Protein Alignment beta-Spec and kst

DIOPT Version :10

• Sequence 1: NP_001259660.1
• Gene: beta-Spec / 32746
• FlyBaseID: FBgn0250788
• Length: 2308
• Species: Drosophila melanogaster
• Sequence 2: XP_061506980.1
• Gene: kst / 1274583
• VectorBaseID: AGAMI1_004241
• Length: 4434
• Species: Anopheles gambiae
• Alignment Length: 2445
• Identity: 761/2445 - (31%)
• Similarity: 1233/2445 - (50%)
• Gaps: 269/2445 - (11%)

  Fly    36 FERSRIKALAEERESVQKKTFTKWVNSHLCRVNCRIADLYVDMRDGKHLIKLLEVLSGERLPKPT 100
            ||..|||.|.|||..:||||||||:||.|.:....:.||::|:.||..|:||||::|||:|.||.
Mosquito    40 FENERIKTLQEERLHIQKKTFTKWMNSFLVKAKMEVDDLFIDLADGIKLLKLLEIISGEKLGKPN 104

  Fly   101 KGKMRIHCLENVDKALQFLREQRVHLENIGSHDIVDGNASLNLGLIWTIILRFQIQDITI---EE 162
            .|:||:|.:|||:|:|.|| ..:|.||:||:.||||.|..|.||||||||||||||:|.|   ||
Mosquito   105 SGRMRVHKIENVNKSLAFL-HTKVRLESIGAEDIVDHNPRLILGLIWTIILRFQIQEIEIDVDEE 168

  Fly   163 VDNKETKSAKDALLLWCQMKTAGYHNVNVRNFTTSWRDGLAFNAIIHKHRPDLVQFEKLSKTNAI 227
            .::.|.||||||||||||.||.||.:|::.:||.|||.||.|||:||.|||||..:..|.....|
Mosquito   169 NESSEKKSAKDALLLWCQRKTHGYQHVHITDFTNSWRSGLGFNALIHSHRPDLFDYNSLMPGRNI 233

  Fly   228 HNLNNAFDVAEDKLGLAKLLDAEDVFVEHPDEKSIITYVVTYYHYFSKLKQETVQGKRIGKVVGI 292
            .|||:||||||.:||:.:||||||:....||||||:|||.:|||.|:::|.|...||||..:|..
Mosquito   234 ENLNHAFDVAERELGIPQLLDAEDIDTARPDEKSILTYVASYYHTFARMKSEQKGGKRIANIVNK 298

  Fly   293 AMENDKMVHDYENFTSDLLKWIETTIQSLGEREFENSLAGVQGQLAQFSNYRTIEKPPKFVEKGN 357
            .|:.||....:|:..::||.||......|.:|.|.||:.|:||:|..|..|||:|||||:.|:..
Mosquito   299 LMDADKKKMQFEHLITNLLSWIRAKTVELEKRNFPNSVEGIQGELLAFKEYRTVEKPPKYKERSE 363

  Fly   358 LEVLLFTLQSKMRANNQKPYTPKEGKMISDINKAWERLEKAEHERELALREELIRQEKLEQLAAR 422
            :|.|.|.:.:.:::.||..|||::||||:||.|||:|||.|||.||:|||:||:||||||||..:
Mosquito   364 IEALYFHVNTLLKSLNQPHYTPQDGKMINDIEKAWQRLENAEHNREVALRDELLRQEKLEQLNFK 428

  Fly   423 FDRKASMRETWLSENQRLVSQDNFGFDLAAVEAAAKKHEAIETDIFAYEERVQAVVAVCDELESE 487
            |::|:.:||.:|:|..::::...:|..|..|:|..||||||..||.|..:|...:..:|:||..|
Mosquito   429 FEKKSVLREGYLNEMIQVLTDPRYGPALRQVDATVKKHEAISADILARADRFNDLTDMCNELHQE 493

  Fly   488 RYHDVKRILLRKDNVMRLWTYLLELLRARRMRLEISLQLQQNFQEMLYILDNMEEIKQLLMTDDY 552
            .||...|:..|:..|:..|..|||||...:::|.....|....:|:...:..::.::....::|.
Mosquito   494 NYHGKDRVKKRELEVITKWKELLELLENHKLKLSQMSSLMNLLREIDATMTTVKALQAQFASEDV 558

  Fly   553 GKHLMGVEDLLQKHSLVEADINILGERVKVVVQNSQKFLSDDPESYKPCDPEIIVSRVQQLEDAY 617
            |.||:|||:|||.|||:|..:..|||..:..|:..:.|...|   ||  |...:.:::.:|...|
Mosquito   559 GPHLLGVEELLQAHSLLELQVTTLGETQRRYVRQGEAFKRGD---YK--DTSQLDAKLAELAKLY 618

