DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Sk2 and Sphk2

DIOPT Version :9

Sequence 1:	NP_647762.3	Gene:	Sk2 / 326219	FlyBaseID:	FBgn0052484	Length:	661	Species:	Drosophila melanogaster
Sequence 2:	NP_001166032.1	Gene:	Sphk2 / 56632	MGIID:	1861380	Length:	617	Species:	Mus musculus

Alignment Length:	632	Identity:	194/632 - (30%)
Similarity:	282/632 - (44%)	Gaps:	126/632 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly    85 MRPKKSRR-----LAMSGACACSSGNPNSPAISASGDHHRPATTPSKCSTNSRDNIPSDGGDVSA 144
            :|||...|     :::.....|.:....||.                              |.:|
Mouse    44 LRPKPEARPRDGLVSLDEVSGCGTLQSRSPE------------------------------DTAA 78

  Fly   145 FLYVFAYVLKKRSLRSELHRERTVLTLRFRSFDTFEDNMREADRWYRSLRWQLHRTL----EEIF 205
            :..::.|...:|.     .|.|...|.|.....|:|:|..||.||..:|...|....    :||
Mouse    79 YFCIYTYPRGRRG-----GRRRATRTFRADGATTYEENRAEAQRWATALTCLLRGVPLSGDQEI- 137

  Fly   206 VAPTVDERRRRVLVLLNPKSGSGDAREVFNMHVTPVLNEAEVPYDLYVTKHSNFAIEFLSTRCLD 270
             .|.:..|:.|:|:|:||..|.|.|.:....||.|:::||.:.::|..|:..|.|.|.:....|.
Mouse   138 -TPELLPRKPRLLILVNPFGGRGLAWQRCMDHVVPMISEAGLSFNLIQTERQNHARELVQGLSLS 201

  Fly   271 AWCCVVAVGGDGLFHEIVNGLLQRQDWAHVLPHLALGIIPCGSGNGLARSIAHCYNEPYFSKPV- 334
            .|..:|.|.||||.:|::||||.|.||...: .:.:|::||||||.||.::.|   ...|.:.|
Mouse   202 EWEGIVTVSGDGLLYEVLNGLLDRPDWEDAV-RMPIGVLPCGSGNALAGAVNH---HGGFEQVVG 262

  Fly   335 ----LGAALTVISGRSSPMDVVRVQLQSRS-LYSFLSIGWGLISDVDIESERIRMLGYQRFTVWT 394
                |..:|.:..|.|.|:|::.|.|.|.| .:||||:.||.:|||||.|||.|.||..|||:..
Mouse   263 VDLLLNCSLLLCRGGSHPLDLLSVTLASGSRCFSFLSVAWGFLSDVDIHSERFRALGSARFTLGA 327

  Fly   395 LYRLVNLRTYNGRISYL---------LTDHEV---------------SSTHSATGYAAQRRMQSS 435
            :..|.:|.||.||:|||         :..|.:               ::|||..          .
Mouse   328 VLGLASLHTYRGRLSYLPATTEPALPIPGHSLPRAKSELVLAPAPAPAATHSPL----------H 382

  Fly   436 RSCNTHIDMLNGPAPIYHSSAEYLPQEFADVISLETSINQSFRSRCDSWLSGGSRRSFYYSISES 500
            ||.:.....|..||.:...|.|.||         :.|:|.........|...|........:..|
Mouse   383 RSVSDLPLPLPQPALVSPGSPEPLP---------DLSLNGGGPELTGDWGGAGDAPLSPDPLLPS 438

  Fly   501 IYHSLADESEFAGLAAASLE--------------NRQQNYGPASE-LPDLNEPLSEDQGWLVEEG 550
            ..::| ..::.:.:|....|              .....:||... ||.|..||.:|  |:..||
Mouse   439 SPNAL-KTAQLSPIAEGPPEMPASSGFLPPTHSAPEASTWGPVDHLLPPLGSPLPQD--WVTIEG 500

  Fly   551 EFVMMHAVYQTHLGIDCHFAPKAQLNDGTIYLILIRAGISRPHLLSFLYNMSSGTHL----PESH 611
            |||:|..:..:||..|...||.|:.:||.::|..:|:||||..||..|..|..|.|.    |
Mouse   501 EFVLMLGILPSHLCADLMAAPHARFDDGVVHLCWVRSGISRAALLRILLAMEHGNHFSLGCP--- 562

  Fly   612 DDHVKVLPVRAFRLEPYDNHGIITVDGERVEFGPLQAEVLPGIARVM 658
              |:.....|||||||....|::|||||.||:||:||:|.||:|.::
Mouse   563 --HLGYAAARAFRLEPLTPRGLLTVDGELVEYGPIQAQVHPGLATLL 607

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Sk2	NP_647762.3	PLN02958	<213..639	CDD:215517	155/474 (33%)
Sk2	NP_647762.3	DAGK_cat	216..354	CDD:279163	52/142 (37%)
Sphk2	NP_001166032.1	Required for binding to sulfatide and phosphoinositides and for membrane localization. /evidence=ECO:0000250	1..140		26/132 (20%)
		NLS. /evidence=ECO:0000269\|PubMed:12954646	87..95		2/12 (17%)
		PLN02958	<142..608	CDD:215517	167/497 (34%)
		Substrate binding. /evidence=ECO:0000250\|UniProtKB:Q9NYA1	210..213		1/2 (50%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	371..472		20/120 (17%)
		NES. /evidence=ECO:0000250\|UniProtKB:Q9NRA0	381..390		2/18 (11%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167836245
Domainoid	1	1.000	150	1.000	Domainoid score	I4375
eggNOG	1	0.900	-	-		E1_COG1597
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	260	1.000	Inparanoid score	I3101
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG47523
OrthoDB	1	1.010	-	-		D681139at2759
OrthoFinder	1	1.000	-	-		FOG0001465
OrthoInspector	1	1.000	-	-		otm43305
orthoMCL	1	0.900	-	-		OOG6_101566
Panther	1	1.100	-	-	O	PTHR12358
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R653
SonicParanoid	1	1.000	-	-		X918
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	14	13.840