DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment para and Scn8a

DIOPT Version :9

Sequence 1:	NP_001259619.1	Gene:	para / 32619	FlyBaseID:	FBgn0285944	Length:	2145	Species:	Drosophila melanogaster
Sequence 2:	XP_017172001.1	Gene:	Scn8a / 20273	MGIID:	103169	Length:	1988	Species:	Mus musculus

Alignment Length:	2129	Identity:	959/2129 - (45%)
Similarity:	1289/2129 - (60%)	Gaps:	287/2129 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly    16 FRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLE 80
            |:|||.|||..||:|||    :.|..:..:|:|.              :.::|||..|:|:..||
Mouse    14 FKPFTPESLANIERRIA----ESKLKKPPKADGS--------------HREDDEDSKPKPNSDLE 60

  Fly    81 QGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRV 145
            .|..:|. :.|..|..|.:.||||.||||....||||:::||.:|||||:.|:::|.|||.|||:
Mouse    61 AGKSLPF-IYGDIPQGLVAVPLEDFDPYYLTQKTFVVLNRGKTLFRFSATPALYILSPFNLIRRI 124

  Fly   146 AIYILVHPLFSLFIITTILVNCILMIMPTTPT-VESTEVIFTGIYTFESAVKVMARGFILCPFTY 209
            ||.||:|.:||:.|:.|||.||:.|.....|. .::.|..|||||||||.||::||||.:..||:
Mouse   125 AIKILIHSVFSMIIMCTILTNCVFMTFSNPPEWSKNVEYTFTGIYTFESLVKIIARGFCIDGFTF 189

  Fly   210 LRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDV 274
            |||.||||||.||.:||||..:||||::|||||||||||||::::|||||||||:|:|||.|.||
Mouse   190 LRDPWNWLDFSVIMMAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDV 254

  Fly   275 IILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDEN------WDYHNRNSSNWYSED 333
            :|||:|.||||||:|||::||.|..||: .:|::.:...|  ||      |:.:..|.:|:|...
Mouse   255 MILTVFCLSVFALIGLQLFMGNLRNKCV-VWPINFNESYL--ENGTRGFDWEEYINNKTNFYMVP 316

  Fly   334 EGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVL 398
            ..:...||||.|.||||.:.:.|::. |.||||||||||:|.||||:.|||||||:||:||||.|
Mouse   317 GMLEPLLCGNSSDAGQCPEGFQCMKA-GRNPNYGYTSFDTFSWAFLALFRLMTQDYWENLYQLTL 380

  Fly   399 RAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAK 463
            ||||..:|:||:::||:|||||||||||:|||:|:| |.:|..|||.::||   ..:|...:..|
Mouse   381 RAAGKTYMIFFVLVIFVGSFYLVNLILAVVAMAYEE-QNQATLEEAEQKEA---EFKAMLEQLKK 441

  Fly   464 LEERANAQAQAAADAAAAEEA------------ALHPEMAKSPTYSC----------ISYELFVG 506
            .:|.|.|.|.|.:....:|:|            ....|::|..:.|.          ...||..|
Mouse   442 QQEEAQAAAMATSAGTVSEDAIEEEGEDGVGSPRSSSELSKLSSKSAKERRNRRKKRKQKELSEG 506

  Fly   507 GEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQ-ATKVRKVSTTSLSLPGSPFNIRRGS 570
            .|||    :.||:      .:|||...::|:......:: ..|...::.:.||:|||||..|..|
Mouse   507 EEKG----DPEKV------FKSESEDGMRRKAFRLPDNRIGRKFSIMNQSLLSIPGSPFLSRHNS 561

  Fly   571 RSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSE-ENGAIIVPVYYGN 634
            :|| .::.| |.|||..|||:.:               :|||.::....|| ...::.:|:   .
Mouse   562 KSS-IFSFR-GPGRFRDPGSENE---------------FADDEHSTVEESEGRRDSLFIPI---R 606

  Fly   635 LGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESK----LRNRNTRNQSVGATNGGTTCL 695
            ...|.|||:.:.                  |.|..::.|:    ||....||.:|.. ||..:.:
Mouse   607 ARERRSSYSGYS------------------GYSQCSRSSRIFPSLRRSVKRNSTVDC-NGVVSLI 652