  Fly   618 AELVRLAVERRSRLEESRKLWQFYWDTADEENWIKEKEQIVSTDEVGHDLTTVNLMLSKHKALES 682
            .||:.::..||:||:|:|..:||..|..:||.|:.:|::|........||..|..:..||||||.
Mosquito   619 EELLAMSAARRARLDEARDFYQFVEDHENEEGWLVDKQRICKAGITAKDLRAVMSLQQKHKALED 683

  Fly   683 EITSHDPQLQNVAKVGSELITEGHFGADRIKDRLKEILNKWDHLLDLTKYRRQRLENAVEYFQLF 747
            |:....|:...:.:.|.:||.:.|.....::.::..:...|..|.||...|:::||:|.|.:|.:
Mosquito   684 EMKVRKPKSNQITEAGKKLIVDKHSRMSDVQGKIDSLQEHWKALEDLVDLRKRQLEDAAEAYQYY 748

  Fly   748 ADADDVDNWMLDTLRIVSSEDVGRDEANVQSLLKKHKDVADELKNYAEVIDALHKQAESL----- 807
            |||::.|:|:.:.:.:|:|:|.|.||.:.|:||::|:|:..||..|:..|..|::|||.|
Mosquito   749 ADANEADSWLNEKMALVASDDFGVDEPSAQALLQRHRDLQGELNAYSGDILNLNQQAEKLIKAGI 813

  Fly   808 ----------------------------------------------------------------- 807
                                                                             
Mosquito   814 CTLDLSAEPEPVQEMEQEEWVNETRLVPKEVWEDEPVERLEHRTVTENKLLPHVQALYRFEGQNM 878

  Fly   808 ----------------------KLN------------------------EAEKA----------- 815
                                  ||:                        :|||.
Mosquito   879 KVAKGDVMILQNKNNVDWWNVRKLDGTEGFVPANYVKEIEPRPVPCLVRKAEKVKVMQKVKKTIL 943

  Fly   816 --------------------------------------NVDKRLEAIDNRYKELTELAKLRKQRL 842
                                                  :||||.:.|:..|.:|.:||:.|:..|
Mosquito   944 VKEVIQVKRVRPAKVSQMKPLVKRRAKGDESSAVDSNDSVDKRQKHINGTYDQLQDLAERRRALL 1008

  Fly   843 LDALSLYKLMSEADGVEQWIKEKTKMLDTMTPGKDIEDVEIMKHRFEGFDKEMNANASRVAVVNQ 907
            .|::.|::...|.|..|:|||:|.|||.|..|.   ..||..:.:||.|..:::|::.||..::.
Mosquito  1009 EDSICLFRFYRECDDFEKWIKDKEKMLRTDDPK---GSVETARRQFETFVNDLSASSKRVEAIDS 1070

  Fly   908 LARQLLHVEHPNSDEILERQNHLNQEWSTLREKAEAKMDDLKSAHGVQTFYIECRETISWIEDKK 972
            .....:...|...|::..||..::|.|..|......|...|:.|..|:.||..|.|.|.|:.:|
Mosquito  1071 DVEDFVRQGHSQLDKVKARQRQIHQMWEHLNYLKGQKEKSLEGASSVELFYRTCEEAIDWMNEK- 1134

  Fly   973 RILTETDSLEM--DLTGVMTLQRRLSGMDRDLAAIQAKLSSLEREANSIEDEHPEEAKIIRERIA 1035
              :|:.|:.|:  ||..|..||||...::|:||.::.|:|.:....|::::.:|.|...:.|:..
Mosquito  1135 --MTQLDTAEVGPDLKTVQALQRRHENLERELAPVKEKVSRVNLLGNTVKNSYPSERDNVSEKQR 1197

  Fly  1036 QIELIWEQLTQMLKERDSKLEEAGDLHRFLRDLDHFQTWLTKTQTDVASEDTPTSLPEAEKLLNQ 1100
            .|:.:|:::.:..|||.|:||.|.....|.........|:.:....:|:|:|...:..|||||.:
Mosquito  1198 DIQNLWQKVQEKAKERRSRLENAVGQQIFNNSTKALLNWIAECNNQLAAEETARDVETAEKLLKK 1262

  Fly  1101 HQSIREEIDNYTEDYKNMMEYGERLTSEG-STSDDPQYMFLRERLNALKDGWEELHQMWENRQVL 1164
            |:.:.|:|:.:.:::..:.:.|:::.... :.::|.:   :..::..|:...:.||..|..::..
Mosquito  1263 HKDLGEDINAHDDEFVQLAKLGQQIIERNPALTEDHE---MANKIAKLEAERQRLHGAWLAKEKK 1324

