DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGAPp190 and arhgap35a

DIOPT Version :9

Sequence 1:	NP_001259658.1	Gene:	RhoGAPp190 / 32743	FlyBaseID:	FBgn0026375	Length:	1621	Species:	Drosophila melanogaster
Sequence 2:	XP_001343636.1	Gene:	arhgap35a / 100004299	ZFINID:	ZDB-GENE-120516-2	Length:	1536	Species:	Danio rerio

Alignment Length:	1683	Identity:	479/1683 - (28%)
Similarity:	790/1683 - (46%)	Gaps:	315/1683 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 FNISVIGLSGTEKDRGQVGVGKSCLCNRFMRPMADDYFIDHISVLSQSDFSGRIVNNDHFLYWGD 68
            :|:.|:|||||||::||.|||||||||||:||.|||:.:||.||||.|||.||:|||||||:||:
Zfish    14 YNLVVVGLSGTEKEKGQCGVGKSCLCNRFVRPSADDFHLDHTSVLSTSDFGGRVVNNDHFLFWGE 78

  Fly    69 VRKTTEEGVEYQFNIIEQTEFMDDSTFQAFKVGKMDPYSKRCTATKVFSAEKLMYICKNQLGIEK 133
            ..:..|||.|.:.:::|||||:||.|||..:...:.||.||..:||:.|||||||.|.:|||:|:
Zfish    79 AVRMLEEGAECRMHVVEQTEFIDDQTFQPHRSTALQPYIKRAASTKLASAEKLMYFCTDQLGLEQ 143

  Fly   134 EYEQKVMPDGRLSIDGFVVVFDVSPVPNRSVEKQVEFVQNVIATILKNKKPLVLVTTKNDDAYEL 198
            ::|||.||:|:|.:|||::..|||...||:.:.|::||.|:...:.|.|||:|||.||.|:..|.
Zfish   144 DFEQKQMPEGKLLVDGFLLCVDVSRGMNRNFDDQMKFVTNLYNQLAKTKKPVVLVLTKCDEGVER 208

  Fly   199 YVREAEKISQRKDYKSTVQLIETSAHESINIDLAFLLLAQMIDKVKNRVKIISYQESAKSRKELL 263
            |::::...:..   |..:|::|:||..::|:||||:.|.|:|||.:.:.|||.|.|:.|.:.:.:
Zfish   209 YIKDSHTFALA---KKNLQVVESSARSNVNVDLAFITLVQLIDKSRGKPKIIPYFEALKQQSQQI 270

  Fly   264 DTRSEAVTRLIRNQITDYHVLWSQGSKMLSQYREWNEFLNIFGHEAGQKLFRRHMKKLRDDHLNK 328
            .|..:....|:...:.:::..|...::.:....|:.:::.:.|....:|||::|:.:|:.:|:.:
Zfish   271 ATAKDRYEWLVSRIVKNHNETWPNTNRRMQTSPEYKDYVFLEGTSKAKKLFQQHVHRLKQEHIER 335

  Fly   329 KLHQYLDKFALALEYLLPDIGALNISDDDAWECARNYLQNHIEFEQYFFECPQASWTELVDMDEA 393
            :...||.....||..|:||:..:   |..:|...:..|:...||..:|.......|.....:|..
Zfish   336 RRKIYLSTLPQALNSLVPDLDEI---DHLSWSGVQKVLETKREFSHWFVVLDDTPWETTPHIDNM 397

  Fly   394 EDEARIPFDVLETSEAETVFRNYLNSVQQDKKKIGWKQQFKMLLEESGFVTPGKQLSEVRVLFMG 458
            ||| |||.|:|||..||.::.::|..:..::|:...:.:||..|..|.||||||:..|.|...|.
Zfish   398 EDE-RIPQDLLETPVAEGIYESHLEQLHNERKRAEMRWEFKEKLTCSPFVTPGKRWEEARSFIMN 461

  Fly   459 RECFEALSEHDCQQIYDIHQDDIIEKSKQNFVELLLEHAQYFLQFKNVDNITQEDVRQITDVIQE 523
            .|.::.|.|.:...||:.:|..|.:::|::|.|||||:::.|.:.:.....::|.:..|.:|:.:
Zfish   462 EEFYQWLEEQEYIDIYNKYQKIITDQAKEDFQELLLEYSELFYELEVDAKPSKEKMGAIQEVLGD 526

