DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGAPp190 and Arhgap35

DIOPT Version :9

Sequence 1:	NP_001259658.1	Gene:	RhoGAPp190 / 32743	FlyBaseID:	FBgn0026375	Length:	1621	Species:	Drosophila melanogaster
Sequence 2:	NP_766327.3	Gene:	Arhgap35 / 232906	MGIID:	1929494	Length:	1499	Species:	Mus musculus

Alignment Length:	1672	Identity:	496/1672 - (29%)
Similarity:	825/1672 - (49%)	Gaps:	312/1672 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 FNISVIGLSGTEKDRGQVGVGKSCLCNRFMRPMADDYFIDHISVLSQSDFSGRIVNNDHFLYWGD 68
            :||||:|||||||::||.|:|||||||||:||.||::.:||.||||.|||.||:||||||||||:
Mouse    14 YNISVVGLSGTEKEKGQCGIGKSCLCNRFVRPSADEFHLDHTSVLSTSDFGGRVVNNDHFLYWGE 78

  Fly    69 VRKTTEEGVEYQFNIIEQTEFMDDSTFQAFKVGKMDPYSKRCTATKVFSAEKLMYICKNQLGIEK 133
            |.::.|:.||.:.:|:|||||:||.|||..:...:.||.||..|||:.|||||||.|.:|||:|:
Mouse    79 VSRSLEDCVECKMHIVEQTEFIDDQTFQPHRSTALQPYIKRAAATKLASAEKLMYFCTDQLGLEQ 143

  Fly   134 EYEQKVMPDGRLSIDGFVVVFDVSPVPNRSVEKQVEFVQNVIATILKNKKPLVLVTTKNDDAYEL 198
            ::|||.||||:|.:|||::..|||...||:.:.|::||.|:...:.|.|||:|:|.||.|:..|.
Mouse   144 DFEQKQMPDGKLLVDGFLLGIDVSRGMNRNFDDQLKFVSNLYNQLAKTKKPIVVVLTKCDEGVER 208

  Fly   199 YVREAEKISQRKDYKSTVQLIETSAHESINIDLAFLLLAQMIDKVKNRVKIISYQESAKSRKELL 263
            |:|:|...:..   |..:|::||||..::|:||||..|.|:|||.:.:.|||.|.|:.|.:.:.:
Mouse   209 YIRDAHTFALS---KKNLQVVETSARSNVNVDLAFSTLVQLIDKSRGKTKIIPYFEALKQQSQQI 270

  Fly   264 DTRSEAVTRLIRNQITDYHVLWSQGSKMLSQYREWNEFLNIFGHEAGQKLFRRHMKKLRDDHLNK 328
            .|..:....|:...:.:::..|...|:.:....|:.:::.:.|.:..:|||.:|:.:|:.:|:.:
Mouse   271 ATAKDKYEWLVSRIVKNHNENWPSVSRKMQASPEYQDYVYLEGTQKAKKLFLQHIHRLKHEHIER 335

  Fly   329 KLHQYLDKFALALEYLLPDIGALNISDDDAWEC--ARNYLQNHIEFEQYFFECPQASWTELVDMD 391
            :...||....||.|.|:|     |:.:.|...|  |:..|:...||.::|....:..|.....:|
Mouse   336 RRKLYLAALPLAFEALIP-----NLDEVDHLSCIKAKKLLETKPEFLKWFVVLEETPWDATSHID 395

  Fly   392 EAEDEARIPFDVLETSEAETVFRNYLNSVQQDKKKIGWKQQFKMLLEESGFVTPGKQLSEVRVLF 456
            ..|:| |||||:::|..||.::..:|..::.::|:...::.||..||.|.|:||||...|.|...
Mouse   396 NMENE-RIPFDLMDTVPAEQLYETHLEKLRNERKRAEMRRAFKENLETSPFITPGKPWEEARSFI 459

  Fly   457 MGRECFEALSEHDCQQIYDIHQDDIIEKSKQNFVELLLEHAQYFLQFKNVDNITQEDVRQITDVI 521
            |..:.::.|.|.....||..||..||:::|:.|.|||||:::.|.:.:.....::|.:..|.||:
Mouse   460 MNEDFYQWLEESVYMDIYGKHQKQIIDRAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVL 524

  Fly   522 QEDSRYKMLDRLDQERRLMLVQHLRFIHCPIRDHCPFFYNCVDSLIEEVLSDKSASNHKTPSGGG 586
            .|:.|:|.|.:|..||..::::|:.|::.|.::.||....|||:.||.::|    |....||...
Mouse   525 GEEQRFKALQKLQAERDALILKHIHFVYHPTKETCPSCPACVDAKIEHLIS----SRFIRPSDRN 585

