DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment beta-Spec and sptb

DIOPT Version :9

Sequence 1:	NP_001259660.1	Gene:	beta-Spec / 32746	FlyBaseID:	FBgn0250788	Length:	2308	Species:	Drosophila melanogaster
Sequence 2:	XP_005158906.1	Gene:	sptb / 58040	ZFINID:	ZDB-GENE-000906-1	Length:	2481	Species:	Danio rerio

Alignment Length:	2342	Identity:	1134/2342 - (48%)
Similarity:	1566/2342 - (66%)	Gaps:	81/2342 - (3%)

- Green bases have known domain annotations that are detailed below.

  Fly    30 NSSSRLFERS----RIKALAEERESVQKKTFTKWVNSHLCRVNCRIADLYVDMRDGKHLIKLLEV 90
            ::|:..|..|    |.:.|.:|||:||||||||||||.|.||:|||:|||:|:|||:.|||||||
Zfish   157 SASNICFNNSILDGRFRQLQDEREAVQKKTFTKWVNSILARVSCRISDLYLDLRDGRMLIKLLEV 221

  Fly    91 LSGERLPKPTKGKMRIHCLENVDKALQFLREQRVHLENIGSHDIVDGNASLNLGLIWTIILRFQI 155
            ||||||||||||:||||||||||||||||:||:|||||:|||||||||..|.|||||||||||||
Zfish   222 LSGERLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLILGLIWTIILRFQI 286

  Fly   156 QDITIE-----EVDNKETKSAKDALLLWCQMKTAGYHNVNVRNFTTSWRDGLAFNAIIHKHRPDL 215
            |||.:|     :...:||:|||||||||||||||||.|:|:.||||||:||:||||:||||||||
Zfish   287 QDIIVETGQADQTGRQETRSAKDALLLWCQMKTAGYPNINITNFTTSWKDGMAFNALIHKHRPDL 351

  Fly   216 VQFEKLSKTNAIHNLNNAFDVAEDKLGLAKLLDAEDVFVEHPDEKSIITYVVTYYHYFSKLKQET 280
            |.:..|.::|..|||..||:|||.|||:.||||.||||.|:|||||||||||.:||||||:|...
Zfish   352 VDYGNLKRSNPTHNLQQAFNVAEKKLGVTKLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKALA 416

  Fly   281 VQGKRIGKVVGIAMENDKMVHDYENFTSDLLKWIETTIQSLGEREFENSLAGVQGQLAQFSNYRT 345
            |:|||||||:..|:|.:||:..||..:||||.|||.||..|..|:..|||.|||.||..|::|||
Zfish   417 VEGKRIGKVLDQAIETEKMIEKYETLSSDLLTWIEQTIVVLNNRKLANSLTGVQQQLQAFNSYRT 481

  Fly   346 IEKPPKFVEKGNLEVLLFTLQSKMRANNQKPYTPKEGKMISDINKAWERLEKAEHERELALREEL 410
            :||||||.|||||||||||:||:|||||||.||||||.::||||||||||||||::||..||:||
Zfish   482 VEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPKEGALVSDINKAWERLEKAEYDRERVLRDEL 546

  Fly   411 IRQEKLEQLAARFDRKASMRETWLSENQRLVSQDNFGFDLAAVEAAAKKHEAIETDIFAYEERVQ 475
            ||||||||:|.||||||:||||||.||||||||||||:||.|||||.|||:||||||.|||||||
Zfish   547 IRQEKLEQMARRFDRKAAMRETWLMENQRLVSQDNFGYDLPAVEAAKKKHDAIETDIAAYEERVQ 611

  Fly   476 AVVAVCDELESERYHDVKRILLRKDNVMRLWTYLLELLRARRMRLEISLQLQQNFQEMLYILDNM 540
            |:||:..|||||||||.|||..||||::|||.||.|||:|||.||:.:|.||:.|||||:|:..|
Zfish   612 ALVALSKELESERYHDAKRIDARKDNILRLWDYLQELLKARRGRLDKNLTLQRIFQEMLHIISWM 676

