DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment para and Scn8a

DIOPT Version :9

Sequence 1:	NP_001259619.1	Gene:	para / 32619	FlyBaseID:	FBgn0285944	Length:	2145	Species:	Drosophila melanogaster
Sequence 2:	NP_062139.2	Gene:	Scn8a / 29710	RGDID:	3638	Length:	1978	Species:	Rattus norvegicus

Alignment Length:	2119	Identity:	951/2119 - (44%)
Similarity:	1281/2119 - (60%)	Gaps:	277/2119 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly    16 FRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLE 80
            |:|||.|||..||:|||    :.|..:..:|:|.              :.::|||..|:|:..||
  Rat    14 FKPFTPESLANIERRIA----ESKLKKPPKADGS--------------HREDDEDSKPKPNSDLE 60

  Fly    81 QGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRV 145
            .|..:|. :.|..|..|.:.||||.||||....||||:::||.:|||||:.|:::|.|||.|||:
  Rat    61 AGKSLPF-IYGDIPQGLVAVPLEDFDPYYLTQKTFVVLNRGKTLFRFSATPALYILSPFNLIRRI 124

  Fly   146 AIYILVHPLFSLFIITTILVNCILMIMPTTPT-VESTEVIFTGIYTFESAVKVMARGFILCPFTY 209
            ||.||:|.:||:.|:.|||.||:.|.....|. .::.|..|||||||||.||::||||.:..||:
  Rat   125 AIKILIHSVFSMIIMCTILTNCVFMTFSNPPEWSKNVEYTFTGIYTFESLVKIIARGFCIDGFTF 189

  Fly   210 LRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDV 274
            |||.||||||.||.:||||..:||||::|||||||||||||::::|||||||||:|:|||.|.||
  Rat   190 LRDPWNWLDFSVIMMAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDV 254

  Fly   275 IILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDEN------WDYHNRNSSNWYSED 333
            :|||:|.||||||:|||::||.|..||: .:|::.:...|  ||      |:.:..|.:|:|...
  Rat   255 MILTVFCLSVFALIGLQLFMGNLRNKCV-VWPINFNESYL--ENGTRGFDWEEYINNKTNFYMVP 316

  Fly   334 EGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVL 398
            ..:...||||.|.||||.:.:.|::. |.||||||||||:|.||||:.|||||||:||:||||.|
  Rat   317 GMLEPLLCGNSSDAGQCPEGFQCMKA-GRNPNYGYTSFDTFSWAFLALFRLMTQDYWENLYQLTL 380

  Fly   399 RAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAK 463
            ||||..:|:||:::||:|||||||||||:|||:|:| |.:|..|||.::||   ..:|...:..|
  Rat   381 RAAGKTYMIFFVLVIFVGSFYLVNLILAVVAMAYEE-QNQATLEEAEQKEA---EFKAMLEQLKK 441

  Fly   464 LEERANAQAQAAADAAAAEEA------------ALHPEMAKSPTYSC----------ISYELFVG 506
            .:|.|.|.|.|.:....:|:|            ....|::|..:.|.          ...||..|
  Rat   442 QQEEAQAAAMATSAGTVSEDAIEEEGEDGVGSPRSSSELSKLSSKSAKERRNRRKKRKQKELSEG 506

  Fly   507 GEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQ-ATKVRKVSTTSLSLPGSPFNIRRGS 570
            .|||    :.||:      .:|||...::|:......:: ..|...::.:.||:|||||..|..|
  Rat   507 EEKG----DPEKV------FKSESEDGMRRKAFRLPDNRIGRKFSIMNQSLLSIPGSPFLSRHNS 561

  Fly   571 RSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSE-ENGAIIVPVYYGN 634
            :|| .::.| |.|||..|||:.:               :|||.::....|| ...::.:|:   .
  Rat   562 KSS-IFSFR-GPGRFRDPGSENE---------------FADDEHSTVEESEGRRDSLFIPI---R 606

  Fly   635 LGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESK----LRNRNTRNQSVGATNGGTTCL 695
            ...|.|||:.:.                  |.|..::.|:    ||....||.:|.. ||..:.:
  Rat   607 ARERRSSYSGYS------------------GYSQCSRSSRIFPSLRRSVKRNSTVDC-NGVVSLI 652

  Fly   696 DTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAA--GRHSRA 758
            ...                      .|....:....|:..:..|....|...::|.|  ||
  Rat   653 GPG----------------------SHIGRLLPEATTEVEIKKKGPGSLLVSMDQLASYGR---- 691

