Protein Alignment beta-Spec and Sptbn4

DIOPT Version :10

• Sequence 1: NP_001259660.1
• Gene: beta-Spec / 32746
• FlyBaseID: FBgn0250788
• Length: 2308
• Species: Drosophila melanogaster
• Sequence 2: XP_006540500.1
• Gene: Sptbn4 / 80297
• MGIID: 1890574
• Length: 2580
• Species: Mus musculus
• Alignment Length: 2554
• Identity: 1025/2554 - (40%)
• Similarity: 1436/2554 - (56%)
• Gaps: 292/2554 - (11%)

  Fly     9 RWD-PSQGPGNEYIDEYEYDGGNSSSRLFERSRIKALAEERESVQKKTFTKWVNSHLCRVNCRIA 72
            ||: |.:|     .|.......|:::.|||.|||||||:|||:||||||||||||||.||.|.|.
Mouse    44 RWESPDRG-----WDREPPAAANAAASLFECSRIKALADEREAVQKKTFTKWVNSHLARVGCHIG 103

  Fly    73 DLYVDMRDGKHLIKLLEVLSGERLPKPTKGKMRIHCLENVDKALQFLREQRVHLENIGSHDIVDG 137
            |||.|:|||..|.:||||||||:||:||:|:||||.|||||||||||:||||||||:||||||||
Mouse   104 DLYADLRDGFVLTRLLEVLSGEQLPRPTRGRMRIHSLENVDKALQFLKEQRVHLENVGSHDIVDG 168

  Fly   138 NASLNLGLIWTIILRFQIQDITIEEVDNKETKSAKDALLLWCQMKTAGYHNVNVRNFTTSWRDGL 202
            |..|.|||:||||||||||.|.||..||:||:|||||||||||||||||..||::||||||||||
Mouse   169 NHRLTLGLVWTIILRFQIQVIKIETEDNRETRSAKDALLLWCQMKTAGYPEVNIQNFTTSWRDGL 233

  Fly   203 AFNAIIHKHRPDLVQFEKLSKTNAIHNLNNAFDVAEDKLGLAKLLDAEDVFVEHPDEKSIITYVV 267
            ||||:||:||||||...||:|:||.:||..||..||..||||:|||.|||.:|.|||||||||||
Mouse   234 AFNALIHRHRPDLVDLSKLTKSNANYNLQRAFRTAEQHLGLARLLDPEDVNMEAPDEKSIITYVV 298

  Fly   268 TYYHYFSKLKQETVQGKRIGKVVGIAMENDKMVHDYENFTSDLLKWIETTIQSLGEREFENSLAG 332
            ::||||||:|...|:|||||||:...:|.||::..||...::||.||..|:..:..::|.|||:|
Mouse   299 SFYHYFSKMKALAVEGKRIGKVLDQVLEVDKIIERYEELAAELLAWIHRTVDLISNQKFANSLSG 363

  Fly   333 VQGQLAQFSNYRTIEKPPKFVEKGNLEVLLFTLQSKMRANNQKPYTPKEGKMISDINKAWERLEK 397
            ||.||..|:.|.|:|||.||.||||||||||::|||:||:|::.:.|:||..|.||:|||..|||
Mouse   364 VQQQLQAFTAYCTLEKPVKFQEKGNLEVLLFSIQSKLRAHNRRLFVPREGCGIWDIDKAWGELEK 428

  Fly   398 AEHERELALREELIRQEKLEQLAARFDRKASMRETWLSENQRLVSQDNFGFDLAAVEAAAKKHEA 462
            ||||||.|||.|||||||||.||.|||.|.:|||:||:||||||||||||::|.|||||.|||||
Mouse   429 AEHEREAALRAELIRQEKLELLAQRFDHKVAMRESWLNENQRLVSQDNFGYELPAVEAAMKKHEA 493

  Fly   463 IETDIFAYEERVQAVVAVCDELESERYHDVKRILLRKDNVMRLWTYLLELLRARRMRLEISLQLQ 527
            ||.||.|||||||.|..:...|.:|.|:|.:|:..::|:|:|.|..|..|:.|||.|||.:|.||
Mouse   494 IEADIAAYEERVQGVAELAQALAAEGYYDARRVAAQRDSVLRQWALLTGLVGARRTRLEQNLALQ 558