  Fly   541 EEIKQLLMTDDYGKHLMGVEDLLQKHSLVEADINILGERVKVVVQNSQKFLSDDPESYKPCDPEI 605
            :|:|..|::.|:||||:.|||||||||||||||.:..||||.....:.||.:.|  |||||||::
Zfish   677 DEMKGRLLSPDFGKHLLEVEDLLQKHSLVEADIAVQAERVKSANAAALKFANGD--SYKPCDPQV 739

  Fly   606 IVSRVQQLEDAYAELVRLAVERRSRLEESRKLWQFYWDTADEENWIKEKEQIVSTDEVGHDLTTV 670
            |..|||.|:..|.||..||.:|::|||:||:||.|.|:.|:.|:||:|:|||.|:.:.|.|||:|
Zfish   740 IRDRVQHLDLCYQELCALAAQRKARLEQSRRLWNFLWEIAELESWIREREQIFSSLDYGKDLTSV 804

  Fly   671 NLMLSKHKALESEITSHDPQLQNVAKVGSELITEGHFGADRIKDRLKEILNKWDHLLDLTKYRRQ 735
            .::.|||...|.|:.:....|:.|...|..:|...|.|:.:::.|:.::..:|..|.:|..:|:|
Zfish   805 LILQSKHSVFEDELAARRDNLKQVMDEGESMIQIKHLGSPKVQQRMNDVQRQWQQLEELAAFRKQ 869

  Fly   736 RLENAVEYFQLFADADDVDNWMLDTLRIVSSEDVGRDEANVQSLLKKHKDVADELKNYAEVIDAL 800
            .|::...:||...||||:..|::|.:|.:||:|||.||...|.|||||:|:.||.......||||
Zfish   870 NLQDTQRFFQFQGDADDLKAWLVDAMRQMSSDDVGHDEYTTQRLLKKHRDLRDEAAKNGATIDAL 934

  Fly   801 HKQAESLKLNEAEKANVDKRLEAIDNRYKELTELAKLRKQRLLDALSLYKLMSEADGVEQWIKEK 865
            .|||.:|........::..||..|.:.|.||..|:.||:::|.|.::||.:.||.|..|.|:.:|
Zfish   935 SKQANALPEELRNTPDIQGRLNDIRDMYIELLTLSDLRQKKLDDTMALYTIFSETDACELWMGQK 999

  Fly   866 TKMLDTMTPGKDIEDVEIMKHRFEGFDKEMNANASRVAVVNQLARQLLHVEHPNSDEILERQNHL 930
            ...|..:...:::||:||:::|.....:||....:||..||:.|:||....||.:.::.:.|..|
Zfish  1000 ETWLVGLETPENLEDLEIVQNRLSILAQEMGNMQTRVDNVNKAAKQLEDSRHPQTKQVKDCQIRL 1064

  Fly   931 NQEWSTLREKAEAKMDDLKSAHGVQTFYIECRETISWIEDKKRILTETDSLEMDLTGVMTLQRRL 995
            |:.|...:...|.|...:.||..:..:.::|.||.|||::|.|::..|..|..||..|:|:||:|
Zfish  1065 NKRWEAFKAMVEDKKHRVDSALSLHNYDLDCDETESWIKEKTRVIESTQDLGNDLAAVITIQRKL 1129

  Fly   996 SGMDRDLAAIQAKLSSLEREANSIEDEHPEEAKIIRERIAQIELIWEQLTQMLKERDSKLEEAGD 1060
            .||:|||||||.||.||..||..:..||||.|..|..|..:::..|:.|.:.||:|:..|.|...
Zfish  1130 FGMERDLAAIQDKLDSLRDEAQKLVKEHPENASDILARQEELDAAWDTLKRTLKDREDSLGEVSK 1194

  Fly  1061 LHRFLRDLDHFQTWLTKTQTDVASEDTPTSLPEAEKLLNQHQSIREEIDNYTEDYKNMMEYGERL 1125
            |..||:|:|.||.||.|:|..|||||.|..|||||:|||.|.::|.::|.:.|||..:.:.|..:
Zfish  1195 LQTFLQDMDDFQAWLFKSQKAVASEDMPDGLPEAEQLLNLHDALRHDMDGHEEDYHRVKDTGAAV 1259