  Fly   696 DTNHKLDHRDYEIGLE--CTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAA--GRHS 756
            .....:.....|:.::  .||.|              .|:..:..|....|...:||.|  ||
Mouse   653 GPGSHIGRLLPEVKIDKAATDSA--------------TTEVEIKKKGPGSLLVSMEQLASYGR-- 701

  Fly   757 RASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVE 821
              .||..|:....|....|:....:.|......|..:.|.:|:|...|:|.:|.|:|||.||||:
Mouse   702 --KDRINSIMSVVTNTLVEELEESQRKCPPCWYKFANTFLIWECHPYWIKLKEIVNLIVMDPFVD 764

  Fly   822 LFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFD 886
            |.||:|||:||:||||:||.|..:.|.||..||..||..|..|..:||:||.|.|||||||||||
Mouse   765 LAITICIVLNTLFMAMEHHPMTPQFEHVLAVGNLVFTGIFTAEMFLKLIAMDPYYYFQEGWNIFD 829

  Fly   887 FIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIII 951
            ..||:|||:||||..|:||||||||||||||||||||||||:||.|:|.::|||||||.||.||:
Mouse   830 GFIVSLSLMELGLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIV 894

  Fly   952 FIFAVMGMQLFGKNYHDHKDRF-PDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYV-GDV 1014
            |||||:|||||||:|.:...:. .:..||||:..||.|||:||||||||||||:|||||.| |..
Mouse   895 FIFAVVGMQLFGKSYKECVCKISQECKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQA 959

  Fly  1015 SCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIA 1079
            .|:..|:..:|||||||||||||||||:|.:.:|:|...|.:.|.:..:..||.:..:|.|..:
Mouse   960 MCLIVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDGEMNNLQISVIRIKKGVAWAKVKV- 1023

  Fly  1080 DCFKLIRNKLTNQISDQPSGERTNQISWIWSEGKGVCRCISAEHGDNELELGHDEILADGLIKKG 1144
            ..|.....|       |...:....:..:: |.|..|
Mouse  1024 HAFMQAHFK-------QREADEVKPLDELY-EKKANC---------------------------- 1052

  Fly  1145 IKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKG-- 1207
                    :|...|::...:||.:.|.       |..|....:|:..|       ..||.|..
Mouse  1053 --------IANHTGVDIHRNGDFQKNG-------NGTTSGIGSSVEKY-------IIDEDHMSFI 1095

  Fly  1208 ---------------------SAETMEGEEKRDASKEDLGLDEELDEEG-------ECEEGPLDG 1244
                                 :.|.:..|...:.||:.  ||:....||       |.||.|::
Mouse  1096 NNPNLTVRVPIAVGESDFENLNTEDVSSESDPEGSKDK--LDDTSSSEGSTIDIKPEVEEVPVE- 1157

  Fly  1245 DIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVIT 1309
                  ..|:.||  |..|..:...::|.....:.:....:.|..||...|.::|:.:|||.:|.
Mouse  1158 ------QPEEYLD--PDACFTEGCVQRFKCCQVNIEEGLGKSWWILRKTCFLIVEHNWFETFIIF 1214

  Fly  1310 MILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVI 1374
            |||:||.|||.||:::.||..::.||.|.|::||.||.||||:||.|.||..:||||||||||:|
Mouse  1215 MILLSSGALAFEDIYIEQRKTIRTILEYADKVFTYIFILEMLLKWTAYGFVKFFTNAWCWLDFLI 1279

  Fly  1375 VMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLI 1439
            |.|||::.:|:.:|...:.|.|::|||||||||||:||.:||||||||||.|||||.||||||||
Mouse  1280 VAVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLI 1344

  Fly  1440 FWLIFAIMGVQLFAGKYFKCEDMNGT-KLSHEI--IPNRNACE------SENYTWVNSAMNFDHV 1495
            |||||:||||.||||||..|  .|.| ::..||  :.|:..||      :....|.|..:|||:|
Mouse  1345 FWLIFSIMGVNLFAGKYHYC--FNETSEIRFEIDEVNNKTDCEKLMEGNNTEIRWKNVKINFDNV 1407

  Fly  1496 GNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVII 1560
            |..||.|.||||||||:.||..|:|||:.|:||..|.|||||:|||.||||||||||||||||||
Mouse  1408 GAGYLALLQVATFKGWMDIMYAAVDSRKPDEQPDYEGNIYMYIYFVIFIIFGSFFTLNLFIGVII 1472

