DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CG5004 and phldb2b

DIOPT Version :9

Sequence 1:	NP_573195.1	Gene:	CG5004 / 32698	FlyBaseID:	FBgn0260748	Length:	1247	Species:	Drosophila melanogaster
Sequence 2:	XP_021326000.1	Gene:	phldb2b / 560668	ZFINID:	ZDB-GENE-030131-3235	Length:	1471	Species:	Danio rerio

Alignment Length:	1623	Identity:	365/1623 - (22%)
Similarity:	549/1623 - (33%)	Gaps:	572/1623 - (35%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MSLTKKDPSLRVAASDPHLVSLGGGRLSTAVTIHYIHIGDTTIG---SAASCSISLNGSGVRPLH 62
            :.|......|:|..:.|||||||.||||.|:|:..:..|.|.||   :.....|::.|.|:...|
Zfish    35 LDLIDTGKGLKVQTATPHLVSLGSGRLSVAITLLPLKEGVTRIGRDDAPVPQDITIEGPGIEAEH 99

  Fly    63 CTIYRSDANEVTLVPEKDSRLLIDGAPILEETKLSQGAMITIGNSNYLRFNNPDEAQMMRSAMGS 127
            |.| .:....|||.| ......:||.|:...|:|:||..:.:|.|...|||:|:||..|:|.:..
Zfish   100 CRI-ENRGGVVTLDP-CGHLCSLDGVPVTRPTQLTQGYTLCLGKSYIFRFNHPEEANRMKSMLPQ 162

  Fly   128 NERISMPQIDFTQSARQAHELSQSLELESFYESIINPINNPSTYELECPKVFSSDLVTVNMPAKD 192
            ...:|                                           |.|:|:|.:..:.....
Zfish   163 KSPVS-------------------------------------------PLVYSTDYLKFSSDFSH 184

  Fly   193 VL------GQKYASFARNLAENHRNEKQ-LNNQYAKGVGSNGGYWNV---------------PSN 235
            .|      |.:..|..|:|.:..:.:|| |.|.......|:..|:::               .|:
Zfish   185 CLGGSGSRGMRSVSELRDLMDTLQKKKQALENSLRANGDSSPSYFSMTQSPPTTPNLLSPMTSSS 249

  Fly   236 AAIYKEQHQQQHQQAPQDLLTKV------------NNDRYDRYPKAGGLQIYSMNG------VNS 282
            ...|:||.::.:......|.|||            .:|..:|.......:..:.|.      :||
Zfish   250 PNSYQEQARRLYSSERPPLSTKVLPSTTSMPPSPRRSDPRERDTSLPYSRPMTRNQSQDSLLLNS 314

  Fly   283 DINNGPATG-------NNSGTGGGAELEDMLKICTEYADRNNSMPSTHNVSSTSSITSSPIVQNR 340
            ..:...||.       |.|.||....|.                |:....|..:|:.|||.:..|
Zfish   315 SDSRRTATSGSALSMWNGSSTGATDPLP----------------PAPGGGSGAASMPSSPRLARR 363

  Fly   341 IKT-----NGSLPRDKNKSPFPQQAGVASSANISLLSSSSGYENV-RLLGPNRVEI--------- 390
            ..|     ||.|      .|.|:|...::.   ||...||...:: ||..|...::
Zfish   364 FHTPESGRNGGL------EPVPRQRKYSAG---SLTGMSSHSRSLPRLYRPADSQLTLLPIYRSP 419

  Fly   391 NGQI-HVPASAGDGGSSSGVSSNALAPTAAVRASHENLNQNHSATTGGKYVPQSPRTKIRTNCMS 454
            :|.| |:          ||:|.          .:|:|   .||....   :..|.|...:.....
Zfish   420 DGHISHI----------SGISD----------PNHQN---GHSEVIS---ISLSNRPGTKQPVAP 458