  Fly  1126 TSEGSTSDDPQYMFLRERLNALKDGWEELHQMWENRQVLLSQSLDQQLFNRDARQTEVLLSQQEH 1190
            ..  ...|||||..|.:||..|..||.|||:||::|:..|.|.|..|.|.|||:|.:.:|:.||:
Zfish  1260 IQ--GQEDDPQYQQLEQRLEGLDKGWGELHKMWDSRKNFLDQGLGFQQFMRDAKQADAILNNQEY 1322

  Fly  1191 FLSKDDTPVNLEQAENQLKRHEAFLTTMEANDDKINTLLQVADTLVEKDHFDADKIGKRAENITG 1255
            .|:..|.|..|:.||..||:||.|:|||:||.:||.:.|:....||:.::..:.|:..:..:|..
Zfish  1323 TLAHVDKPDTLDGAEKALKKHEDFVTTMDANKEKILSTLETGQRLVDSENLYSGKVKDKMSSIEE 1387

  Fly  1256 RRDDNRQRALDQHEKLKNQVKLHEFLQDLEELAEWVQEKYATSQDESYRSAKTIHSKWTRHQAFE 1320
            |...|:.:|.:...|||:..:|..|||:.::|..|:.||..|:||.||..|:.:||||.:||||.
Zfish  1388 RNKKNQDKAKEVSGKLKDNRELQHFLQNTQDLTLWINEKMLTAQDTSYDEARNLHSKWQKHQAFM 1452

  Fly  1321 AEIAANKERLFEAEKSAQELSKEKPEFKDVIEPKLKELAKQFDDLEVHTKEKGAMLFDANREVLV 1385
            ||:|:||:.|...:|..|||.:.||||:.:::.:|.:|.:.:|.||..|:||..:||||||..|.
Zfish  1453 AELASNKDWLHNIDKEGQELMESKPEFEPIVKDRLAKLHELWDKLESTTQEKARLLFDANRSELF 1517

  Fly  1386 QQTCDDIDSYITDLEKQIVSGDT---ANDLTSVNILMQKQQVIQTQMAVKARQVEEIDKQTEY-- 1445
            .|:..|:..::.:|::|: .||.   ..||||.|||::|.|:.:.|:..:||::||:.:..|.
Zfish  1518 DQSLADLKKWLAELQQQL-QGDVEEEVKDLTSANILLKKHQITENQVRDRARELEELQEAVEQHG 1581

  Fly  1446 -LQKTVPEEKIEPIVVKKTAVLERFEKIKAPLLERQKALEKKKEAFQFCRDVEDEKLWIDEKLPV 1509
             |::..||.:||...:::.     |:|:..||.:|:..||..|...||.||:.||.||::|:||:
Zfish  1582 SLREDQPELEIEQQNLQRD-----FQKLLTPLSQRKGKLEAAKAVHQFFRDLADEILWVNERLPM 1641

  Fly  1510 ANSPDYGNSLFNVHVLKKKNQSLATEIDNHEPRINAICNNGRKL--IDEGHEDAKKFEALISDLT 1572
            |.|.|:||:|..|.:|.||||||..|||.|:|||:.:...||::  ..||..:.::....:..|.
Zfish  1642 AMSDDHGNNLQTVQLLLKKNQSLQKEIDGHQPRIDEVLERGRRMAAAAEGSPEEERMSEEMKKLQ 1706

  Fly  1573 QKWQELKDAIENRRKHLLESEKVQQYFFDAQEAESWMSEQELYMMVEDRGKDEISAQNLMKKHEN 1637
            ..|.:|::.:..||:.|..|.:.|||:.||.::|:|:.||||||:.::..|||.||..::|:|..
Zfish  1707 VVWAQLQEEMAKRRERLYGSNEAQQYYNDADDSEAWIGEQELYMIADEMAKDEQSAMIMLKRHLV 1771

