Protein Alignment Cubn and BMP1

DIOPT Version :10

• Sequence 1: NP_727348.2
• Gene: Cubn / 326235
• FlyBaseID: FBgn0052702
• Length: 3750
• Species: Drosophila melanogaster
• Sequence 2: NP_006120.1
• Gene: BMP1 / 649
• HGNCID: 1067
• Length: 986
• Species: Homo sapiens
• Alignment Length: 966
• Identity: 224/966 - (23%)
• Similarity: 350/966 - (36%)
• Gaps: 292/966 - (30%)

  Fly   762 NTEPGGVSCEYEIHLAVGEQVVIQFARLELDPLDCL-EVLDI---------TDEGGSILQEKICG 816
            |.:||.   ||            .|.::|...::.| |..|.         |...|..|...:..
Human   240 NIQPGQ---EY------------NFLKMEPQEVESLGETYDFDSIMHYARNTFSRGIFLDTIVPK 289

  Fly   817 SDASRLNPPTFTSEFNRLKIKFYARAGSFQLNYRM-ACDYKLNNEQGTITSPGYPNLTRSDRICT 880
            .:.:.:.||  ..:..||.....|:|...   |:. ||...|.:..|..:||.|||...:...|.
Human   290 YEVNGVKPP--IGQRTRLSKGDIAQARKL---YKCPACGETLQDSTGNFSSPEYPNGYSAHMHCV 349

  Fly   881 YTISTATNTVISLKRIDFQLTNGESDDDDNDECLTTNLRINDGLNRK--ILGPYCGKNQPEENFV 943
            :.||......|.|......|.....       |....:.:.||..||  :.|.:||...||. .|
Human   350 WRISVTPGEKIILNFTSLDLYRSRL-------CWYDYVEVRDGFWRKAPLRGRFCGSKLPEP-IV 406

  Fly   944 SETNYLQLHLSTDVDSMGRGFKFEYRALATGNDKCGGVHTRSGDHIRLPVHDDSYAGEATCYWVI 1008
            |..:.|.:...:..:.:|:||...|.|:      |||...:...||:.|.:.|.|.....|.|.|
Human   407 STDSRLWVEFRSSSNWVGKGFFAVYEAI------CGGDVKKDYGHIQSPNYPDDYRPSKVCIWRI 465

  Fly  1009 MAPANKAIRLHWNSFSLENAVDCIYDYLEIYDSLGAQVNDERSKPLAKYCGNSVPEDLLSHSRQL 1073
            .......:.|.:.||.:|....|.|||||:.|.     :.|.|..:.:|||...|:|:.|.|.:|
Human   466 QVSEGFHVGLTFQSFEIERHDSCAYDYLEVRDG-----HSESSTLIGRYCGYEKPDDIKSTSSRL 525

  Fly  1074 VLKFVSDYSESDGGFDLTYTFED----------------------------------------RA 1098
            .||||||.|.:..||.:.: |::                                        .|
Human   526 WLKFVSDGSINKAGFAVNF-FKEVDECSRPNRGGCEQRCLNTLGSYKCSCDPGYELAPDKRRCEA 589

  Fly  1099 KCGGHIHASSGELTSPEYPANYSAGLDCDWHLTGTIDHLLEIQVENFELEQSPNCSADYLEVRNG 1163
            .|||.:...:|.:|||.:|..|....:|.|.|.....:.:.:|.:.||.|.:..|..|::|||:|
Human   590 ACGGFLTKLNGSITSPGWPKEYPPNKNCIWQLVAPTQYRISLQFDFFETEGNDVCKYDFVEVRSG 654

  Fly  1164 GGTDSPLIGRFCGRDIPARIPGFSHEMRLILHTDSAINGRGFRLRWRIFAFGCGGSLRSNMGAIS 1228
            ...||.|.|:|||.:.|..|....:.||:...:|:.::.:||:
Human   655 LTADSKLHGKFCGSEKPEVITSQYNNMRVEFKSDNTVSKKGFK---------------------- 697

  Fly  1229 SPRYPNSYPNMAHCEWRISLHPGSGISLLIEDLELEGLSNCYYDSVKIYTGIKLPNQSPCKVLCK 1293
                       ||                            ::..               |..|.
Human   698 -----------AH----------------------------FFSD---------------KDECS 708

