DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment mbt and Pak2

DIOPT Version :9

Sequence 1:	NP_523375.2	Gene:	mbt / 32631	FlyBaseID:	FBgn0025743	Length:	639	Species:	Drosophila melanogaster
Sequence 2:	NP_001380632.1	Gene:	Pak2 / 29432	RGDID:	61953	Length:	524	Species:	Rattus norvegicus

Alignment Length:	637	Identity:	216/637 - (33%)
Similarity:	308/637 - (48%)	Gaps:	186/637 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 SKKKKK--PLISMPSNFEHRVHTGFDKRENKYVGLPLQWASIVGNNQILKSSNRPLPLVDPSEIT 65
            ||||:|  |.||.||:|||.:|.|||....::.|:|.|||.::..:.|.|...:.         .
  Rat    64 SKKKEKERPEISPPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKLEQKK---------N 119

  Fly    66 PTEILDLKTIVRPHHNNNKADTTSLNSSSTMMMGSMAPMNPMAPGAHPMMSHGPGMMMPPETGGI 130
            |..:||    |...:::|......|         |..|
  Rat   120 PQAVLD----VLKFYDSNTVKQKYL---------SFTP--------------------------- 144

  Fly   131 VLPKTSHVARSNSLRSSSPPRVRRVANVPPSVPEEEGPPAAGTPGVGGASSGGFKPPGAHPSLLY 195
                                            ||::|.| :|||.:        ...|:..|.:.
  Rat   145 --------------------------------PEKDGFP-SGTPAL--------NTKGSETSAVV 168

  Fly   196 NSQHAHANGATGP-LAVRTDQTNLQQYRSNLAPPSGGSMPQQQQTSPVGSVASGTRSNHSHTNNG 259
            ..:......|..| :|.|.|.|.....||.:.|     :|     :|||.
  Rat   169 TEEDDDDEDAAPPVIAPRPDHTKSIYTRSVIDP-----IP-----APVGD--------------- 208

  Fly   260 NSGGSYPPMYPTSHQQQQQQQQQAKQGGDQNQNPLHPHAHPHPHHHQHLAKSASRASSSSGGASS 324
                                                                    |:...||.|
  Rat   209 --------------------------------------------------------SNVDSGAKS 217

  Fly   325 AAQQASGASGGAAGQPKQDQRLTHEQFRAALQMVVSAGDPRENLDHFNKIGEGSTGTVCIATDKS 389
            :.:|            |:..::|.|:....|:.:||.|||::....:.|||:|::|||..|||.:
  Rat   218 SDKQ------------KKKAKMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVFTATDVA 270

  Fly   390 TGRQVAVKKMDLRKQQRRELLFNEVVIMRDYHHPNIVETYSSFLVNDELWVVMEYLEGGALTDIV 454
            .|::||:|:::|:||.::||:.||:::|::..:||||....|:||.|||:||||||.||:|||:|
  Rat   271 LGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSYLVGDELFVVMEYLAGGSLTDVV 335

  Fly   455 THSRMDEEQIATVCKQCLKALAYLHSQGVIHRDIKSDSILLAADGRVKLSDFGFCAQVSQELPKR 519
            |.:.|||.|||.||::||:||.:||:..|||||||||::||..:|.|||:|||||||::.|..||
  Rat   336 TETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMEGSVKLTDFGFCAQITPEQSKR 400

  Fly   520 KSLVGTPYWMSPEVISRLPYGPEVDIWSLGIMVIEMVDGEPPFFNEPPLQAMRRIRDMQPPNLKN 584
            .::|||||||:|||::|..|||:|||||||||.||||:||||:.||.||:|:..|.....|.|:|
  Rat   401 STMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQN 465

  Fly   585 AHKVSPRLQSFLDRMLVRDPAQRATAAELLAHPFLRQAGPPSLLVPLMRNAR 636
            ..|:||..:.||:|.|..|..:|.:|.|||.||||:.|.|.|.|.||:..|:
  Rat   466 PEKLSPIFRDFLNRCLEMDVEKRGSAKELLQHPFLKLAKPLSSLTPLILAAK 517

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mbt	NP_523375.2	CRIB_PAK_like	9..54	CDD:238526	19/44 (43%)
		STKc_PAK_II	360..620	CDD:270815	144/259 (56%)
		S_TKc	372..619	CDD:214567	139/246 (57%)
Pak2	NP_001380632.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..81		10/16 (63%)
		Autoregulatory region. /evidence=ECO:0000250	69..137		25/80 (31%)
		GTPase-binding. /evidence=ECO:0000250	69..112		18/42 (43%)
		PBD	73..130	CDD:395634	22/69 (32%)
		Linker	88..248		53/342 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	142..190		15/115 (13%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	204..228		9/106 (8%)
		STKc_PAK2	229..524	CDD:132986	155/289 (54%)
		Nuclear localization signal. /evidence=ECO:0000250	245..251		1/5 (20%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG0578
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D757766at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.820