DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment HisRS and EIF2AK4

DIOPT Version :9

Sequence 1:	NP_573305.2	Gene:	HisRS / 32841	FlyBaseID:	FBgn0027087	Length:	564	Species:	Drosophila melanogaster
Sequence 2:	NP_001013725.2	Gene:	EIF2AK4 / 440275	HGNCID:	19687	Length:	1649	Species:	Homo sapiens

Alignment Length:	590	Identity:	140/590 - (23%)
Similarity:	242/590 - (41%)	Gaps:	143/590 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly    46 PAQRQQQQQ--------PQKQQQAQVQQQQHEQLHQIDEEVARLL-----------ALKATLGGD 91
            ||:|....:        |.:.:::::.:..|..|..:|.:..|.:           |:..|...|
Human   986 PAKRPTATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSD 1050

  Fly    92 AAPTNQKFTLKTPKGTRDYGPQQMTLRQGVLDKIVQVFKRHGGEAIDTPVFELKEVLTGKYGEDS 156
            ....|  |:::|.|           ::|.|.:.|:::|||||...:.||:...:          :
Human  1051 ILKGN--FSIRTAK-----------MQQHVCETIIRIFKRHGAVQLCTPLLLPR----------N 1092

  Fly   157 KLIYD------LKDQGGEILSMRYDLTVPLARYLAMNKISSIKRYHIAKVYR-----RDNPAMTK 210
            :.||:      ..|..|.::.:.:||.:|.|||:|.|.|.::|||.|.:|:|     |.:|
Human  1093 RQIYEHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHP---- 1153

  Fly   211 GRYREFYQCDFDI-AGTYDPMLPDAECVKIVSEILD---TLDIGDYVIKLNHRQLLDGMFQACGV 271
               :|..:|.||| ..|.:..||.||.:..:.||:.   .|...:|.|.|||..||..:...||:
Human  1154 ---KELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGI 1215

  Fly   272 PADSFRTIC-----SAVDKLDKSPWADVRKEMVDEK----GLDEAAADRIGEYVRLSGGAELVEQ 327
            |.|....:.     :..:||       .|:| |:.|    .|...:..|:.:::...|..:
Human  1216 PEDKLSQVYIILYDAVTEKL-------TRRE-VEAKFCNLSLSSNSLCRLYKFIEQKGDLQ---- 1268

  Fly   328 LLANEKLKAVPNAVKGLEGMKQLLKYC-----SIFGLDKRVSFDLSLARGLDYYTGVIYEGVLKG 387
                :.:..:.:.:|...|:.||:||.     .:.||.|::...|.:...|    |::|
Human  1269 ----DLMPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLGIKLQVLINL----GLVY------ 1319

  Fly   388 ESATVASPAKTSQQNG--------EQANEPATVGSVAGGGRYDNLVGMF-DPR--GKAVPCVGVS 441
                     |..|.||        .:..:.|....:|.|||||.|:..| .|:  |.....:|||
Human  1320 ---------KVQQHNGIIFQFVAFIKRRQRAVPEILAAGGRYDLLIPQFRGPQALGPVPTAIGVS 1375

  Fly   442 IGVERIFSVLEARAAASGLK--LRTSDVEVYVASAHKGLHEQRLKVLNLLWDAGVKAEHSYKLNP 504
            |.:::|      .||...::  :..|..::.|.|..:....:.:.:...||.||:.||..|..:.
Human  1376 IAIDKI------SAAVLNMEESVTISSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQ 1434

  Fly   505 KLLVQLQHCEEHQIPLVVVLGDAELAQGLVKLREVTTREETNV-----------KLEDLAAEIRR 558
            ......::|..|:|..|.::.|.|.:...||..|...:.|..|           ||.....:.|.
Human  1435 SQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLETELVDHVLQKLRTKVTDERN 1499

  Fly   559 RQQAS 563
            .::||
Human  1500 GREAS 1504

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
HisRS	NP_573305.2	S15_NS1_EPRS_RNA-bind	6..92	CDD:294248	10/64 (16%)
		HisS	98..561	CDD:223202	126/515 (24%)
		HisRS-like_core	114..452	CDD:238396	97/377 (26%)
		HisRS_anticodon	467..557	CDD:238436	22/100 (22%)
EIF2AK4	NP_001013725.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..25
		RWD	18..134	CDD:283440
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	138..158
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	227..256
		Pkinase	296..539	CDD:278497
		PK_eIF2AK_GCN2_rpt1	299..538	CDD:270914
		STKc_EIF2AK4_GCN2_rpt2	583..999	CDD:270948	3/12 (25%)
		S_TKc	590..1001	CDD:214567	3/14 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	660..750
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	765..788
		Histidyl-tRNA synthetase-like	1022..1493		131/541 (24%)
		class_II_aaRS-like_core	1061..1385	CDD:294192	99/392 (25%)
		HisS	1065..1490	CDD:223202	120/482 (25%)
		HGTP_anticodon	1395..1641	CDD:294191	26/110 (24%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0124
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.810