DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment pico and raph1b

DIOPT Version :9

Sequence 1:	NP_608363.1	Gene:	pico / 33003	FlyBaseID:	FBgn0261811	Length:	1162	Species:	Drosophila melanogaster
Sequence 2:	NP_001352514.1	Gene:	raph1b / 560319	ZFINID:	ZDB-GENE-090311-54	Length:	1565	Species:	Danio rerio

Alignment Length:	1241	Identity:	320/1241 - (25%)
Similarity:	482/1241 - (38%)	Gaps:	330/1241 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly    95 VIGAGAGAGVKLRRHSD---------RPLTPEAKQLSHTYRISMANLEDS----QESELDQILGE 146
            :.||..|...||.:..|         .||..|....:.:||.||.|:.::    :..:||.::.:
Zfish    27 MFGAWLGELDKLTQSLDDGRPEKVQKAPLRQETNLANFSYRFSMYNINEAINQGETVDLDALMAD 91

  Fly   147 LSLLEAQISYTEASMLPAMCAPSAGAQIAPPPGVTQLPGSA---PTMVSMSASSSRSHSRTNSTI 208
            |..:|.::|                 .|..|...| |.|.:   ||..|.||..|...|...||.
Zfish    92 LCSIEQELS-----------------TIGKPISKT-LDGKSKQRPTGRSASAKHSGGGSSGGSTS 138

  Fly   209 SADVSSCSSSGISENGHGLGLVLG--------------------------GPGSAGMMVQPPPPG 247
            |:..:|.:|:....|.....|...                          ...|:.....||...
Zfish   139 SSTRASPASTVRGGNSQSRPLASNFSLDDITAQLEKASLSMDEAAKQTSESSSSSYSSANPPSNS 203

  Fly   248 GMTMGITLGVVTPREPRTESPDNDSAFSDTVSLLSSESSASSNTSLQ-QQQQQQQQQHHQHQQHH 311
            ......::|.|:.:|.|:....:.|:.        :.:||||..||. :.|:.:.|..:|.|...
Zfish   204 HHRRTGSVGTVSDQEVRSIGHSSRSSV--------NSASASSMDSLDIRGQENETQSQNQSQTST 260

  Fly   312 QHQQQQQKPQLAGAEKLHHHGVHGQHGGQQSGANSI----------------------------T 348
            :|...:   :..|.....|.....|.|......:|.                            .
Zfish   261 EHVLLR---RANGKTTRRHLDFTSQEGEVDQSTHSYLDRETSLILKNIAGKPSHLLTKEEQAAKA 322

  Fly   349 KADKIQLALHKLESAPIRRLFVKAFTSDGASKSLLVDERMGCGHVTRLLADKNHVQMQSNWALVE 413
            ||:||::||.|::.|.:::|.::...||.:||:::||||.....:...|.||:|.....:|||||
Zfish   323 KAEKIRVALEKIKEAQVKKLVIRVHMSDESSKTMMVDERQTVRQLLDSLLDKSHCGYSPDWALVE 387

  Fly   414 HLGDLQMERLFEDHELLVDNLMTWHSDAGNRVLFQQRPDKVTLFLRPELYLPG----PQMAPGCQ 474
            .:.:|||||:|||||.||:||:.|..|:.|:::|.:|.:|..||..|:.||.|    .:||
Zfish   388 TIPELQMERIFEDHENLVENLLNWTRDSQNKLMFIERIEKYALFKNPQNYLLGRKETSEMA---- 448

  Fly   475 HDEQTRQMLLDEFF--DSHNQLQMDGPLYMKADPKKGWKRYHFVLRSSGLYYFPKEKTKNTRDLA 537
              ::.::.||:|.|  .|.:..:::|.|::|.|.||.||:.:|:||:||:|:.||.|.|.:|||.
Zfish   449 --DRNKEALLEECFCGSSVSVPEIEGVLWLKEDGKKSWKKRYFLLRASGIYFVPKGKAKASRDLV 511

  Fly   538 C-LNLFHGHNVYTGLGWRKKWKSPTDYTFGFKAVGDSSLGKSCRSLKMLCAEDLPTLDRWLTAIR 601
            | |.|.| .|||.|..:|.|:|:||||....|   ...:.|..:.:|.||.:|:.||.:|:..||
Zfish   512 CFLQLDH-VNVYYGQDYRSKYKAPTDYCLALK---HPQIQKKSQYIKYLCCDDVRTLHQWVNGIR 572

  Fly   602 VCKYGKQLWDSHKSLLEDLCLSRDDAVSQSSFAASMRSESISSISSAV---PSQCGSVSSAISSM 663
            :.||||||:.:::.     .:.|.:|....|   |:.|.||.|.:|:|   .||....|...|.|
Zfish   573 IAKYGKQLYVNYQE-----AMKRTEAAYDWS---SLSSTSIKSGTSSVSIPESQSNHSSQTDSGM 629

