DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CG8915 and Dhx36

DIOPT Version :9

Sequence 1:	NP_001285369.1	Gene:	CG8915 / 32715	FlyBaseID:	FBgn0030833	Length:	976	Species:	Drosophila melanogaster
Sequence 2:	NP_001101148.1	Gene:	Dhx36 / 310461	RGDID:	1308767	Length:	1000	Species:	Rattus norvegicus

Alignment Length:	983	Identity:	295/983 - (30%)
Similarity:	477/983 - (48%)	Gaps:	152/983 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly    37 GGPKGTGKQKTRPGKNAKADAGD------------KLKSED-----EKMLRQLVDDFLQSCEPEK 84
            |||:|:|:.....|..::...|.            .||..:     .|...|      ::.|.|:
  Rat    13 GGPRGSGQGSGGGGGGSRGSGGGGGGRGGRGRHPAHLKGREIGLWYAKKQTQ------KNKEAER 71

  Fly    85 QLPGLTKAQRSHVHRLAQHRG------LKTVSKGPEENRVLFIS--RPQSDG------QQQYILC 135
            |       :|:.|| :.:.|.      |.:|....:::....||  .|:..|      .::.|..
  Rat    72 Q-------ERAVVH-MDERREEQIVQLLNSVQAKNDKDSEAQISWFAPEDHGYGTEVSSEKKINS 128

  Fly   136 NTKLDICPPLLDVLGQMAGNVERRLMNSVGDLKCNKQQYKDIRLPS-----------------NF 183
            ..|||              |.|::|:|         |:.|..|:..                 |.
  Rat   129 EKKLD--------------NQEKKLLN---------QEKKTYRITDKSYIDRDSEYLLQQNEPNL 170

  Fly   184 GLVGQRLIPPPQSN----RNRNIQHERRSLPIYKQRESILNVLQRDQVLIIKGATGSGKSTQLPQ 244
            || .|:|:...|..    |...:|..|:.||.|..::.::|::...||.:|.|.||.||:||:.|
  Rat   171 GL-DQQLLEDLQKKKTDPRYIEMQRFRKKLPSYGMQKELVNLINNHQVTVISGETGCGKTTQVTQ 234

  Fly   245 YILEWAAEH--RAPVRIVVSQPRRIAAISVSERISKERGEA--PGTTVGYNIRMNRQC--SSNTV 303
            :||:...|.  .:..|||.:|||||:||||:||::.||.|:  .|.:.||.||:..:.  ...::
  Rat   235 FILDNYIERGIGSACRIVCTQPRRISAISVAERVAAERAESCGNGNSTGYQIRLQSRLPRKQGSI 299

  Fly   304 LMLTTSGCLLRALAMDKKSFFKNTTHLIIDEAHERDLDTDFLLLATKLELQKNPHLRLVLMSATM 368
            |..|| |.:|:.|..|.:  ..:.:|:::||.|||:|.:|.|:...|..|.....|:::|||||:
  Rat   300 LYCTT-GIILQWLQSDSR--LSSVSHIVLDEIHERNLQSDVLMTVIKDLLHFRSDLKVILMSATL 361

  Fly   369 DLEALSNYFGGGTVMDVEGRSFEVSIYHLEDILSKTGYMHPRMEK-------FLGKPTGKETPSE 426
            :.|..|.|||...::.:.|.:|.|..|.||||:.|..|...:.|.       |:.....::...|
  Rat   362 NAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDIIEKIRYFPEQKEHRSQFKRGFMQGHVNRQEKEE 426

  Fly   427 LLAAY--------------YGGNTI-----VHPD-IDNDLIVSLLELLLRQGDAGAVIVYLPGYS 471
            ..|.|              |..:||     :..| :|.:||.:|:..::.:.:.||::|:|||:.
  Rat   427 KEAIYKERWPAYIKELQTRYSASTIDVLEMMDDDKVDLNLIAALIRYIVLEEEDGAILVFLPGWD 491

  Fly   472 DMTSLLARLESSL--PREQITIILLHSQVDNSEQRKVF-RTYPGVRLKIILSTNIGQTSITIPDL 533
            ::::|...|.|.:  ..::..||.|||.:....|.:|| :|.|||| ||:::|||.:|||||.|:
  Rat   492 NISTLHDLLMSQVMFKSDRFLIIPLHSLMPTVNQTQVFKKTPPGVR-KIVIATNIAETSITIDDV 555

  Fly   534 LYVIDTGLAKMKTYDSTIDASQLTLTWISQADAKQRAGRAGRVCHGNCYRLYDNDRMARMNLYTI 598
            :||||.|..|...:|:..:.|.::..|:|:|:||||.||||||..|:||.||:..|.:.::.|.:
  Rat   556 VYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPGHCYHLYNGLRASLLDDYQL 620