  Fly   528 QNFQEMLYILDNMEEIKQLLMTDDYGKHLMGVEDLLQKHSLVEADINILGERVKVVVQNSQKFLS 592
            :.||||:|::|.|||::..|::.:.|:||:..:||||||.|:|.||....|||:.:  |:.....
Mouse   559 KVFQEMVYMVDWMEEMQTQLLSRECGQHLVEADDLLQKHGLLEGDIAAQSERVEAL--NAAALRF 621

  Fly   593 DDPESYKPCDPEIIVSRVQQLEDAYAELVRLAVERRSRLEESRKLWQFYWDTADEENWIKEKEQI 657
            ...:.|:||||::|.:||..:....:||...|..||:.||.||.||....:..:.|:|.::||::
Mouse   622 SQLQGYQPCDPQVICNRVNHVHGCLSELQEQAARRRAELEASRSLWALLQELEEAESWARDKERL 686

  Fly   658 V-STDEVG---------HDLTTVNLMLSKHKALESEITSHDPQLQNVAKVGSELITEGHF---GA 709
            : :|...|         |||::...:|::||.|:.|:......||...:.|.||...|..   ||
Mouse   687 LEATSGSGGAAGTAGGAHDLSSTARLLAQHKILQGELGGRRALLQQALRRGEELAAAGGSVGPGA 751

  Fly   710 D-----RIKDRLKEILNKWDHLLDLTKYRRQRLENAVEYFQLFADADDVDNWMLDTLRIVSSEDV 769
            :     .:.:|......:|..|.:....|.:||:.|....|..||.|.:.:|:.|..|:.::.|.
Mouse   752 EPLHLAGLAERAASARRRWQRLEEAAARRERRLQEARALHQFGADLDGLLDWLRDAYRLAAAGDF 816

  Fly   770 GRDEANVQSLLKKHKDVADELKNYAEVIDALHKQAESLKLNEAEKAN-----VDKRLEAIDNRYK 829
            |.|||:.:.|.::|:.:..|::.:...:..|.:|..:  |..|..|.     :..|:...:..:.
Mouse   817 GHDEASSRRLARQHRALTGEVEAHRGPVGGLRRQLAT--LGGASGAGPLVVALQVRVVEAEQLFA 879

  Fly   830 ELTELAKLRKQRLLDALSLYKLMSEADGVEQWIKEKTKMLDTMTPGKDIEDVEIMKHRFEGFDKE 894
            |:||:|.||:|.|.|||::|::..|....|.||.||.:.|..|.....::|||:::||||..|:|
Mouse   880 EVTEVAALRRQWLRDALAVYRMFGEVHACELWIGEKEQWLLAMRVPDSLDDVEVVQHRFESLDQE 944

  Fly   895 MNANASRVAVVNQLARQLLHVEHPNSDEILERQNHLNQEWSTLREKAEAKMDDLKSAHGVQTFYI 959
            ||:...||..|||..::|:...||:|||:...|:|||..|:.:.|..|.:.:::.:...|:...:
Mouse   945 MNSLMGRVLDVNQTVQELVEGGHPSSDEVRSCQDHLNSRWNRIVELVEQRKEEMSAVLLVENHVL 1009

  Fly   960 ECRETISWIEDKKRILTETDSLEMDLTGVMTLQRRLSGMDRDLAAIQAKLSSLEREANSIEDEHP 1024
            |..|..:.:.:|:|      ::|........||.||||::..|.|::.:.::|..||..:.:..|
Mouse  1010 EVAEVRAQVREKRR------AVESAPRAGGALQWRLSGLEAALQALEPRQAALLEEAALLAERFP 1068

