DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGAPp190 and arhgap35

DIOPT Version :9

Sequence 1:	NP_001259658.1	Gene:	RhoGAPp190 / 32743	FlyBaseID:	FBgn0026375	Length:	1621	Species:	Drosophila melanogaster
Sequence 2:	XP_004917008.1	Gene:	arhgap35 / 100498460	XenbaseID:	XB-GENE-999477	Length:	1472	Species:	Xenopus tropicalis

Alignment Length:	1670	Identity:	500/1670 - (29%)
Similarity:	800/1670 - (47%)	Gaps:	319/1670 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 FNISVIGLSGTEKDRGQVGVGKSCLCNRFMRPMADDYFIDHISVLSQSDFSGRIVNNDHFLYWGD 68
            :::||:|||||||::||.|||||||||||:||.|||:.:||.||||.|||.||:||||||||||:
Frog    14 YSVSVVGLSGTEKEKGQCGVGKSCLCNRFVRPSADDFHLDHTSVLSTSDFGGRVVNNDHFLYWGE 78

  Fly    69 VRKTTEEGVEYQFNIIEQTEFMDDSTFQAFKVGKMDPYSKRCTATKVFSAEKLMYICKNQLGIEK 133
            ..:..|:.|:::.:::|||||:||.|||..:...:.||.||..:.|:.|||||||.|.:|||:|:
Frog    79 SGRMPEDCVDFKVHVVEQTEFIDDQTFQPHRSTALQPYIKRAASCKLASAEKLMYFCTDQLGLEQ 143

  Fly   134 EYEQKVMPDGRLSIDGFVVVFDVSPVPNRSVEKQVEFVQNVIATILKNKKPLVLVTTKNDDAYEL 198
            ::|||.||:|:|.||||::..|||...||:.:.|::||.|:...:.|.|||:|||.||.|:..|.
Frog   144 DFEQKQMPEGKLLIDGFLLCIDVSRGMNRNFDDQLKFVSNLYTQLAKTKKPIVLVLTKCDEGVER 208

  Fly   199 YVREAEKISQRKDYKSTVQLIETSAHESINIDLAFLLLAQMIDKVKNRVKIISYQESAKSRKELL 263
            |:|:|...:..   |..:|::||||..::|:||||..|.|:|||.:.:.|||.|.|:.|.:.:|:
Frog   209 YIRDAHSFALN---KKNLQVVETSARSNVNVDLAFTTLVQLIDKSRGKSKIIPYFEALKQQSQLI 270

  Fly   264 DTRSEAVTRLIRNQITDYHVLWSQGSKMLSQYREWNEFLNIFGHEAGQKLFRRHMKKLRDDHLNK 328
            ....:....|:...:..::..|...|:.:....|:.:::::.|.:..:|||.:|:.:|:.:|:.:
Frog   271 AAAKDKYEWLVSRVVKSHNETWINQSRRMQSNIEYQDYVHLEGTDKAKKLFHQHVLRLKQEHIER 335

  Fly   329 KLHQYLDKFALALEYLLPDIGALNISDDDAWECARNYLQNHIEFEQYFFECPQASWTELVDMDEA 393
            :...||.....|||.|:||:..:   |..:|....|.|::..||.::|....:..|...:.:|..
Frog   336 RRRNYLLALPRALESLVPDLDEI---DHLSWVKVSNLLESKAEFLKFFIVLEETPWDFTIHIDSI 397

  Fly   394 EDEARIPFDVLETSEAETVFRNYLNSVQQDKKKIGWKQQFKMLLEESGFVTPGKQLSEVRVLFMG 458
            ::  |||:|:|||..||.|:..:|..::..:||...|:.||..|..|.|:||||...|.|...|.
Frog   398 DE--RIPYDLLETVPAEEVYAAHLVKLRNARKKEEMKRSFKENLIASPFITPGKPWEEARSFIMS 460

  Fly   459 RECFEALSEHDCQQIYDIHQDDIIEKSKQNFVELLLEHAQYFLQFKNVDNITQEDVRQITDVIQE 523
            .|.::.|.|....:||..||.:||:|:|:.|.|||||:::.|.:.:.....::|.:..|.||:.|
Frog   461 EEFYQWLEEPVYMEIYSRHQKEIIDKAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGE 525