  Fly   599 PEIMRRTLDEICLLTKLAAPDKKIENFLALALDTPPKDAVMQSCSRLKLLGVLDERDEVTPLGHI 663
            |||:|..|:|:||..|:.... .|..||:..:|.|..:||:.|...|..|..||:::|:||||..
  Rat   621 PEILRTPLEELCLQIKILRLG-GIAYFLSRLMDPPSDEAVVLSIKHLMELSALDKQEELTPLGVH 684

  Fly   664 VAELPLGVQIGKCLVYSIYLRCLDSMIIIAAYHSVRDPFVLNIERGKKSGQQISRVLFAGDGMSD 728
            :|.||:...|||.:::.....|||.::.|||..|.:||||:.:  ||:......|...|.:..||
  Rat   685 LARLPVEPHIGKMILFGALFCCLDPVLTIAASLSFKDPFVIPL--GKEKIADARRKELAKETRSD 747

  Fly   729 SLAVIKLYEEFTNLKRKDI---GDFCERNFICRNAMEMFVSAASTLRDTVYRI-FRFSEASARLA 789
            .|.|:..:|.:...||:..   .|:|...|:..|.::|..:......:.:... |..|.:.....
  Rat   748 HLTVVNAFEGWEEAKRRGFRYEKDYCWEYFLSSNTLQMLHNMKGQFAEHLLGAGFVSSRSPKDPK 812

  Fly   790 SSFNNDT-NMIRLALTAGLYPKLA--YMDRENKNQLV---AEGDPLVQVSRSSCLRGKKKQKDLA 848
            ::.|:|. .:|:..:.||||||:|  .::...|.::|   .:.|.||.:...|.   ..:|.|..
  Rat   813 ANINSDNEKIIKAVICAGLYPKVAKIRLNLGKKRKMVKVHTKSDGLVSIHPKSV---NVEQTDFH 874

  Fly   849 SEWILFVEKTRTADQISSLEHTTLVSGLMVALAGGKRFITEAHGSEMLLCLDSWIRLKCPAEFGL 913
            ..|:::..|.||:.  ..|...|.||...:...||...|.:....| ::.:|.||..:.|....
  Rat   875 YNWLIYHLKMRTSS--IYLYDCTEVSPYCLLFFGGDISIQKDKDQE-IIAVDEWIVFQSPERIA- 935

  Fly   914 QLHKVRML 921
              |.|:.|
  Rat   936 --HLVKGL 941

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CG8915	NP_001285369.1	R3H	64..123	CDD:100064	13/71 (18%)
		DEXDc	214..395	CDD:214692	69/186 (37%)
		DEXDc	226..368	CDD:238005	59/147 (40%)
		HELICc	479..575	CDD:197757	44/98 (45%)
		HA2	648..733	CDD:214852	32/84 (38%)
		OB_NTP_bind	783..884	CDD:285018	27/106 (25%)
Dhx36	NP_001101148.1	HrpA	187..893	CDD:224557	240/717 (33%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	257..309		21/52 (40%)
		DEAH box. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00541	326..329		2/2 (100%)
		RecA-like domain 2. /evidence=ECO:0000250\|UniProtKB:Q05B79	379..620		84/241 (35%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	490..549		21/59 (36%)
		Nuclear localization signal. /evidence=ECO:0000250\|UniProtKB:Q9H2U1	509..520		5/10 (50%)
		WH domain. /evidence=ECO:0000250\|UniProtKB:Q05B79	621..690		28/69 (41%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	630..689		22/59 (37%)
		OB-fold-like subdomains. /evidence=ECO:0000250\|UniProtKB:Q05B79	833..897		17/68 (25%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	841..852		2/10 (20%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	862..892		7/34 (21%)
		Necessary for nuclear and nucleolar caps localizations. /evidence=ECO:0000250\|UniProtKB:Q9H2U1	1..192		42/216 (19%)
		Required for the pre-miR-134 transport. /evidence=ECO:0000269\|PubMed:23651854	1..96		20/96 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..53		9/39 (23%)
		Required for recruitment to cytoplasmic stress granules. /evidence=ECO:0000250\|UniProtKB:Q9H2U1	1..43		7/29 (24%)
		Required for G4-DNA-and G4-RNA-binding. /evidence=ECO:0000250\|UniProtKB:Q9H2U1	45..97		13/65 (20%)
		DSM (DHX36-specific motif). /evidence=ECO:0000250\|UniProtKB:Q05B79, ECO:0000250\|UniProtKB:Q9H2U1	45..67		4/27 (15%)
		RecA-like domain 1. /evidence=ECO:0000250\|UniProtKB:Q05B79	98..378		93/306 (30%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG1643
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D222681at33208
OrthoFinder	1	1.000	-	-		FOG0000258
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	5	4.820