DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment mnb and Hipk2

DIOPT Version :9

Sequence 1:	NP_728107.2	Gene:	mnb / 32771	FlyBaseID:	FBgn0259168	Length:	1047	Species:	Drosophila melanogaster
Sequence 2:	NP_034563.2	Gene:	Hipk2 / 15258	MGIID:	1314872	Length:	1196	Species:	Mus musculus

Alignment Length:	1120	Identity:	266/1120 - (23%)
Similarity:	410/1120 - (36%)	Gaps:	338/1120 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly   145 SSSRMKAERALYYTMSAYGSSGGSVDAAQGSGSGGGRQRHAPLYGRFVD-----------AEDLP 198
            |..::|.|.:..:.|:.|||                   |:.:|.:..:           :..||
Mouse    27 SVKKLKVEPSSNWDMTGYGS-------------------HSKVYSQSKNIPPSQPASTTVSTSLP 72

  Fly   199 ATHRDVMHHHS--SPSSSSEVRAMQARIPNHFREPASGP---LRKLSVDLIKTYKHINEVYYAKK 258
            ..:..:.:..:  .|.|:..:....|...:...:...||   :|:.:|.|:.||:...      .
Mouse    73 IPNPSLPYEQTIIFPGSTGHIVVTSASSTSVTGQVLGGPHNLMRRSTVSLLDTYQKCG------L 131

  Fly   259 KRRAQQTQGDDD---------------------SSNKKERKLYNDGYDDDNHDYIIKNGE---KF 299
            ||::::.:....                     ::........|.|.:.:. ||.:...|   ..
Mouse   132 KRKSEEIENTSSVQIIEEHPPMIQNNASGATVATATTSTATSKNSGSNSEG-DYQLVQHEVLCSM 195

  Fly   300 LDRYEIDSLIGKGSFGQVVKAYDHEEQCHVAIKIIKNKKPFLNQAQIEVKLLEMMNRADAENKYY 364
            .:.||:...:|:|:||||||.:.......|||||:||...:..|.||||.:|..::...|:: |.
Mouse   196 TNTYEVLEFLGRGTFGQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARLSTESADD-YN 259

  Fly   365 IVKLKRHFMWRNHLCLVFELLSYNLYDLLRNTNFRGVSLNLTRKFAQQLCTALLFLSTPELNIIH 429
            .|:....|..:||.|||||:|..||||.|:...|..:.|...|...||:.|||:.|.:  |.:||
Mouse   260 FVRAYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPVLQQVATALMKLKS--LGLIH 322

  Fly   430 CDLKPENILLCNPKRS--AIKIVDFGSSCQLGQRIYH-YIQSRFYRSPEVLLGIQYDLAIDMWSL 491
            .|||||||:|.:|.|.  .:|::||||:..:.:.:.. |:|||:||:||::||:.:..|||||||
Mouse   323 ADLKPENIMLVDPSRQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSL 387

  Fly   492 GCILVEMHTGEPLFSGCNEVDQMNKIVEVLGMPPKYLLDQAHKTRKFFDKIVADGSYVL------ 550
            ||::.|:..|.||:.|.:|.||:..|.:..|:|.:|||....||.:||:: ..|..|.|
Mouse   388 GCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFNR-DTDSPYPLWRLKTP 451

  Fly   551 --------KKNQNGRKYKPPGSRKLHDI--LGVETGGPGGRRLDEPGHSVSDYLKFKDLILRMLD 605
                    .|::..|||.   ...|.|:  :.:.|...|...|.|.    :|..:|.||:.:||.
Mouse   452 DDHEAETGIKSKEARKYI---FNCLDDMAQVNMTTDLEGSDMLVEK----ADRREFIDLLKKMLT 509

  Fly   606 FDPKTRVTPYYALQHNFF------------------------KR--------------------- 625
            .|...||||...|.|.|.                        ||
Mouse   510 IDADKRVTPIETLNHPFVTMTHLLDFPHSAHVKSCFQNMEICKRRVNMYDTVNQSKTPFITHVAP 574

  Fly   626 -TADEATNTSGAGATANAGAGGSGSSGAGGSSGGGVGGGLGASNSSSGAVSSSSAA-APTAATAA 688
             |:...|.|.....|.......:.||..           |..:|.....::..||. .|:||:.|
Mouse   575 STSTNLTMTFNNQLTTVHNQAPTTSSAT-----------LSLANPEVSILNYQSALYQPSAASMA 628

  Fly   689 ATA-------------------------------AGSSGSGSSVGGGSSAAQQQQAMPL----PL 718
            |.|                               .|...|.|...|.|  .:.:.|:|:    |.
Mouse   629 AVAPRSMPLQTGTAQICARPDPFQQALIVCPPGFQGLQASPSKHAGYS--VRMENAVPIVTQAPG 691

