DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGAPp190 and arhgap35b

DIOPT Version :9

Sequence 1:	NP_001259658.1	Gene:	RhoGAPp190 / 32743	FlyBaseID:	FBgn0026375	Length:	1621	Species:	Drosophila melanogaster
Sequence 2:	XP_003198691.2	Gene:	arhgap35b / 564565	ZFINID:	ZDB-GENE-060526-44	Length:	1517	Species:	Danio rerio

Alignment Length:	1684	Identity:	478/1684 - (28%)
Similarity:	801/1684 - (47%)	Gaps:	321/1684 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 FNISVIGLSGTEKDRGQVGVGKSCLCNRFMRPMADDYFIDHISVLSQSDFSGRIVNNDHFLYWGD 68
            :|:.|:|||||||::||.|||||||||||:||.|||:.:||.||||.|||.||:|||||||:||:
Zfish    14 YNLVVVGLSGTEKEKGQCGVGKSCLCNRFVRPSADDFHLDHTSVLSTSDFGGRVVNNDHFLFWGE 78

  Fly    69 VRKTTEEGVEYQFNIIEQTEFMDDSTFQAFKVGKMDPYSKRCTATKVFSAEKLMYICKNQLGIEK 133
            |.:..|:|.:.:.:::|||||:||.|||..:...:.||.||..:||:.|||||||.|.:|||:|:
Zfish    79 VGRVLEDGADCRMHVVEQTEFIDDQTFQPHRSTALLPYIKRAASTKLASAEKLMYFCTDQLGLEQ 143

  Fly   134 EYEQKVMPDGRLSIDGFVVVFDVSPVPNRSVEKQVEFVQNVIATILKNKKPLVLVTTKNDDAYEL 198
            ::|||.||:|:|.:|||::..|||...||:.:.|::||.|:...:.|.|||:|||.||.|:..|.
Zfish   144 DFEQKQMPEGKLQVDGFLLCVDVSRGMNRNFDDQMKFVTNLYNHLSKTKKPIVLVLTKCDEGVER 208

  Fly   199 YVREAEKISQRKDYKSTVQLIETSAHESINIDLAFLLLAQMIDKVKNRVKIISYQESAKSRKELL 263
            |::::...:..   |..:|::||||..:||:|||||.|.|:|||.|.:.|||.|.|:.|.:.:|:
Zfish   209 YIKDSHTFAIT---KKNLQVVETSARSNINVDLAFLTLIQLIDKGKGKPKIIPYFEALKHQSQLI 270

  Fly   264 DTRSEAVTRLIRNQITDYHVLWSQGSKMLSQYREWNEFLNIFGHEAGQKLFRRHMKKLRDDHLNK 328
            .:..:....|:.:.:.:::..|...|:.:....|:.|::.:.|....:|||::|:.:|:.:|..:
Zfish   271 ASAKDRYEWLVNHIVKNHNETWPNVSRRMQTSPEYKEYVFLEGTGKAKKLFQQHIHRLKQEHTER 335

  Fly   329 KLHQYLDKFALALEYLLPDIGALNISDDDAWECARNYLQNHIEFEQYFFECPQASWTELVDMDEA 393
            :...||....|||..|:.::..:   :..:|...:..|::..:|:.:|.....|.|.:...:|..
Zfish   336 RRKAYLSALPLALSSLVSELDEI---EHLSWSGVQKVLESKKDFDHWFVVLDDAPWEDTPHLDNM 397

  Fly   394 EDEARIPFDVLETSEAETVFRNYLNSVQQDKKKIGWKQQFKMLLEESGFVTPGKQLSEVRVLFMG 458
            ||: |||||||:|:.||.:|..:|..::.:.|:...:.:||..|..|.||||||...|.|...|.
Zfish   398 EDD-RIPFDVLDTTAAEILFDAHLEHLRNECKRAQMRHEFKGKLASSPFVTPGKPWEEARSFIMN 461

  Fly   459 RECFEALSEHDCQQIYDIHQDDIIEKSKQNFVELLLEHAQYFLQFKNVDNITQEDVRQITDVIQE 523
            .:.::.|.|.:...||:.||..||:::|::|.|||||:::.|.:.:.....::|.:..|.:|:.|
Zfish   462 EDFYQWLEEPEYLDIYNRHQKAIIDRAKEDFQELLLEYSELFYELEVDAKPSKEKMGAIQEVLGE 526

