DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Sh and Kcna2

DIOPT Version :9

Sequence 1:	NP_001303569.1	Gene:	Sh / 32780	FlyBaseID:	FBgn0003380	Length:	655	Species:	Drosophila melanogaster
Sequence 2:	NP_032443.3	Gene:	Kcna2 / 16490	MGIID:	96659	Length:	499	Species:	Mus musculus

Alignment Length:	467	Identity:	319/467 - (68%)
Similarity:	371/467 - (79%)	Gaps:	24/467 - (5%)

- Green bases have known domain annotations that are detailed below.

  Fly    81 GGPQHFEPIPHDHDFCERVVINVSGLRFETQLRTLNQFPDTLLGDPARRLRYFDPLRNEYFFDRS 145
            |.||.......||:.|||||||:|||||||||:||.|||:||||||.:|:||||||||||||||:
Mouse    17 GHPQDTYDPEADHECCERVVINISGLRFETQLKTLAQFPETLLGDPKKRMRYFDPLRNEYFFDRN 81

  Fly   146 RPSFDAILYYYQSGGRLRRPVNVPLDVFSEEIKFYELGDQAINKFREDEGFIKEEERPLPDNEKQ 210
            ||||||||||||||||||||||||||:|||||:|||||::|:..||||||:|||||||||:||.|
Mouse    82 RPSFDAILYYYQSGGRLRRPVNVPLDIFSEEIRFYELGEEAMEMFREDEGYIKEEERPLPENEFQ 146

  Fly   211 RKVWLLFEYPESSQAARVVAIISVFVILLSIVIFCLETLPEFKHYK--------VFNTTTNGTKI 267
            |:|||||||||||..||::||:||.|||:|||.|||||||.|:...        .|:|.:|.| |
Mouse   147 RQVWLLFEYPESSGPARIIAIVSVMVILISIVSFCLETLPIFRDENEDMHGGGVTFHTYSNST-I 210

  Fly   268 EEDEVPDITDPFFLIETLCIIWFTFELTVRFLACPNKLNFCRDVMNVIDIIAIIPYFITLATVVA 332
            ...:....|||||::||||||||:||..|||.|||:|..|..::||:|||:|||||||||.|.:|
Mouse   211 GYQQSTSFTDPFFIVETLCIIWFSFEFLVRFFACPSKAGFFTNIMNIIDIVAIIPYFITLGTELA 275

  Fly   333 EEEDTLNLPKAPVSPQD-KSSNQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGRTLKASMREL 396
            |:            |:| :...|||||||||||||||||||||||||||||||||:|||||||||
Mouse   276 EK------------PEDAQQGQQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLKASMREL 328

  Fly   397 GLLIFFLFIGVVLFSSAVYFAEAGSDSSFFKSIPDGFWWAVVTMTTVGYGDMRPVGVWGKIVGSL 461
            |||||||||||:|||||||||||....|.|.||||.||||||:|||||||||.|..:.|||||||
Mouse   329 GLLIFFLFIGVILFSSAVYFAEADERDSQFPSIPDAFWWAVVSMTTVGYGDMVPTTIGGKIVGSL 393

  Fly   462 CAIAGVLTIALPVPVIVSNFNYFYHRETDQEEMQSQNFNHVTSCPYLPGTLGQHMKKSSLSESSS 526
            ||||||||||||||||||||||||||||:.|| |:| :..|||||.:|.:......:|:.:.|.|
Mouse   394 CAIAGVLTIALPVPVIVSNFNYFYHRETEGEE-QAQ-YLQVTSCPKIPSSPDLKKSRSASTISKS 456

  Fly   527 DMMDLDDGVEST 538
            |.|::.:||.::
Mouse   457 DYMEIQEGVNNS 468

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Sh	NP_001303569.1	BTB_2	99..187	CDD:280393	75/87 (86%)
		BTB	99..182	CDD:197585	72/82 (88%)
		Ion_trans	274..488	CDD:278921	160/214 (75%)
		Ion_trans_2	403..479	CDD:285168	61/75 (81%)
Kcna2	NP_032443.3	Tetramerization domain. /evidence=ECO:0000250\|UniProtKB:P63142	1..125		84/107 (79%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..26		3/8 (38%)
		BTB_POZ	33..159	CDD:365784	106/125 (85%)
		Ion_trans	162..420	CDD:395416	185/270 (69%)
		S4-S5 linker. /evidence=ECO:0000250\|UniProtKB:P63142	312..325		11/12 (92%)
		Selectivity filter. /evidence=ECO:0000250\|UniProtKB:P63142	374..379		4/4 (100%)
		PDZ-binding. /evidence=ECO:0000250\|UniProtKB:P63142	497..499
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	361	1.000	Domainoid score	I948
eggNOG	1	0.900	-	-		E1_COG2126
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	620	1.000	Inparanoid score	I878
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG48093
OrthoDB	1	1.010	-	-		D307298at33208
OrthoFinder	1	1.000	-	-		FOG0000074
OrthoInspector	1	1.000	-	-		otm44254
orthoMCL	1	0.900	-	-		OOG6_101149
Panther	1	1.100	-	-	O	PTHR11537
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3654
SonicParanoid	1	1.000	-	-		X19
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	13	12.910