Protein Alignment para and SCN4A

DIOPT Version :10

• Sequence 1: NP_001259619.1
• Gene: para / 32619
• FlyBaseID: FBgn0285944
• Length: 2145
• Species: Drosophila melanogaster
• Sequence 2: NP_000325.4
• Gene: SCN4A / 6329
• HGNCID: 10591
• Length: 1836
• Species: Homo sapiens
• Alignment Length: 2136
• Identity: 921/2136 - (43%)
• Similarity: 1229/2136 - (57%)
• Gaps: 429/2136 - (20%)

  Fly    17 RPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQ 81
            ||||||||..||||..   |::..|:|             .||.||    |:.:..|:.|  ||.
Human    18 RPFTRESLAAIEQRAV---EEEARLQR-------------NKQMEI----EEPERKPRSD--LEA 60

  Fly    82 GVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVA 146
            |..:|: :.|..|||:...||||:||||||..||:|::|||.||||||:.|:::|.||:.:||.|
Human    61 GKNLPM-IYGDPPPEVIGIPLEDLDPYYSNKKTFIVLNKGKAIFRFSATPALYLLSPFSVVRRGA 124

  Fly   147 IYILVHPLFSLFIITTILVNCILMIMPTTPT-VESTEVIFTGIYTFESAVKVMARGFILCPFTYL 210
            |.:|:|.|||:||:.|||.||:.|.|...|. .::.|..|||||||||.:|::||||.:..||:|
Human   125 IKVLIHALFSMFIMITILTNCVFMTMSDPPPWSKNVEYTFTGIYTFESLIKILARGFCVDDFTFL 189

  Fly   211 RDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVI 275
            ||.||||||.||.:||:|..:||||::|||||||||||||:.::|||||||||:|:|||.|.||:
Human   190 RDPWNWLDFSVIMMAYLTEFVDLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVM 254

  Fly   276 ILTMFSLSVFALMGLQIYMGVLTQKCIKKFP----------LDGSW-GNLT-------------- 315
            |||:|.||||||:|||::||.|.|||::..|          .:.:| ||.|
Human   255 ILTVFCLSVFALVGLQLFMGNLRQKCVRWPPPFNDTNTTWYSNDTWYGNDTWYGNEMWYGNDSWY 319

  Fly   316 -DENWDYH----NRNSSNW--YSEDEGISF--------PLCGNISGAGQCDDDYVCLQGFGPNPN 365
             ::.|:.|    ..::.:|  |..|||..:        .||||.|.||.|.:.|.|:: .|.|||
Human   320 ANDTWNSHASWATNDTFDWDAYISDEGNFYFLEGSNDALLCGNSSDAGHCPEGYECIK-TGRNPN 383

  Fly   366 YGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAM 430
            |||||:|:|.||||:.|||||||:||:|:||.|||||..:|:||:||||||||||:|||||:|||
Human   384 YGYTSYDTFSWAFLALFRLMTQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVAM 448

  Fly   431 SYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPT 495
            :|.|..:....|:..:||..::..|.......:||:...|||
Human   449 AYAEQNEATLAEDKEKEEEFQQMLEKFKKHQEELEKAKAAQA----------------------- 490

  Fly   496 YSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLP 560
                    ..|||...|                         ||
Human   491 --------LEGGEADGD-------------------------PA--------------------- 501

  Fly   561 GSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGA 625
                                                                             
Human   502 ----------------------------------------------------------------- 501

  Fly   626 IIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNG 690
                                        ||.
Human   502 ----------------------------HGK---------------------------------- 504

  Fly   691 GTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRH 755
                 |.|..||          |.:.                                |:.|.|.
Human   505 -----DCNGSLD----------TSQG--------------------------------EKGAPRQ 522

  Fly   756 SRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFV 820
            |.:.|.|:|......|:..:..|.:..|....:|       :|:||..||||:..:.|||.||||
Human   523 SSSGDSGISDAMEELEEAHQKCPPWWYKCAHKVL-------IWNCCAPWLKFKNIIHLIVMDPFV 580

