Protein Alignment para and scn4aa

DIOPT Version :10

• Sequence 1: NP_001259619.1
• Gene: para / 32619
• FlyBaseID: FBgn0285944
• Length: 2145
• Species: Drosophila melanogaster
• Sequence 2: NP_001034914.2
• Gene: scn4aa / 572442
• ZFINID: ZDB-GENE-051201-2
• Length: 1829
• Species: Danio rerio
• Alignment Length: 2042
• Identity: 902/2042 - (44%)
• Similarity: 1194/2042 - (58%)
• Gaps: 399/2042 - (19%)

  Fly    14 SLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPT 78
            |:||.||.||||:||:.|      |::..|:..||.                 |:|.:.|..|
Zfish    11 SVFRRFTPESLVEIERLI------QEKSTREELEGA-----------------EEEPQAPSSD-- 50

  Fly    79 LEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIR 143
            ||.|..:|: :.|..|.:|.:||||||||:|....||:|:|||..|||||:..||:.:.||:.:|
Zfish    51 LEAGKCLPM-IYGDPPGDLLNTPLEDIDPFYKTQKTFIVISKGNTIFRFSSEPAMFCISPFSIVR 114

  Fly   144 RVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVEST-EVIFTGIYTFESAVKVMARGFILCPF 207
            |.||.||:|.|||:||:.|||.||:.|.|...|....| |.:||||||||:.|||::|||.:.||
Zfish   115 RGAIKILIHSLFSMFIMITILSNCVFMTMSNPPAWSKTVEYVFTGIYTFEATVKVLSRGFCIGPF 179

  Fly   208 TYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLR 272
            |:|||.||||||:||::||||..:||||::|||||||||||||:.::|||||||||:|:|||.:.
Zfish   180 TFLRDPWNWLDFMVISMAYVTEFVDLGNVSALRTFRVLRALKTITVIPGLKTIVGALIQSVKKMI 244

  Fly   273 DVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSN--------- 328
            ||:|||:|:|:||||:|||::||.|.||||:       |..|....:|.:|.|..|
Zfish   245 DVMILTIFALAVFALIGLQLFMGNLRQKCIR-------WPILNSTIFDVYNSNMVNDTTLNVTDT 302

  Fly   329 -----WYSEDEGISF-------PLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSA 381
                 :.|.:|...|       .||||.|.||:|.:.|.|::. |.||||||||:|:||||||:.
Zfish   303 FDFKAYISNEENQYFLEGSLDALLCGNSSDAGRCPEGYTCMKA-GRNPNYGYTSYDNFGWAFLAL 366

  Fly   382 FRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAE 446
            ||||||||||:|:||.|||||..:|:||:|:||||||||:|||||:|||:||| |.:|...||.:
Zfish   367 FRLMTQDFWENLFQLTLRAAGKTYMIFFVVVIFLGSFYLINLILAVVAMAYDE-QNEATLAEARD 430

  Fly   447 EEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGN 511
            :|     ||....    ||:..|.:..:.|..|:
Zfish   431 KE-----EEFQRL----LEQLKNQETGSKASLAS------------------------------- 455

  Fly   512 DDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKY 576
                                             |.|:.|..:.|     ||..::      :.:.
Zfish   456 ---------------------------------QKTQSRGSNRT-----GSLHDL------ADED 476

  Fly   577 TIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSS 641
            .|::..||.       .|.::.....:.:.|...:|..                   :|.|:||.
Zfish   477 VIKDCNGRI-------VPRLIVNRVSSNKELSAEEDQK-------------------SLSSKHSM 515

  Fly   642 YTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDY 706
            ....|.::|..:       .:.:.|.|.|.|
Zfish   516 QYLDQPKLSKRT-------ASALSVLTATME---------------------------------- 539

  Fly   707 EIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTE 771
              |||..                                          .|....|   :|
Zfish   540 --GLEDA------------------------------------------QRPCPPG---WY---- 553

  Fly   772 DDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMA 836
                              |..|:|..||||..|:.|::||..:|.||||:|.||:|||:||:|||
Zfish   554 ------------------KFADMFLKWDCCAPWILFKKWVHFVVMDPFVDLGITICIVLNTLFMA 600

  Fly   837 MDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEG 901
            |:|:.|:...|.||..||..||..|..|...||:||.|.||||.||||||.|||.|||:||||..
Zfish   601 MEHYPMSPHFEHVLSVGNLVFTGIFTAEMVFKLIAMDPYYYFQVGWNIFDSIIVTLSLVELGLAN 665

  Fly   902 VQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNY 966
            ||||||||||||||||||||||||||:||.|:|.::|||||||.||.||:|||||:|||||||:|
Zfish   666 VQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSY 730

  Fly   967 HDHKDRF-PDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVS-CIPFFLATVVIGNL 1029
            .|...:. .|.:||||:..||.|||:||||:|||||||:|||||.|...| |:..|:..:|||||
Zfish   731 KDCVCKISEDCELPRWHMNDFFHSFLIVFRILCGEWIETMWDCMEVAGASMCLIVFMMVMVIGNL 795

  Fly  1030 VVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQIS 1094
            ||||||||||||:|...:||....|.:.|.:..|..||.|...|||..:|...:.|..|..:...
Zfish   796 VVLNLFLALLLSSFSGDNLSGGDDDGEMNNLQIAIGRITRGIDWVKALVASMVQRILGKKPDNTK 860

  Fly  1095 DQPSGE----RTNQISWIWSEGK---GVCRCIS----AEHGDNELELGHDEILADGLIKKGIKEQ 1148
            ::..|:    ..|.:    .|||   |:..|:|    ......|.::..||
Zfish   861 EEGEGDIELYALNHL----DEGKMADGLTNCLSPTLTVPIARCESDVEEDE-------------- 907