  Fly  1025 EEAKIIRERIAQIELIWEQLTQMLKERDSKLEEAGDLHRFLRDLDHFQTWLTKTQTDVASED--T 1087
            .:|..:.:...::...|..|....:.....:..||.|.|||||||.|..||.:.|....:.:  .
Mouse  1069 AQATRLHQGAEELGAEWGALAGAAQACGEAVAAAGRLQRFLRDLDTFLDWLVRAQEAAGAVEGPL 1133

  Fly  1088 PTSLPEAEKLLNQHQSIREEIDNYTEDYKNMMEYGER-LTSEGSTSDDPQYMFLRERLNALKDGW 1151
            |.||.||:.||.:|.:::||:|...|||..::...|. |.|||:.....  :.|.|.|..|:.||
Mouse  1134 PRSLEEADGLLARHAALKEEVDQREEDYARIVAASEALLASEGAELGPG--LALDEWLPHLEVGW 1196

  Fly  1152 EELHQMWENRQVLLSQSLDQQLFNRDARQTEVLLSQQEHFLSKDDTPVNLEQAENQLKRHEAFLT 1216
            .:|..:||.|:..|.|:...|||.||..|...:|..||..||..:.|..:|..|..:|||..|||
Mouse  1197 HKLLGLWEERREALVQAHVYQLFLRDLCQALAVLRNQEVALSGAELPCTVESVEEAMKRHRDFLT 1261

  Fly  1217 TMEANDDKINTLLQVADTLVEKDHFDADKIGKRAENITGRRDDNRQRALDQHEKLKNQVKLHEFL 1281
            |||.|..|:...:|.|::|:.:.:...::..:....:..:..:|:.||....:||.:|:.|..||
Mouse  1262 TMELNQQKMQVAVQAAESLLRQGNAYGEQAQEAVARLLEKSQENQLRAQQWMQKLLDQLVLQHFL 1326

  Fly  1282 QDLEELAEWVQEKYATSQDESYRSAKTIHSKWTRHQAFEAEIAANKERLFEAEKSAQELSKEKPE 1346
            :|..||..|:.||...::|.:...:..:|.:|.|||||.||:|.|||.|.:.|:..|:|.:||||
Mouse  1327 RDCHELDGWIHEKMLMARDGTREDSHKLHKRWLRHQAFMAELAQNKEWLEKIEREGQQLMQEKPE 1391

  Fly  1347 FKDVIEPKLKELAKQFDDLEVHTKEKGAMLFDANREVLVQQTCDDIDSYITDLEKQIVSGDTAND 1411
            ....:..||.|:.:.:.:||..|:.|...||:|::...:.|:..::|..:..:|.|:...|...|
Mouse  1392 LAASVRKKLGEIRQCWAELESTTQAKARQLFEASKADQLVQSFAELDKRLLHMESQLQDVDPGGD 1456

  Fly  1412 LTSVNILMQKQQVIQTQMAVKARQVEEIDKQTEYLQKTVPEEKIEPIVV--KKTAVLERFEKIKA 1474
            |.:||..::|.|.:::|:....|:|.|:..||..|    |.|:....:|  :::||.||..::..
Mouse  1457 LATVNSQLKKLQSMESQVEEWCREVGELQAQTAAL----PLEQASKELVGERQSAVGERLVRLLE 1517

  Fly  1475 PLLERQKALEKKKEAFQFCRDVEDEKLWIDEKLPVANSPDYGNSLFNVHVLKKKNQSLATEIDNH 1539
            ||.||::.|...||..|...|::||..|:.|:||:|...:.|..|..|....||||.|..||..|
Mouse  1518 PLQERRRLLLASKELHQVAHDLDDELAWVQERLPLAMQTERGTGLQAVQQHIKKNQGLRREIQAH 1582

  Fly  1540 EPRINAICNNGRKLIDEGHEDAKKFEALISDLTQKWQELKDAIENRRKHLLESEKVQQYFFDAQE 1604
            .||:..:......|......:|:........|...|..|::|.|.|::.|..:.:|:||:||..|
Mouse  1583 GPRLEEVLERAGVLASLRSPEAEAVRRGQEQLQSAWTGLREAAERRQQTLDAAFQVEQYYFDVAE 1647

