DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment beta-Spec and Sptb

DIOPT Version :9

Sequence 1:	NP_001259660.1	Gene:	beta-Spec / 32746	FlyBaseID:	FBgn0250788	Length:	2308	Species:	Drosophila melanogaster
Sequence 2:	NP_038703.3	Gene:	Sptb / 20741	MGIID:	98387	Length:	2329	Species:	Mus musculus

Alignment Length:	2341	Identity:	1114/2341 - (47%)
Similarity:	1543/2341 - (65%)	Gaps:	75/2341 - (3%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 RWDPSQGPGNEYIDEYEYDGGNSSSRLFERSRIKALAEERESVQKKTFTKWVNSHLCRVNCRIAD 73
            |||   .|.:      |.|..|||:||||||||||||:|||.||||||||||||||.||:|||:|
Mouse    22 RWD---APDD------ELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRISD 77

  Fly    74 LYVDMRDGKHLIKLLEVLSGERLPKPTKGKMRIHCLENVDKALQFLREQRVHLENIGSHDIVDGN 138
            ||.|:|||:.|||||||||||.||:|||||||||||||||||||||:||||||||:|||||||||
Mouse    78 LYKDLRDGRMLIKLLEVLSGEMLPRPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGN 142

  Fly   139 ASLNLGLIWTIILRFQIQDITIEEVDNKETKSAKDALLLWCQMKTAGYHNVNVRNFTTSWRDGLA 203
            ..|.||||||||||||||||.::..:.:||:|||||||||||||||||.:|||.|||:||:||||
Mouse   143 HRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLA 207

  Fly   204 FNAIIHKHRPDLVQFEKLSKTNAIHNLNNAFDVAEDKLGLAKLLDAEDVFVEHPDEKSIITYVVT 268
            |||:||||||||:.|:||..:||.|||.:||||||.:||:..|||.||||.|:|||||||||||.
Mouse   208 FNALIHKHRPDLIDFDKLKDSNARHNLEHAFDVAERQLGIIPLLDPEDVFTENPDEKSIITYVVA 272

  Fly   269 YYHYFSKLKQETVQGKRIGKVVGIAMENDKMVHDYENFTSDLLKWIETTIQSLGEREFENSLAGV 333
            :||||||:|...|:|||:|||:..|:|.:||:..|....||||.|||.||..|..|:|.|||:||
Mouse   273 FYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTISVLNSRKFANSLSGV 337

  Fly   334 QGQLAQFSNYRTIEKPPKFVEKGNLEVLLFTLQSKMRANNQKPYTPKEGKMISDINKAWERLEKA 398
            |.||..||.|||:||||||.|||||||||||:||:|||||||.|||.:||::||||:|||.||:|
Mouse   338 QQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEA 402

  Fly   399 EHERELALREELIRQEKLEQLAARFDRKASMRETWLSENQRLVSQDNFGFDLAAVEAAAKKHEAI 463
            |::||||||.||||||||||||.||||||:||||||:||||||:|||||:||||||||.||||||
Mouse   403 EYQRELALRSELIRQEKLEQLARRFDRKAAMRETWLNENQRLVTQDNFGYDLAAVEAAKKKHEAI 467

  Fly   464 ETDIFAYEERVQAVVAVCDELESERYHDVKRILLRKDNVMRLWTYLLELLRARRMRLEISLQLQQ 528
            |||..||||||:|:..:..|||.|.|||.|||:.||||::|||:||.||||:||.|||.:|.||:
Mouse   468 ETDTAAYEERVKALEDLAQELEKENYHDQKRIIARKDNILRLWSYLQELLRSRRQRLEATLALQK 532

  Fly   529 NFQEMLYILDNMEEIKQLLMTDDYGKHLMGVEDLLQKHSLVEADINILGERVKVVVQNSQKFLSD 593
            .||:||:.:|.|:|||..:::.::||||:.||||||||.|:||||.|.|::||.:...:.:|.
Mouse   533 LFQDMLHSIDWMDEIKAHILSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKAITAATLQFA-- 595

