Protein Alignment mthl1 and adgre5b.3

DIOPT Version :10

• Sequence 1: NP_573140.1
• Gene: mthl1 / 32637
• FlyBaseID: FBgn0030766
• Length: 676
• Species: Drosophila melanogaster
• Sequence 2: XP_005160275.1
• Gene: adgre5b.3 / 792970
• ZFINID: ZDB-GENE-121214-275
• Length: 756
• Species: Danio rerio
• Alignment Length: 675
• Identity: 135/675 - (20%)
• Similarity: 237/675 - (35%)
• Gaps: 186/675 - (27%)

  Fly    34 MSLAIEEMSPPPAA---PPRPSPPPTVKLNKCCHSGEYLNDGTCIAGSEALWLPMVYLVQQQRFF 95
            :.::.::..|.|.|   |.:.....::.:|.|    :.|||
Zfish   197 IEMSCDQFKPEPDAGNTPEKLKHLMSLLMNSC----KSLND------------------------ 233

  Fly    96 EPHGASPRFLKFLPNTRPTCRK--DQTTEIFRSRGANVMLFPNGTLYVRERALMVQPSDYCVDWE 158
             |||......|.|.|   .|..  |..:|...:.|..:.:|.:.   |.....::.|.
Zfish   234 -PHGEHFTAEKLLEN---LCNSTDDLLSEGNVADGETLSVFLDA---VENSMRLIGPQ------- 284

  Fly   159 VAVVCLNDSQPINALEDPDYAANPLV--QQEPPKASLRLSKCCGKWGS--YNTQLQNCDLQPNHQ 219
                 |.|  |:..:|..:..|...|  .|.||..|:.||.     ||  :||..:..
Zfish   285 -----LKD--PVTKMETQNTYAEVAVTRNQTPPSGSVTLST-----GSAHFNTSWETV------- 330

  Fly   220 AAVDGLLRLSPQLPEGSYQTSYGLPDCGQPGGY--SIAGDWQDA-------KLDRN--TAMLQLP 273
                    :....|..::.......|.......  |:|.:..|.       ||:..  ||::..|
Zfish   331 --------VGKSYPGFAFVALVSYKDMNSSSDLFNSMAREKSDVKEKQITYKLNSKVVTAVVSNP 387

  Fly   274 H-KNLSAG-QYCLEHTQREGEVKIIA-------------------------------CQHLFSSA 305
            . |:||.. .....|.::..|.:.:|                               |.||.|.|
Zfish   388 DTKHLSKPVTLIFTHEEKRAESEGVAYSCVYWSESERGWSGRGCELAWSNSTHTACSCSHLSSFA 452

  Fly   306 AGAGIHDGSIGGTIEQANGQNLQKAVLT--GGILVSIVFLSATLVAGFL--LPAVHHALHWRCQI 366
            ....::      .:|    ...:..|:|  |.:|..|..:...|...|.  :....:::|....:
Zfish   453 VLMALY------PVE----DTFELVVITRVGLVLSLICLILCILTFRFCRSIQGTRNSIHLHLSV 507

  Fly   367 C-YVTCLLFGKILLAIEELSSSLQPGSAACHTLAITMQFFFLAAFFWLNTMCFNIWWTFRDFRPS 430
            | ::..|:|   |..|....:.:     ||..:|..:.||||:||.|:......:      :|..
Zfish   508 CLFIADLVF---LCGISSTHNQV-----ACAIVAGLLHFFFLSAFCWMLLEGVQL------YRMV 558

  Fly   431 SLERNQEALRRYLYSLYAWGGPLLITFVAACVDQLPETTLLRPGFG-QLYCWFDNRNLSIFAYFY 494
            .|..:......|:|:: .:|.||:|..::|..  :|:      |:| ..:||.......|.::|.
Zfish   559 VLVFHTTLKHLYMYAV-GYGVPLVIVSISAIA--VPK------GYGTDRHCWLSLEGYLILSFFI 614

  Fly   495 GPIGLLLCANIALFVSTTHQLTCGLWKRDDVKSSSEK------SALGRVCLKLVVVMGVTWIADI 553
             |:.:::..||.:|:.|..:|.......:...|...|      :|:.::|     |:|.:|:...
Zfish   615 -PVCIIVILNITVFIITVWKLALKFSSLNPDLSKLHKIRSFLATAVAQLC-----VLGGSWVFGF 673

  Fly   554 LSWLVGGPHGVWFFTDLINALQGVFIFIVVGC--QPQVWTACRRIF---CPRLRHDITNTTNGVQ 613
            ..:...|...:.:...::|:|||..||| :.|  ..||....|.:|   |       :|...|..
Zfish   674 FLFQKNGTEVMLYLFIILNSLQGALIFI-MHCLLSKQVRVEYRSLFMRMC-------SNKKKGDY 730

  Fly   614 HSSSSQGLPSMAGGTEITQNTTTTT 638
            ..||::...|.....:|.|:|..:|
Zfish   731 SVSSTEPSHSSQKPLQIDQSTGEST 755

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
mthl1	NP_573140.1	7tmB3_Methuselah-like	326..598	CDD:410632	65/288 (23%)
		TM helix 1	327..352	CDD:410632	6/26 (23%)
		TM helix 2	362..384	CDD:410632	5/22 (23%)
		TM helix 3	396..423	CDD:410632	8/26 (31%)
		TM helix 4	441..461	CDD:410632	6/19 (32%)
		TM helix 5	485..515	CDD:410632	7/29 (24%)
		TM helix 6	530..557	CDD:410632	6/32 (19%)
		TM helix 7	562..587	CDD:410632	8/26 (31%)
adgre5b.3	XP_005160275.1	EGF_CA	32..>62	CDD:214542
		EGF_CA	72..103	CDD:429571
		EGF_CA	<121..152	CDD:214542
		EGF_CA	154..188	CDD:214542
		GPS	416..460	CDD:197639	5/49 (10%)
		7tm_GPCRs	463..723	CDD:475119	66/296 (22%)
		TM helix 1	466..490	CDD:410628	6/23 (26%)
		TM helix 2	499..520	CDD:410628	5/23 (22%)
		TM helix 3	530..552	CDD:410628	8/21 (38%)
		TM helix 4	570..586	CDD:410628	6/16 (38%)
		TM helix 5	605..628	CDD:410628	5/23 (22%)
		TM helix 6	655..677	CDD:410628	5/26 (19%)
		TM helix 7	682..707	CDD:410628	8/25 (32%)