Protein Alignment Cubn and F8

DIOPT Version :10

• Sequence 1: NP_727348.2
• Gene: Cubn / 326235
• FlyBaseID: FBgn0052702
• Length: 3750
• Species: Drosophila melanogaster
• Sequence 2: NP_000123.1
• Gene: F8 / 2157
• HGNCID: 3546
• Length: 2351
• Species: Homo sapiens
• Alignment Length: 2411
• Identity: 435/2411 - (18%)
• Similarity: 738/2411 - (30%)
• Gaps: 815/2411 - (33%)

  Fly  1502 HKNSVEVHERHCGSTTKL-VITSSGHRLHVRFISDNSHNGLGFEATYRTVKATCGGKLTARNGVI 1565
            |:.||..|....|:|.:: .|...||...||     :|.....|.:..|.       |||:..::
Human   268 HRKSVYWHVIGMGTTPEVHSIFLEGHTFLVR-----NHRQASLEISPITF-------LTAQTLLM 320

  Fly  1566 ESPNYPLNYPAHSRCEWQVEVSQHHQIVFEMADLNLESGYDCNWDYLEAYDLTEDDTEGERLFKV 1630
            :...:.|      .|    .:|.|..                  |.:|||...:...|..:|...
Human   321 DLGQFLL------FC----HISSHQH------------------DGMEAYVKVDSCPEEPQLRMK 357

  Fly  1631 CGDETE--DDKLLSSSSNMAVVRFISDD--------SVSKKGFRLHFHESCGQTIIVDETMFDYI 1685
            ..:|.|  ||.|  :.|.|.||||..|:        ||:||..:...|     .|..:|..:||.
Human   358 NNEEAEDYDDDL--TDSEMDVVRFDDDNSPSFIQIRSVAKKHPKTWVH-----YIAAEEEDWDYA 415

  Fly  1686 QM--------SRQAARNESCLWVFQAVEPNKRIIFTPTHVKLREDANQQ--------YPTEGDCL 1734
            .:        .:....|.....:.:..:..:.:.:|....|.||....:        |...||.|
Human   416 PLVLAPDDRSYKSQYLNNGPQRIGRKYKKVRFMAYTDETFKTREAIQHESGILGPLLYGEVGDTL 480

  Fly  1735 NVGVK-------------------IYEGTEPQGTPRLKFCRSHPPALISNGQALTV------SVP 1774
            .:..|                   :|....|:|...||.....|..:......:||      |.|
Human   481 LIIFKNQASRPYNIYPHGITDVRPLYSRRLPKGVKHLKDFPILPGEIFKYKWTVTVEDGPTKSDP 545

  Fly  1775 LQLVEEFQGHYMTMDTSCGSIYNALSGKFTSPYYPA---------SYPPNIECL--------WLL 1822
             :.:..:...::.|:....|   .|.|.....|..:         |...|:...        |.|
Human   546 -RCLTRYYSSFVNMERDLAS---GLIGPLLICYKESVDQRGNQIMSDKRNVILFSVFDENRSWYL 606

  Fly  1823 ----EASMGNSLSLTLESMDLEKSE------------------------------SCNRDYLE-- 1851
                :..:.|...:.||..:.:.|.                              ....|:|.
Human   607 TENIQRFLPNPAGVQLEDPEFQASNIMHSINGYVFDSLQLSVCLHEVAYWYILSIGAQTDFLSVF 671

  Fly  1852 ----------VREES-----ESGQLIGVYCGNEVPGV---------IHSRG-AIWMKFKSDDDNV 1891
                      |.|::     .||:.  |:...|.||:         ..:|| ...:|..|.|.|.
Human   672 FSGYTFKHKMVYEDTLTLFPFSGET--VFMSMENPGLWILGCHNSDFRNRGMTALLKVSSCDKNT 734

  Fly  1892 GEGFMASY---------------------NYEH----HNELNGT---EGTIESPHFPSKFQDPVP 1928
            |:.:..||                     |..|    ..:.|.|   |..||.       .||.
Human   735 GDYYEDSYEDISAYLLSKNNAIEPRSFSQNSRHPSTRQKQFNATTIPENDIEK-------TDPW- 791

  Fly  1929 YSWRITVDKEYVVAIS--LLYLRDLDQPH-LNF-------YDGYSDIGARIEVTDPDETIISSTN 1983
            ::.|..:.|...|:.|  |:.||....|| |:.       |:.:||        ||....|.|.|
Human   792 FAHRTPMPKIQNVSSSDLLMLLRQSPTPHGLSLSDLQEAKYETFSD--------DPSPGAIDSNN 848