  Fly   821 ELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIF 885
            :|.||:|||:||:||||:|:.|.:..:.||..||..||..|..|..:||:||.|..|||:|||||
Human   581 DLGITICIVLNTLFMAMEHYPMTEHFDNVLTVGNLVFTGIFTAEMVLKLIAMDPYEYFQQGWNIF 645

  Fly   886 DFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCII 950
            |.|||.|||:||||..||||||||||||||||||||||||||:||.|:|.::|||||||.||.||
Human   646 DSIIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAII 710

  Fly   951 IFIFAVMGMQLFGKNYHDHKDRFP-DGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYV-GD 1013
            :|||||:|||||||:|.:...:.. |.:||||:..||.|||:||||:|||||||:|||||.| |.
Human   711 VFIFAVVGMQLFGKSYKECVCKIALDCNLPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQ 775

  Fly  1014 VSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNI 1078
            ..|:..||..:|||||||||||||||||:|.:.||:|...|.:.|.:..|   |||.|..:....
Human   776 AMCLTVFLMVMVIGNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIA---IGRIKLGIGFAK 837

  Fly  1079 ADCFKLIRNKLTNQISDQPS-GERTNQISWIWSEGKGVCRCISAEHG-----DNELELGHDEILA 1137
            |....|:..|:.:......| ||         ::|.|.    :.|.|     |.:.|...:::..
Human   838 AFLLGLLHGKILSPKDIMLSLGE---------ADGAGE----AGEAGETAPEDEKKEPPEEDLKK 889

  Fly  1138 DGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKD 1202
            |..|..        .:.:.||...::..|..|       ::||........|.|..|....|.::
Human   890 DNHILN--------HMGLADGPPSSLELDHLN-------FINNPYLTIQVPIASEESDLEMPTEE 939

  Fly  1203 ESHKGSAETMEGEEKRDASKEDLGLDE--------------ELDEEGECEEGPLDGDIIIHAHDE 1253
            |:...|       |..|:.|....|.:              |.|.|.:.||.| :|
Human   940 ETDTFS-------EPEDSKKPPQPLYDGNSSVCSTADYKPPEEDPEEQAEENP-EG--------- 987

  Fly  1254 DILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLAL 1318
                |.|.:|..::..:::|.|..|......:.|..||...|:::|:.:|||.::.|||:||.||
Human   988 ----EQPEECFTEACVQRWPCLYVDISQGRGKKWWTLRRACFKIVEHNWFETFIVFMILLSSGAL 1048

  Fly  1319 ALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFV 1383
            |.||:::.||.:::.||.|.|::||.||.:|||:||:|.|||||||||||||||:||.||:|:.|
Human  1049 AFEDIYIEQRRVIRTILEYADKVFTYIFIMEMLLKWVAYGFKVYFTNAWCWLDFLIVDVSIISLV 1113

  Fly  1384 ASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMG 1448
            |:.:|...:...|::|||||||||||:||.:|||||||||:.|||||.|||||||||||||:|||
Human  1114 ANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMG 1178

  Fly  1449 VQLFAGKYFKCEDMNGT---KLSHEIIPNRNACESENYT----WVNSAMNFDHVGNAYLCLFQVA 1506
            |.|||||::.|  :|.|   :.....:.|::.|||..:|    |:|..:|:|:||..||.|.|||
Human  1179 VNLFAGKFYYC--INTTTSERFDISEVNNKSECESLMHTGQVRWLNVKVNYDNVGLGYLSLLQVA 1241

  Fly  1507 TFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAG 1571
            |||||:.||..|:||||.::||..|.|:|||||||.||||||||||||||||||||||:||||.|
Human  1242 TFKGWMDIMYAAVDSREKEEQPQYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLG 1306

  Fly  1572 GSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTM 1636
            |. ::||||:||||||||||:|||||.|.||||:.:.|.:|:::||.:.|||.||:.|.|||.||
Human  1307 GK-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPQNKIQGMVYDLVTKQAFDITIMILICLNMVTM 1370