  Fly  1149 TQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETME 1213
                             |.:::..:.:|...|..|....|...|...:..|          |..|
Zfish   908 -----------------DSESSDEEDAKATLNDGDSSVCSTVDYQPPEPEP----------EPEE 945

  Fly  1214 GEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGD 1278
            .||                ||.|.||                    |..|..:...::...|:.|
Zfish   946 VEE----------------EEPEPEE--------------------PEACFTEGCIRRCACLSVD 974

  Fly  1279 DDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFT 1343
            ....:.:.|.|||...|.::|:.||||.:|.|||:||.|||.||:::.:|.:::.||.|.|::||
Zfish   975 ITEGWGKKWWNLRRTCFTIVEHDYFETFIIFMILLSSGALAFEDINIERRRVIKTILEYADKVFT 1039

  Fly  1344 VIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLR 1408
            .||.:|||:||:|.|||.|||||||||||:||.|||::..|:|:|...:.|.|::||||||||||
Zfish  1040 YIFIVEMLLKWVAYGFKTYFTNAWCWLDFLIVDVSLVSLTANLMGYSELGAIKSLRTLRALRPLR 1104

  Fly  1409 AMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGT---KLSHE 1470
            |:||.:||||||||||.|||||||||||||||||||:||||.|||||::.|  :|.|   ::..:
Zfish  1105 ALSRFEGMRVVVNALVGAIPSIFNVLLVCLIFWLIFSIMGVNLFAGKFYHC--INTTTEERIPMD 1167

  Fly  1471 IIPNRNACESENYT----WVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRE 1531
            ::.|::.|.:..||    |||..:|:|:||..||.|.|:||||||:.||..|:||||||:||..|
Zfish  1168 VVNNKSDCMALMYTNEVRWVNVKVNYDNVGLGYLSLLQIATFKGWMDIMYAAVDSREVDEQPSYE 1232

  Fly  1532 TNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKK 1596
            .|:|||||||.||||||||||||||||||||||:||.|.||. ::||||:||||||||||:|:||
Zfish  1233 INLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKSKFGGK-DIFMTEEQKKYYNAMKKLGAKK 1296

  Fly  1597 PLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVI 1661
            ..|.||||....|.:||:.::.:.|||.||:.|.|||.||.::..|.|.....||..:|.:|:|:
Zfish  1297 RPKPIPRPSNIIQGLVFDFISKQFFDIFIMVLICLNMVTMMIETDDQSAEKEYVLYQINLVFIVV 1361

  Fly  1662 FSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLR 1726
            |:|||:||:||||.::|...||:||.|||||||.||:||||||||||||||.||:|:|::|||||
Zfish  1362 FTSECVLKLFALRQYFFTIGWNVFDFVVVILSIAGLMLSDIIEKYFVSPTLFRVIRLARIGRVLR 1426

  Fly  1727 LVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQS 1791
            |::||||||||||||.|||||||||.|||||:||||:|||||.|.:||:::||:|::||:|||.|
Zfish  1427 LIRGAKGIRTLLFALMMSLPALFNIGLLLFLIMFIFSIFGMSNFAYVKKQAGIDDIFNFETFGGS 1491

  Fly  1792 MILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYP-------GNCGSATVGITFLLSYLVISFL 1849
            :|.||:::|||||||:|..|:|.   .|||.|..:.       ||||:..:||.|..||:::|||
Zfish  1492 IICLFEITTSAGWDGLLLPILNS---GPPDCDPDFENPGTDVRGNCGNPGMGIMFFCSYIIMSFL 1553

  Fly  1850 IVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQ 1914
            :|:|||||:||||::.|.|:..:.|.:||:||:.|.|::||.:.||:|.||:|.:|:|.|:.||:
Zfish  1554 VVVNMYIAIILENFNNAQEESGDPLCEDDFDMFDETWEKFDVDATQFIEYDRLFDFVDALQEPLR 1618

  Fly  1915 IHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIE-----ETGEIGEIAARPDTEG 1974
            |.|||:.|:|||||||..||.::..|||.|:|::..   |:.||     |:.|...|...|.:..
Zfish  1619 IAKPNRLKLISMDIPIVNGDKIHSQDILLAVTREVL---GDTIEMDAMKESIEAKFIMNNPTSAS 1680

  Fly  1975 YEPVSSTLWRQREEYCARLIQHAWRKH 2001
            :||:.:||.|:.||..|..:|..:|:|
Zfish  1681 FEPIITTLRRKEEERAAIAVQRIYRRH 1707

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
para	NP_001259619.1	Ion_trans	152..436	CDD:459842	175/305 (57%)
		Na_trans_cytopl	555..740	CDD:463401	22/184 (12%)
		Ion_trans	817..1028	CDD:459842	139/212 (66%)
		Na_trans_assoc	1054..1296	CDD:461936	47/252 (19%)
		Ion_trans	1300..1571	CDD:459842	174/277 (63%)
		Na_channel_gate	1561..1613	CDD:240441	32/51 (63%)
		Ion_trans	1621..1871	CDD:459842	154/256 (60%)
		GPHH	1882..1929	CDD:465306	24/46 (52%)
scn4aa	NP_001034914.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	32..52		7/38 (18%)
		I. /evidence=ECO:0000305	106..421		183/323 (57%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	446..468		6/90 (7%)
		II. /evidence=ECO:0000305	564..836		169/271 (62%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	901..957		18/132 (14%)
		III. /evidence=ECO:0000305	979..1292		195/315 (62%)
		Important for rapid channel inactivation. /evidence=ECO:0000250|UniProtKB:P15390	1276..1278		0/1 (0%)
		IV. /evidence=ECO:0000305	1301..1599		170/300 (57%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1765..1786