DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment mnb and Hipk1

DIOPT Version :9

Sequence 1:	NP_728107.2	Gene:	mnb / 32771	FlyBaseID:	FBgn0259168	Length:	1047	Species:	Drosophila melanogaster
Sequence 2:	NP_001288233.1	Gene:	Hipk1 / 15257	MGIID:	1314873	Length:	1210	Species:	Mus musculus

Alignment Length:	1161	Identity:	278/1161 - (23%)
Similarity:	423/1161 - (36%)	Gaps:	334/1161 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly   104 FLPKSAWDTVLLDVKRDVDEDGDRDRDRVDGNVLMVQGSSSSSSRMKAERALYYTMSAYGSSGGS 168
            |.|.|...:.....|:...|....|          |.|.||:..        |||.|.      :
Mouse     8 FSPPSVSSSAFCSAKKLKIEPSGWD----------VSGQSSNDK--------YYTHSK------T 48

  Fly   169 VDAAQGSGSGGGR--QRHAPLY--GRFVDAEDLPATHRDVMHHHSSPSSSSEVRAMQARIPNHFR 229
            :.|.||..|...:  ..:.|.|  |..:.|   ||....|:....|..|::......::...|
Mouse    49 LPATQGQASSSHQVANFNLPAYDQGLLLPA---PAVEHIVVTAADSSGSAATATFQSSQTLTH-- 108

  Fly   230 EPASGPLRKLSVDLIKTYKHINEVYYAKKKRRAQQTQGDDDSSNKKER---KLYN---------- 281
                    :.:|.|::.|:...      .||::::.:.:......:|.   .|.|
Mouse   109 --------RSNVSLLEPYQKCG------LKRKSEEVESNGSVQIIEEHPPLMLQNRTVVGAAATT 159

  Fly   282 ------DGYDDDNHDYIIKNGE---KFLDRYEIDSLIGKGSFGQVVKAYDHEEQCHVAIKIIKNK 337
                  ........||.:...|   ...:.||:...:|:|:||||.|.:....:..|||||:||.
Mouse   160 TTVTTKSSSSSGEGDYQLVQHEILCSMTNSYEVLEFLGRGTFGQVAKCWKRSTKEIVAIKILKNH 224

  Fly   338 KPFLNQAQIEVKLLEMMNRADAENKYYIVKLKRHFMWRNHLCLVFELLSYNLYDLLRNTNFRGVS 402
            ..:..|.||||.:|..::..:|: :|..|:....|..:||.|||||:|..||||.|:...|..:.
Mouse   225 PSYARQGQIEVSILSRLSSENAD-EYNFVRSYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLP 288

  Fly   403 LNLTRKFAQQLCTALLFLSTPELNIIHCDLKPENILLCNPKRS--AIKIVDFGSSCQLGQRIYH- 464
            |...|...||:.|||:.|.:  |.:||.|||||||:|.:|.|.  .:|::||||:..:.:.:..
Mouse   289 LKYIRPILQQVATALMKLKS--LGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKAVCST 351

  Fly   465 YIQSRFYRSPEVLLGIQYDLAIDMWSLGCILVEMHTGEPLFSGCNEVDQMNKIVEVLGMPPKYLL 529
            |:|||:||:||::||:.:..|||||||||::.|:..|.||:.|.:|.||:..|.:..|:|.:|||
Mouse   352 YLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLL 416

  Fly   530 DQAHKTRKFFD----------KIVADGSYVLK---KNQNGRKYKPPGSRKLHDI--LGVETGGPG 579
            ....||.:||:          ::.....:.|:   |::..|||.   ...|.|:  :.:.|...|
Mouse   417 SAGTKTTRFFNRDPNLGYPLWRLKTPEEHELETGIKSKEARKYI---FNCLDDMAQVNMSTDLEG 478

  Fly   580 GRRLDEPGHSVSDYLKFKDLILRMLDFDPKTRVTPYYALQHNFF--------------------- 623
            ...|.|.    :|..::.||:.:||..|...|:||...|.|.|.
Mouse   479 TDMLAEK----ADRREYIDLLKKMLTIDADKRITPLKTLNHQFVTMSHLLDFPHSSHVKSCFQNM 539

