DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment para and Scn4a

DIOPT Version :9

Sequence 1:	NP_001259619.1	Gene:	para / 32619	FlyBaseID:	FBgn0285944	Length:	2145	Species:	Drosophila melanogaster
Sequence 2:	NP_573462.2	Gene:	Scn4a / 110880	MGIID:	98250	Length:	1841	Species:	Mus musculus

Alignment Length:	2145	Identity:	929/2145 - (43%)
Similarity:	1236/2145 - (57%)	Gaps:	416/2145 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly    17 RPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQ 81
            ||||.|||..||||..   |::..|:|             .||.||    |:.:..|:.|  ||.
Mouse    18 RPFTPESLAAIEQRAM---EEEARLQR-------------NKQMEI----EEPERKPRSD--LEA 60

  Fly    82 GVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVA 146
            |..:|: :.|..|||:...||||:|||||:..||:|::|||.||||||:.|::||.||:.:||||
Mouse    61 GKNLPL-IYGDPPPEVIGVPLEDLDPYYSDKKTFIVLNKGKAIFRFSATPALYMLSPFSIVRRVA 124

  Fly   147 IYILVHPLFSLFIITTILVNCILMIMPTTPT-VESTEVIFTGIYTFESAVKVMARGFILCPFTYL 210
            |.:|:|.|||:||:.|||.||:.|.|...|: .:..|..|||||||||.:|::||||.:..||:|
Mouse   125 IKVLIHALFSMFIMITILTNCVFMTMSNPPSWSKDVEYTFTGIYTFESLIKMLARGFCIDDFTFL 189

  Fly   211 RDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVI 275
            ||.||||||.||.:||||..:||||::|||||||||||||:.::|||||||||:|:|||.|.||:
Mouse   190 RDPWNWLDFSVITMAYVTEFVDLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVM 254

  Fly   276 ILTMFSLSVFALMGLQIYMGVLTQKCIKKFP----LDGSW-------GNLT---DENW---DYHN 323
            |||:|.||||||:|||::||.|.|||::..|    .:.:|       ||.|   ::.|   |..|
Mouse   255 ILTVFCLSVFALVGLQLFMGNLRQKCVRWPPPMNDTNTTWYGNDTWYGNDTWYGNDTWYGNDTWN 319

  Fly   324 RNSS-------NW--YSEDEGISF--------PLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSF 371
            ...|       :|  |..|||..:        .||||.|.||.|.:.|.|::. |.||||||||:
Mouse   320 SQESWVSNSTFDWEAYINDEGNFYFLEGSNDALLCGNSSDAGHCPEGYECMKA-GRNPNYGYTSY 383

  Fly   372 DSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQ 436
            |:|.||||:.|||||||:||:|:||.|||||..:|:||:||||||||||:|||||:|||:|.|..
Mouse   384 DTFSWAFLALFRLMTQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVAMAYAEQN 448

  Fly   437 KKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYS--CI 499
            :....|:..:||..::..|.......:||:...|||....:.|..:           ||:|  |
Mouse   449 EATLAEDQEKEEEFQQMLEKFKKHQEELEKAKAAQALEGGEEADGD-----------PTHSKDC- 501

  Fly   500 SYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPF 564
            :..|...||||                          .|.|:
Mouse   502 NGSLDTSGEKG--------------------------PPRPS----------------------- 517

  Fly   565 NIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVP 629

Mouse   518 ----------------------------------------------------------------- 517

  Fly   630 VYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTC 694

Mouse   518 ----------------------------------------------------------------- 517

  Fly   695 LDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRAS 759
                             |:.|:                          .::|.:|:....|.:.
Mouse   518 -----------------CSAES--------------------------AISDAMEELEEAHQKC- 538

  Fly   760 DRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFI 824
                              |.:..|....:|       :|:||..|:||:..:.|||.||||:|.|
Mouse   539 ------------------PPWWYKCAHKVL-------IWNCCAPWVKFKHIILLIVMDPFVDLGI 578

  Fly   825 TLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFII 889
            |:|||:||:||||:|:.|.:..:.||..||..||..|..|..:||:||.|..|||:||||||..|
Mouse   579 TICIVLNTLFMAMEHYPMTEHFDNVLSVGNLVFTGIFTAEMVLKLIAMDPYEYFQQGWNIFDSFI 643