  Fly   759 SDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELF 823
            .||..|:....|....|:....:.|......|..:.|.:|:|...|:|.:|.|:|||.||||:|.
  Rat   692 KDRINSIMSVVTNTLVEELEESQRKCPPCWYKFANTFLIWECHPYWIKLKEIVNLIVMDPFVDLA 756

  Fly   824 ITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFI 888
            ||:|||:||:||||:||.|..:.|.||..||..||..|..|..:||:||.|.|||||||||||..
  Rat   757 ITICIVLNTLFMAMEHHPMTPQFEHVLAVGNLVFTGIFTAEMFLKLIAMDPYYYFQEGWNIFDGF 821

  Fly   889 IVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFI 953
            ||:|||:||.|..|:||||||||||||||||||||||||:||.|:|.::|||||||.||.||:||
  Rat   822 IVSLSLMELSLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFI 886

  Fly   954 FAVMGMQLFGKNYHDHKDRF-PDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYV-GDVSC 1016
            |||:|||||||:|.:...:. .:..||||:..||.|||:||||||||||||:|||||.| |...|
  Rat   887 FAVVGMQLFGKSYKECVCKINQECKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMC 951

  Fly  1017 IPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADC 1081
            :..|:..:|||||||||||||||||:|.:.:|:|...|.:.|.:..:..||.:..:|.|..: ..
  Rat   952 LIVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDGEMNNLQISVIRIKKGVAWTKVKV-HA 1015

  Fly  1082 FKLIRNKLTNQISDQPSGERTNQISWIWSEGKGVCRCISAEHGDNELELGHDEILADGLIKKGIK 1146
            |.....|       |...:....:..:: |.|..|
  Rat  1016 FMQAHFK-------QREADEVKPLDELY-EKKANC------------------------------ 1042

  Fly  1147 EQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKG---- 1207
                  :|...|::...:||.:.|.       |..|....:|:..|       ..||.|..
  Rat  1043 ------IANHTGVDIHRNGDFQKNG-------NGTTSGIGSSVEKY-------IIDEDHMSFINN 1087

  Fly  1208 -------------------SAETMEGEEKRDASKEDLGLDEELDEEG-------ECEEGPLDGDI 1246
                               :.|.:..|...:.||:.  ||:....||       |.||.|::
  Rat  1088 PNLTVRVPIAVGESDFENLNTEDVSSESDPEGSKDK--LDDTSSSEGSTIDIKPEVEEVPVE--- 1147

  Fly  1247 IIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMI 1311
                ..|:.||  |..|..:...::|.....:.:....:.|..||...|.::|:.:|||.:|.||
  Rat  1148 ----QPEEYLD--PDACFTEGCVQRFKCCQVNIEEGLGKSWWILRKTCFLIVEHNWFETFIIFMI 1206

  Fly  1312 LMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVM 1376
            |:||.|||.||:::.||..::.||.|.|::||.||.||||:||.|.||..:||||||||||:||.
  Rat  1207 LLSSGALAFEDIYIEQRKTIRTILEYADKVFTYIFILEMLLKWTAYGFVKFFTNAWCWLDFLIVA 1271

  Fly  1377 VSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFW 1441
            |||::.:|:.:|...:.|.|::|||||||||||:||.:||||||||||.|||||.||||||||||
  Rat  1272 VSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFW 1336

  Fly  1442 LIFAIMGVQLFAGKYFKC-EDMNGTKLSHEIIPNRNACE------SENYTWVNSAMNFDHVGNAY 1499
            |||:||||.||||||..| .:.:..:...:|:.|:..||      |....|.|..:|||:||..|
  Rat  1337 LIFSIMGVNLFAGKYHYCFNETSEIRFEIDIVNNKTDCEKLMEGNSTEIRWKNVKINFDNVGAGY 1401

  Fly  1500 LCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFN 1564
            |.|.||||||||:.||..|:|||:.|:||..|.|||||:|||.||||||||||||||||||||||
  Rat  1402 LALLQVATFKGWMDIMYAAVDSRKPDEQPDYEGNIYMYIYFVIFIIFGSFFTLNLFIGVIIDNFN 1466

  Fly  1565 EQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFI 1629
            :||||.||. ::||||:||||||||||:|||||.|.||||..:.|.|||:.||.:.|||:||:.|
  Rat  1467 QQKKKFGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKIQGIVFDFVTQQAFDIVIMMLI 1530