  Fly  1561 DNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIII 1625
            ||||:||||.||. ::||||:||||||||||:|||||.|.||||..:.|.|||:.||.:.|||:|
Mouse  1473 DNFNQQKKKFGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKIQGIVFDFVTQQAFDIVI 1536

  Fly  1626 MLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVV 1690
            |:.|.|||.||.::....|.....:|.::|.:||:.|:.||:||:||||::||...||:||.|||
Mouse  1537 MMLICLNMVTMMVETDTQSKQMENILYWINLVFVIFFTCECVLKMFALRHYYFTIGWNIFDFVVV 1601

  Fly  1691 ILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLL 1755
            ||||:|:.|:||||||||||||.||:|:|::||:|||:|||||||||||||.|||||||||.|||
Mouse  1602 ILSIVGMFLADIIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLL 1666

  Fly  1756 FLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPP 1820
            |||||||:|||||.|.:||.::||:|::||:|||.|||.|||::|||||||:|..|:|.    ||
Mouse  1667 FLVMFIFSIFGMSNFAYVKHEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLLPILNR----PP 1727

  Fly  1821 D--NDK-----GYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDD 1878
            |  .||     |:.|:||:.:|||.|.:||::||||||:|||||:||||:|.|||:..:.|::||
Mouse  1728 DCSLDKEHPGSGFKGDCGNPSVGIFFFVSYIIISFLIVVNMYIAIILENFSVATEESADPLSEDD 1792

  Fly  1879 YDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILD 1943
            ::.:||||::|||:.||:|.|.:|::|.|.||.||::.|||..::|:||:|:..||.::|:|||.
Mouse  1793 FETFYEIWEKFDPDATQFIEYCKLADFADALEHPLRVPKPNTIELIAMDLPMVSGDRIHCLDILF 1857

  Fly  1944 ALTKDFFARKG--NPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARG- 2005
            |.||......|  :.:.:..|...:|:.|....|||:::||.|::||..|.::|.|:|.|.||.
Mouse  1858 AFTKRVLGDSGELDILRQQMEERFVASNPSKVSYEPITTTLRRKQEEVSAVVLQRAYRGHLARRG 1922

  Fly  2006 -----------EGGGSFE------PDTDHGDGGDPDAGDPAPDEATDGD 2037
                       |.||:..      |.|         |..|:.|..|..|
Mouse  1923 FICRKITSNKLENGGTHREKKESTPST---------ASLPSYDSVTKPD 1962

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
para	NP_001259619.1	Ion_trans	165..436	CDD:278921	161/277 (58%)
		Na_trans_cytopl	515..648	CDD:288761	36/134 (27%)
		Ion_trans	835..1028	CDD:278921	125/194 (64%)
		Na_trans_assoc	1054..1296	CDD:284034	45/271 (17%)
		Ion_trans	1318..1571	CDD:278921	163/261 (62%)
		Na_channel_gate	1561..1613	CDD:240441	36/51 (71%)
		Ion_trans	1636..1871	CDD:278921	149/241 (62%)
		GPHH	1880..1930	CDD:293510	24/49 (49%)
Scn8a	XP_017172001.1	Ion_trans	131..421	CDD:366146	169/294 (57%)
		Na_trans_cytopl	504..710	CDD:371806	65/272 (24%)
		Ion_trans	760..973	CDD:366146	138/212 (65%)
		Na_trans_assoc	999..1200	CDD:368948	45/270 (17%)
		Ion_trans	1205..1481	CDD:366146	171/277 (62%)
		Na_channel_gate	1473..1525	CDD:240441	37/52 (71%)
		Ion_trans	1530..1784	CDD:366146	159/257 (62%)
		GPHH	1796..1843	CDD:374878	24/46 (52%)
		IQ	1902..1923	CDD:197470	9/20 (45%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	346	1.000	Domainoid score	I1042
eggNOG	1	0.900	-	-		E1_KOG2301
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1639	1.000	Inparanoid score	I81
Isobase	1	0.950	-	0	Normalized mean entropy	S5486
OMA	1	1.010	-	-		QHG52281
OrthoDB	1	1.010	-	-		D37221at33208
OrthoFinder	1	1.000	-	-		FOG0000268
OrthoInspector	1	1.000	-	-		otm43765
orthoMCL	1	0.900	-	-		OOG6_100419
Panther	1	1.100	-	-	O	PTHR10037
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R175
SonicParanoid	1	1.000	-	-		X214
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	14	13.860