  Fly  1984 V----------------VYFTSNRG-PFKLNWNRLSKEALRSNRTAEERTRQCGNQLI------- 2024
            .                :.||...| ..:|| .:|...|....:..:.:.....|.||
Human   849 SLSEMTHFRPQLHHSGDMVFTPESGLQLRLN-EKLGTTAATELKKLDFKVSSTSNNLISTIPSDN 912

  Fly  2025 ----TIDRSVIGFHSPGYPNGYEQDLNCFWTLVPSNPAMHAVLTLSQIDLEIFSEDCIADYVKIF 2085
                |.:.|.:|  .|..|..|:..|:.......|:|     ||.|...|.:..|:  .|...:.
Human   913 LAAGTDNTSSLG--PPSMPVHYDSQLDTTLFGKKSSP-----LTESGGPLSLSEEN--NDSKLLE 968

  Fly  2086 SG---SDLQNWSELRTLCSLPTESSDRVFHGR----PYLRVE----FVTDPSV---NKTGFNGIV 2136
            ||   |...:|.:     ::.:..|.|:|.|:    |.|..:    |....|:   |||..|.
Human   969 SGLMNSQESSWGK-----NVSSTESGRLFKGKRAHGPALLTKDNALFKVSISLLKTNKTSNNS-- 1026

  Fly  2137 RTACGSEITASKGLV--------NI----TEILKVLP---------------RPNHDCVWTIK-- 2172
            .|...:.|.....|:        ||    ||..||.|               |.||....|..
Human  1027 ATNRKTHIDGPSLLIENSPSVWQNILESDTEFKKVTPLIHDRMLMDKNATALRLNHMSNKTTSSK 1091

  Fly  2173 ----VRQGRRIKI-------DFPDFQL---------------QNNMASG---------------S 2196
                |:|.:...|       |...|::               :|::.||               |
Human  1092 NMEMVQQKKEGPIPPDAQNPDMSFFKMLFLPESARWIQRTHGKNSLNSGQGPSPKQLVSLGPEKS 1156

  Fly  2197 SDCRNYLLLRNGNDEDSPFLGRGKYCEDV-VHEVLNTSSNKAYI----KFHFASPPRFLVSFRFE 2256
            .:.:|:|     ::::...:|:|::.:|| :.|::..||...::    ..|             |
Human  1157 VEGQNFL-----SEKNKVVVGKGEFTKDVGLKEMVFPSSRNLFLTNLDNLH-------------E 1203

  Fly  2257 ELRYTDSGRIRLSASGDEQFISSPYYPHLPHPHS--------ECIWIVEAPPEHRIMLHFQGAFD 2313
            ...:....:|:......|..|.....  ||..|:        :.::::..      ..:.:|::|
Human  1204 NNTHNQEKKIQEEIEKKETLIQENVV--LPQIHTVTGTKNFMKNLFLLST------RQNVEGSYD 1260

  Fly  2314 MLDATGEPEECQREFVLINDGSTELRPEIGRYCGNRKPDTIYSTGNQMR---IRY--FTDVSEPH 2373
                 |......::|..:||.:...:.....:....:.:.:...|||.:   .:|  .|.:| |:
Human  1261 -----GAYAPVLQDFRSLNDSTNRTKKHTAHFSKKGEEENLEGLGNQTKQIVEKYACTTRIS-PN 1319

  Fly  2374 MGFNASLSVARCGGSFHSPEGVIASPSRDLLLIHEEGKQLQECVYTIELEKGSTIDLTSEYLQIP 2438
            .                |.:..:...|:..|      ||.:..:...||||...:|.||....
Human  1320 T----------------SQQNFVTQRSKRAL------KQFRLPLEETELEKRIIVDDTSTQWS-- 1360

  Fly  2439 TLRNGSCSQRNHLMLEEMDAFGLDGEEK--IVDTLMLCGMEAKHLISETNKIVFRYRFLDGIPAE 2501
                   ....||....:.....:.:||  |..:.:...:...|.|.:.|:.......:...|:.
Human  1361 -------KNMKHLTPSTLTQIDYNEKEKGAITQSPLSDCLTRSHSIPQANRSPLPIAKVSSFPSI 1418

  Fly  2502 NQGF--RLKYTSLGSRCGETIYASVGVLQTPGYPLGVPHPMHCKWQVQVPKGRRVRLEILDFN-T 2563
            ...:  |:.:....|......|..        ...||....|.   :|..|...:.|.||... |
Human  1419 RPIYLTRVLFQDNSSHLPAASYRK--------KDSGVQESSHF---LQGAKKNNLSLAILTLEMT 1472

