DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment mbt and CLA4

DIOPT Version :9

Sequence 1:	NP_523375.2	Gene:	mbt / 32631	FlyBaseID:	FBgn0025743	Length:	639	Species:	Drosophila melanogaster
Sequence 2:	NP_014101.1	Gene:	CLA4 / 855418	SGDID:	S000005242	Length:	842	Species:	Saccharomyces cerevisiae

Alignment Length:	771	Identity:	217/771 - (28%)
Similarity:	329/771 - (42%)	Gaps:	254/771 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly     7 KKPL---ISMPSNFEHRVHTGFDKRENKYVGLPLQWASIVGNNQILKS--SNRPLPLVDPSEI-- 64
            |.||   :|.|:||.|:||.|||.....:||:|..|..::.:::|...  :|....::...:.
Yeast   177 KCPLLSGVSSPTNFTHKVHVGFDPETGSFVGMPTNWEKLLKHSRITGEDWNNNSAAVIQVLQFYQ 241

  Fly    65 ------TPTEILDLKTIVRPH--------------HNNNKADTTSLNS-SSTMMMGSMAPMNPMA 108
                  .||..||     :|.              :|:||....|:|| ||:.:..||.
Yeast   242 EYNGAGNPTNTLD-----KPQSGETSSSQKSLPNSYNDNKLRNNSVNSKSSSGVSSSMV------ 295

  Fly   109 PGAHPMMSHGPGMMMPPETGGIVLPKTSHVARSNSLRSSSPPRVRRVANVPPSVPEEEGPPAAGT 173
                                              |.|.:|.|            |..:.|.:.|:
Yeast   296 ----------------------------------SQRKTSQP------------PNTKSPVSLGS 314

  Fly   174 PGVGGASSGGFKPPGAHPSLLYNSQHAHANGATGPLAVRTDQTNLQQYRSNLAPPSGGSMPQQQQ 238
                     |..||       .|::            :.|.|:|:.::..|        :|.||.
Yeast   315 ---------GSLPP-------INTK------------LPTSQSNIPRHLQN--------VPNQQY 343

  Fly   239 TSPVGSVASGTRSNHSHTNNGNSGGSYP--PMYPT----SHQQQQQQQQQAKQGGDQ-------- 289
            ..        .|:.||.||     |.:|  ||:|.    |.|||||||||.||...|
Yeast   344 PK--------MRNGHSPTN-----GQFPRGPMHPNNSQRSLQQQQQQQQQQKQQHQQYPYHHQGP 395

  Fly   290 ------NQNPLHPHAHPHPHH--------------------HQHLAKSASRAS------------ 316
                  :.:||:|:   .|||                    :|.:.:.|..:|
Yeast   396 SPSPSPSPSPLNPY---RPHHNMINPYSKQPQSPLSSQSTQNQAIPRYAQNSSPTAAHFQPQRTA 457

  Fly   317 -------------SSSGGASSAAQQ----------------------ASGASGGAAGQPKQDQR- 345
                         |:....|:...|                      |..|..|...:||:..|
Yeast   458 PKPPISAPRAPYPSNQNATSNTHVQPVAPKNDQSTPQTMRQAPKRPDADVAQPGGVAKPKKPARP 522

  Fly   346 -LTHEQFRAALQMVVSAGDPRENLDHFNKIGEGSTGTVCIA-------------------TDKST 390
             ::..:..:.|:.|....||.:......|.|:|::|:|.:|                   .:...
Yeast   523 TMSTAEIMSKLKKVTVNADPSQCFKVIEKAGQGASGSVYLAERTHIPTESNMIELINNDIDEPHV 587

  Fly   391 GRQVAVKKMDLRKQQRRELLFNEVVIMRDYHHPNIVETYSSFL-VNDELWVVMEYLEGGALTDIV 454
            |.:||:|:|.|.||.|:||:.||:::|:|..|.|||....::| .:|:||||||::|||:||||:
Yeast   588 GDKVAIKQMVLSKQPRKELIVNEILVMKDSRHKNIVNFLEAYLRTDDDLWVVMEFMEGGSLTDII 652

  Fly   455 --------THSRMDEEQIATVCKQCLKALAYLHSQGVIHRDIKSDSILLAADGRVKLSDFGFCAQ 511
                    :||.:.|.|||.:.::..:.|.:||.:.:||||||||::||....|||::||||||:
Yeast   653 ENSPTNDNSHSPLTEPQIAYIVRETCQGLKFLHDKHIIHRDIKSDNVLLDTRARVKITDFGFCAR 717

  Fly   512 VSQELPKRKSLVGTPYWMSPEVISRLPYGPEVDIWSLGIMVIEMVDGEPPFFNEPPLQAMRRIRD 576
            ::.:..||.::|||||||:|||:.:..|..::|:||||||.|||::||||:.||.||:|:..|..
Yeast   718 LTDKRSKRATMVGTPYWMAPEVVKQREYDEKIDVWSLGIMTIEMLEGEPPYLNEDPLKALYLIAT 782

  Fly   577 MQPPNLKNAHKVSPRLQSFLDRMLVRDPAQRATAAELLAHPFLRQAGPPSLLVPLM 632
            ...|.||:...:|..::.||...|..|...||:..|||.|.|...|..|..|..|:
Yeast   783 NGTPKLKHPESLSLEIKRFLSVCLCVDVRYRASTEELLHHGFFNMACDPKDLTSLL 838

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mbt	NP_523375.2	CRIB_PAK_like	9..54	CDD:238526	17/49 (35%)
		STKc_PAK_II	360..620	CDD:270815	118/287 (41%)
		S_TKc	372..619	CDD:214567	115/274 (42%)
CLA4	NP_014101.1	PH_Cla4_Ste20	62..174	CDD:270097
		PBD	183..241	CDD:395634	16/57 (28%)
		STKc_PAK	546..826	CDD:270789	116/279 (42%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG0578
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0	Normalized mean entropy	S1363
OMA	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR48015
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	5	4.860