  Fly   587 WKSSGS--GSDRTLNLLIVGSEHLASDLLNDIR-ICTGSKGEYIYENQTYYLNYRIANGD----M 644
            .|:|.|  ..|| :||:|:|.:.||.:|.|:|| :|| :..:|:.:.:.|.|:.|...|:    :
Mouse   586 QKNSLSDLNIDR-INLVILGKDGLARELANEIRALCT-NDDKYVIDGKMYELSLRPIEGNVRLPV 648

  Fly   645 EAFKAIDVYSSGLICVYSNQQSFETLKDNLERTLLCNLELEDK-FENLP--IVLVYQPQDLKENE 706
            .:|:.......|.:|:|::::|...:.:::|::....|...|. ..:||  ::||.:..|.....
Mouse   649 NSFQTPTFQPHGCLCLYNSKESLSYVVESIEKSRESTLGRRDNHLVHLPLTLILVNKRGDTSGET 713

  Fly   707 VEYLRNEGMRLSEMLHCDFID-------HTQN-HQKYVYDI----------LNIVILSLKLTEMK 753
            :..|..:|.:::..|.|.|:|       :.:| ::|.:..:          ||:|..:..:.::.
Mouse   714 LHSLIQQGQQIASKLQCVFLDPASAGIGYGRNINEKQISQVLKGLLDSKRNLNLVSSTASIKDLA 778

  Fly   754 SYEPYPSNHTDLRILCCIFCGDQYDIENIVQPLVEESTLVKA---NEHSIIVDVFIGDAKRRVEF 815
            .        .||||:.|:.|||.:..::::.|:::..|...:   :.:|:::::.||..|:|:|.
Mouse   779 D--------VDLRIVMCLMCGDPFSADDVLSPVLQSQTCKSSHCGSSNSVLLELPIGLHKKRIEL 835

  Fly   816 ILSSYHGTSQYR-DELIHGYIYFYSTKRRSSLANL-SILAAQNANIPLQIIAVTESGGVNAFFNS 878
            .:.|||.:...| ..|:||||.|||.||::|||.| :.|......||:|::|:|: |.::...|
Mouse   836 SVLSYHSSFSIRKSRLVHGYIVFYSAKRKASLAMLRAFLCEVQDIIPIQLVALTD-GAIDVLDN- 898

  Fly   879 DICQFLITEGNAVADRFKGSFMTFSADQ-YVKFAFYNPFLKTAWDNKYEVENLHVEESITLDSGE 942
            |:.:..:|||..:|....|.|.:....| ..|...::||.|...:.|..:|..|:.:::.  ...
Mouse   899 DLSREQLTEGEEIAQEIDGRFTSIPCSQPQHKLELFHPFFKDVVEKKNIIEATHMYDNVA--EAC 961

  Fly   943 GTLENSVNQMPRPPPRHESYMLSNTLGTDGSGSE--NYEM-APTRSLNSLNEERDISLDEIYDDN 1004
            .|.|...|.     ||..|.:.::.|.......|  :|.: ....:|.||:::......|: :.|
Mouse   962 STTEEVFNS-----PRAGSPLCNSNLQDSEEDVEPPSYHLFREDATLPSLSKDHSKFSMEL-EGN 1020

  Fly  1005 EKPKHLHQRFQI-----FPPPTTPPEPAPPDHQLITCTYKSQFVSASESSLEEITSDVS------ 1058
            :....:...|:.     .|||.   :|.||.|                   .:||.|:|
Mouse  1021 DGLSFIMSNFESKLNNKVPPPV---KPKPPVH-------------------FDITKDLSYLDQGH 1063

  Fly  1059 --GSKDSLATHDAEWLEDKSDGRRNMNKNLIWNNFSGSTHAYTTGRRHIDSNLNKIRPKGPSQTL 1121
              |.:.|:::  :.|:  ..||            |..|.:|     ..:|:   .::|:...:.:
Mouse  1064 REGQRKSMSS--SPWM--PQDG------------FDPSDYA-----EPMDA---VVKPRNEEENI 1104

  Fly  1122 KVGEAPSRNCPAMSSSTFTLP---TQQPGK-LNMKNFQLVSDAVAKMNFTGSGSGSGSGSGSGST 1182
                             :::|   ||  || :.::|       :.|....|||:||.|
Mouse  1105 -----------------YSVPHDSTQ--GKIITIRN-------INKAQSNGSGNGSDS------- 1136