  Fly   594 DPESYKPCDPEIIVSRVQQLEDAYAELVRLAVERRSRLEESRKLWQFYWDTADEENWIKEKEQIV 658
            :.:.|:||||::|..||..||..::||..:|..|:::||:|::||:|:|:..:.|:||||||||.
Mouse   596 EGKGYQPCDPQVIQDRVSHLEQCFSELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIY 660

  Fly   659 STDEVGHDLTTVNLMLSKHKALESEITSHDPQLQNVAKVGSELITEGHFGADRIKDRLKEILNKW 723
            |:.:.|.|||:|.::..||||.|.|:...|..|:.:.:...:::.:..||..:|:.|:||:..:|
Mouse   661 SSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLKQIFQEADDMVAQKQFGHPQIETRVKEVSAQW 725

  Fly   724 DHLLDLTKYRRQRLENAVEYFQLFADADDVDNWMLDTLRIVSSEDVGRDEANVQSLLKKHKDVAD 788
            |||.:|..:|::.|::|..:||...||||:..|:.|..|::|.||||:||...::|.||||:..:
Mouse   726 DHLKELAAFRKKDLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKEFLE 790

  Fly   789 ELKNYAEVIDALHKQAESLKLNEAEKANVDKRLEAIDNRYKELTELAKLRKQRLLDALSLYKLMS 853
            ||:....|::.|..||:.......:..:|..||:|:...|:::...|:||..:|.:||.||.:..
Mouse   791 ELEESRGVMEHLEHQAQGFPEEFRDSPDVTNRLQALRKLYQQVLTQAELRGHKLQEALDLYTVFG 855

  Fly   854 EADGVEQWIKEKTKMLDTMTPGKDIEDVEIMKHRFEGFDKEMNANASRVAVVNQLARQLLHVEHP 918
            |:|..|.|:.||.|.||.|.....:||:|:::|||:..|:||....:::..||..|..|:...||
Mouse   856 ESDACELWMTEKGKWLDQMDIPNTLEDLEVVQHRFDILDQEMKTLMAQIDGVNLAANNLVESGHP 920

  Fly   919 NSDEILERQNHLNQEWSTLREKAEAKMDDLKSAHGVQTFYIECRETISWIEDKKRILTETDSLEM 983
            .|.|:.:.|:.||:.|...:.....:.:.:.||..|..:.::|.||..||.||.:::..|..|..
Mouse   921 RSGEVKQYQDRLNKRWQAFQAVVSEQREAVDSALRVNNYCVDCEETSKWIMDKTKVVESTKDLGQ 985

  Fly   984 DLTGVMTLQRRLSGMDRDLAAIQAKLSSLEREANSIEDEHPEEAKIIRERIAQIELIWEQLTQML 1048
            ||.||:.:||:|||::||:.||:.::|:||||:..:.:.|||:.:.|.:|.|.:|.:|:.|...|
Mouse   986 DLAGVIAIQRKLSGLERDVLAIRDRVSALERESQYLMESHPEQKEDIGQRQADVEKLWKGLQDAL 1050

  Fly  1049 KERDSKLEEAGDLHRFLRDLDHFQTWLTKTQTDVASEDTPTSLPEAEKLLNQHQSIREEIDNYTE 1113
            :.::..|.||..|..||:|||.|:.||:..|..|||||.|.||||||:||.||.:|:||||.:.:
Mouse  1051 QGQELSLGEASKLQAFLQDLDDFKAWLSMAQKAVASEDMPESLPEAEQLLQQHAAIKEEIDAHRD 1115

  Fly  1114 DYKNMMEYGERLTSEGSTSDDPQYMFLRERLNALKDGWEELHQMWENRQVLLSQSLDQQLFNRDA 1178
            ||..:...||::. ||.|  ||.|..|.:||..|...|:.|.:|||:|...|:|.|..|.|.:||
Mouse  1116 DYHRVKASGEKVI-EGQT--DPDYQLLGQRLEGLDTDWDALRRMWESRGNTLTQCLGFQEFQKDA 1177