  Fly   524 DSRYKMLDRLDQERRLMLVQHLRFIHCPIRDHCPFFYNCVDSLIEEVLSDKSASNHKTPSGGGWK 588
            :.|:|.|.:|..||..::::|:.|::.|.::.||...:|||..||:.|:.:..:.:.. .|....
Zfish   527 EQRFKALQKLQAERDALVLKHIHFVYHPTKETCPSSPHCVDGKIEQHLALRYPTRYSF-DGSSKS 590

  Fly   589 SSGSGSDRTLNLLIVGSEHLASDLLNDIR-ICTGSKGEYIYENQTYYLNYRIANGD----MEAFK 648
            ..|.|....:||:|:|.:.||.:|.|:|| :||..: .|:.:.:||.|..|...|:    :.:|.
Zfish   591 QFGEGKVDRINLVILGKDGLARELANEIRALCTNDE-RYVLDGRTYELTLRPIEGNVRLPVNSFH 654

  Fly   649 AIDVYSSGLICVYSNQQSFETLKDNLERTLLCNL-ELEDKFENLPIVL-------VYQPQDLKEN 705
            .......|.:|:|::::|...:.|::|:.....: ..|.....||:.|       |....|:...
Zfish   655 TPTFAPHGCLCLYNSKESLSYVVDSIEKLRESTIGRRETNLVQLPLSLLLVTKRGVGSISDIGGE 719

  Fly   706 EVEYLRNEGMRLSEMLHCDFIDHTQNHQKYVYDI----LNIVILSLKLTEMK-------SYEP-- 757
            ..:.|..:|.:::....|.:::.......|..:|    ||.::..  |.||:       |..|
Zfish   720 TAQTLIQQGQQVAGRFQCSYLEPASPGVGYGRNINDKHLNQILRG--LLEMRRPSVSLGSSSPPL 782

  Fly   758 ----------YPSNHTDLRILCCIFCGDQYDIENIVQPLV------EESTLVKANEHSIIVDVFI 806
                      ..|...||||:.|:.|||.||::.::.|.:      ..|.|  ::..|:::|:.|
Zfish   783 LPSLAGFRDSQQSTEGDLRIVMCLMCGDTYDVDQLLSPFLLPQHCRPLSNL--SSGTSVLLDLSI 845

  Fly   807 GDAKRRVEFILSSYHGT-SQYRDELIHGYIYFYSTKRRSSLANL-SILAAQNANIPLQIIAVTES 869
            |..:..::..:.|||.: |..:..|:||||..||.||::|:..| :.|......||:|::||.|:
Zfish   846 GGQRLSIDLAILSYHSSFSLRKSRLVHGYIAVYSAKRKASMETLCAFLCEVQDIIPVQLLAVAET 910

  Fly   870 GGVNAFFNSDICQFLITEGNAVADRFKGSFMTFSADQ------YVKFAFYNPFLKTAWDNKYEVE 928
            ....|  :|:..:..:.:|..:|......|.:.....      ..:...:.||.|...:.|..||
Zfish   911 QSELA--DSEAVRDQLAQGEELAHEIDARFSSVICGPGGVVGGLHRIDHFQPFFKEVVEKKTIVE 973

  Fly   929 NLHVEESITLDSGEGTLENSVNQMPRPPPRHESYMLSNTLGTDGSGSENYEMAPTRSLNSLNEER 993
            ..|:.:::.        ||..|:....|                       ...:.|:..|:.|.
Zfish   974 ATHMYDNVA--------ENVTNENVSSP-----------------------RCSSPSITLLDSED 1007

  Fly   994 DISLDEIYDDNEKPKHLHQRFQIFPPPTTPPEPAPP------DHQLITCTYK-SQFVSASESSLE 1051
            ||                             ||:||      |..|....:| ......|.|.:.
Zfish  1008 DI-----------------------------EPSPPYPTSRNDDTLTRGGFKLPDLEGDSYSLIS 1043

  Fly  1052 EITSDVSGSKDSLATHDAEWLEDKSDGR----------------RNMNKNLIWNNFSGSTHAYTT 1100
            |||:                ||:|.:.:                :..|.:|           ||.
Zfish  1044 EITN----------------LENKINNKVPPQVRPKPNVTFDIPKTQNSSL-----------YTD 1081

  Fly  1101 GRRHIDSNLNKIRPKGPSQTL-KVGEAPSRNCPAMSSSTFTLPTQQPGKLNMKNFQLVSDAVAKM 1164
            ...| .||...:    ||.|. :.|..||.....|.:.....||::....::.:.......:...
Zfish  1082 LALH-GSNRRSL----PSVTWPEAGYDPSDYAEPMDAVAKPRPTEEENIYSVPHDSTQGKIITIR 1141

