Protein Alignment para and scn8a

DIOPT Version :9

• Sequence 1: NP_001259619.1
• Gene: para / 32619
• FlyBaseID: FBgn0285944
• Length: 2145
• Species: Drosophila melanogaster
• Sequence 2: XP_031751845.1
• Gene: scn8a / 549640
• XenbaseID: XB-GENE-1011299
• Length: 1987
• Species: Xenopus tropicalis
• Alignment Length: 2146
• Identity: 965/2146 - (44%)
• Similarity: 1298/2146 - (60%)
• Gaps: 322/2146 - (15%)

  Fly    16 FRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLE 80
            |:.||||||..||:|||.|..|                 :..||.....||:||:: |:|:..||
 Frog    14 FKAFTRESLAAIEKRIAEEKNK-----------------KPPKQDSSHRDDDDENK-PRPNSDLE 60

  Fly    81 QGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRV 145
            .|..:|. :.|..|..|.:.|:||:||:|.:..||:|:::||.||||||:.|:::|.||||:|:.
 Frog    61 AGKGVPF-IYGDCPSRLIAEPIEDLDPFYLHTKTFIVLNRGKTIFRFSAAPALYILSPFNPLRKF 124

  Fly   146 AIYILVHPLFSLFIITTILVNCILMIMPTTPT-VESTEVIFTGIYTFESAVKVMARGFILCPFTY 209
            ||.||:|.|||:.|:.|||.||:.|.....|. .:..|..|||||||||.||::||||.:..||:
 Frog   125 AIKILIHSLFSMIIMCTILTNCVFMTFSNPPEWSKQVEYTFTGIYTFESLVKIIARGFCIDGFTF 189

  Fly   210 LRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDV 274
            |||.||||||.||.:||||..:||||::|||||||||||||::::|||||||||:|:|||.|.||
 Frog   190 LRDPWNWLDFSVIMMAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDV 254

  Fly   275 IILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLD--------GSWGNLTDENWDYHNRNSSNWYS 331
            :|||:|.||||||:|||::||.|.:||: .:|:|        ||.|    .::|.:..|.:|::.
 Frog   255 MILTVFCLSVFALIGLQLFMGHLRRKCV-AWPVDMDQMYLANGSKG----FDFDEYIMNDTNFFK 314

  Fly   332 EDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQL 396
            ....|...||||.|.||||.:.|.|::. |.||||||||||:|.||||:.||||||||||:||||
 Frog   315 VHGAIDPLLCGNGSDAGQCPEGYTCMKA-GRNPNYGYTSFDTFSWAFLALFRLMTQDFWENLYQL 378

  Fly   397 VLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEE--AAAA 459
            .|||:|..:|:||:::||:|||||||||||:|||:|:      |:.:|..|||.|:..|  |...
 Frog   379 TLRASGKTYMIFFVLVIFVGSFYLVNLILAVVAMAYE------EQNQATLEEAERKENEFKAMLE 437

  Fly   460 KAAKLEERANAQAQAAADAAAAEEAA-----------LHPEMAKSPTYSC----------ISYEL 503
            :..:::|.|.|.|.|.:.....|:|.           ...|.:|..:.|.          ...||
 Frog   438 QMKRMQEEAQAAANATSAGTVTEDAVEDEDGEARMSHTSSEFSKPSSKSAKERRNRRKKRKQKEL 502

  Fly   504 FVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTS-LSLPGSPFNIR 567
            ..|.|||    :.||:      .:|||...::|:......::..:...:...| ||:||||:..|
 Frog   503 SEGEEKG----DSEKV------YKSESEDGVKRRGFWYPDNRLGRRFSIMNQSLLSIPGSPYLSR 557

  Fly   568 RGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSE-ENGAIIVPVY 631
            ..|:||    |.:.||||..|.|:.:               :|||.::....|| ...::.:||
 Frog   558 HNSKSS----IFSFRGRFRDPVSENE---------------FADDEHSTVEESEGRRDSLFIPV- 602

  Fly   632 YGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESK----LRNRNTRNQSV------- 685
                       ..|..|.||.|           |.|..::.|:    ||....||.:|
 Frog   603 -----------RGHDRRNSYNS-----------GYSQGSRSSRILQNLRRNPKRNSTVDCNGVVS 645