  Fly  1179 RQTEVLLSQQEHFLSKDDTPVNLEQAENQLKRHEAFLTTMEANDDKINTLLQVADTLVEKDHFDA 1243
            :|.|.:||.||:.|:..:.|.:|..||..:::.|.||.:||.|.|||.:.:...:.||.:.:..:
Mouse  1178 KQAEAILSNQEYTLAHLEPPDSLAAAEAGIRKFEDFLVSMENNRDKILSPVDSGNKLVAEGNLYS 1242

  Fly  1244 DKIGKRAENITGRRDDNRQRALDQHEKLKNQVKLHEFLQDLEELAEWVQEKYATSQDESYRSAKT 1308
            :||.::.:.|..|...|.::|.:....||:.::|..|||:.:||..|:.:|..||.|.||..|:.
Mouse  1243 NKIMEKVQLIEDRHKKNNEKAQEATVLLKDNLELQNFLQNCKELTLWINDKLLTSPDVSYDEARN 1307

  Fly  1309 IHSKWTRHQAFEAEIAANKERLFEAEKSAQELSKEKPEFKDVIEPKLKELAKQFDDLEVHTKEKG 1373
            :|:||.:||||.||:|:::..|...:...::|..|||:||||:..:|:.|.|.:::|:...|.|.
Mouse  1308 LHNKWMKHQAFMAELASHQGWLENIDAEGRQLMAEKPQFKDVVSERLEALHKLWEELQSTAKAKA 1372

  Fly  1374 AMLFDANREVLVQQTCDDIDSYITDLEKQIVSGDTANDLTSVNILMQKQQVIQTQMAVKARQVEE 1438
            ..|..|....|..||..|::.:|..:|.|:.|.|...|||:||.::.|.:.::.|:.::..::||
Mouse  1373 EQLSAARSSDLRLQTHADLNKWIGAMEDQLRSDDLGKDLTTVNRMLAKLKRVEEQVNLRKEELEE 1437

  Fly  1439 IDKQTEYLQKTVPEEKIEPIVVKKTAVLERFEKIKAPLLERQKALEKKKEAFQFCRDVEDEKLWI 1503
            :......|.....:..:        ::.:||..:..||..|:|.||..|...|..||:|||.||:
Mouse  1438 LFADAPSLGAEAGDTDM--------SIEKRFLDLLEPLGRRKKQLELSKAKLQISRDLEDETLWV 1494

  Fly  1504 DEKLPVANSPDYGNSLFNVHVLKKKNQSLATEIDNHEPRINAICNNGRKLIDEGHEDAKKFEALI 1568
            :|:||:|.|.|||.:|..|.:..||||:|..||..|.||:..:...|::|:.....|.:..|..:
Mouse  1495 EERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHAPRVEDVLRRGQELVKAAEIDCQDIEERL 1559

  Fly  1569 SDLTQKWQELKDAIENRRKHLLESEKVQQYFFDAQEAESWMSEQELYMMVEDRGKDEISAQNLMK 1633
            ..|...|..|::|...|.:.|.::.:.|||:.||.|||:|:||||||:..::..|||..|..::|
Mouse  1560 GHLQSSWDTLREAAAGRLQRLRDAHEAQQYYLDAGEAEAWISEQELYVFSDEPPKDEEGAIVMLK 1624

  Fly  1634 KHENLEQSVEDYANTIRQLGEVARQFSGDDISSGDAVAVKQSQLDKLYAGLKDLAGERRARLNEA 1698
            :|...:::||:|...|:||...|:.........|:.:...|.|:||.||||||:|.|||.||...
Mouse  1625 RHLRQQRTVEEYGRNIKQLAGRAQSLLSAGHPEGEQIIRLQGQVDKQYAGLKDMAEERRRRLENM 1689

  Fly  1699 LQLFMLSREVDDLEQWITDREVVAGSQELGQDFDHVTLLSERFNEFARDTEAVGGERVAKVNGIA 1763
            ..||.|.||.||||||||::|:||.|||:||||||||:|.::|.:|||:|.|:|.|||..||.|.
Mouse  1690 YHLFQLKREADDLEQWITEKEMVASSQEMGQDFDHVTMLRDKFRDFARETGAIGQERVDNVNTII 1754

