DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Sh and Kcnd2

DIOPT Version :9

Sequence 1:	NP_001303569.1	Gene:	Sh / 32780	FlyBaseID:	FBgn0003380	Length:	655	Species:	Drosophila melanogaster
Sequence 2:	NP_062671.1	Gene:	Kcnd2 / 16508	MGIID:	102663	Length:	630	Species:	Mus musculus

Alignment Length:	587	Identity:	185/587 - (31%)
Similarity:	291/587 - (49%)	Gaps:	125/587 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly    99 VVINVSGLRFETQLRTLNQFPDTLLGDPARRLRYFDPLRNEYFFDRSRPSFDAILYYYQSGGRLR 163
            :|:||||.||:|...||.::||||||...|.. ::.|...:|||||....|..||.:|:: |:|.
Mouse    43 IVLNVSGTRFQTWQDTLERYPDTLLGSSERDF-FYHPETQQYFFDRDPDIFRHILNFYRT-GKLH 105

  Fly   164 RPVNVPLDVFSEEIKFYEL-----GDQAINKF----REDEGFIKEE-------ERPLPDNEKQRK 212
            .|.:..:..:.||:.|:.|     ||....::    ||:...::::       |..||....:::
Mouse   106 YPRHECISAYDEELAFFGLIPEIIGDCCYEEYKDRRRENAERLQDDADTDNTGESALPTMTARQR 170

  Fly   213 VWLLFEYPESSQAARVVAIISVFVILLSIVIFCLETLP---EFKHYKVFNTTTNGTKIEEDEVP- 273
            ||..||.|.:|..|.|...::.|.|.:|::...:||:|   ...|.|              |:|
Mouse   171 VWRAFENPHTSTMALVFYYVTGFFIAVSVIANVVETVPCGSSPGHIK--------------ELPC 221

  Fly   274 --DITDPFFLIETLCIIWFTFELTVRFLACPNKLNFCRDVMNVIDIIAIIPYFITLATVVAEEED 336
              .....||.::|.|::.||.|..:|..|.|::..|.|.||::||::||:||:|.|  |:.:.||
Mouse   222 GERYAVAFFCLDTACVMIFTVEYLLRLAAAPSRYRFVRSVMSIIDVVAILPYYIGL--VMTDNED 284

  Fly   337 TLNLPKAPVSPQDKSSNQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGRTLKASMRELGLLIF 401
                               :|.|.: .:|:.|||||||.||||:||:|||.|||:...|||.|:|
Mouse   285 -------------------VSGAFV-TLRVFRVFRIFKFSRHSQGLRILGYTLKSCASELGFLLF 329

  Fly   402 FLFIGVVLFSSAVYFAEAGSDSSFFKSIPDGFWWAVVTMTTVGYGDMRPVGVWGKIVGSLCAIAG 466
            .|.:.:::|::.:::||.||.:|.|.|||..||:.:|||||:|||||.|..:.|||.||:|:::|
Mouse   330 SLTMAIIIFATVMFYAEKGSSASKFTSIPAAFWYTIVTMTTLGYGDMVPKTIAGKIFGSICSLSG 394

  Fly   467 VLTIALPVPVIVSNFNYFYHRETDQEEMQSQNFNHVTSCPYLPGTLGQHMKKSSLS--------E 523
            ||.||||||||||||:..||:....::.::|                   ||:.|:        .
Mouse   395 VLVIALPVPVIVSNFSRIYHQNQRADKRRAQ-------------------KKARLARIRAAKSGS 440

  Fly   524 SSSDMMDLDDGVESTPGLTETHPGRSAVAPFLGAQQQQQQPVASSLS-MSIDKQLQHPLQQLTQT 587
            :::.|....:|:.|..                 .|..:.:|...|.| .|.:.|..|.|..|.:|
Mouse   441 ANAYMQSKRNGLLSNQ-----------------LQSSEDEPAFISKSGSSFETQHHHLLHCLEKT 488

  Fly   588 QLYQQQQQQQQQQQNGFKQQQQQTQQQLQQQQSHTINASAAAATSGSGSSGLT-----MRHNNAL 647
            ..::...:|.      |::...:..         |:|..::.:.|.|...|:|     .||....
Mouse   489 TNHEFVDEQV------FEESCMEVA---------TVNRPSSHSPSLSSQQGVTSTCCSRRHKKTF 538

  Fly   648 AV 649
            .:
Mouse   539 RI 540

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Sh	NP_001303569.1	BTB_2	99..187	CDD:280393	36/92 (39%)
		BTB	99..182	CDD:197585	33/82 (40%)
		Ion_trans	274..488	CDD:278921	99/213 (46%)
		Ion_trans_2	403..479	CDD:285168	41/75 (55%)
Kcnd2	NP_062671.1	Interaction with KCNIP1, KCNIP2, and other family members. /evidence=ECO:0000250\|UniProtKB:Q63881	2..20
		Shal-type	3..28	CDD:288455
		BTB	43..140	CDD:197585	36/98 (37%)
		BTB_2	43..132	CDD:280393	35/90 (39%)
		Interaction with KCNIP1. /evidence=ECO:0000250\|UniProtKB:Q63881	71..90		7/19 (37%)
		Ion_trans	216..415	CDD:278921	101/234 (43%)
		S4-S5 linker. /evidence=ECO:0000250\|UniProtKB:P63142	308..321		8/12 (67%)
		Ion_trans_2	331..411	CDD:285168	45/79 (57%)
		Selectivity filter. /evidence=ECO:0000250\|UniProtKB:P63142	370..375		3/4 (75%)
		DUF3399	445..546	CDD:288712	23/128 (18%)
		Important for normal channel activation and inactivation, for interaction with KCNIP2, and probably other family members as well. /evidence=ECO:0000250\|UniProtKB:Q63881	474..630		15/82 (18%)
		Required for dendritic targeting. /evidence=ECO:0000250\|UniProtKB:Q63881	474..489		4/14 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	600..623
		PDZ-binding. /evidence=ECO:0000250\|UniProtKB:Q63881	627..630
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG2126
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X19
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.810