  Fly   890 VALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIF 954
            |.|||:||||..||||||||||||||||||||||||||:||.|:|.::|||||||.||.||:|||
Mouse   644 VTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIF 708

  Fly   955 AVMGMQLFGKNYHDHKDRF-PDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYV-GDVSCI 1017
            ||:|||||||:|.:...:. .|..||||:..||.|||:||||:|||||||:|||||.| |...|:
Mouse   709 AVVGMQLFGKSYKECVCKIASDCSLPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCL 773

  Fly  1018 PFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCF 1082
            ..||..:|||||||||||||||||:|.:.||:|...|.:.|.:..|   |||.|..:........
Mouse   774 TVFLMVMVIGNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIA---IGRIKWGIAFAKTFLL 835

  Fly  1083 KLIRNKLTNQISD------QPSGERTNQISWIWSEGKGVCRCISAEHGDNELELGHDEILADGLI 1141
            .|:..|:.: :.|      :|.|...|..|....|.|..    ..|.|:.||:..|  ||.
Mouse   836 GLLHGKILS-LKDIMLSLGEPGGAGENGESPPEDEKKEP----PPEDGNKELKDNH--ILN---- 889

  Fly  1142 KKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESH- 1205
                      .|.:.||...:|..|..|       ::||........|.|..|....|.::|:.
Mouse   890 ----------HVGLTDGPRSSIEMDHLN-------FINNPYLTIHVPIASEESDLEMPTEEETDT 937

  Fly  1206 -------KGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADC 1263
                   |...:.:........|..|....|| |.|.:.||.| :|             |.|.:|
Mouse   938 FSEPEDIKKPLQPLYDGNSSVCSTADYKPPEE-DPEEQAEENP-EG-------------ELPEEC 987

  Fly  1264 CPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQR 1328
            ..::..|:.|.|..|......:.|..||...|:::|:.:|||.::.|||:||.|||.||:::.||
Mouse   988 FTEACVKRCPCLYVDISQGRGKMWWTLRRACFKIVEHNWFETFIVFMILLSSGALAFEDIYIEQR 1052

  Fly  1329 PILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQ 1393
            .::|.||.|.|::||.||.||||:||:|.|||||||||||||||:||.||:|:.||:.:|...:.
Mouse  1053 RVIQTILEYADKVFTYIFILEMLLKWVAYGFKVYFTNAWCWLDFLIVDVSIISLVANWLGYSELG 1117

  Fly  1394 AFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFK 1458
            ..|::|||||||||||:||.:|||||||||:.|||||.|||||||||||||:||||.|||||::.
Mouse  1118 PIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYY 1182

  Fly  1459 CEDMNGT---KLSHEIIPNRNACESENYT----WVNSAMNFDHVGNAYLCLFQVATFKGWIQIMN 1516
            |  :|.|   :....::.|::.|||..||    |:|..:|:|:||..||.|.||||||||:.||.
Mouse  1183 C--INTTTSERFDISVVNNKSECESLMYTGQVRWMNVKVNYDNVGLGYLSLLQVATFKGWMDIMY 1245

  Fly  1517 DAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTED 1581
            .|:||||.::||..|.|:|||||||.||||||||||||||||||||||:||||.||. ::||||:
Mouse  1246 AAVDSREKEEQPDYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGK-DIFMTEE 1309

  Fly  1582 QKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDT 1646
            ||||||||||:|||||.|.||||:.:.|.:|::.||.:.|||.||:.|.|||.||.::..|.|..
Mouse  1310 QKKYYNAMKKLGSKKPQKPIPRPQNKIQGMVYDFVTKQVFDISIMILICLNMVTMMVETDDQSQL 1374

  Fly  1647 YNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPT 1711
            ...:|..:|.:|:::|:.||:||:||||::||...||:||.|||||||:||.|||:|:|||||||
Mouse  1375 KVDILYNINMVFIIVFTGECVLKMFALRHYYFTIGWNIFDFVVVILSIVGLALSDLIQKYFVSPT 1439

  Fly  1712 LLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEK 1776
            |.||:|:|::||||||::||||||||||||.|||||||||.|||||||||::|||||.|.:||::
Mouse  1440 LFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKE 1504