  Fly  1764 DNLIQAGHSDSATIAEWKDNLNESWQDLLELIETRTQMLAASRELHKFFHDCKDVLGRILEKQHG 1828
            :.||.||||::||||||||.||:.|.||||||:||.|:||||.:||::|:...::||.|.||...
Mouse  1755 ERLIDAGHSEAATIAEWKDGLNDMWADLLELIDTRMQLLAASYDLHRYFYTGTEILGLIDEKHRE 1819

  Fly  1829 VSDELGRDAGSVSTLQRKHYNFLQDLITLYSQVQQIQEESAKLQDAYAGDKAKEITNREQEVLHA 1893
            :.:::|.||.:..:..|.|..|.::|..|..||||.|:.:.:||.||||:||..|.::||||..|
Mouse  1820 LPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKADAIQSKEQEVSAA 1884

  Fly  1894 WDNLQAMCDARKQKLADTGDLFRFFNMVRILMIWMEDLVRQMNTSEKPRDVSGVELLMNNHQSLK 1958
            |..|...|..|:.:|.||.|.||||:|||.|:.|||.::||:.|.|:|||||.||||:..||.:|
Mouse  1885 WQALLDACAGRRAQLVDTADKFRFFSMVRDLLSWMESIIRQIETQERPRDVSSVELLLKYHQGIK 1949

  Fly  1959 AEIDTREDNFGACISLGKELLTRNHYASADIKDRLMTLSNSRNALLRRWEERWENLQLILEVYQF 2023
            |||:||..||..|:.||:.||.|.|.||.:|:::|..:.:.|..:..:||.|.:.|.::|||.||
Mouse  1950 AEINTRAKNFSTCLELGESLLQRQHQASDEIREKLQQVISRRQEMNDKWEARSDRLHMLLEVCQF 2014

  Fly  2024 ARDAAVAEAWLIAQEPYLLSSELGHTIDEVENLIKKHEAFEKSAAAQEERFSALERLTTFELKEM 2088
            :|||:|||||||||||||.|.:.|||:|.||.|||:|||||||.|:..|||:|||:.||.||||.
Mouse  2015 SRDASVAEAWLIAQEPYLASRDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKER 2079

  Fly  2089 KRRQELAEEAERQRIKEEQEAKAASEAAEQAKREAERRDDVD-------------VGASHDDSAA 2140
            :..:...||...|    |:|.:.|.||.:......||....:             :...|.|...
Mouse  2080 QTPERPTEEPGPQ----EEEGETAGEAPQVHHAATERTSPGEERGPWPQDLQPPPLPGHHKDEQE 2140

  Fly  2141 KDTAVE------FERVVEIQTERGGTPGA-----GEGH----EGYVTRKHEWDSTTKKASNRSWD 2190
            |....|      ..:|::.....|..|..     ..||    |||:.|||:.:...||||||||:
Mouse  2141 KSVGDERPATEPLFKVLDTPLSEGDEPTTLPAQRDLGHTVQMEGYLGRKHDLEGPNKKASNRSWN 2205

  Fly  2191 KVYMAAKAGRISFYKDQKGYKSNPELTFRGEPSYDLQNAAIEIASDYTKKKHVLRVKLANGALFL 2255
            .:|...:..:::||||.|.....  :.:.||....|::|..|||.:|.|||||.:::|:||:.:|
Mouse  2206 NLYCVLRNSQLTFYKDAKNLALG--VPYHGEEPLALRHAICEIAVNYKKKKHVFKLRLSNGSEWL 2268

  Fly  2256 LQAHDDTEMSQWVTSLKAQSDSTAVAASRS-----QTLPATS---------QKDEPKRRSFFTLK 2306
            ....|:.||..|:     ||.|||:..|:|     |:||..|         :|::.||.|||..|
Mouse  2269 FHGKDEEEMLMWL-----QSMSTAINESQSIRVKAQSLPLPSLAGPDASVGKKEKEKRFSFFPKK 2328