  Fly   524 DSRYKMLDRLDQERRLMLVQHLRFIHCPIRDHCPFFYNCVDSLIEEVLSDKSASNHKTPSGGGWK 588
            :.|:|.|.:|..||..::::|:.|::.|.::.||...:||||.||::|:  |....:.||.|...
Zfish   527 EQRFKALQKLQAERDALVLKHIHFVYHPTKETCPNCPHCVDSKIEQILA--SCFPAQYPSFGKSH 589

  Fly   589 SSGSGSDRTLNLLIVGSEHLASDLLNDIR-ICTGSKGEYIYENQTYYLNYRIANGD----MEAFK 648
            .:.:..|| :||:|:|.:.||.:|.|:|| :|| :...|:.|.:.|.|..|...|:    :.:|.
Zfish   590 ITDAKVDR-INLVILGKDGLARELANEIRALCT-NDDHYVLEGRMYELVLRPIEGNVRLPVNSFH 652

  Fly   649 AIDVYSSGLICVYSNQQSFETLKDNLERTLLCNLELEDK-FENLPIVLVYQPQ-------DLKEN 705
            .......|.:|:|::::|...:.:::|:.....|...|. ...||:.|:...:       |:...
Zfish   653 TSTFTPHGCLCLYNSKESLSYVVESIEKLRESTLGRRDNHMAQLPLSLLLVSKRGVGGLSDIGGE 717

  Fly   706 EVEYLRNEGMRLSEMLHCDFID-------HTQN-HQKYVYDILNIVILSLKLTEMKSYE----PY 758
            ..:.|.::|.:::..|.|::::       :.:| ::|.:..:|..::.:.:.:..:|..    |.
Zfish   718 TAQALISQGQQVAIKLQCNYLEPSSPGTGYGRNVNEKQINQVLKALLETRRTSTFRSCSPPAPPL 782

  Fly   759 P----------SNHTDLRILCCIFCGDQYDIENIVQPLVE----ESTLVKANEHSIIVDVFIGDA 809
            |          |...||||:.|:.|||.|||:.::.|.:.    :.|..:.:..|::::..||..
Zfish   783 PPCIRDFQQDQSPEADLRIVMCLMCGDSYDIDQLLAPFLLPQHCKPTSPQTSGTSVLLEQTIGGH 847

  Fly   810 KRRVEFILSSYHGT-SQYRDELIHGYIYFYSTKRRSSLANL-SILAAQNANIPLQIIAVTESGGV 872
            |..:|..|.|||.: :..:..|:||||..||..|::|...| :.|......||:|:|||.|:  .
Zfish   848 KLTIELSLLSYHSSFATRKSRLVHGYIAVYSASRKASFETLCAFLCEVQDIIPVQMIAVGET--Q 910

  Fly   873 NAFFNSDICQFLITEGNAVADRFKGSFMTFSADQ------YVKFAFYNPFLKTAWDNKYEVENLH 931
            ....:|:..:.|:.:|..:|...:|.|.:.....      ..|......|.....:.|..||..|
Zfish   911 IELQDSESARELLVQGEELAHELEGRFGSIVCGHGGVVGGLHKLDLLEQFFTEVLEKKTIVEASH 975

  Fly   932 VEESITLDSGEGTLEN-----------------SVNQMPRPPPRHESYMLSNTLGTDGSGSENYE 979
            :.||:.  ....|.||                 |...|...||.|:     .||.:.|    .::
Zfish   976 MYESMA--EASSTNENVYSPHCGSPSPVTMLLDSEEDMEASPPYHD-----GTLTSHG----GFK 1029

  Fly   980 MAPTRSLNSLNEERDISLDEIYDDNEKPKHLHQRFQIFPPPTTPPEPAPPDHQLITCTYKSQFVS 1044
            :....|.::.:...|:|..|...:|:            .||...|:|:
Zfish  1030 LPDLDSGDTFSVISDLSTFENKLNNK------------VPPQVRPKPS----------------- 1065

  Fly  1045 ASESSLEEITSDVSGSKDSLATHDAEWLEDKSDGRRNMNKNLIWNNFSGSTHAYTTGRRHIDSNL 1109
                    :|.|:  .|.|...:     .|....||::..|:.|
Zfish  1066 --------VTFDL--RKPSFNPY-----VDGVSHRRSLTSNVTW--------------------- 1094