  Fly  1165 LSQSLDQQLFNRDARQTEVLLSQQEHFLSKDDTPVNLEQAENQLKRHEAFLTTMEANDDKINTLL 1229
            |.|.::.|:|||:|.:.:......|.:|..||...:|:..|..:|||..|..::.|.|..:....
Mosquito  1325 LQQCIELQIFNREADKIDATTKSHEAYLEYDDLGNSLDDVEAIMKRHIDFENSLGAQDKILKNFS 1389

  Fly  1230 QVADTLVEKDHFDADKIGKRAENITGRRDDNRQRALDQHEKLKNQVKLHEFLQDLEELAEWVQEK 1294
            :.||.|:..:|::|..|..|...:..||:..::.|..:...|:......:|..|:::|..|:.:|
Mosquito  1390 EGADRLIRNNHYNATYIDDRRGQVLARRERVKELAQKRRNALQASKDFQKFSSDVDDLNRWLADK 1454

  Fly  1295 YATSQDESYRSAKTIHSKWTRHQAFEAEIAANKERLFEAEKSAQELSKEKPEFKDVIEPKLKELA 1359
            ...:.||||:....:..|..:|:|||.|:.||:.:|....|..:.|.|......:|.| .|..:.
Mosquito  1455 TKIASDESYKDLSNLPRKLQKHKAFERELRANEGQLRMVNKEGESLIKTNNRAGEVSE-TLAAIN 1518

  Fly  1360 KQFDDLEVHTKEKGAMLFDANREVLVQQTCDDIDSYITDLEKQIVSGDTANDLTSVNILMQKQQV 1424
            :.:.||...:.:||..|..|..:....:..:|..|...:|:..:.|....|||.|...||.|.||
Mosquito  1519 QNWKDLVATSLDKGRRLEQAALQREHNRYIEDSKSKFEELDHALQSKQVGNDLRSCKELMNKHQV 1583

  Fly  1425 IQTQMAVKARQV-------EEIDKQTEYLQKTVPEEKIEPIVVKKTAVLERFEKIKAPLLERQKA 1482
            ::..:|:..::|       ||:..:..:....:..|      .||  :..:|..:|.|..:|:.|
Mosquito  1584 LENDIALWEQKVAELVTTGEEMAHEGHFDANNIQNE------TKK--LQAQFAGLKTPARQRRDA 1640

  Fly  1483 LEKKKEAFQFCRDVEDEKLWIDEKLPVANSPDYGNSLFNVHVLKKKNQSLATEIDNHEPRINAIC 1547
            |::.....:|..:::.|..||:|.||.|.|...|.:|.....|.||::.|..||:.|:|.||...
Mosquito  1641 LDESLRFHKFVFELDTELQWINEHLPAAKSEVIGQNLHQAQSLSKKHKKLEAEIEGHQPMINKTL 1705

  Fly  1548 NNGRKLIDEGHEDAKKFEALISDLTQKWQELKDAIENRRKHLLESEKVQQYFFDAQEAESWMSEQ 1612
            .:...||::.|.:..|.:.|.:.|.:.|.:|::....|.:.|..|.|.|||..||.|.|:|:.|:
Mosquito  1706 TSAENLINQNHPERAKVKELCNSLEKAWADLQEQSAERSRKLELSLKAQQYLSDAGEIETWLGER 1770

  Fly  1613 ELYMMVEDRGKDEISAQNLMKKHENLEQSVEDYANTIRQLGEVARQFSGDDISSGDAVAVKQSQL 1677
            ...:...|.|:|..||..|:.||:.:|..::.|:..:.::|..|.............:|.||..:
Mosquito  1771 NNVLRSSDYGRDRDSATKLLTKHKVIELELDTYSGIVSEMGHTASAMIAAKHPDSKVIAAKQQLI 1835

  Fly  1678 DKLYAGLKDLAGERRARLNEALQLFMLSREVDDLEQWITDREVVAGSQELGQDFDHVTLLSERFN 1742
            :|:...|:.|||:|:.||.|:|.......|..:|||||.::|....|::.|.|::|:.:|..:|:
Mosquito  1836 EKMLKSLQRLAGQRQLRLMESLYRHEYFVESAELEQWIKEQEQAVNSEDYGHDYEHLLILQNKFD 1900

  Fly  1743 EFARDTEAVGGERVAKVNGIADNLIQAGHSDSATIAEWK--------------------DNLNES 1787
            :.....| ||.||..:....|..||   ..||..::|.:                    |||..:
Mosquito  1901 DLKHRIE-VGAERFNQCENFAKKLI---GGDSPYVSEIEKRQELLRSFSEAQLDVVDMYDNLRNA 1961