  Fly   686 -----GATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMK-----D 740
                 |:...|...|.        :.:|....||        |:|       .|.|::|     .
 Frog   646 LIGGPGSNIPGGRLLP--------EVKIDKAATD--------DSP-------TTEVEVKRKPSGS 687

  Fly   741 VMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWL 805
            :.|..|.:..:.||     .|..|:....|:...|:....:.|......|..|.:.:|.||.:|:
 Frog   688 LRVSMDQLTASFGR-----KRANSILSVVTQTLVEELEESQRKCPPCWYKFADTYLIWTCCPLWI 747

  Fly   806 KFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLM 870
            :.:..|::||.||||:|.||:|||:||:||||:|:.|....|.||..||..||..|..|..:||:
 Frog   748 RIKTIVNMIVMDPFVDLTITICIVLNTLFMAMEHYPMTPHFENVLVVGNLVFTGIFTAEMFLKLI 812

  Fly   871 AMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGR 935
            ||.|.|||||||||||..||:|||:||||:.|:||||||||||||||||||||||||:||.|:|.
 Frog   813 AMDPYYYFQEGWNIFDGFIVSLSLMELGLQDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGN 877

  Fly   936 TMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRF-PDGDLPRWNFTDFMHSFMIVFRVLCG 999
            ::|||||||.||.||:|||||:|||||||:|.|...:. |:..||||:..||.|||:||||||||
 Frog   878 SVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKINPECVLPRWHMNDFFHSFLIVFRVLCG 942

  Fly  1000 EWIESMWDCMYV-GDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEA 1063
            ||||:|||||.| |...||..|:..:|||||||||||||||||:|.:.:|:|...|.:.|.:..:
 Frog   943 EWIETMWDCMEVAGQAMCIIVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDGEMNNLQIS 1007

  Fly  1064 FNRIGRFKSWVKRNIADCF-KLIRNKLTNQIS--DQPSGERTNQISWIWSEGKGVCRCISAEHGD 1125
            ..||.:..:|:|....|.. |..:.|..:::.  |:...::.|              |::...|
 Frog  1008 VIRIKKGIAWIKLKAHDFMQKHFKKKDADEVKPLDEMYEKKMN--------------CLANNTG- 1057

  Fly  1126 NELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASIN 1190
                       ||                        ||.||  :.||..   |..|....:|:.
 Frog  1058 -----------AD------------------------IHRDM--DYPKNG---NGTTSGIGSSVE 1082

  Fly  1191 SYGSHKNRPFKDESH------------------KGSAETMEGEEKRDASKEDLGLDEELDE---- 1233
            .|       ..||.|                  :...|.:..|:...||.|: |..::||:
 Frog  1083 KY-------MIDEDHMSFIHNPNLTVKVPIAVGESDFENLNTEDFSSASDEE-GSKDKLDDTSSS 1139

  Fly  1234 EG-------ECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLR 1291
            ||       |.||.|::       ..|:.||  |.:|..:....:|.......:....:.|..||
 Frog  1140 EGSTIDIKPEAEEVPVE-------QPEEYLD--PDNCFTEGCVARFKCCQVSVEEGLGKSWWTLR 1195

  Fly  1292 LKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLA 1356
            ...|.::|:.:|||.:|.|||:||.|||.|||::.||..::.||.|.|::||.||.||||:||||
 Frog  1196 KTCFLIVEHNWFETFIIFMILLSSGALAFEDVYIEQRKTIRTILEYADKVFTYIFILEMLLKWLA 1260

  Fly  1357 LGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVN 1421
            .||..:||||||||||:||.|||::.:|:.:|...:.|.|::|||||||||||:||.:|||||||
 Frog  1261 YGFVKFFTNAWCWLDFLIVDVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVN 1325

  Fly  1422 ALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKC-----------EDMNGTKLSHEIIPNR 1475
            |||.|||||.|||||||||||||:||||.||||||:.|           ||:.......::|.|
 Frog  1326 ALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYYCYNETAEGMFEIEDVENETQCFDLIGN- 1389

  Fly  1476 NACESENYT---WVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMY 1537
                  |||   |.|..:|||:||..||.|.||||||||:.||..|:|||:|.:||..|.|||||
 Frog  1390 ------NYTEVRWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRKVHQQPKYEDNIYMY 1448

  Fly  1538 LYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIP 1602
            :|||.||||||||||||||||||||||:||||.||. ::||||:||||||||||:|||||.|.||
 Frog  1449 IYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIP 1512

