Protein Alignment Fim and PLS1

DIOPT Version :9

• Sequence 1: NP_728074.1
• Gene: Fim / 32721
• FlyBaseID: FBgn0024238
• Length: 641
• Species: Drosophila melanogaster
• Sequence 2: NP_001138791.1
• Gene: PLS1 / 5357
• HGNCID: 9090
• Length: 629
• Species: Homo sapiens
• Alignment Length: 621
• Identity: 323/621 - (52%)
• Similarity: 434/621 - (69%)
• Gaps: 26/621 - (4%)

  Fly    14 QEFKQNLEELIPNLDANKDGFIDLHELKDALNQVGFKLAGYQVREMIDEYKGKQLTAFQGKLNLE 78
            :|..:.|:|....:|.:..|::..:||:|...:....|.||:|||::::......:...||::.|
Human    10 REELEELQEAFNKIDIDNSGYVSDYELQDLFKEASLPLPGYKVREIVEKILSVADSNKDGKISFE 74

  Fly    79 EFEALCLDLKSKDVASTFKTVVSKKENLETLGGMSSISSEGTTHSVRLEEQLAFSDWINSNLGHD 143
            ||.:|..:|||||::.||:.:::|:|.:..:||.|:||||||.||...||::||.:|||..|.:|
Human    75 EFVSLMQELKSKDISKTFRKIINKREGITAIGGTSTISSEGTQHSYSEEEKVAFVNWINKALEND 139

  Fly   144 KDLQHLLPIDSEGKRLYLSIKDGILLCKIINHSCPDTIDERAINKKNLTVYREFENLTLALVSSQ 208
            .|.:||:|::.....|:.|:.|||||||:||.|.||||||||||||.||.:...|||.|||.|:.
Human   140 PDCKHLIPMNPNDDSLFKSLADGILLCKMINLSEPDTIDERAINKKKLTPFTISENLNLALNSAS 204

  Fly   209 AIGCNIVNIDAHDLAKGKPHLVLGLLWQIIRIGLFSHITLDSCPGLAGLLFDNERLEDLMKMSPE 273
            ||||.:|||.|.||.:||||||||||||||::|||:.|.:.....|..||.:.|.||:|||:|||
Human   205 AIGCTVVNIGASDLKEGKPHLVLGLLWQIIKVGLFADIEISRNEALIALLNEGEELEELMKLSPE 269

  Fly   274 AILLRWVNHHLERAGISRRCTNFQSDIVDSEIYSHLLKQIA---GNDAD---VNLDALRESDLQS 332
            .:||||||:||..|| ....:||..||.||..|.|||.|||   |.|..   ::|..:.|::...
Human   270 ELLLRWVNYHLTNAG-WHTISNFSQDIKDSRAYFHLLNQIAPKGGEDGPAIAIDLSGINETNDLK 333

  Fly   333 RAEIMLQQAAKLNCRSFLTPQDVVNGVYKLNLAFVANLFNNHPGLDKPEQ-------IEGLESIE 390
            ||.:|||:|.||.|:.|:||.|||:|..||||||||||||.:|.|.||..       :||
Human   334 RAGLMLQEADKLGCKQFVTPADVVSGNPKLNLAFVANLFNTYPCLHKPNNNDIDMNLLEG----- 393

  Fly   391 ETREEKTYRNWMNSMGVAPHVNWLYSDLADGLVIFQLFDVIK-PGIVNWSRVHK-RFSPLRKFME 453
            |::||:|:||||||:||.|::|.|||||||.||||||:::|: |  ||||.|:| .:..|...|:
Human   394 ESKEERTFRNWMNSLGVNPYINHLYSDLADALVIFQLYEMIRVP--VNWSHVNKPPYPALGGNMK 456

  Fly   454 KLENCNYAVDLGK-QLKFSLVGIAGQDLNDGNATLTLALIWQLMRAYTLSILSRLANTGNPIIEK 517
            |:|||||||:||| :.|||||||||||||:||:||||||:|||||.|||::||.|.. |..:.::
Human   457 KIENCNYAVELGKNKAKFSLVGIAGQDLNEGNSTLTLALVWQLMRRYTLNVLSDLGE-GEKVNDE 520

  Fly   518 EIVQWVNNRLSEAGKQSQLRNFNDPAIADGKIVIDLIDAIKEGSINYELVRTSG-TQEDNLANAK 581
            .|::|||..|..|.|::.:.:|.|.:|:....|:||||||...::..|::|... :.||.|.|||
Human   521 IIIKWVNQTLKSANKKTSISSFKDKSISTSLPVLDLIDAIAPNAVRQEMIRRENLSDEDKLNNAK 585

  Fly   582 YAISMARKIGARVYALPEDITEVKPKMVMTVFACMM 617
            ||||:|||||||:||||:|:.|||||||||||||:|
Human   586 YAISVARKIGARIYALPDDLVEVKPKMVMTVFACLM 621

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Fim	NP_728074.1	EF-hand_7	27..84	CDD:290234	16/56 (29%)
		EFh	27..80	CDD:298682	14/52 (27%)
		CH	127..237	CDD:278723	67/109 (61%)
		CH	271..370	CDD:278723	54/104 (52%)
		CH	396..497	CDD:214479	69/103 (67%)
		CH	517..619	CDD:214479	55/102 (54%)
PLS1	NP_001138791.1	SAC6	13..621	CDD:227401	321/616 (52%)
		Actin-binding 1	108..380		152/272 (56%)
		Actin-binding 2	381..625		139/249 (56%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165152964
Domainoid	1	1.000	144	1.000	Domainoid score	I4611
eggNOG	1	0.900	-	-		E2759_KOG0046
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	613	1.000	Inparanoid score	I913
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG54335
OrthoDB	1	1.010	-	-		D138895at33208
OrthoFinder	1	1.000	-	-		FOG0000930
OrthoInspector	1	1.000	-	-		otm40640
orthoMCL	1	0.900	-	-		OOG6_101115
Panther	1	1.100	-	-	LDO	PTHR19961
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X500
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	14	13.770