Protein Alignment Fim and Pls1

DIOPT Version :9

• Sequence 1: NP_728074.1
• Gene: Fim / 32721
• FlyBaseID: FBgn0024238
• Length: 641
• Species: Drosophila melanogaster
• Sequence 2: NP_001028382.1
• Gene: Pls1 / 102502
• MGIID: 104809
• Length: 630
• Species: Mus musculus
• Alignment Length: 622
• Identity: 329/622 - (52%)
• Similarity: 428/622 - (68%)
• Gaps: 27/622 - (4%)

  Fly    14 QEFKQNLEELIPNLDANKDGFIDLHELKDALNQVGFKLAGYQVREMIDEYKGKQLTAFQGKLNLE 78
            :|..:.|:|....:|.:..|::..:||:|...:....|.||:|||::::..........||::.|
Mouse    10 REELEELQEAFNKIDIDNSGYVSDYELQDLFKEASLPLPGYKVREIVEKILVVADNNKDGKISFE 74

  Fly    79 EFEALCLDLKSKDVASTFKTVVSKKENLETLGGMSSISSEGTTHSVRLEEQLAFSDWINSNLGHD 143
            ||.:|..:|||||::.||:.:::|:|.:..:||.||||||||.||...||::||.:|||..|.:|
Mouse    75 EFVSLMQELKSKDISKTFRKIINKREGITAIGGTSSISSEGTQHSYSEEEKVAFVNWINKALEND 139

  Fly   144 KDLQHLLPIDSEGKRLYLSIKDGILLCKIINHSCPDTIDERAINKKNLTVYREFENLTLALVSSQ 208
            .|..||||::.....|:.|:.|||||||:||.|.||||||||||||.||.:...|||.|||.|:.
Mouse   140 ADCSHLLPMNPNDGSLFKSLADGILLCKMINLSEPDTIDERAINKKKLTPFTVSENLNLALNSAS 204

  Fly   209 AIGCNIVNIDAHDLAKGKPHLVLGLLWQIIRIGLFSHITLDSCPGLAGLLFDNERLEDLMKMSPE 273
            ||||.:|||.|.||.:||||||||||||||::|||:.|.:.....|..||.|.|.||:|||:|||
Mouse   205 AIGCTVVNIGAQDLKEGKPHLVLGLLWQIIKVGLFADIEISRNEALIALLKDGEDLEELMKLSPE 269

  Fly   274 AILLRWVNHHLERAGISRRCTNFQSDIVDSEIYSHLLKQIA-------GNDADVNLDALRESDLQ 331
            .:||||||:||..|| .|...||..||.||:.|.|||.|||       |....::|....|.:..
Mouse   270 ELLLRWVNYHLTNAG-WRTINNFSQDIKDSKAYFHLLNQIAPKGDRDDGPAVAIDLSGFNEKNDL 333

  Fly   332 SRAEIMLQQAAKLNCRSFLTPQDVVNGVYKLNLAFVANLFNNHPGLDKPEQ-------IEGLESI 389
            .||..|||:|.||.||.|:||.|||:|..||||||||||||.:|.|.||:.       :||
Mouse   334 KRAGFMLQEADKLGCRQFVTPADVVSGNPKLNLAFVANLFNTYPCLHKPDNNDIDLNLLEG---- 394

  Fly   390 EETREEKTYRNWMNSMGVAPHVNWLYSDLADGLVIFQLFDVIK-PGIVNWSRVHK-RFSPLRKFM 452
             |::||:|:||||||:||.|::|.|||||||.||||||:::|: |  ||||:|:| .:..|...|
Mouse   395 -ESKEERTFRNWMNSLGVNPYINHLYSDLADALVIFQLYEMIRVP--VNWSQVNKPPYPALGGNM 456

  Fly   453 EKLENCNYAVDLGK-QLKFSLVGIAGQDLNDGNATLTLALIWQLMRAYTLSILSRLANTGNPIIE 516
            :|:|||||||:||| :.|||||||||||||:|||||||||:|||||.|||.:||.|.. |..:.:
Mouse   457 KKIENCNYAVELGKNEAKFSLVGIAGQDLNEGNATLTLALVWQLMRRYTLKVLSDLGE-GEKVTD 520

  Fly   517 KEIVQWVNNRLSEAGKQSQLRNFNDPAIADGKIVIDLIDAIKEGSINYELV-RTSGTQEDNLANA 580
            ..|::|||..|..|.|.:.:.:|.|.:|:....|:||||||...::..|:: |...|.||.|.||
Mouse   521 DIIIKWVNQTLKSANKSTSISSFKDKSISTSLPVLDLIDAIAPNAVRQEMIKREHLTDEDKLNNA 585

  Fly   581 KYAISMARKIGARVYALPEDITEVKPKMVMTVFACMM 617
            |||||:|||||||:||||:|:.|||||||||||||:|
Mouse   586 KYAISVARKIGARIYALPDDLVEVKPKMVMTVFACLM 622

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Fim	NP_728074.1	EF-hand_7	27..84	CDD:290234	16/56 (29%)
		EFh	27..80	CDD:298682	14/52 (27%)
		CH	127..237	CDD:278723	68/109 (62%)
		CH	271..370	CDD:278723	55/105 (52%)
		CH	396..497	CDD:214479	70/103 (68%)
		CH	517..619	CDD:214479	56/102 (55%)
Pls1	NP_001028382.1	SAC6	13..622	CDD:227401	327/617 (53%)
		Actin-binding 1	108..381		156/273 (57%)
		Actin-binding 2	382..626		141/249 (57%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167843050
Domainoid	1	1.000	146	1.000	Domainoid score	I4517
eggNOG	1	0.900	-	-		E2759_KOG0046
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	611	1.000	Inparanoid score	I913
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG54335
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000930
OrthoInspector	1	1.000	-	-		otm42714
orthoMCL	1	0.900	-	-		OOG6_101115
Panther	1	1.100	-	-	LDO	PTHR19961
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X500
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	13	12.760