  Fly  1605 AESWMSEQELYMMVEDRGKDEISAQNLMKKHENLEQSVEDYANTIRQLGEVARQFSGDDISSGDA 1669
            .|:|:.||||.||.||:||||.|...|:|||..|||.||:|..:|.||....|..........:.
Mouse  1648 VEAWLGEQELLMMSEDKGKDEQSTLQLLKKHLQLEQGVENYEESIAQLSRQCRALLEMGHPDSEQ 1712

  Fly  1670 VAVKQSQLDKLYAGLKDLAGERRARLNEALQLFMLSREVDDLEQWITDREVVAGSQELGQDFDHV 1734
            ::.:|||:|:||..||:|..|||..|.:...|:.|||:||:||.||.::||||||.||||||:||
Mouse  1713 ISRRQSQVDRLYVALKELGEERRVSLEQQYWLYQLSRQVDELEHWIAEKEVVAGSPELGQDFEHV 1777

  Fly  1735 TLLSERFNEFARDTEAVGGERVAKVNGIADNLIQAGHSDSATIAEWKDNLNESWQDLLELIETRT 1799
            ::|.|:|:|||.:|...|.||:|.||.:.|.||:.||:.:||:|||||.|||:|.:||||:.||.
Mouse  1778 SVLQEKFSEFASETGTAGRERLAAVNQMVDELIECGHTAAATMAEWKDGLNEAWAELLELMGTRA 1842

  Fly  1800 QMLAASRELHKFFHDCKDVLGRILEKQHGVSDELGR------DAGSVSTLQRKHYNFLQDLITLY 1858
            |:|||||||||||.|.:::.|:|.||:.    .|.|      ...|.|::||....|..||..|.
Mouse  1843 QLLAASRELHKFFSDARELQGQIEEKRR----RLPRLTAPPEPRPSASSMQRTLRAFEHDLQLLV 1903

  Fly  1859 SQVQQIQEESAKLQDAYAGDKAKEITNREQEVLHAWDNLQAMCDARKQKLADTGDLFRFFNMVRI 1923
            |||:|:||.:|:|:..|||:.|:.|.:||||||..|..|.|.|:..:..::.|.|..||.:..|.
Mouse  1904 SQVRQLQEGAAQLRTVYAGEHAEAIASREQEVLQGWKELLAACEDARLHVSSTADALRFHSQARD 1968

  Fly  1924 LMIWMEDLVRQMNTSEKPRDVSGVELLMNNHQSLKAEIDTREDNFGACISLGKELLTRNHYASAD 1988
            |:.||:.:..|:..::||||||.||:|||.||.||.|::.|......|..||:.||......:.:
Mouse  1969 LLSWMDGIAGQIGAADKPRDVSSVEVLMNYHQGLKTELEARVPELATCQELGRSLLLNKSAMADE 2033

  Fly  1989 IKDRLMTLSNSRNALLRRWEERWENLQLILEVYQFARDAAVAEAWLIAQEPYLLSSELGHTIDEV 2053
            |:.:|..|.:.:..:..:|:..||.||.:|||:|||::|.||:|||.||||.|.|.|||.::|||
Mouse  2034 IQAQLDKLGSRKEEVSEKWDRHWEWLQQMLEVHQFAQEAVVADAWLTAQEPLLQSRELGSSVDEV 2098

  Fly  2054 ENLIKKHEAFEKSAAAQEERFSALERLTTFE-LK------------------------------- 2086
            |.||::||||.|:|||.|||||:|.||||.| ||
Mouse  2099 EQLIRRHEAFRKAAAAWEERFSSLRRLTTIEKLKAEQSKQPPTPLLGRKFFGDPTELAAKAAPLL 2163

  Fly  2087 ---------------------------------EMK----------------------------- 2089
                                             |:|
Mouse  2164 RPGGYDRGLEPLARRASDTLSAEVRTRVGYVRQELKPERLQPRIDRLPETSGKVEPAAPTAAALD 2228