  Fly  1788 WQDLLELIETRTQMLAASRELHKFFHDCKDVLGRILEKQHGVSDELGRDAGSVSTLQRKHYNFLQ 1852
            ||.|||.::.|.:.|.|:.|:|:|..|..:.|.||.:|...:|.|||:|..|...|.|||..|..
Mosquito  1962 WQHLLEQLDAREKKLHAAGEIHRFHRDAAEALFRIQDKNAALSSELGKDLNSALALARKHEAFEN 2026

  Fly  1853 DLITLYSQVQQIQEESAKLQDAYAGDKAKEITNREQEVLHAWDNLQAMCDARKQKLADTGDLFRF 1917
            :|:.|.:|:|.:.::|.:||..|.||.||.|...::.|:..|:.|:.....|..:|..:.||..|
Mosquito  2027 ELVPLEAQLQVLVDDSLRLQTKYPGDNAKAIAAEQENVIQYWNVLKEKSALRNDQLHASCDLQHF 2091

  Fly  1918 FNMVRILMIWMEDLVRQMNTSEKPRDVSGVELLMNNHQSLKAEIDTREDNFGACISLGKELLTRN 1982
            ...||.||.|..:|...:...|...|.:|...|...|.::..||:.||..|.....|...::...
Mosquito  2092 LTQVRDLMSWATNLRAALQAEEHVSDAAGATALKIQHDAIYGEIEAREQTFRELNELSDSMVQTG 2156

  Fly  1983 HYASADIKDRLMTLSNSRNALLRRWEERWENLQLILEVYQFARDAAVAEAWLIAQEPYLLSSELG 2047
            |||:.:::::...|.:.|..|...:.::...|:..::::.|.|||.|.:.....||..|.||:.|
Mosquito  2157 HYAATEVEEKCSALLDERAKLHSAYNKKKILLEQKIDLFCFMRDAKVIDNISSGQEATLSSSDFG 2221

  Fly  2048 HTIDEVENLIKKHEAFEKSAAAQEERFSALERLTTFELKEMKRRQELAEEAERQRIKE--EQEAK 2110
            .|::.|::.:|:||||||....|:|:.:.|:.    ..:::..:.....|..|:|::|  |:..|
Mosquito  2222 QTVEVVQDQVKRHEAFEKLIQTQDEKVAILQE----HGRKLVEQNHYDSENIRRRLREIVERRQK 2282

  Fly  2111 AASEAAEQAKREAER-------RDDVDVGASHDDSAAKDTAVEFERVVEIQTERGGTPGAGEGHE 2168
            .....|.:.::.|..       ||..:.||..::...|..|                    :.|
Mosquito  2283 VKDLCALRRQKLANALLYAQFIRDCAEAGAWINEKQKKLEA--------------------DAH- 2326

  Fly  2169 GYVTRKHEWDSTTKKASNRSWDKVYMAAKAGRISFYKDQKGYKSNPELTFRGEPSYDLQNAAIEI 2233
            .|....:..|...|....:::| ..:.|..|||...:| ||.|.   :..:.|.:.::|:..:::
Mosquito  2327 SYAGASNMEDKVKKLKKLQAFD-AEVNANEGRIREVRD-KGEKL---IAKKHECAGEIQDELVKL 2386

  Fly  2234 ASDYTKKKHVLRVKLANGALFLLQAHDDTEMSQWVTSLKA 2273
            ...:    :||..:||:.:..|.:|.|..|.:..:..|:|
Mosquito  2387 MQSW----NVLLNELASRSRGLKEAQDILEFNTQLDKLEA 2422

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
beta-Spec	NP_001259660.1	CH_SPTB-like_rpt1	36..152	CDD:409095	66/115 (57%)
		CH_SPTB_like_rpt2	169..273	CDD:409097	65/103 (63%)
		Spectrin	298..408	CDD:395348	52/109 (48%)
		SPEC	422..521	CDD:197544	35/98 (36%)
		SPEC	525..740	CDD:238103	65/214 (30%)
		SPEC	639..846	CDD:238103	75/371 (20%)
		SPEC	848..1058	CDD:238103	66/211 (31%)
		SPEC	955..1169	CDD:238103	56/216 (26%)
		SPEC	1170..1380	CDD:238103	57/209 (27%)
		SPEC	1386..1484	CDD:197544	25/104 (24%)
		SPEC	1488..1699	CDD:238103	66/210 (31%)
		SPEC	1594..1806	CDD:238103	70/231 (30%)
		SPEC	1807..2015	CDD:238103	63/207 (30%)
		Spectrin	2018..>2078	CDD:395348	22/59 (37%)
		PH_beta_spectrin	2167..2274	CDD:269975	25/107 (23%)
kst	XP_061506980.1	None