  Fly   524 DSRYKMLDRLDQERRLMLVQHLRFIHCPIRDHCPFFYNCVDSLIEEVLSDKSA----SNHKTPSG 584
            :.|:|.|.:|..||..::::|:.|::.|.:|.||....|||:.||.:||.:..    ..||.|:
Frog   526 EQRFKALQKLQAERDALILKHIHFVYHPTKDTCPSNPYCVDTRIEHILSSRCTYPYDRLHKDPN- 589

  Fly   585 GGWKSSGSGSDRTLNLLIVGSEHLASDLLNDIR-ICTGSKGEYIYENQTYYLNYRIANGD----M 644
                     .|| :||:|:|.:.||.:|.|:|| :|| :..:|:.:.:.|.|:.|...|:    :
Frog   590 ---------VDR-INLVILGKDGLARELANEIRALCT-NDDKYVIDGKMYELSLRPIEGNVRLPV 643

  Fly   645 EAFKAIDVYSSGLICVYSNQQSFETLKDNLERTLLCNL-ELEDKFENLP--IVLVYQPQDLKENE 706
            .:|:.......|.:|:|::::|...:.:::|::...:| ..|:...:||  ::||.:..|.....
Frog   644 NSFQTSTFLPHGCLCLYNSKESLSYVVESIEKSRESSLGRKENHLIHLPLTLILVNRRGDTSSET 708

  Fly   707 VEYLRNEGMRLSEMLHCDFIDHTQN--------HQKYVYDILNIVILSLKLTEMKSYEPYPSNHT 763
            ...|..:|.:::..|.|.|:|....        ::|.:..||..::.|.:...:.|..|...:.|
Frog   709 AHSLIQQGKQVASKLQCFFLDAASTGMGYGRNINEKQISLILRGLLESKRNLNLVSSTPSIKDLT 773

  Fly   764 D--LRILCCIFCGDQYDIENIVQPLVEESTLVKANEH---------SIIVDVFIGDAKRRVEFIL 817
            |  |||:.|:.|.|.::||:::..|       |.:.:         |:::|:.||..|||:|.:|
Frog   774 DCELRIVMCLMCADIFNIEDVLNAL-------KPHTYRPSQCGLGGSVLLDLPIGSQKRRLELLL 831

  Fly   818 SSYHGTSQYR-DELIHGYIYFYSTKRRSSLANL-SILAAQNANIPLQIIAVTESGGVNAFFNSDI 880
            .|||.:...| ..|:|||:.||:.|||:|||.| :.|......:|:|::|:||  |...|.:. :
Frog   832 LSYHASFNVRKTRLVHGYMIFYAAKRRASLAMLRAFLCEVQDIVPVQLVALTE--GPLTFLDY-V 893

  Fly   881 CQFLITEGNAVADRFKGSFMTFSADQ-YVKFAFYNPFLKTAWDNKYEVENLHV-----------E 933
            .:..:.||..:|...:|.|:.....| ..|...:.|:.|...:.|..:|..|:           |
Frog   894 AREQLQEGEEIAQDIEGKFLILPQGQIQPKLDVFYPYFKDVLEKKVIIEASHMYDNTAEACSTTE 958

  Fly   934 ESITLDSGEGTLENS----VNQMPRPPPRHESYMLSNTLGTDGSGSENYEMAPTRSLNSLNEERD 994
            |..:.......|.||    ..:.|.|.|:|..:.           :|....||.:....|.|...
Frog   959 EVFSSPRAGSPLCNSHLPDSEEDPEPSPQHILFQ-----------NEPNIAAPIKFTRELEETVM 1012

  Fly   995 ISLDEIYDDNEKPKHLHQRFQIFPPPTTPPEPAPPDHQLITCTYKSQFVSASESSLEEITSDVSG 1059
            .:|:...::.            .|||..|..|.|                      .|||
Frog  1013 NTLESKLNNK------------VPPPLKPKPPIP----------------------YEIT----- 1038

  Fly  1060 SKDSLATHDAEWLEDKSDGRRNMNKNLIW---NNFSGSTHAYTTGRRHIDSNLNKIRPKGPSQTL 1121
             |..|:..|.    ...||.|....::.|   ..|..|.:|     ..:||   .::|:...:.:
Frog  1039 -KPELSYLDT----GARDGHRKSLTSINWPPLEAFDPSDYA-----EPMDS---VVKPRNQEENI 1090