  Fly   719 PLPLPLPP-----LAGPGGASD-------GQCHGLLMHSVAANAMNNFSALSLQSNAHPPPSLAN 771
            ..||.:.|     .|.||||..       .|..|:..|:            |:|..|..|.::|
Mouse   692 AQPLQIQPGLLAQQAWPGGAQQILLPPAWQQLTGVATHT------------SVQHAAVIPETMA- 743

  Fly   772 SHHSTNSLGSLNHISPGSTGCHNNNSNSSNNNTRHSRLYGSNMVNMVGHHNSGSSNNHNSISYPH 836
               .|..|.                      :.|::..:||               ::|.|....
Mouse   744 ---GTQQLA----------------------DWRNTHAHGS---------------HYNPIMQQP 768

  Fly   837 AMECDPPQMPPPPPNGHGRMRVPAIMQLQPNSYA--------PNSVPYYGNMSSSSVAAAAAAAA 893
            |:......:|...|...|   |..:|:.||.|..        .:||   .|:|:..|.::.|.::
Mouse   769 ALLTGHVTLPAAQPLNVG---VAHVMRQQPTSTTSSRKSKQHQSSV---RNVSTCEVTSSQAISS 827

  Fly   894 AAAS---------HLMMTDSSVISASAAGGGQGGGNPGQNPVTPSAA------AFLFPSQPAGTL 943
            ...|         ...|..||...:::...|.|       .|..|..      ..:.|..|:.|:
Mouse   828 PQRSKRVKENTPPRCAMVHSSPACSTSVTCGWG-------DVASSTTRERQRQTIVIPDTPSPTV 885

  Fly   944 YGTALGSLSDLPLPMP-LPMS------------VPLQLPP--SSSSSVSSGSASVGSGGVGVGVV 993
            ....:.|.:|...... .|.|            |.:...|  .|||:.|..|....:|..|...:
Mouse   886 SVITISSDTDEEEEQKHAPTSTVSKQRKNVISCVTVHDSPYSDSSSNTSPYSVQQRTGHNGTNTL 950

  Fly   994 ----GQRRHITG------------PAAQVGI--------------SQSVGSGSSGSATGASSSDA 1028
                |...|.||            .|::|.:              |.|..|.||.:.|..|...:
Mouse   951 DTKGGLENHCTGNPRTIIVPPLKTQASEVLVECDSLGPAISASHHSSSFKSKSSSTVTSTSGHSS 1015

  Fly  1029 SSSSPMVGVCVQQNP 1043
            .|||..:.. .||.|
Mouse  1016 GSSSGAIAY-RQQRP 1029

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mnb	NP_728107.2	PKc_DYRK1	289..628	CDD:271128	137/406 (34%)
mnb	NP_728107.2	S_TKc	303..623	CDD:214567	130/338 (38%)
Hipk2	NP_034563.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	50..69		1/18 (6%)
		Transcriptional corepression	97..230		29/139 (21%)
		STKc_HIPK2	183..537	CDD:271129	134/364 (37%)
		Interaction with DAXX. /evidence=ECO:0000250	189..520		129/341 (38%)
		S_TKc	199..527	CDD:214567	130/338 (38%)
		Interaction with SKI and SMAD1. /evidence=ECO:0000250	539..844		65/376 (17%)
		Interaction with POU4F1. /evidence=ECO:0000269\|PubMed:15492043	752..897		33/172 (19%)
		Interaction with CTBP1. /evidence=ECO:0000269\|PubMed:14567915	774..876		22/114 (19%)
		Interaction with HMGA1. /evidence=ECO:0000269\|PubMed:11593421	787..897		24/119 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	792..847		11/57 (19%)
		Nuclear localization signal 1 (NLS1). /evidence=ECO:0000250	802..805		0/2 (0%)
		Nuclear localization signal 2 (NLS2). /evidence=ECO:0000250	832..835		0/2 (0%)
		Interaction with TP53 and TP73. /evidence=ECO:0000269\|PubMed:11780126	839..934		19/101 (19%)
		Localization to nuclear speckles	873..980		21/106 (20%)
		Required for localization to nuclear speckles. /evidence=ECO:0000250	873..980		21/106 (20%)
		Interaction with UBE2I. /evidence=ECO:0000269\|PubMed:10535925	873..907		6/33 (18%)
		SUMO interaction motifs (SIM), required for nuclear localization and kinase activity. /evidence=ECO:0000250	884..908		5/23 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	891..963		16/71 (23%)
		Interaction with AXIN1. /evidence=ECO:0000269\|PubMed:15526030	935..1050		22/96 (23%)
		Autoinhibitory domain (AID). /evidence=ECO:0000250	984..1196		13/47 (28%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	991..1058		13/40 (33%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167845483
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG0667
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R968
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	5	4.770