  Fly  1638 LEQSVEDYANTIRQLGEVARQFSGDDISSGDAVAVKQSQLDKLYAGLKDLAGERRARLNEALQLF 1702
            |:|:|:|||.:|:||.:.|::...::...|:|:..:|.|:||.|||||:||.:|:.:|:.....|
Zfish  1772 LKQTVDDYAYSIQQLADRAQKMLAEEHPDGEAIIRRQGQVDKQYAGLKELAEDRKKKLDHTYHHF 1836

  Fly  1703 MLSREVDDLEQWITDREVVAGSQELGQDFDHVTLLSERFNEFARDTEAVGGERVAKVNGIADNLI 1767
            :|||||:||||||.:|:|||.|||:|||.||||:|.::|.||||:|..||.|||..||.|.|.||
Zfish  1837 LLSREVEDLEQWIAERDVVASSQEMGQDLDHVTILRDKFREFARETGTVGQERVDTVNRIIDELI 1901

  Fly  1768 QAGHSDSATIAEWKDNLNESWQDLLELIETRTQMLAASRELHKFFHDCKDVLGRILEKQHGVSDE 1832
            :.|||:|||:|||||.:||||.||||||:||.|:|.:|.:|.|:|:|.|:::|.|.||::.:.::
Zfish  1902 EGGHSESATLAEWKDGVNESWADLLELIDTRAQLLTSSYDLLKYFYDGKELVGHIEEKKNELPED 1966

  Fly  1833 LGRDAGSVSTLQRKHYNFLQDLITLYSQVQQIQEESAKLQDAYAGDKAKEITNREQEVLHAWDNL 1897
            ||.|.....:..|.|..|.:|:.:|..||:|.||.:|:|...||||:|..|...|:||:.||..|
Zfish  1967 LGEDFSKAESFHRMHAAFERDISSLGKQVKQFQETAARLHAQYAGDQATAIQATEKEVVEAWKGL 2031

  Fly  1898 QAMCDARKQKLADTGDLFRFFNMVRILMIWMEDLVRQMNTSEKPRDVSGVELLMNNHQSLKAEID 1962
            ...|..|:::|.:|.|.||||.|||.||.|||.:::|:.|.|||||||.|||||..||.::|||:
Zfish  2032 LDACAGRRKQLEETADKFRFFTMVRDLMAWMESILQQIETQEKPRDVSSVELLMKYHQGIRAEIE 2096

  Fly  1963 TREDNFGACISLGKELLTRNHYASADIKDRLMTLSNSRNALLRRWEERWENLQLILEVYQFARDA 2027
            ||...|..|:.||:.||.|.|..||:||::||.|...|..::.:|::||:.|:|:|||.||||||
Zfish  2097 TRGPKFNQCVQLGQALLERKHKDSAEIKEKLMQLVEKRKEMMLKWDDRWDWLRLLLEVCQFARDA 2161

  Fly  2028 AVAEAWLIAQEPYLLSSELGHTIDEVENLIKKHEAFEKSAAAQEERFSALERLTTFELKEMKRR- 2091
            :||||||||||||:.|.::|.|:||||.|:|:|||||||.|..||||||||||||.||.|::::
Zfish  2162 SVAEAWLIAQEPYVASKDVGQTVDEVEKLLKRHEAFEKSTATWEERFSALERLTTLELLEIRKQQ 2226

  Fly  2092 ---QELAEEAERQRIKEE----QEAKAASEAAEQAKRE-----------------------AERR 2126
               |:..:|||::..:|:    :|:.......||:...                       :|.:
Zfish  2227 QEIQQYRQEAEKESRREDTGFAEESSQLYPTEEQSLSGLGVIEPSSGGVDGTAGESTVPLISEMQ 2291

  Fly  2127 DDVDVGASHDDSAAKDTAVEFERVVEIQTERGGTPGAGEGHEGYVTRKHEWDSTTKKASNRSWDK 2191
            :.|.:  ..|.|.:.....|.|:...:.||  .|.......||.:.||||.:...|||.||||:.
Zfish  2292 ESVSL--ELDPSTSIQVTKETEKAASLPTE--STQVQPVLMEGTLARKHELEGPNKKAPNRSWNN 2352