  Fly  2307 K 2307
            |
Mouse  2329 K 2329

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
beta-Spec	NP_001259660.1	CH	51..149	CDD:278723	83/97 (86%)
		CH	170..269	CDD:278723	75/98 (77%)
		Spectrin	298..408	CDD:278843	75/109 (69%)
		SPEC	422..521	CDD:197544	73/98 (74%)
		SPEC	525..740	CDD:238103	90/214 (42%)
		SPEC	639..846	CDD:238103	75/206 (36%)
		SPEC	848..1058	CDD:238103	74/209 (35%)
		SPEC	955..1169	CDD:238103	93/213 (44%)
		SPEC	1170..1380	CDD:238103	74/209 (35%)
		SPEC	1386..1484	CDD:197544	24/97 (25%)
		SPEC	1488..1699	CDD:238103	83/210 (40%)
		SPEC	1594..1806	CDD:238103	115/211 (55%)
		SPEC	1807..2015	CDD:238103	92/207 (44%)
		Spectrin	2018..>2078	CDD:278843	43/59 (73%)
		PH	2167..2275	CDD:278594	42/111 (38%)
		PH_beta_spectrin	2167..2274	CDD:269975	42/110 (38%)
Sptb	NP_038703.3	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..30		4/16 (25%)
		Actin-binding	2..275		195/261 (75%)
		CH	55..158	CDD:237981	88/102 (86%)
		CH	177..273	CDD:214479	72/95 (76%)
		Spectrin	302..411	CDD:278843	74/108 (69%)
		Spectrin 1. /evidence=ECO:0000255	303..411		73/107 (68%)
		Spectrin	422..526	CDD:278843	77/103 (75%)
		Spectrin 3. /evidence=ECO:0000255	529..635		48/107 (45%)
		SPEC	530..742	CDD:238103	90/213 (42%)
		Spectrin 4. /evidence=ECO:0000255	638..741		40/102 (39%)
		SPEC	641..848	CDD:238103	75/206 (36%)
		Spectrin 5. /evidence=ECO:0000255	744..846		36/101 (36%)
		SPEC	745..954	CDD:238103	72/208 (35%)
		Spectrin 6. /evidence=ECO:0000255	853..950		31/96 (32%)
		Spectrin 7. /evidence=ECO:0000255	958..1058		37/99 (37%)
		SPEC	962..1168	CDD:238103	93/208 (45%)
		Spectrin 8. /evidence=ECO:0000255	1062..1165		51/105 (49%)
		SPEC	1170..1379	CDD:238103	74/208 (36%)
		Spectrin 9. /evidence=ECO:0000255	1170..1258		30/87 (34%)
		Spectrin 10. /evidence=ECO:0000255	1275..1376		39/100 (39%)
		SPEC	1385..1475	CDD:197544	24/97 (25%)
		Spectrin 11. /evidence=ECO:0000255	1389..1463		18/81 (22%)
		Spectrin 12. /evidence=ECO:0000255	1481..1582		38/100 (38%)
		SPEC	1485..1690	CDD:238103	82/204 (40%)
		Spectrin 13. /evidence=ECO:0000255	1584..1688		45/103 (44%)
		SPEC	1691..1901	CDD:238103	111/209 (53%)
		SPEC	1798..2005	CDD:238103	92/206 (45%)
		Spectrin 15. /evidence=ECO:0000255	1798..1899		39/100 (39%)
		Spectrin 16. /evidence=ECO:0000255	1906..2006		49/99 (49%)
		Spectrin 17. /evidence=ECO:0000255	2013..2073		42/59 (71%)
		Spectrin	2013..>2070	CDD:278843	41/56 (73%)
		PH	2180..2288	CDD:278594	45/114 (39%)
		PH_beta_spectrin	2182..2287	CDD:269975	44/111 (40%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	191	1.000	Domainoid score	I3229
eggNOG	1	0.900	-	-		E2759_KOG0517
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG52262
OrthoDB	1	1.010	-	-		D15759at33208
OrthoFinder	1	1.000	-	-		FOG0000586
OrthoInspector	1	1.000	-	-		otm43240
orthoMCL	1	0.900	-	-		OOG6_100348
Panther	1	1.100	-	-	O	PTHR11915
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3302
SonicParanoid	1	1.000	-	-		X1255
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	14	13.820