  Fly   624 ---KR---------------TADEATNTS---------------------GAGATANAGAGGSGS 649
               ||               |...|.|||                     .:.:||.|......:
Mouse   540 EICKRRVHMYDTVSQIKSPFTTHVAPNTSTNLTMSFSNQLNTVHNQASVLASSSTAAAATLSLAN 604

  Fly   650 SGAG---------GSSGGGVGG-----------------------------------GLGASNSS 670
            |...         .||...|.|                                   ||.|:...
Mouse   605 SDVSLLNYQSALYPSSAAPVPGVAQQGVSLQPGTTQICTQTDPFQQTFIVCPPAFQTGLQATTKH 669

  Fly   671 SGAVSSSSAAAPTAATAAATAAGSSGSGSSVGGGS------------SAAQQQQAMPLPLPLPLP 723
            ||.......|.|....|.|.......||....|..            :....|.|:|..|.....
Mouse   670 SGFPVRMDNAVPIVPQAPAAQPLQIQSGVLTQGSCTPLMVATLHPQVATITPQYAVPFTLSCAAG 734

  Fly   724 LPPL---------AGPGGASD-------GQCHGLLMH------SVAANAMNNFSALSLQSNAH-- 764
            .|.|         |.|||...       .|..|:.:|      :|...||.:...|:...|||
Mouse   735 RPALVEQTAAVLQAWPGGTQQILLPSAWQQLPGVALHNSVQPAAVIPEAMGSSQQLADWRNAHSH 799

  Fly   765 --------PPPSLANSHHSTNSLGSLN----HI---SPGSTGCHNNNSNSSNNNTRHS-RLYGSN 813
                    ..|||..:|.:..:...||    |:   ...|:.....|..|:..:::.| .:..|.
Mouse   800 GNQYSTIMQQPSLLTNHVTLATAQPLNVGVAHVVRQQQSSSLPSKKNKQSAPVSSKSSLEVLPSQ 864

  Fly   814 MVNMVGHHNSGSSNNHNSISYPHAMECDP---PQMPPPPPNGHGRMRVPAIM----QLQPNSYAP 871
            :.::||.....:::::||: .|...:..|   |..|.||      :.|..|.    :.:.|.|.|
Mouse   865 VYSLVGSSPLRTTSSYNSL-VPVQDQHQPIIIPDTPSPP------VSVITIRSDTDEEEDNKYKP 922

  Fly   872 NSVPYYGNMSSSSVAAAAAAAAAAASHLMMTDS-------------SVISASAAGGG---QGGGN 920
            ||             ::..|.:...|::.:.||             ..:||.....|   :|.|.
Mouse   923 NS-------------SSLKARSNVISYVTVNDSPDSDSSLSSPHPTDTLSALRGNSGTLLEGPGR 974

  Fly   921 PGQNPVTPSAAAFLFPSQPAGTLYG------TALGSLSDLPLPMPLPMSVPLQLPPSSSSSVSSG 979
            |..:.:  .....:.|  |..|..|      .|.|.||....|:                    .
Mouse   975 PAADGI--GTRTIIVP--PLKTQLGDCTVATQASGLLSSKTKPV--------------------A 1015

  Fly   980 SASVGSGGVGVGVVGQRRHITGPAA--QVGISQSVGSGSSGSATGASSSDASSSSP 1033
            |.|..|.|..:...|.|....|.:|  .:.:||:..|.|      ||:|...||:|
Mouse  1016 SVSGQSSGCCITPTGYRAQRGGASAVQPLNLSQNQQSSS------ASTSQERSSNP 1065

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mnb	NP_728107.2	PKc_DYRK1	289..628	CDD:271128	132/398 (33%)
mnb	NP_728107.2	S_TKc	303..623	CDD:214567	125/337 (37%)
Hipk1	NP_001288233.1	STKc_HIPK1	174..528	CDD:271130	129/363 (36%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	835..856		3/20 (15%)
		Nuclear localization signal 1 (NLS1). /evidence=ECO:0000250	844..847		0/2 (0%)
		Interaction with TP53. /evidence=ECO:0000269\|PubMed:12702766	885..1093		50/230 (22%)
		Required for localization to nuclear speckles. /evidence=ECO:0000250	891..998		26/129 (20%)
		SUMO interaction motifs (SIM), required for nuclear localization and kinase activity. /evidence=ECO:0000250	902..926		8/42 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	938..981		8/44 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1046..1069		10/26 (38%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1084..1104
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167845487
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG0667
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R968
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	5	4.770