  Fly   455 PKKDVSFGNAFALAISPPPLASSAVVSTSK-----------PIPI---------PSMVKPPLAPK 499
            |...::.|.    |.||......::.|||.           |.|.         .|..|..|.
Zfish   459 PDVTMTSGE----ATSPRQAKKVSLTSTSSYSEVDRQAMCGPSPALEIGLGERRQSFGKAGLG-- 517

  Fly   500 PPAPNQQR--------------DYEQLFKSFERKQQQLDRM------------------------ 526
            ||...::|              ||.|..:....::|:::|:
Zfish   518 PPTGFRERKGSISSLSGKEELQDYHQRQRDERLREQEVERLERQRLETILNLCSGLGRAEQDNTG 582

  Fly   527 ---VPAKRSNKNINNLTLN-----------------------LQSVESQTQSPKPSRK------- 558
               ...::.||.:..|.::                       ::..|.|.:..:..|:
Zfish   583 LAVADLQKINKELEKLQVSDDDSTFSDSASLSASISAENDYGIKFREPQAREERQVRQRKNSGHR 647

  Fly   559 -------------PAPAPRSIHLE----------------QRQRRELIQRRKQLKRELAEL--PP 592
                         |.|:||...:.                :.:|.:::...::|:.::.:|  ..
Zfish   648 DARSETIAVRGSAPTPSPRLTRINKTTDDDTQLKNDVRRIEEERIQVLNNMEELELKIKDLDNQM 712

  Fly   593 SAGIEGLELKL----GEQPLAPSASPRLQ-----LQALQNRVCRLEAQRNATRVMEENQQAKLKQ 648
            ...|..||::.    |||   .|...:||     |:.|:.::..:|   |..:..:...:||| .
Zfish   713 EESIRELEVERALLEGEQ---DSEMAQLQQEKDTLEQLKEKMAEIE---NKAQTDKSQDKAKL-D 770

  Fly   649 SIEIKQDQLKKLRAMLKQKPNNA--CLKEELQL-VC---ESLESDRKTFEDLEFQYFE------E 701
            :..:|.::|..|.|.||.:.:..  .|||:||| :|   |:||.:.|.|||||||..|      |
Zfish   771 AERVKLERLANLLAELKTQLDTCPEALKEQLQLQLCRDAETLEVESKRFEDLEFQQLERESRQDE 835

  Fly   702 ESEHHASH-----EEFKR---------------------QEQR-------------LMLEAE--- 724
            |.|....|     .|::|                     |.||             :||:.|
Zfish   836 EKESQTQHILREIAEYQRSTVTRKEKLLALKKQCTQISQQAQRDKDSFVKEKNNLQMMLQREKEN 900

  Fly   725 LAALRIQIGPDEEEPGSPTSPGSTGSNLVNGVMSQSLFGSAELLCPKRRNQEDLMSKSVNENMFY 789
            |.:|..:........|.|.:|.|         |.:..|..|: :||.            :....:
Zfish   901 LVSLEKKYSELTGSLGFPVNPIS---------MKEDCFQLAD-VCPP------------HSEFSH 943

  Fly   790 NHKIEANTSTPKRLPLQLFEDL--------AGGSCEQISFN--LSLQGDRFEVNPLERRVPSQDD 844
            .||    |.:|....|.:|..|        |.|.......|  .|..|.....|||..:..|..|
Zfish   944 AHK----TLSPLPADLLMFSSLLSSLTTKNAEGYVTVTELNELYSQLGVDSSPNPLPTQAQSSPD 1004

  Fly   845 ID-------------RSCKVANDAPISASQGASTKIFDSIMEIERNRKLLLAQQGHQVIEHERQK 896
            ..             .|.....:|.:|.|   |:.:..||          |:........|...|
Zfish  1005 TPAMGTEPSPDPSPAESTAPPGEAELSPS---SSPLSSSI----------LSSSSFTACSHPNPK 1056