  Fly  1122 KVGEAPSRNCPAMSSSTFTLP---TQQPGK-LNMKNFQLVSDAVAKMNFTGSGSGSGSGSGSGST 1182
                             :::|   ||  || :.::|             |.....:|||:||
Frog  1091 -----------------YSVPHDSTQ--GKIITIRN-------------TNKPQSNGSGNGS--- 1120

  Fly  1183 GLGLGLGSGSGCMGDSFLEPVDKDGKRYDHAQLDGEDEDSEELAEYEQIYENEDCTESDSCASST 1247
                                                                    :||...:|.
Frog  1121 --------------------------------------------------------DSDMDTNSL 1129

  Fly  1248 ERRVRQQNAYYKASKKPVAAKKQKKKKVAIPVQTPRVPPFGSYVSPPEIPLHYQRMAVGGS---G 1309
            ||          |.|..:..     |.|....:..::..|.||.|         .::||..   |
Frog  1130 ER----------ARKASIVT-----KPVLYRTKCAKLGKFSSYRS---------SLSVGSDDEPG 1170

  Fly  1310 PGEKKKPEPCVPEFMKSDKSPEYSMVPELAGAGIFGAENLPEYNMNQAKCLKDFEKLEKRRIKEE 1374
            |..:|:.|                       .|..|.:.....|..:|    |.|...||.|...
Frog  1171 PIRRKEEE-----------------------TGTQGTKGDSTTNSYEA----DGEDPRKRNILRS 1208

  Fly  1375 TARQRKLQEKEKEQEKKLKRKLKQNAKGLVESAEAQFGKLMITSEQGEIPIFLNKCVEFIEK--E 1437
            ..|..| :.|.|.:....|...:.|..|...|:       ::|||: .||:|:.||||:||.  |
Frog  1209 LRRTTK-KVKPKPRPSLTKATWESNYFGFPLSS-------VVTSER-PIPVFIEKCVEYIEATGE 1264

  Fly  1438 GLDSEGIYRVPGSRAHVDMLFQRFEEDTNTEIDALDIPVNAVATALKDFFSKRLPPLFSKDIIKE 1502
            |:.:||||||.|:::.::.|.::|::|.|.::...|..||.||.|||.|||:...||...::..|
Frog  1265 GMTTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGALKSFFSELPDPLVPYNMQAE 1329

  Fly  1503 LEEIAGSRGVGNSKLNVEVKTDRSCRLIALKSLLQKLPPINFAILKYIFQHFVHVSDNSKLNSMD 1567
            |.|.        .|:|     |...:|.|:|.||:|.|..|..|.||:..|...||.:..:|.|.
Frog  1330 LVEA--------YKIN-----DLEHKLQAMKDLLKKFPKENHEIFKYVISHLNRVSQHHHINLMT 1381

  Fly  1568 SKNLAICWWPTLIPIDFTDMGHFEQLRPYLEDIVQTMIDQFPYLF 1612
            |:||:||:||||:..|||.|......|.| :.|::..|.|.|:.|
Frog  1382 SENLSICFWPTLMRPDFTTMDALTATRTY-QTIIELFIQQCPFFF 1425

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGAPp190	NP_001259658.1	P-loop_NTPase	<148..238	CDD:304359	38/89 (43%)
		RhoGAP-FF1	254..333	CDD:293121	14/78 (18%)
		RhoGAP_p190	1411..1611	CDD:239838	79/201 (39%)
arhgap35	XP_004917008.1	Ras_like_GTPase	29..245	CDD:206648	114/218 (52%)
		Ras	<158..247	CDD:395024	39/91 (43%)
		RhoGAP-FF1	261..340	CDD:406821	14/78 (18%)
		FF	429..>470	CDD:128718	15/40 (38%)
		FF	485..538	CDD:128718	18/52 (35%)
		pseudoGTPaseD_p190RhoGAP	589..760	CDD:425412	42/182 (23%)
		PHA03151	1036..1225	CDD:177546	60/349 (17%)
		RhoGAP_p190	1234..1420	CDD:239838	79/207 (38%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	194	1.000	Domainoid score	I3117
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H35136
Inparanoid	1	1.050	721	1.000	Inparanoid score	I621
OMA	1	1.010	-	-		QHG50036
OrthoDB	1	1.010	-	-		D110157at2759
OrthoFinder	1	1.000	-	-		FOG0003137
OrthoInspector	1	1.000	-	-		otm48150
Panther	1	1.100	-	-	LDO	PTHR46005
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4737
SonicParanoid	1	1.000	-	-		X2106
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	13	13.110