Protein Alignment Sh and Kcnd2

DIOPT Version :10

• Sequence 1: NP_728123.1
• Gene: Sh / 32780
• FlyBaseID: FBgn0003380
• Length: 655
• Species: Drosophila melanogaster
• Sequence 2: NP_113918.2
• Gene: Kcnd2 / 65180
• RGDID: 68393
• Length: 630
• Species: Rattus norvegicus
• Alignment Length: 587
• Identity: 185/587 - (31%)
• Similarity: 290/587 - (49%)
• Gaps: 125/587 - (21%)

  Fly    99 VVINVSGLRFETQLRTLNQFPDTLLGDPARRLRYFDPLRNEYFFDRSRPSFDAILYYYQSGGRLR 163
            :|:||||.||:|...||.::||||||...|.. ::.|...:|||||....|..||.:|:: |:|.
  Rat    43 IVLNVSGTRFQTWQDTLERYPDTLLGSSERDF-FYHPETQQYFFDRDPDIFRHILNFYRT-GKLH 105

  Fly   164 RPVNVPLDVFSEEIKFYEL-----GDQAINKF----REDEGFIKEE-------ERPLPDNEKQRK 212
            .|.:..:..:.||:.|:.|     ||....::    ||:...::::       |..||....:::
  Rat   106 YPRHECISAYDEELAFFGLIPEIIGDCCYEEYKDRRRENAERLQDDADTDNTGESALPTMTARQR 170

  Fly   213 VWLLFEYPESSQAARVVAIISVFVILLSIVIFCLETLP---EFKHYKVFNTTTNGTKIEEDEVP- 273
            ||..||.|.:|..|.|...::.|.|.:|::...:||:|   ...|.|              |:|
  Rat   171 VWRAFENPHTSTMALVFYYVTGFFIAVSVIANVVETVPCGSSPGHIK--------------ELPC 221

  Fly   274 --DITDPFFLIETLCIIWFTFELTVRFLACPNKLNFCRDVMNVIDIIAIIPYFITLATVVAEEED 336
              .....||.::|.|::.||.|..:|..|.|::..|.|.||::||::||:||:|.|  |:.:.||
  Rat   222 GERYAVAFFCLDTACVMIFTVEYLLRLAAAPSRYRFVRSVMSIIDVVAILPYYIGL--VMTDNED 284

  Fly   337 TLNLPKAPVSPQDKSSNQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGRTLKASMRELGLLIF 401
                               :|.|.: .:|:.|||||||.||||:||:|||.|||:...|||.|:|
  Rat   285 -------------------VSGAFV-TLRVFRVFRIFKFSRHSQGLRILGYTLKSCASELGFLLF 329

  Fly   402 FLFIGVVLFSSAVYFAEAGSENSFFKSIPDAFWWAVVTMTTVGYGDMTPVGVWGKIVGSLCAIAG 466
            .|.:.:::|::.:::||.||..|.|.|||.|||:.:|||||:|||||.|..:.|||.||:|:::|
  Rat   330 SLTMAIIIFATVMFYAEKGSSASKFTSIPAAFWYTIVTMTTLGYGDMVPKTIAGKIFGSICSLSG 394

  Fly   467 VLTIALPVPVIVSNFNYFYHRETDQEEMQSQNFNHVTSCPYLPGTLGQHMKKSSLS--------E 523
            ||.||||||||||||:..||:....::.::|                   ||:.|:        .
  Rat   395 VLVIALPVPVIVSNFSRIYHQNQRADKRRAQ-------------------KKARLARIRAAKSGS 440

  Fly   524 SSSDMMDLDDGVESTPGLTETHPGRSAVAPFLGAQQQQQQPV-ASSLSMSIDKQLQHPLQQLTQT 587
            :::.|....:|:.|..                 .|..:.:|. .|....|.:.|..|.|..|.:|
  Rat   441 ANAYMQSKRNGLLSNQ-----------------LQSSEDEPAFVSKSGSSFETQHHHLLHCLEKT 488

  Fly   588 QLYQQQQQQQQQQQNGFKQQQQQTQQQLQQQQSHTINASAAAATSGSGSSGLT-----MRHNNAL 647
            ..::...:|.      |::...:..         |:|..::.:.|.|...|:|     .||....
  Rat   489 TNHEFVDEQV------FEESCMEVA---------TVNRPSSHSPSLSSQQGVTSTCCSRRHKKTF 538

  Fly   648 AV 649
            .:
  Rat   539 RI 540

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Sh	NP_728123.1	BTB_POZ	97..223	CDD:453885	46/139 (33%)
		Ion_trans	226..488	CDD:459842	112/267 (42%)
Kcnd2	NP_113918.2	Interaction with KCNIP1, KCNIP2, and other family members. /evidence=ECO:0000305|PubMed:14980206, ECO:0000305|PubMed:14980207	2..20
		BTB_POZ	6..144	CDD:453885	37/102 (36%)
		Interaction with KCNIP1. /evidence=ECO:0000305|PubMed:14980207	71..90		7/19 (37%)
		Ion_trans	184..415	CDD:459842	111/266 (42%)
		S4-S5 linker. /evidence=ECO:0000250|UniProtKB:P63142	308..321		8/12 (67%)
		Selectivity filter. /evidence=ECO:0000250|UniProtKB:P63142	370..375		3/4 (75%)
		DUF3399	470..546	CDD:463381	16/86 (19%)
		Important for normal channel activation and inactivation, for interaction with KCNIP2, and probably other family members as well. /evidence=ECO:0000305|PubMed:16820361	474..630		15/82 (18%)
		Required for dendritic targeting. /evidence=ECO:0000269|PubMed:12592409	474..489		4/14 (29%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	600..623
		PDZ-binding. /evidence=ECO:0000269|PubMed:11923279, ECO:0000269|PubMed:14559911	627..630