  Fly  2564 GTNMDLRGRLGFRGRLTVANDFKMQSIL---------GRYNVDPPAEVLSSDNTMGIDAFLLPIV 2619
            |...:: |.||.....:|.......::|         |:..:.|...:...|        |.|..
Human  1473 GDQREV-GSLGTSATNSVTYKKVENTVLPKPDLPKTSGKVELLPKVHIYQKD--------LFPTE 1528

  Fly  2620 QNHGIKLRFSAYGSSSCPGF--TVMMNEV---ADIQFQRFNISRPLHCSYKVVPPSNSTLLIRVK 2679
            .::|..........|...|.  .:..||.   ..:.|.|...........|::.|
Human  1529 TSNGSPGHLDLVEGSLLQGTEGAIKWNEANRPGKVPFLRVATESSAKTPSKLLDP---------- 1583

  Fly  2680 EYNTTSVMMWNTHMCALLSPLKFNRLEQEEELMERILCDYQSPAPGKPLPSIRLPFPIQLVVSAS 2744
                   :.|:.|....:...::...|:..|                               ..:
Human  1584 -------LAWDNHYGTQIPKEEWKSQEKSPE-------------------------------KTA 1610

  Fly  2745 ARNAMTNLVLSYSTQSCGGVIILEPGDNMTVHQPSGMVSAAGAIDCAWA---------------- 2793
            .:...|.|.|: :.:|...:..:..|.|    :|.        |:..||
Human  1611 FKKKDTILSLN-ACESNHAIAAINEGQN----KPE--------IEVTWAKQGRTERLCSQNPPVL 1662

  Fly  2794 ------IGPYTDASGEDEVLVPQDIQLEV-----SVYNVNLPAPSPSAQSPEAPCLHHYL----K 2843
                  |...|..|.::|:.....|.:|:     .:|:.:   .:.|.:|.:....|:::    :
Human  1663 KRHQREITRTTLQSDQEEIDYDDTISVEMKKEDFDIYDED---ENQSPRSFQKKTRHYFIAAVER 1724

  Fly  2844 VYNGPDQNSPSLGLFCNQATAVNM-VVERGLFLEYHSDSFSANATFNVSIKYGSGCGGKLVYPY- 2906
            :::....:||.  :..|:|.:.:: ..::.:|.|:...||: ...:...:....|    |:.||
Human  1725 LWDYGMSSSPH--VLRNRAQSGSVPQFKKVVFQEFTDGSFT-QPLYRGELNEHLG----LLGPYI 1782

  Fly  2907 RAIDFAEQYKNNVECIWEVEATMGYHIGLTFQGRF--YIED------------SPGCTKDYL-LV 2956
            ||     :.::|:...:..:|:..|    :|....  |.||            .|..||.|. .|
Human  1783 RA-----EVEDNIMVTFRNQASRPY----SFYSSLISYEEDQRQGAEPRKNFVKPNETKTYFWKV 1838

  Fly  2957 QQRNETTGN------W-----TDLQR--ICGRVAPEMI---NTTSP---------YLRLIFRSDG 2996
            |.....|.:      |     .||::  ..|.:.|.::   ||.:|         ...|.|....
Human  1839 QHHMAPTKDEFDCKAWAYFSDVDLEKDVHSGLIGPLLVCHTNTLNPAHGRQVTVQEFALFFTIFD 1903

  Fly  2997 DVVADGFLAKFERNCGGLLYADSTEQELASPGFPNGYEKYLQCNWTIVPRSPSMGGVLVSFVNFD 3061
            :..:..|....||||..     ....::..|.|...|            |..::.|.::.
Human  1904 ETKSWYFTENMERNCRA-----PCNIQMEDPTFKENY------------RFHAINGYIMD----- 1946

  Fly  3062 LEQGPISVCLYDNLTVTTKDKGKDPQQTTLCGVKHNHEYRGKEYVNLLLRTDGS--------YSG 3118
                                        ||.|:....:.|.:.|    |.:.||        :||
Human  1947 ----------------------------TLPGLVMAQDQRIRWY----LLSMGSNENIHSIHFSG 1979

  Fly  3119 RGFTL---------LYTSRLCGGIISRTSMVESPVQHTDNTLPPGSDCYWNLTAPAGYKFN--IK 3172
            ..||:         ||  .|..|:.....|:            |.....|.:....|...:  :.
Human  1980 HVFTVRKKEEYKMALY--NLYPGVFETVEML------------PSKAGIWRVECLIGEHLHAGMS 2030