  Fly  1777 SGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINE--EACDPPDNDKG--YPGNCGSATVGI 1837
            |||:|::||:|||.|:|.||:::|||||||:|:.|:|.  ..|||...:.|  ..|:||:.::||
Mouse  1505 SGIDDMFNFETFGNSIICLFEITTSAGWDGLLNPILNSGPPDCDPTLENPGTNIKGDCGNPSIGI 1569

  Fly  1838 TFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQL 1902
            .|..||::||||||:|||||:||||::.|||:..|.|.:||::|:||.|::|||:.||:|.|.:|
Mouse  1570 CFFCSYIIISFLIVVNMYIAIILENFNVATEESSEPLCEDDFEMFYETWEKFDPDATQFIDYSRL 1634

  Fly  1903 SEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKG--NPIEETGEIGE 1965
            |:|:|.|:.||:|.||||.|:|::|:|:..||.::|:|||.||||:.....|  :.:::|.|...
Mouse  1635 SDFVDTLQEPLKIAKPNKIKLITLDLPMVPGDKIHCLDILFALTKEVLGDSGEMDALKQTMEEKF 1699

  Fly  1966 IAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKH-KARGEGGGSFEPDTDHGDGGDPDAGDPA 2029
            :||.|....|||:::||.|::||.||..||.|:|:| ..|.....|:.  ..|...|:   ||.|
Mouse  1700 MAANPSKVSYEPITTTLKRKQEEVCAIKIQRAYRRHLLQRSVKQASYM--YRHSQEGN---GDGA 1759

  Fly  2030 PDE----ATDGDAPAGGDGSVNGT-AEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSP 2089
            |::    |...:...|.:...||. ::|..:.|.:      |||.......|.:::....|...|
Mouse  1760 PEKEGLLANTMNKMYGSEKEDNGVQSQGEKEKDST------EDAGPTTEVTAPSSSDTALTPPPP 1818

Fly 2090 2089
Mouse 1819 1818

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
para	NP_001259619.1	Ion_trans	165..436	CDD:278921	168/305 (55%)
		Na_trans_cytopl	515..648	CDD:288761	2/132 (2%)
		Ion_trans	835..1028	CDD:278921	123/194 (63%)
		Na_trans_assoc	1054..1296	CDD:284034	57/255 (22%)
		Ion_trans	1318..1571	CDD:278921	165/259 (64%)
		Na_channel_gate	1561..1613	CDD:240441	35/51 (69%)
		Ion_trans	1636..1871	CDD:278921	143/238 (60%)
		GPHH	1880..1930	CDD:293510	26/49 (53%)
Scn4a	NP_573462.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	32..63		13/52 (25%)
		I. /evidence=ECO:0000305	113..448		187/335 (56%)
		Ion_trans	130..451	CDD:395416	177/321 (55%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	484..522		14/245 (6%)
		II. /evidence=ECO:0000305	554..826		170/274 (62%)
		Ion_trans	571..784	CDD:395416	136/212 (64%)
		Na_trans_assoc	810..1020	CDD:399489	57/255 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	854..896		14/61 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	925..983		14/72 (19%)
		III. /evidence=ECO:0000305	1007..1320		194/315 (62%)
		Ion_trans	1024..1298	CDD:395416	172/275 (63%)
		Na_channel_gate	1290..1342	CDD:240441	36/52 (69%)
		Important for rapid channel inactivation. /evidence=ECO:0000250\|UniProtKB:P15390	1304..1306		0/1 (0%)
		IV. /evidence=ECO:0000305	1329..1627		172/297 (58%)
		Ion_trans	1347..1603	CDD:395416	153/255 (60%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1776..1841		10/49 (20%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	346	1.000	Domainoid score	I1042
eggNOG	1	0.900	-	-		E1_KOG2301
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1639	1.000	Inparanoid score	I81
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG52281
OrthoDB	1	1.010	-	-		D37221at33208
OrthoFinder	1	1.000	-	-		FOG0000268
OrthoInspector	1	1.000	-	-		otm43765
orthoMCL	1	0.900	-	-		OOG6_100419
Panther	1	1.100	-	-	O	PTHR10037
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X214
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	13	12.880