DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Sh and Kcnb1

DIOPT Version :9

Sequence 1:	NP_001303569.1	Gene:	Sh / 32780	FlyBaseID:	FBgn0003380	Length:	655	Species:	Drosophila melanogaster
Sequence 2:	NP_032446.2	Gene:	Kcnb1 / 16500	MGIID:	96666	Length:	857	Species:	Mus musculus

Alignment Length:	618	Identity:	204/618 - (33%)
Similarity:	305/618 - (49%)	Gaps:	113/618 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly    98 RVVINVSGLRFETQLRTLNQFPDTLLGDPARRLRYFDPL----------RNEYFFDRSRPSFDAI 152
            ||.:||.||..|...|||::.|.|.|| ..|.....|.|          .|||||||...:|.:|
Mouse    32 RVRLNVGGLAHEVLWRTLDRLPRTRLG-KLRDCNTHDSLLQVCDDYSLEDNEYFFDRHPGAFTSI 95

  Fly   153 LYYYQSGGRLRRPVNVPLDVFSEEI----------------KFYELGDQAINKFREDEGFIKEEE 201
            |.:|:: |||.....:....||:|:                ::::..:|...:.:.:...::|.|
Mouse    96 LNFYRT-GRLHMMEEMCALSFSQELDYWGIDEIYLESCCQARYHQKKEQMNEELKREAETLRERE 159

  Fly   202 RPLPDN----EKQRKVWLLFEYPESSQAARVVAIISVFVILLSIVIFCLETLPEFKHYKVFNTTT 262
            ....||    ||::|:|.|.|.|.||.||:::||||:..|:||.:...|.||||.:....|..:|
Mouse   160 GEEFDNTCCAEKRKKLWDLLEKPNSSVAAKILAIISIMFIVLSTIALSLNTLPELQSLDEFGQST 224

  Fly   263 NGTKIEEDEVPDITDPFFLIETLCIIWFTFELTVRFLACPNKLNFCRDVMNVIDIIAIIPYFITL 327
            :..::..            :|.:||.|||.|..:|||:.|.|..|.:..:|.||::||:||::|:
Mouse   225 DNPQLAH------------VEAVCIAWFTMEYLLRFLSSPKKWKFFKGPLNAIDLLAILPYYVTI 277

  Fly   328 ATVVAEEEDTLNLPKAPVSPQDKSSNQAMSL-AILRVIRLVRVFRIFKLSRHSKGLQILGRTLKA 391
            .                ::..:||..|..:: .::::.|::|:.||.||:|||.|||.||.||:.
Mouse   278 F----------------LTESNKSVLQFQNVRRVVQIFRIMRILRILKLARHSTGLQSLGFTLRR 326

  Fly   392 SMRELGLLIFFLFIGVVLFSSAVYFAEAGSDSSFFKSIPDGFWWAVVTMTTVGYGDMRPVGVWGK 456
            |..||||||.||.:|:::|||.|:|||...|.:.|||||..||||.:|||||||||:.|..:.||
Mouse   327 SYNELGLLILFLAMGIMIFSSLVFFAEKDEDDTKFKSIPASFWWATITMTTVGYGDIYPKTLLGK 391

  Fly   457 IVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETDQE---------EMQSQNFNHVTSCPYLPGTL 512
            |||.||.|||||.||||:|:||:||:.||..:..||         |...:|.:.|:
Mouse   392 IVGGLCCIAGVLVIALPIPIIVNNFSEFYKEQKRQEKAIKRREALERAKRNGSIVS--------- 447

  Fly   513 GQHMKKSSLSESSSDMMDL-----DDGVESTPGLTETH--PGR-----------SAVAPFLGAQQ 559
               |........|.:|||:     .:||.....:.:.|  |.:           |:...|...:|
Mouse   448 ---MNMKDAFARSIEMMDIVVEKNGEGVAKKDKVQDNHLSPNKWKWTKRALSETSSSKSFETKEQ 509

  Fly   560 QQQQPVASSLSMSIDKQLQH-PLQQLTQTQLYQQQQQQQQQQQNGFKQQQQQTQQQLQQQQSHTI 623
            ...:...||.|.      || .:|||  ..:|.:..:.|.|.....|:...|:|.|.:.:.....
Mouse   510 GSPEKARSSSSP------QHLNVQQL--QDMYSKMAKTQSQPILNTKEMAPQSQPQEELEMGSMP 566

  Fly   624 NASAAAATSGSGSSGLTMRHNNAL--AVSIETD 654
            :..|...|...|.  :.||..:::  .:|..||
Mouse   567 SPVAPLPTRTEGV--IDMRSMSSIDSFISCATD 597

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Sh	NP_001303569.1	BTB_2	99..187	CDD:280393	34/113 (30%)
		BTB	99..182	CDD:197585	33/108 (31%)
		Ion_trans	274..488	CDD:278921	100/214 (47%)
		Ion_trans_2	403..479	CDD:285168	47/75 (63%)
Kcnb1	NP_032446.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..21
		BTB_POZ_KCNB2	15..141	CDD:349719	34/110 (31%)
		Self-association. /evidence=ECO:0000250\|UniProtKB:P15387	59..75		3/15 (20%)
		Ion_trans	188..424	CDD:366146	115/263 (44%)
		Selectivity filter. /evidence=ECO:0000250\|UniProtKB:P63142	377..382		4/4 (100%)
		Self-association. /evidence=ECO:0000250\|UniProtKB:P15387	448..481		7/32 (22%)
		Kv2channel	467..678	CDD:367541	30/141 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	475..569		20/101 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	608..627
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	768..802
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	816..857
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG2126
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X19
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.810