  Fly  1637 TLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSD 1701
            .::..:.|.....:|..:|.||::||:.||:||:.|||.:||...||:||.|||||||:||.|||
Human  1371 MVETDNQSQLKVDILYNINMIFIIIFTGECVLKMLALRQYYFTVGWNIFDFVVVILSIVGLALSD 1435

  Fly  1702 IIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFG 1766
            :|:||||||||.||:|:|::||||||::||||||||||||.|||||||||.|||||||||::|||
Human  1436 LIQKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFG 1500

  Fly  1767 MSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINE--EACDPPDNDKG--YP 1827
            ||.|.:||::|||:|::||:|||.|:|.||:::|||||||:|:.|:|.  ..|||...:.|  ..
Human  1501 MSNFAYVKKESGIDDMFNFETFGNSIICLFEITTSAGWDGLLNPILNSGPPDCDPNLENPGTSVK 1565

  Fly  1828 GNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPE 1892
            |:||:.::||.|..||::||||||:|||||:||||::.|||:..|.|.:||::|:||.|::|||:
Human  1566 GDCGNPSIGICFFCSYIIISFLIVVNMYIAIILENFNVATEESSEPLGEDDFEMFYETWEKFDPD 1630

  Fly  1893 GTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKG--N 1955
            .||:|.|.:||:|:|.|:.||:|.||||.|:|::|:|:..||.::|:|||.||||:.....|  :
Human  1631 ATQFIAYSRLSDFVDTLQEPLRIAKPNKIKLITLDLPMVPGDKIHCLDILFALTKEVLGDSGEMD 1695

  Fly  1956 PIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKH-KARGEGGGSFEPDTDHGD 2019
            .:::|.|...:||.|....|||:::||.|:.||.||..||.|:|:| ..|.....|:.....| |
Human  1696 ALKQTMEEKFMAANPSKVSYEPITTTLKRKHEEVCAIKIQRAYRRHLLQRSMKQASYMYRHSH-D 1759

  Fly  2020 GGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAA 2075
            |    :||.||::          :|.:..|.......:..|.:||..:....|..|
Human  1760 G----SGDDAPEK----------EGLLANTMSKMYGHENGNSSSPSPEEKGEAGDA 1801

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
para	NP_001259619.1	Ion_trans	152..436	CDD:459842	174/324 (54%)
		Na_trans_cytopl	555..740	CDD:463401	7/184 (4%)
		Ion_trans	817..1028	CDD:459842	137/212 (65%)
		Na_trans_assoc	1054..1296	CDD:461936	53/261 (20%)
		Ion_trans	1300..1571	CDD:459842	171/277 (62%)
		Na_channel_gate	1561..1613	CDD:240441	35/51 (69%)
		Ion_trans	1621..1871	CDD:459842	153/253 (60%)
		GPHH	1882..1929	CDD:465306	25/46 (54%)
SCN4A	NP_000325.4	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	39..63		12/42 (29%)
		I. /evidence=ECO:0000305	113..454		182/341 (53%)
		Ion_trans	130..457	CDD:459842	174/327 (53%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	493..530		18/256 (7%)
		II. /evidence=ECO:0000305	560..832		172/274 (63%)
		Ion_trans	577..790	CDD:459842	137/212 (65%)
		Na_trans_assoc	816..1026	CDD:461936	53/261 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	863..885		6/25 (24%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	930..992		17/82 (21%)
		III. /evidence=ECO:0000305	1013..1326		191/315 (61%)
		Ion_trans	1030..1304	CDD:459842	169/275 (61%)
		Na_channel_gate	1296..1348	CDD:240441	36/52 (69%)
		Important for rapid channel inactivation. /evidence=ECO:0000250|UniProtKB:P15390	1310..1312		0/1 (0%)
		IV. /evidence=ECO:0000305	1335..1633		172/297 (58%)
		Ion_trans	1353..1609	CDD:459842	153/255 (60%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1778..1836		5/24 (21%)