  Fly  3173 FLFIDFEANSNCAYDGVEVFSGPIPDERYRWGRFCGRINEDLPLISIPQERGIIHSFSDDRDPSR 3237
            .||:.:   ||.....:.:.||.|.|.:.   ...|:..:..|.::.....|.|:::| .::|..
Human  2031 TLFLVY---SNKCQTPLGMASGHIRDFQI---TASGQYGQWAPKLARLHYSGSINAWS-TKEPFS 2088

  Fly  3238 GFRALVRVMPNCDEKISLNG-----SSRYVYSKFNNAGGYQNDLDCQIVFRVNPDQQISVEFSNF 3297
            ..:..: :.|.....|...|     ||.|: |:|...  |..|......:|.|....:.|.|.|.
Human  2089 WIKVDL-LAPMIIHGIKTQGARQKFSSLYI-SQFIIM--YSLDGKKWQTYRGNSTGTLMVFFGNV 2149

  Fly  3298 HVQDTDGCRSDYVELRDGGGTFADIIGRFCGQNQPPTLRTTRHTLYMRFVTDNKVTDTGFQVTIN 3362
               |:.|.:.:...        ..||.|:...:  ||..:.|.||.|..:        |..:...
Human  2150 ---DSSGIKHNIFN--------PPIIARYIRLH--PTHYSIRSTLRMELM--------GCDLNSC 2193

  Fly  3363 AIP-----RLCGSSEITLSADGTKEVTINSPART------------PGGNYPNGVSCFW------ 3404
            ::|     :....::||.|:..|......||::.            |..|.|..    |
Human  2194 SMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRSNAWRPQVNNPKE----WLQVDFQ 2254

  Fly  3405 ---KIKG------DSLLRVQFVNFDLHGPNQNG----------------SCVDDYLKIYNSEDAP 3444
               |:.|      .|||...:|...|...:|:|                ...|.:..:.||.|.|
Human  2255 KTMKVTGVTTQGVKSLLTSMYVKEFLISSSQDGHQWTLFFQNGKVKVFQGNQDSFTPVVNSLDPP 2319

  Fly  3445 LLEQGL 3450
            ||.:.|
Human  2320 LLTRYL 2325

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Cubn	NP_727348.2	cubilin_NTD	21..149	CDD:412063
		EGF_CA	156..190	CDD:238011
		EGF_CA	192..233	CDD:238011
		EGF_CA	282..322	CDD:214542
		EGF_CA	324..367	CDD:214542
		EGF	430..457	CDD:394967
		EGF_CA	469..503	CDD:238011
		CUB	509..622	CDD:238001
		CUB	627..737	CDD:238001
		CUB	744..849	CDD:412131
		CUB	853..970	CDD:238001
		CUB	978..1094	CDD:238001
		CUB	1100..1211	CDD:238001
		CUB	1216..1330	CDD:238001
		CUB	1446..1549	CDD:238001	13/47 (28%)
		CUB	1554..1667	CDD:238001	29/122 (24%)
		CUB	1792..1899	CDD:238001	29/184 (16%)
		CUB	1910..1998	CDD:412131	28/117 (24%)
		CUB	2019..2133	CDD:238001	33/138 (24%)
		CUB	2140..2242	CDD:238001	31/176 (18%)
		CUB	2263..2379	CDD:238001	19/128 (15%)
		CUB	2385..2511	CDD:238001	22/129 (17%)
		CUB	2516..2630	CDD:238001	22/123 (18%)
		CUB	<2833..2892	CDD:412131	9/63 (14%)
		CUB	2898..3008	CDD:238001	30/150 (20%)
		CUB	3011..3127	CDD:238001	20/132 (15%)
		CUB	3130..3241	CDD:238001	19/112 (17%)
		CUB	3254..3363	CDD:238001	25/113 (22%)
		CUB	3379..3508	CDD:238001	24/115 (21%)
		CUB	3531..3601	CDD:412131
		CUB	3623..3733	CDD:238001
F8	NP_000123.1	CuRO_1_FVIII_like	22..201	CDD:259994
		CuRO_2_FVIII_like	211..345	CDD:259901	25/116 (22%)
		CuRO_3_FVIII_like	399..575	CDD:259889	29/184 (16%)
		CuRO_4_FVIII_like	588..730	CDD:259902	20/143 (14%)
		B	760..1667		188/1083 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	906..928		4/23 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	941..961		6/24 (25%)
		CuRO_5_FVIII_like	1714..1880	CDD:259890	34/181 (19%)
		CuRO_6_FVIII_like	1892..2035	CDD:259904	34/210 (16%)
		FA58C	2042..2187	CDD:238014	36/173 (21%)
		FA58C	2195..2344	CDD:238014	28/135 (21%)