  Fly  2192 VYMAAKAGRISFYKDQKGYKSNPELTFRGEPSYDLQNAAIEIASDYTKKKHVLRVKLANGALFLL 2256
            :|...|.|.:|.|||.|.:..:  :||.||....|.|::.||.::|.|||.|.:::|.:|:.:|.
Zfish  2353 LYCVLKPGHLSIYKDAKSFSHS--VTFHGEEPLTLTNSSCEILTNYKKKKQVFKLRLGDGSEYLF 2415

  Fly  2257 QAHDDTEMSQWVTSLKAQSD----------------STAVAASRSQTLPATSQKDEPKRRSFFTL 2305
            |..|:.|:..|..:::..:.                |....:|.:..:|:| :||:.||.|.|..
Zfish  2416 QCKDEEELQNWTQAIEQAAQVQIEEPVAGPSGVKALSLPPPSSTTLEVPST-KKDKEKRFSLFAK 2479

  Fly  2306 KK 2307
            ||
Zfish  2480 KK 2481

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
beta-Spec	NP_001259660.1	CH	51..149	CDD:278723	82/97 (85%)
		CH	170..269	CDD:278723	73/98 (74%)
		Spectrin	298..408	CDD:278843	76/109 (70%)
		SPEC	422..521	CDD:197544	78/98 (80%)
		SPEC	525..740	CDD:238103	98/214 (46%)
		SPEC	639..846	CDD:238103	78/206 (38%)
		SPEC	848..1058	CDD:238103	78/209 (37%)
		SPEC	955..1169	CDD:238103	97/213 (46%)
		SPEC	1170..1380	CDD:238103	85/209 (41%)
		SPEC	1386..1484	CDD:197544	31/103 (30%)
		SPEC	1488..1699	CDD:238103	88/212 (42%)
		SPEC	1594..1806	CDD:238103	114/211 (54%)
		SPEC	1807..2015	CDD:238103	93/207 (45%)
		Spectrin	2018..>2078	CDD:278843	44/59 (75%)
		PH	2167..2275	CDD:278594	41/107 (38%)
		PH_beta_spectrin	2167..2274	CDD:269975	41/106 (39%)
sptb	XP_005158906.1	CH	182..285	CDD:237981	87/102 (85%)
		CH	306..409	CDD:237981	75/102 (74%)
		Spectrin	434..544	CDD:278843	76/109 (70%)
		Spectrin	554..656	CDD:278843	79/101 (78%)
		SPEC	662..873	CDD:238103	98/212 (46%)
		SPEC	774..980	CDD:238103	78/205 (38%)
		SPEC	982..1191	CDD:238103	78/208 (38%)
		SPEC	1088..1299	CDD:238103	96/212 (45%)
		SPEC	1303..1512	CDD:238103	85/208 (41%)
		SPEC	1515..1723	CDD:238103	76/213 (36%)
		SPEC	1622..1830	CDD:238103	87/207 (42%)
		SPEC	1728..1940	CDD:238103	114/211 (54%)
		SPEC	1941..2162	CDD:238103	103/220 (47%)
		Spectrin	2156..>2210	CDD:278843	39/53 (74%)
		PH	2326..2433	CDD:278594	41/108 (38%)
		PH_beta_spectrin	2328..2433	CDD:269975	41/106 (39%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	186	1.000	Domainoid score	I3289
eggNOG	1	0.900	-	-		E2759_KOG0517
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2354
Inparanoid	1	1.050	2452	1.000	Inparanoid score	I29
OMA	1	1.010	-	-		QHG52262
OrthoDB	1	1.010	-	-		D15759at33208
OrthoFinder	1	1.000	-	-		FOG0000586
OrthoInspector	1	1.000	-	-		otm26043
orthoMCL	1	0.900	-	-		OOG6_100348
Panther	1	1.100	-	-	O	PTHR11915
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3302
SonicParanoid	1	1.000	-	-		X1255
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	15	14.870