  Fly  1165 NFTGSGSGSGSGSGSGSTGLGLGLGSGSGCMGDSFLEPVDKDGKRYDHAQLDGEDEDSEELAEYE 1229
            |...|.| :|:|:||.|
Zfish  1142 NANKSHS-NGAGNGSDS------------------------------------------------ 1157

  Fly  1230 QIYENEDCTESDSCASSTERRVRQQNAYYKASKKPVAAKKQKKKKVAIPVQTPRVPPFGSYVSPP 1294
                     |:||  ||.||| |:.:|   ...||...:.:.|          |:..|.|:.:
Zfish  1158 ---------EADS--SSLERR-RKLSA---LGVKPRLYRDRSK----------RLGKFSSFRT-- 1195

  Fly  1295 EIPLHYQRMAVGGSGPGEKKKPEPCVPEFMKSDKSPEYSMVPELAGAGIFGAENLPEYNMNQAKC 1359
                                       .|:.||  .|....|: |.....||:. .:.::|:
Zfish  1196 ---------------------------SFIGSD--DELGGTPK-AKEDELGAQK-GDTSLNE--- 1226

  Fly  1360 LKDFEKLEKRRIKEETARQRKLQEKEKEQEKKLKRKLKQNAKGLVESAEAQFGKLMITSEQGEIP 1424
              :.|..:||.|.....|:...:.:.|.::..|.:..:.|..| |..|.      ::|.|: .||
Zfish  1227 --EGEDPKKRNILRSLGRRTTKKTRPKARQTSLSKPPESNYFG-VPLAN------VVTPER-PIP 1281

  Fly  1425 IFLNKCVEFIEKEGLDSEGIYRVPGSRAHVDMLFQRFEEDTNTEIDALDIPVNAVATALKDFFSK 1489
            :|:.||:.:||..||.:||||||.|::|.::.:.::|::|.|.::...|:.||.||.|||.|||:
Zfish  1282 LFIEKCIHYIETTGLSTEGIYRVSGNKAEMESMQRQFDQDPNIDLVEKDMSVNTVAGALKSFFSE 1346

  Fly  1490 RLPPLFSKDIIKELEEIAGSRGVGNSKLNVEVKTDRSCRLIALKSLLQKLPPINFAILKYIFQHF 1554
            ...||....:..||.|.        .|:|     ||..||..:|.:|::.|..|:.:.||:..|.
Zfish  1347 LPDPLVPYSMQVELVEA--------FKIN-----DREHRLHTMKDVLRRFPRENYDVFKYVITHL 1398

  Fly  1555 VHVSDNSKLNSMDSKNLAICWWPTLIPIDFTDMGHFEQLRPYLEDIVQTMIDQFPYLF 1612
            ..||.||:||.|.|:||:||:||||:..|||.|......|.| :.|::|.|.|..:.|
Zfish  1399 NKVSLNSRLNLMTSENLSICFWPTLMRPDFTTMDALTATRTY-QTIIETFIHQCAFFF 1455

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGAPp190	NP_001259658.1	P-loop_NTPase	<148..238	CDD:304359	35/89 (39%)
		RhoGAP-FF1	254..333	CDD:293121	13/78 (17%)
		RhoGAP_p190	1411..1611	CDD:239838	77/199 (39%)
arhgap35a	XP_001343636.1	RAB	29..247	CDD:197555	113/220 (51%)
		Ras_like_GTPase	29..245	CDD:206648	112/218 (51%)
		RhoGAP-FF1	261..340	CDD:293121	13/78 (17%)
		FF	431..482	CDD:128718	18/50 (36%)
		RhoGAP_p190	1266..1450	CDD:239838	79/205 (39%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	197	1.000	Domainoid score	I3056
eggNOG	1	0.900	-	-		E1_KOG4271
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H35136
Inparanoid	1	1.050	711	1.000	Inparanoid score	I629
OMA	1	1.010	-	-		QHG50036
OrthoDB	1	1.010	-	-		D110157at2759
OrthoFinder	1	1.000	-	-		FOG0003137
OrthoInspector	1	1.000	-	-		otm24424
orthoMCL	1	0.900	-	-		OOG6_105533
Panther	1	1.100	-	-	LDO	PTHR46005
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4737
SonicParanoid	1	1.000	-	-		X2106
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	14	13.910