  Fly  1110 NKIRPKG----PSQTLKVGEAPSRNCPAMSSSTFTLP---TQQPGK-LNMKNFQLVSDAVAKMNF 1166
                |.|    ||...:..:|.|:..|:...|.:::|   ||  || :.::|...:
Zfish  1095 ----PLGGDYDPSDYAEPMDAVSKPRPSYEDSIYSVPHDSTQ--GKIITIRNSNRI--------- 1144

  Fly  1167 TGSGSGSGSGSGSGSTGLGLGLGSGSGCMGDSFLEPVDKDGKRYDHAQLDGEDEDSEELAEYEQI 1231
                ..:|.|:||.|.|                                ||
Zfish  1145 ----HSNGGGNGSDSEG--------------------------------DG-------------- 1159

  Fly  1232 YENEDCTESDSCASSTERRVRQQNAYYKASKKPVAAKKQKKKKVAIPVQTPRVPPFGSYVSPPEI 1296
                         ||.|||    ..:..|..||...:.:.|          |:..|.|:.:
Zfish  1160 -------------SSLERR----RKFSAARVKPRLYRDRSK----------RLGKFSSFRT---- 1193

  Fly  1297 PLHYQRMAVGGS---GPGEKKKPEPCVPEFMKSDKSPEYSMVPELAGAGIFGAENLPEYNMNQAK 1358
                 ..::|..   |...:.|.:...|  :|:|.:.|
Zfish  1194 -----SFSIGSDDEIGTLSRSKEDDVSP--LKTDITNE--------------------------- 1224

  Fly  1359 CLKDFEKLEKRRIKEETARQRKLQEKEKEQEKKLKRKLKQNAKGLVESAEAQFGKLMITSEQGEI 1423
                         :.|..::|.:.:..:...||.:.|.:.:....:||.......:.:.|.:..|
Zfish  1225 -------------EGEDPKKRNILKSLRRPGKKARPKPRHSISKPLESNYFGVPLVNVVSFERPI 1276

  Fly  1424 PIFLNKCVEFIEKEGLDSEGIYRVPGSRAHVDMLFQRFEEDTNTEIDALDIPVNAVATALKDFFS 1488
            |:|::||:.:||..||.:||||||.|::|.::.:.::||:|.|.:....|..||.||.|:|.|||
Zfish  1277 PVFIDKCIRYIEATGLTTEGIYRVSGNKAEIEGMQRQFEQDHNLDFVEKDFTVNTVAGAMKSFFS 1341

  Fly  1489 KRLPPLFSKDIIKELEEIAGSRGVGNSKLNVEVKTDRSCRLIALKSLLQKLPPINFAILKYIFQH 1553
            :...||......:||.|.        .|:|     ||..||..:|.:|::.|..||.:.||:..|
Zfish  1342 ELPDPLVPYSSQEELVEA--------FKIN-----DREQRLHTMKDVLRRFPRENFDVFKYVMSH 1393

  Fly  1554 FVHVSDNSKLNSMDSKNLAICWWPTLIPIDFTDMGHFEQLRPYLEDIVQTMIDQFPYLF 1612
            ...|...:::|.|.|:||:||:||||:..|||.|......|.| :.|::|.|.|..:.|
Zfish  1394 LNKVGQWNRVNLMTSENLSICFWPTLMRPDFTSMDALTATRTY-QTIIETFIHQCAFFF 1451

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGAPp190	NP_001259658.1	P-loop_NTPase	<148..238	CDD:304359	38/89 (43%)
		RhoGAP-FF1	254..333	CDD:293121	15/78 (19%)
		RhoGAP_p190	1411..1611	CDD:239838	73/199 (37%)
arhgap35b	XP_003198691.2	Ras_like_GTPase	29..245	CDD:206648	114/218 (52%)
		Ras	<158..247	CDD:331851	39/91 (43%)
		RhoGAP-FF1	261..340	CDD:318668	15/78 (19%)
		FF	431..483	CDD:128718	19/51 (37%)
		RhoGAP_p190	1262..1446	CDD:239838	72/197 (37%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	197	1.000	Domainoid score	I3056
eggNOG	1	0.900	-	-		E1_KOG4271
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H35136
Inparanoid	1	1.050	711	1.000	Inparanoid score	I629
OMA	1	1.010	-	-		QHG50036
OrthoDB	1	1.010	-	-		D110157at2759
OrthoFinder	1	1.000	-	-		FOG0003137
OrthoInspector	1	1.000	-	-		otm24424
orthoMCL	1	0.900	-	-		OOG6_105533
Panther	1	1.100	-	-	O	PTHR46005
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4737
SonicParanoid	1	1.000	-	-		X2106
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	15	14.870