  Fly   664 SNSTSGRTSRASSSSSSGCLSDDNNAFDSEFTTGT-IKRKPSMKPNLPLTTMTRQLKEVGEITIC 727
            |:.||...:|:.|..||        .|...:..|| |:.....:|....:|.:........ .:.
Zfish   630 SDGTSSSHARSQSVVSS--------IFSEAWKRGTQIEESTKARPESIRSTHSSHSSHSSH-HVS 685

  Fly   728 ESAGGDASSPERSGTLTRRHSRRKSQESNGSGTLKRRPIAV-----------------PVATVVK 775
            :.....|..|.....|..|.|..::.....:.||.:.|..|                 |::::..
Zfish   686 QQQHLHAQQPHVHSILQSRGSVTQAPAQAPTQTLAQAPPQVAPAPPPPPPPPPPPPPPPISSIPH 750

  Fly   776 QTEP-MGSASST------SSSSNSTPTPTPSICAKPPPGDSASLMCSSTL-SLDSLPPPPPPPAL 832
            ...| |.:..||      :|.:.|...|.|.|..:.||....|...|:.: |..::|||||||
Zfish   751 HATPTMFAKYSTITRLQNASQATSHHKPQPQIQQQVPPVPPPSKPQSNNIPSGANIPPPPPPP-- 813

  Fly   833 DGSEDQDVYGSQLSLASLPPP----------PPPEDVLAMNYAEPSSPSTPTPMSTPMIMPNSNG 887
                         ..||:|||          ||...|:..  ..||.||.|.|...|.|||..:.
Zfish   814 -------------PPASMPPPGSAMAVLKLGPPAAAVIPQ--ISPSPPSPPPPPPPPPIMPVQSQ 863

  Fly   888 SLPPAVPAKPMK--------PAVKQAAGGLKAAPPYKA----PPDYVGP----ALLPGPP----- 931
            ..||..|..||:        |.....:..|...||.::    ||..:.|    ||.|.||
Zfish   864 PQPPPPPIMPMQSQPLPPPPPITPMQSHNLPPPPPMQSHALPPPPPITPMQSHALPPPPPITPMQ 928

  Fly   932 ---LPPPPPAQKKVSFADSPVLLRRKMCSPEPVLPQRS-----PSTTLSCHSSSSAGSAYQTYAP 988
               ||||||.....|.|..|         |.|:.|.:|     |...:...|......|......
Zfish   929 SHALPPPPPITPMQSHALPP---------PPPITPMQSHALPPPPPIMPMQSEPLPPPAQIMPMQ 984

  Fly   989 GPMLPPRADVARLSS---------LSNGSSSEVTSPKRLQESASNPPRDFLKD-LQRVMRKKWQV 1043
            ...|||...:.::.|         :|..|......|..||.:...||...:.: |::....|:
Zfish   985 SHPLPPPPPLVQMQSQPMPPPPPTVSFQSQPLPPPPPPLQANGLPPPPPLITNGLKQAFAHKF-- 1047

  Fly  1044 AQKCKAEPATTPHEVLGFRDFSNEDLLAAHNLNSGANSSHY------------------YRETAN 1090
                    .:||.:||                 ..||.:::                  |:...:
Zfish  1048 --------PSTPQDVL-----------------PPANQNNFPPPPPPPPPPPLAPTLPPYQHVTS 1087

  Fly  1091 VSHWVRKHYEYAHNALYENVHAQSAAAAGVPGSGEATTPPLPPPPGNSVAAKKRPPPPPPKRSDK 1155
            .|:                                   ||.||||         ||||||.   .
Zfish  1088 TSN-----------------------------------PPPPPPP---------PPPPPPA---P 1105

  Fly  1156 THLTNR 1161
            .||.|:
Zfish  1106 IHLANQ 1111

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
pico	NP_608363.1	GRB7_RA	364..451	CDD:176382	36/86 (42%)
		PH_APBB1IP	489..614	CDD:269961	54/125 (43%)
		PH	494..605	CDD:278594	47/111 (42%)
raph1b	NP_001352514.1	UBQ	340..421	CDD:320785	35/80 (44%)
raph1b	NP_001352514.1	PH_APBB1IP	463..585	CDD:269961	54/125 (43%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170593083
Domainoid	1	1.000	96	1.000	Domainoid score	I7261
eggNOG	1	0.900	-	-		E1_KOG3751
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	317	1.000	Inparanoid score	I2522
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0005410
OrthoInspector	1	1.000	-	-		otm25105
orthoMCL	1	0.900	-	-		OOG6_106466
Panther	1	1.100	-	-	O	PTHR11243
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2645
SonicParanoid	1	1.000	-	-		X3810
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	14	13.780