Protein Alignment nAChRalpha7 and chrna9a

DIOPT Version :10

• Sequence 1: NP_001162795.1
• Gene: nAChRalpha7 / 32928
• FlyBaseID: FBgn0086778
• Length: 564
• Species: Drosophila melanogaster
• Sequence 2: XP_021326868.1
• Gene: chrna9a / 568807
• ZFINID: ZDB-GENE-090312-63
• Length: 580
• Species: Danio rerio
• Alignment Length: 583
• Identity: 194/583 - (33%)
• Similarity: 304/583 - (52%)
• Gaps: 79/583 - (13%)

  Fly    42 LVYGLGLLIMIPACAAGPHEKRLLHALLDNYNSLERPVVNESDPLQLSFGLTLMQIIDVDEKNQL 106
            ||..:.||:.....|.|...::||:.|::.|::..|||.:....|.::..:||.||.|:||:||:
Zfish     6 LVIHISLLLHAVFSAQGRFAQKLLNDLMEGYSNALRPVEDTDKALNVTLQITLSQIKDMDERNQV 70

  Fly   107 LITNIWLKLEWNDMNLRWNSSEFGGVRDLRIPPHRLWKPDVLMYNSAD-EGFDGTYATNVVVRNN 170
            |.|.:|::..|:|..|.|:..|:.|:..:|||.:.:|:||:::||:|| |...|...||||:|.|
Zfish    71 LTTYLWVRQIWHDAYLSWDKEEYDGLEVIRIPSNLVWRPDIVLYNNADEEDSSGPPDTNVVLRYN 135

  Fly   171 GSCLYVPPGIFKSTCKIDITWFPFDDQRCEMKFGSWTYDGFQLDLQLQDEAGGDISSFITNGEWD 235
            |...:..|.|.||:||:|:::||||.|.|.:.||||||:|.|:|:.:..|: ||:|.|:.|.||:
Zfish   136 GEITWDSPAITKSSCKVDVSYFPFDSQECNLTFGSWTYNGNQVDIAMGMES-GDLSDFVDNVEWE 199

  Fly   236 LLGVPGKRNEIYYNCCPEPYIDITFAILIRRKTLYYFFNLIVPCVLIASMALLGFTLPPDSGEKL 300
            ..|:|..:|.|.|.||.:||.|||:.:|::|::.:|.|||::||.||:.:|.|||.||.|||||:
Zfish   200 CHGMPAVKNVIMYGCCSDPYPDITYTVLLKRRSSFYIFNLLLPCFLISFLAPLGFYLPADSGEKV 264

  Fly   301 SLGVTILLSLTVFLNMVAETMPATSDAVPLLGTYFNCIMFMVASSVVSTILILNYHHRNPDTHEM 365
            |||||:||:||||..||||:|| .|::|||:|.|:...|.|:.:|...||.|:|.|....:...:
Zfish   265 SLGVTVLLALTVFQLMVAESMP-PSESVPLIGKYYIATMTMITASTSLTIFIMNIHFCGAEAKPV 328

  Fly   366 SEWIRVIFLYWLPCILRMQRPGQVGYECPPPPS------SSSSSASGEK-----KQQIQNVELKE 419
            ..|.:|:.:.::..|..:.   :||..|..|.|      |....||.|:     |...::..|.|
Zfish   329 PHWAKVLIIDYMSKIFFVY---EVGENCTTPESDRGPFFSEDPLASLERDGYFDKGFYEDCHLDE 390

  Fly   420 RSSKSLLANVLDIDDDFRCN-----------HR-------CASATLP-----HQPTYYRTMYRQG 461
            |.....  |.....|....|           ||       .:|:..|     |.|.|...:.|.|
Zfish   391 RIRSQF--NGYSHRDHRHGNGYHHKNSSYRQHRHEQQRRTRSSSNSPVRQSSHHPKYTHFIGRDG 453

  Fly   462 D------------DGSVG-PVGPAGPVVD---------GRLHE--------AISHTCLTSS---- 492
            .            |..:. |....|...|         |.||:        |.|:...|:.
Zfish   454 SEKLPLTSQEKLKDSEISIPEKINGYTYDQGNGYLNGVGYLHDNGYSKTFGADSYNKTTTGTGCV 518

  Fly   493 -AEYELALILKELRWITEQLKKEDETSDITRDWKFAAMVVDRLCLIIFTLFTIIATLAVLFSA 554
             .:::  .:::.:.:|....:::........:||..|.|:||..:.:|.:...:.::.||..|
Zfish   519 CGKHQ--KLVRNIEYIANCFREQRGHQAKGAEWKKVAKVMDRFFMWVFFIMVFLMSILVLAKA 579

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha7	NP_001162795.1	LIC	59..548	CDD:455710	185/558 (33%)
chrna9a	XP_021326868.1	LGIC_ECD_nAChR_A9	25..232	CDD:349823	89/207 (43%)
		Cys-loop	150..164	CDD:349823	8/13 (62%)
		LGIC_TM_cation	233..>320	CDD:349853	49/87 (56%)
		TM1 helix	233..257	CDD:349853	12/23 (52%)
		TM2 helix	265..286	CDD:349853	15/20 (75%)
		TM3 helix	296..318	CDD:349853	7/21 (33%)