...|.....
  Fly  1140 GSNAKTCDWVNPAQYGL--------VQVTSSEGKTLPYGKLEDILSRDSISLNCHTKDNKKAWFA 1196

  Fly  1802 AQSRV----LDWAVRELR-----RLVNQEQQGHARGKDSTNLGTYV----LLKRLPRARFLL--- 1850
            ....|    ..:.:|..|     .|.|...||...|...|.|.|:|    |::....|.:.:
  Fly  1197 IDLGVYIIPTAYTLRHARGYGRSALRNWLLQGSKDGSTWTTLSTHVDDKSLVEPGSTATWPINCA 1261

  Fly  1851 ---SVF--GLLAKELGPNELG---FLINSG--LLGTVLGLLAQTG-----GEGATGGQVHGELSI 1900
               ||:  .:..::.|.|..|   :|..||  :.|.|:|:....|     .|..|..:
  Fly  1262 TDDSVWYRHIRIQQNGRNASGQTHYLSLSGFEIYGRVVGVADDIGKSVKEAEAKTRRE------- 1319

  Fly  1901 LYEDSVLKQKSSKAQLSGPDLAKLMKIGTRIVRGADWKWGDQDGNPPGEGRIISEVGEDGWVRVE 1965
                    ::..:|||      |.|..|.|::||.||:|.:|||  ..||.|..|: .:||:.|:
  Fly  1320 --------RRQIRAQL------KHMTTGARVIRGVDWRWEEQDG--CAEGTITGEI-HNGWIDVK 1367

  Fly  1966 WYTGATNSYRMGKEGQYDLQLAD--------------SALNVASPTE--------------PERE 2002
            |..|..||||||.||:|||:|||              ||...|.|:|              |...
  Fly  1368 WDHGVRNSYRMGAEGKYDLKLADCEYLSAFDGNQSMGSASTAAKPSEKGGNTLTSRKSSSTPSLP 1432

  Fly  2003 DVSGSEASPTSDSHPSKLLRHCAAK------------------LLQILAVGTGLHGAQLDKDALR 2049
            :.:....:|...|:.:....:.|.|                  :...||:.|........|....
  Fly  1433 EATEKNQNPEGASNQTVSADNLAWKQTVETIAENVFASAKTQIISNQLAMNTSSSREARAKHKES 1497

  Fly  2050 GMTSMFR-TIIYPKPSMSNISYALGWLNLGFIRAISGDCPRL----CLELSTPGWLSHYLSLLEQ 2109
            |...|.: .|..|.|....:.:      :..:.||:...|.:    ..:|:|   :|..|||.|.
  Fly  1498 GTNQMHKDNISGPSPLSRELEH------ISDLSAINNSMPAINSSNVSDLAT---ISENLSLTEL 1553

  Fly  2110 PAGNEAGVYRQLHCLRLLQLILAQWGAEEEPRMPALVHQLFATLGRIALH------CPGDASLLP 2168
            ...|            :.:::...:...|.           .|..:.:.|      .|.:..:..
  Fly  1554 SKEN------------ICRVLTPSYKPAES-----------VTASQSSSHPDVQSSSPRENDIKN 1595

  Fly  2169 TAEGKARVLLTASHS-GSVAEELVALLRRLHTLPSWNPVINSFLAQKLCVAAELLAEQSHSTALD 2232
            .:..:....:.|::| ..::::|:|.| |...:....||                      |.|.
  Fly  1596 ISNIEENNKMNANNSVNKISKDLLANL-RTSNIAGCPPV----------------------TQLS 1637

  Fly  2233 SEQVFVLGVLGAMGGHDLRPRVGLHCFHEGSHMVIASFTPKGRCLLAP-GGVGSGVGFVKVQLPA 2296
            :|.:.::.          :.|.|:......|:.::::.|....|...| ||
  Fly  1638 TEALEMID----------KMRDGVDMIRNMSNSILSTDTFPVPCTNVPVGG-------------- 1678

  Fly  2297 VMPHLDHTVFSLSRLPMNEMLLNAWTVLLYGPAPE----------LRELPSSADGRLDLALLRAQ 2351
                        .:.|..:.|:|          |:          ..|.|:.:..:..:.|..||
  Fly  1679 ------------KKTPKAQALIN----------PDNANQKQIIVTSEEFPTKSSKKPSVTLKPAQ 1721

  Fly  2352 QLQMAVLHTNGVLYRHQVALRRILKQRAPGSIYASPDEPDRSDAESQQPGEQDQQLSSGSGQEP- 2415
            |                           |.::.:..|             .::|.:|:.:...|
  Fly  1722 Q---------------------------PNAVLSIVD-------------IKEQPISNENVSVPS 1746

  Fly  2416 QLLIQCILLRATQASPVKACYSYMDLATAALNCIQSLATQAHQELSEGGGVPPNGRALSSPPQPT 2480
            |:.|....|..|.||.|.   |..::||.. ..:::.|..|.:..|:|..:..|
  Fly  1747 QMSISVPNLTTTSASEVP---STSEVATHT-GLLETFAAIARRRTSQGTNIQDN----------- 1796