  Fly  1294 DDDLHNPLIQLENNKGTIVFDSDASNTFRGFRISYKANCIRNLTATTGTIESLNYMEPFWETIPI 1358
            .|           |.|.   ..|..|||.    ||:..|
Human   709 KD-----------NGGC---QQDCVNTFG----SYECQC-------------------------- 729

  Fly  1359 NCSWTIRAPKGNRVLVEVSHLARHEQHVPTATMPGGLYIVDGRNVQEIVTPQAMNISGEVLTVVH 1423
                                             ..|..:.|.::
Human   730 ---------------------------------RSGFVLHDNKH--------------------- 740

  Fly  1424 NASNVNFQLDYRIDGCMEELRGTFGFFQSPNYPKMYPNNLECYWLITVEQDSAIELTINNIDLED 1488
                     |.:..||..::..|.|...|||:|..||:..||.|.|:......::||...:|:|.
Human   741 ---------DCKEAGCDHKVTSTSGTITSPNWPDKYPSKKECTWAISSTPGHRVKLTFMEMDIES 796

  Fly  1489 SPNCTKDALTVSNHKNS-VEVHERHCGSTTKLVITSSGHRLHVRFISDNSHNGLGFEATYRTVKA 1552
            .|.|..|.|.|.:.::: ..|..|.|||.....:.::|.|:.:||.||||....||:|::.|
Human   797 QPECAYDHLEVFDGRDAKAPVLGRFCGSKKPEPVLATGSRMFLRFYSDNSVQRKGFQASHAT--- 858

  Fly  1553 TCGGKLTARNGVIESPNYPL----NYPAHSRCEWQVEVSQHH--QIVFEMADLNLESGYDCNWDY 1611
            .|||::.|.....:..::..    |||....|||.:...:.:  ::||:..::..|:  ||.:||
Human   859 ECGGQVRADVKTKDLYSHAQFGDNNYPGGVDCEWVIVAEEGYGVELVFQTFEVEEET--DCGYDY 921

  Fly  1612 LEAYDLTEDDTEGERLFKVCGDETEDDKLLSSSSNMAVVRFISDDSVSKKGFRLHF 1667
            :|.:|  ..|:...||.:.||....::  :.|:.:..:|:|.|||:::||||.|.:
Human   922 MELFD--GYDSTAPRLGRYCGSGPPEE--VYSAGDSVLVKFHSDDTITKKGFHLRY 973

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Cubn	NP_727348.2	cubilin_NTD	21..149	CDD:412063
		EGF_CA	156..190	CDD:238011
		EGF_CA	192..233	CDD:238011
		EGF_CA	282..322	CDD:214542
		EGF_CA	324..367	CDD:214542
		EGF	430..457	CDD:394967
		EGF_CA	469..503	CDD:238011
		CUB	509..622	CDD:238001
		CUB	627..737	CDD:238001
		CUB	744..849	CDD:412131	19/96 (20%)
		CUB	853..970	CDD:238001	31/118 (26%)
		CUB	978..1094	CDD:238001	41/115 (36%)
		CUB	1100..1211	CDD:238001	36/110 (33%)
		CUB	1216..1330	CDD:238001	13/113 (12%)
		CUB	1446..1549	CDD:238001	37/103 (36%)
		CUB	1554..1667	CDD:238001	35/118 (30%)
		CUB	1792..1899	CDD:238001
		CUB	1910..1998	CDD:412131
		CUB	2019..2133	CDD:238001
		CUB	2140..2242	CDD:238001
		CUB	2263..2379	CDD:238001
		CUB	2385..2511	CDD:238001
		CUB	2516..2630	CDD:238001
		CUB	<2833..2892	CDD:412131
		CUB	2898..3008	CDD:238001
		CUB	3011..3127	CDD:238001
		CUB	3130..3241	CDD:238001
		CUB	3254..3363	CDD:238001
		CUB	3379..3508	CDD:238001
		CUB	3531..3601	CDD:412131
		CUB	3623..3733	CDD:238001
BMP1	NP_006120.1	ZnMc_BMP1_TLD	121..320	CDD:239808	20/99 (20%)
		CUB	322..431	CDD:395345	30/116 (26%)
		CUB	435..544	CDD:395345	41/113 (36%)
		FXa_inhibition	555..587	CDD:464251	0/31 (0%)
		CUB	591..700	CDD:395345	38/169 (22%)
		FXa_inhibition	707..742	CDD:464251	13/141 (9%)
		CUB	747..856	CDD:395345	38/108 (35%)
		CUB	860..973	CDD:395345	35/118 (30%)