  Fly  1603 RPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECL 1667
            ||..:.|..:|:|:|.:.|||:||:.|.|||.||.::..|.||..:.||.::|.:|:|.|::||:
 Frog  1513 RPLNKFQGAIFDIITQQAFDIVIMILICLNMVTMMVETDDQSDYTDNVLYWINVVFIVFFTTECV 1577

  Fly  1668 LKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAK 1732
            ||:.|||::||...||:||.|||||||:|:.|:|:|.||||||||.||:|:|::||:|||:||||
 Frog  1578 LKLCALRHYYFTIGWNIFDFVVVILSIVGMFLADLITKYFVSPTLFRVIRLARIGRILRLIKGAK 1642

  Fly  1733 GIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQ 1797
            |||||||||.|||||||||.||||||||||:|||||.|.:||:::||:|::||:|||.|||.|||
 Frog  1643 GIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYVKKEAGIDDMFNFETFGNSMICLFQ 1707

  Fly  1798 MSTSAGWDGVLDAIINEEACDPPDND--KGYP-----GNCGSATVGITFLLSYLVISFLIVINMY 1855
            ::|||||||:|..|:|.    |||.|  |..|     |:||:.:|||.|.:||::||||||:|||
 Frog  1708 ITTSAGWDGLLLPILNR----PPDCDAHKENPGSQVKGDCGNPSVGIFFFVSYIIISFLIVVNMY 1768

  Fly  1856 IAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNK 1920
            ||:||||:|.|||:..:.|::||::.:||||::|||:.||:|::.:|.:|.|.||.||:|.|||.
 Frog  1769 IAIILENFSVATEESADPLSEDDFETFYEIWEKFDPDATQFIQFSKLPDFADALEHPLRIPKPNT 1833

  Fly  1921 YKIISMDIPICRGDLMYCVDILDALTKDFFARKG--NPIEETGEIGEIAARPDTEGYEPVSSTLW 1983
            .::|:||:|:..||.::|:|||.|.||......|  :.:.:..|...:|:.|....|||:::||.
 Frog  1834 IELIAMDLPMVSGDRIHCLDILFAFTKRVLGESGELDILRQQMEERFVASNPSKVSYEPITTTLR 1898

  Fly  1984 RQREEYCARLIQHAWRKHKA-RG-----------EGGGSFEPDTDHGDGGDPDAGDPAPDEATDG 2036
            |::|:..|.:||.|:|:|.. ||           |.||:   :.|..:|....|..|:.|..|..
 Frog  1899 RKQEDVSAVVIQQAYRRHLVKRGFISRKRSTGKMENGGT---NMDKKEGTPSTASLPSYDSVTKP 1960

  Fly  2037 D 2037
            |
 Frog  1961 D 1961

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
para	NP_001259619.1	Ion_trans	165..436	CDD:278921	162/279 (58%)
		Na_trans_cytopl	515..648	CDD:288761	32/134 (24%)
		Ion_trans	835..1028	CDD:278921	127/194 (65%)
		Na_trans_assoc	1054..1296	CDD:284034	51/273 (19%)
		Ion_trans	1318..1571	CDD:278921	165/266 (62%)
		Na_channel_gate	1561..1613	CDD:240441	35/51 (69%)
		Ion_trans	1636..1871	CDD:278921	149/241 (62%)
		GPHH	1880..1930	CDD:293510	24/49 (49%)
scn8a	XP_031751845.1	Ion_trans	131..421	CDD:395416	171/301 (57%)
		Na_trans_cytopl	503..709	CDD:403218	65/285 (23%)
		Ion_trans	759..972	CDD:395416	140/212 (66%)
		Na_trans_assoc	998..1199	CDD:399489	51/272 (19%)
		Ion_trans	1204..1480	CDD:395416	173/282 (61%)
		Na_channel_gate	1472..1524	CDD:240441	35/52 (67%)
		Ion_trans	1529..1783	CDD:395416	159/257 (62%)
		GPHH	1795..1842	CDD:407139	24/46 (52%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	345	1.000	Domainoid score	I1047
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1626	1.000	Inparanoid score	I77
OMA	1	1.010	-	-		QHG52281
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000268
OrthoInspector	1	1.000	-	-		otm48939
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R175
SonicParanoid	1	1.000	-	-		X214
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	9	9.000