  Fly   897 MYDLKKKSH-DEARTQYLLST-------QQSMEQQRQLESDANGGKDAKLFEKTELQKLNQKPGE 953
                ....| .|....:|..:       .:.|.:.||            .|...|.:|...|.|
Zfish  1057 ----PPSVHWPEDMVTFLNPSPPPPPLPAKKMRRHRQ------------HFRSLEERKRYGKEG- 1104

  Fly   954 PVDDQVQKIAGDKENNVQNRKSTGSSPTTT---TTTTTGGGTTPQQQRHSQPELEHHAIALGIGG 1015
                      |...::...||.|  .||.|   |..|.|...:|:    |.|.|..
Zfish  1105 ----------GAHLSDTLPRKKT--QPTITPQFTCATLGRNISPK----SHPPLAQ--------- 1144

  Fly  1016 GGSGGAGGA-NQMVARSPRPL----------------SEANNCDATIEAPKFANGMDSSA----- 1058
              |...|.. |:.:|.||:..                |:.:..|.........:|..||.
Zfish  1145 --SSSCGSVLNRALAISPKETHMDTHRLHKGKSKKGRSQQHVGDEHRTRLSDLSGRASSQTNVYL 1207

  Fly  1059 -SPAVADNKRA-------SSNSQDTASAGSGSGNSGSGGST----ASDRKRVL-----PKHQ--- 1103
             |....||.||       ||:|.||:.:.....|..|..::    ..:.:|:|     .||:
Zfish  1208 DSIGYQDNGRAFDTLSVDSSDSMDTSISACSPDNISSASTSNVLKIEEMERLLREAQAEKHRLLE 1272

  Fly  1104 ----------------------------------------------------RPLTRYLPIFSPD 1116
                                                                ||||||||:...|
Zfish  1273 YREREMESKSRALEEERRRREDLEKRLQEETTRRQKLIEREVKLREKQRAQSRPLTRYLPVRKDD 1337

  Fly  1117 LNLRHHIETAGHQIDPCPHVFVDAHSCRGYLHKLGATFHAWSRRWFVLDRQRSALIYYSDKSERK 1181
            .:||.||::|||..:.|.|:.:...:|||:|.|:|.....|.:||||.||.|..|.||:||.|.|
Zfish  1338 FDLRAHIDSAGHNTETCYHISITEKTCRGFLIKMGGKIKTWKKRWFVFDRNRRTLSYYADKHEAK 1402

  Fly  1182 PRGGAYFATIDEVYLDHL-NASKSGRPHCTFIVKTKKRSYNLQAASDSAARIWIDAIITGAQG 1243
            .:|..||..|:|||.||| :|.||..|..||.|||..|.|.:.|.|..|.|||:|.|:|||:|
Zfish  1403 LKGVIYFQAIEEVYYDHLKSAHKSPNPSLTFSVKTHDRVYYMVAPSPEAMRIWMDVIVTGAEG 1465

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CG5004	NP_573195.1	FHA	41..104	CDD:278899	20/65 (31%)
		PH-like	1142..1242	CDD:302622	52/100 (52%)
		PH	1143..1237	CDD:278594	49/94 (52%)
phldb2b	XP_021326000.1	FHA	51..149	CDD:238017	38/99 (38%)
		Herpes_ICP4_C	189..>500	CDD:332854	73/365 (20%)
		SMC_N	680..>915	CDD:330553	58/241 (24%)
		AccB	<986..>1029	CDD:332248	7/42 (17%)
		PH_PHLDB1_2	1360..1464	CDD:270192	52/103 (50%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	210	1.000	Domainoid score	I2757
eggNOG	1	0.900	-	-		E1_2C17U
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	306	1.000	Inparanoid score	I2617
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D45023at33208
OrthoFinder	1	1.000	-	-		FOG0003925
OrthoInspector	1	1.000	-	-		otm24478
orthoMCL	1	0.900	-	-		OOG6_105283
Panther	1	1.100	-	-	O	PTHR12156
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	11	10.920