  Fly  1183 GLGLGLGSGSGCMGDSFLEPVDKDGKRYDHAQLDGEDEDSEELAEYEQIYENEDCTESDSCASST 1247
                                                                    |.|:.:...
Mouse  1137 --------------------------------------------------------EMDTSSLER 1145

  Fly  1248 ERRVRQQNAYYKASKKPVAAKKQKKKKVAIPVQTPRVPPFGSYVSPPEIPLHYQRMAVGGS---G 1309
            .|:|       .|..|||..:          .:..|:..|.||.:         ..:||..   |
Mouse  1146 GRKV-------SAVSKPVLYR----------TRCTRLGRFASYRT---------SFSVGSDDELG 1184

  Fly  1310 PGEKKKPEPCVPEFMKSDKSPEYSMVPELAGAGIFGAENLPEYNMNQAKCLKDFEKLEKRRIKEE 1374
            | .:||.|....:..|.|.    :::|                          :|       .:|
Mouse  1185 P-IRKKEEDQASQGYKGDN----AVIP--------------------------YE-------TDE 1211

  Fly  1375 TARQRKLQEKEKEQEKKLKRKLKQN-AKGLVESAEAQFG---KLMITSEQGEIPIFLNKCVEFIE 1435
            ..|:|.:....:...||.|.|.:.: .|...||  ..||   ..::|.|: .||||:.:|:|:||
Mouse  1212 DPRRRNILRSLRRNTKKPKPKPRPSITKATWES--NYFGVPLTTVVTPEK-PIPIFIERCIEYIE 1273

  Fly  1436 KEGLDSEGIYRVPGSRAHVDMLFQRFEEDTNTEIDALDIPVNAVATALKDFFSKRLPPLFSKDII 1500
            ..||.:||||||.|:::.::.|.::|::|.|.::...|..||.||.|:|.|||:...||....:.
Mouse  1274 ATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGAMKSFFSELPDPLVPYSMQ 1338

  Fly  1501 KELEEIAGSRGVGNSKLNVEVKTDRSCRLIALKSLLQKLPPINFAILKYIFQHFVHVSDNSKLNS 1565
            .:|.|.        .|:|     ||..:|.|||.:|:|.|..|..:.||:..|...||.|:|:|.
Mouse  1339 IDLVEA--------HKIN-----DREQKLHALKEVLKKFPKENHEVFKYVISHLNKVSHNNKVNL 1390

  Fly  1566 MDSKNLAICWWPTLIPIDFTDMGHFEQLRPYLEDIVQTMIDQFPYLF 1612
            |.|:||:||:||||:..||:.|......|.| :.|::..|.|.|:.|
Mouse  1391 MTSENLSICFWPTLMRPDFSSMDALTATRSY-QTIIELFIQQCPFFF 1436

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGAPp190	NP_001259658.1	P-loop_NTPase	<148..238	CDD:304359	37/89 (42%)
		RhoGAP-FF1	254..333	CDD:293121	14/78 (18%)
		RhoGAP_p190	1411..1611	CDD:239838	78/202 (39%)
Arhgap35	NP_766327.3	Has GTPase activity, required for proper localization. /evidence=ECO:0000269\|PubMed:11044403	1..266		137/254 (54%)
		RAS	<158..250	CDD:214541	40/94 (43%)
		RhoGAP-FF1	261..340	CDD:406821	14/78 (18%)
		FF	371..422	CDD:128718	16/51 (31%)
		FF	431..483	CDD:128718	19/51 (37%)
		FF	486..539	CDD:128718	17/52 (33%)
		pseudoGTPaseD_p190RhoGAP-A	594..765	CDD:412066	40/172 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1124..1148		9/86 (10%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1177..1207		10/60 (17%)
		Required for phospholipid binding and regulation of the substrate preference. /evidence=ECO:0000250\|UniProtKB:Q9NRY4	1213..1236		6/22 (27%)
		RhoGAP_p190	1247..1431	CDD:239838	76/198 (38%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1446..1499
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	203	1.000	Domainoid score	I2954
eggNOG	1	0.900	-	-		E1_KOG4271
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H35136
Inparanoid	1	1.050	732	1.000	Inparanoid score	I610
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG50036
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0003137
OrthoInspector	1	1.000	-	-		otm43027
orthoMCL	1	0.900	-	-		OOG6_105533
Panther	1	1.100	-	-	LDO	PTHR46005
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4737
SonicParanoid	1	1.000	-	-		X2106
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	14	13.860