  Fly  2090 ------------------RRQELAEEAE---RQRIKEEQEAKAASEAAEQAKREAERRDDVDVGA 2133
                              .|||||:.||   |:|..|.||:....|...: :|..||::..|...
Mouse  2229 TTDTPGTPAATELVRPRSERQELADRAEELPRRRRSERQESVDQPEETAR-RRRPERQESADHEG 2292

  Fly  2134 SH-----------------------DDSAAKDTAVEFERV---------VEIQTERGGTPG---- 2162
            .|                       .:|:.::|....|.|         ||...|:...||
Mouse  2293 PHSLTLGRYEQMERRRERRERRIERQESSEQETPTRGELVKGKATLADIVEQLQEKEAGPGIPAG 2357

  Fly  2163 -----------------------------------------------AGEG-------------- 2166
                                                           .|||
Mouse  2358 VPSLPQPRELPPGRLPNGLEPPERTPRPDRPRARDRPKPRRRPRPREGGEGGGSRRSRSAPAQGG 2422

  Fly  2167 --------------HEGYVTRKHEWDSTTKKASNRSWDKVYMAAKAGRISFYKDQKGYKSNPELT 2217
                          |||::.||.|.|: .:|:|||||..:|.....|.:.||||.||..|..  |
Mouse  2423 SAPAPPPPPTHTVQHEGFLLRKRELDA-NRKSSNRSWVSLYCVLSKGELGFYKDSKGPASGG--T 2484

  Fly  2218 FRGEPSYDLQNAAIEIASDYTKKKHVLRVKLANGALFLLQAHDDTEMSQWVTSLKAQSDSTAVAA 2282
            ..|||...|..|..|:||||.|||||.:::..:|:.|||||.|:.||:.|:.::.......|..|
Mouse  2485 HGGEPLLSLHKATSEVASDYKKKKHVFKLQTQDGSEFLLQAKDEEEMNGWLEAVANSVAEHAEIA 2549

  Fly  2283 SRSQTLPATSQKDE--PKR 2299
            ...||||.||..||  |||
Mouse  2550 RWGQTLPTTSSTDEGNPKR 2568

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
beta-Spec	NP_001259660.1	CH_SPTB-like_rpt1	36..152	CDD:409095	91/115 (79%)
		CH_SPTB_like_rpt2	169..273	CDD:409097	76/103 (74%)
		Spectrin	298..408	CDD:395348	61/109 (56%)
		SPEC	422..521	CDD:197544	59/98 (60%)
		SPEC	525..740	CDD:238103	76/232 (33%)
		SPEC	639..846	CDD:238103	59/229 (26%)
		SPEC	848..1058	CDD:238103	59/209 (28%)
		SPEC	955..1169	CDD:238103	65/216 (30%)
		SPEC	1170..1380	CDD:238103	74/209 (35%)
		SPEC	1386..1484	CDD:197544	29/99 (29%)
		SPEC	1488..1699	CDD:238103	80/210 (38%)
		SPEC	1594..1806	CDD:238103	111/211 (53%)
		SPEC	1807..2015	CDD:238103	81/213 (38%)
		Spectrin	2018..>2078	CDD:395348	40/59 (68%)
		PH_beta_spectrin	2167..2274	CDD:269975	48/106 (45%)
Sptbn4	XP_006540500.1	CH_SF	40..183	CDD:469584	98/143 (69%)
		CH_SPTBN4_rpt2	185..314	CDD:409171	92/128 (72%)
		SPEC	332..555	CDD:238103	133/222 (60%)
		SPEC	450..664	CDD:238103	108/215 (50%)
		SPEC	790..1002	CDD:238103	70/213 (33%)
		SPEC	1106..1210	CDD:197544	40/105 (38%)
		SPEC	1216..1424	CDD:238103	74/207 (36%)
		SPEC	1322..1529	CDD:238103	71/210 (34%)
		SPEC	1531..1742	CDD:238103	80/210 (38%)
		SPEC	1637..1849	CDD:238103	111/211 (53%)
		Spectrin	1849..1948	CDD:395348	45/102 (44%)
		SPEC	1958..>2125	CDD:238103	79/166 (48%)
		PH_beta_spectrin	2437..2541	CDD:269975	48/106 (45%)