  Fly  2481 MVHGVPVYNVARKEQKPSEQVEQKSKWPAAATDAQLIGQIMEMGFTRRTVELALKQLSLQAEIMP 2545
                    .:...|...:|..:|.:.           |..:....| ..|:|||.. :..:.::.
  Fly  1797 --------QIMNAEANVNEHGDQNAS-----------GSFLGHSVT-SLVKLALSS-NFHSGLLS 1840

  Fly  2546 TPEQIVQWILEHPDVCANTIEEDTLPLASSASSHDPEADSDNECPSSNSTTSSSTSSDTVEGQPM 2610
            |.:       .:|.:.:|. .|:..|...|.:|...::.|......:.|.||:|:.|:.|..:..
  Fly  1841 TAQ-------SYPSLSSNN-SENIAPSNPSNTSAGQQSASTINHTLTMSLTSTSSDSEQVSLEDF 1897

  Fly  2611 AVSGPAPPV---------KFESRKDFQTADLYALYVRGLVRPGMTVRCCRDFEEIKQGDMGTVLI 2666
            ..|..||.:         ..|...:.:..|                    ::||:     |..|:
  Fly  1898 LESCRAPALLGDLDDEDDMDEDNDEEENED--------------------EYEEV-----GNTLL 1937

  Fly  2667 -----------VDTEGLHDLNVQV----DWRNHGSTYWVCFVHIELVEAAQTHHQPRPPPIAVGA 2716
                       :|.|.:.:..|.|    .|.:.       ||......|......|||
  Fly  1938 QVMVSRNLLTFMDDEAMENRLVGVTKRKSWDDE-------FVLKRQFSALIPAFDPRP------- 1988

  Fly  2717 RVRLRTSSLRYGMLCPLRLGRSQGSSAIGVVSSVRSKQLTVDFPDQPAWQGHINEVELVASQPTS 2781
                                   |.:.:...|.:....|..:.| :|...|....:|    ||..
  Fly  1989 -----------------------GRTNVNQTSDLEISPLGAELP-KPQQSGGPETIE----QPLL 2025

  Fly  2782 ATLPSLGDSCSQMPPSDL---IEDWS--RCIRSLTVSSNEAAAKHLLNGSNQPWQSCSSGPCRHW 2841
            . |...|.....:|..::   ..||:  |.::.|.                         .|...
  Fly  2026 G-LKLRGPGIGGIPEVEIDLSNTDWTIFRAVQELL-------------------------QCSQL 2064

  Fly  2842 IRLELHDRILVHSLTL---KVSPEDHSHMPSLLEIRVGDCVDSLKEYTWIP---VPAGASRVLLM 2900
            .:|:...:|...:.|:   :|||          |.:...|::| :|:...|   ..:|||  .|.
  Fly  2065 NKLDKFRKIWEPTYTIVYREVSP----------EAQESTCLES-EEFPQTPDVSSKSGAS--TLS 2116

  Fly  2901 QQVPTYYPW----------VEVVVKQCQNNGI-------QCKIHGIK------FVGRRQQPDLQH 2942
            ...|.:..:          .:|:....|.||:       ..|.||:.      |:.::....||.
  Fly  2117 PNSPMHIGFNVADNNLCSVDDVLELLTQINGLNQSEIDSDVKEHGVSVLSEDLFISKKITNKLQQ 2181

  Fly  2943 ILANAQFLAS-----------EYSAGVGPGST-----------AGAGAVSTSHEEAAAAPEQDLP 2985
            .:.:...|||           :....:.|..|           |....|....:.......|.:|
  Fly  2182 QIQDPLVLASNALPNWCENLNQSCPFLFPFETRQLYFNCTSFGASRSIVCLQSQRDVTVERQRIP 2246

  Fly  2986 CTVMVWGLNDKE-QLGGLKGSKVKVP 3010
              :|....:|.| ::|.||..:||||
  Fly  2247 --IMSPRRDDHEFRIGRLKHERVKVP 2270

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
HERC2	NP_608388.2	ATS1	625..>894	CDD:444065	52/205 (25%)
		Cyt-b5	1292..1364	CDD:459698	15/76 (20%)
		MIB_HERC2	1928..1988	CDD:461991	31/59 (53%)
		UBA_HERC2	2515..2559	CDD:270585	7/43 (16%)
		Cul7	2624..2699	CDD:463286	12/89 (13%)
		APC10-HERC2	2762..2932	CDD:176485	36/203 (18%)
		ATS1	2987..3320	CDD:444065	10/25 (40%)
		RCC1	3302..3350	CDD:395335
		ATS1	4050..4389	CDD:444065
		HECTc	4509..4880	CDD:238033
Ufd4	NP_609369.1	ANKYR	<375..>509	CDD:440430	25/141 (18%)
		ANK repeat	393..420	CDD:293786	9/34 (26%)
		ANK repeat	422..453	CDD:293786	5/30 (17%)
		ANK repeat	455..484	CDD:293786	2/28 (7%)
		Sad1_UNC	1173..1298	CDD:474037	27/124 (22%)
		MIB_HERC2	1333..1390	CDD:461991	31/59 (53%)
		HECTc	2265..2725	CDD:238033	4/6 (67%)