  Fly  1630 GLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSI 1694
            .|||.||.::....|.....:|.::|.:||:.|:.||:||:||||::||...||:||.|||||||
  Rat  1531 CLNMVTMMVETDTQSKQMENILYWINLVFVIFFTCECVLKMFALRHYYFTIGWNIFDFVVVILSI 1595

  Fly  1695 LGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVM 1759
            :|:.|:||||||||||||.||:|:|::||:|||:|||||||||||||.|||||||||.|||||||
  Rat  1596 VGMFLADIIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVM 1660

  Fly  1760 FIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPD--N 1822
            |||:|||||.|.:||.::||:|::||:|||.|||.|||::|||||||:|..|:|.    |||  .
  Rat  1661 FIFSIFGMSNFAYVKHEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLLPILNR----PPDCSL 1721

  Fly  1823 DK-----GYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMY 1882
            ||     |:.|:||:.:|||.|.:||::||||||:|||||:||||:|.|||:..:.|::||::.:
  Rat  1722 DKEHPGSGFKGDCGNPSVGIFFFVSYIIISFLIVVNMYIAIILENFSVATEESADPLSEDDFETF 1786

  Fly  1883 YEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTK 1947
            ||||::|||:.||:|.|.:|::|.|.||.||::.|||..::|:||:|:..||.::|:|||.|.||
  Rat  1787 YEIWEKFDPDATQFIEYCKLADFADALEHPLRVPKPNTIELIAMDLPMVSGDRIHCLDILFAFTK 1851

  Fly  1948 DFFARKG--NPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARG----- 2005
            ......|  :.:.:..|...:|:.|....|||:::||.|::||..|.::|.|:|.|.||.
  Rat  1852 RVLGDSGELDILRQQMEERFVASNPSKVSYEPITTTLRRKQEEVSAVVLQRAYRGHLARRGFICR 1916

  Fly  2006 -------EGGGSFEPDTDHGDGGDPDAGDPAPDEATDGD 2037
                   |.||:..   |..:.....|..|:.|..|..|
  Rat  1917 KMASNKLENGGTHR---DKKESTPSTASLPSYDSVTKPD 1952

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
para	NP_001259619.1	Ion_trans	165..436	CDD:278921	161/277 (58%)
		Na_trans_cytopl	515..648	CDD:288761	36/134 (27%)
		Ion_trans	835..1028	CDD:278921	124/194 (64%)
		Na_trans_assoc	1054..1296	CDD:284034	45/271 (17%)
		Ion_trans	1318..1571	CDD:278921	161/259 (62%)
		Na_channel_gate	1561..1613	CDD:240441	36/51 (71%)
		Ion_trans	1636..1871	CDD:278921	149/241 (62%)
		GPHH	1880..1930	CDD:293510	24/49 (49%)
Scn8a	NP_062139.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..20		4/5 (80%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	28..62		13/51 (25%)
		I. /evidence=ECO:0000305	114..442		187/335 (56%)
		Ion_trans	131..421	CDD:395416	169/294 (57%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	446..530		21/93 (23%)
		Na_trans_cytopl	504..700	CDD:403218	61/270 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	576..597		8/35 (23%)
		II. /evidence=ECO:0000305	733..1005		167/271 (62%)
		Ion_trans	750..963	CDD:395416	137/212 (65%)
		Na_trans_assoc	989..1190	CDD:399489	45/270 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1105..1146		11/42 (26%)
		III. /evidence=ECO:0000305	1178..1493		191/315 (61%)
		Ion_trans	1195..1471	CDD:395416	169/275 (61%)
		Na_channel_gate	1463..1515	CDD:240441	37/52 (71%)
		IV. /evidence=ECO:0000305	1502..1799		179/300 (60%)
		Ion_trans	1520..1774	CDD:395416	159/257 (62%)
		GPHH	1786..1833	CDD:407139	24/46 (52%)
		IQ	1892..1913	CDD:197470	9/20 (45%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1923..1978		9/33 (27%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	342	1.000	Domainoid score	I1036
eggNOG	1	0.900	-	-		E1_KOG2301
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1645	1.000	Inparanoid score	I74
OMA	1	1.010	-	-		QHG52281
OrthoDB	1	1.010	-	-		D37221at33208
OrthoFinder	1	1.000	-	-		FOG0000268
OrthoInspector	1	1.000	-	-		otm45846
orthoMCL	1	0.900	-	-		OOG6_100419
Panther	1	1.100	-	-	O	PTHR10037
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X214
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	13	12.880