DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment U2af50 and U2AF2

DIOPT Version :9

Sequence 1:	NP_001245708.1	Gene:	U2af50 / 32602	FlyBaseID:	FBgn0005411	Length:	427	Species:	Drosophila melanogaster
Sequence 2:	NP_009210.1	Gene:	U2AF2 / 11338	HGNCID:	23156	Length:	475	Species:	Homo sapiens

Alignment Length:	478	Identity:	310/478 - (64%)
Similarity:	360/478 - (75%)	Gaps:	60/478 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 NFEDKYVNIDNKDRERDRERRRH-----RSRSRDR-HRERSRDRRHH--------RNSRRKP--- 51
            :|::....::...:|||:|.|..     ||||||| .|.||||||:.        |..|.||
Human     3 DFDEFERQLNENKQERDKENRHRKRSHSRSRSRDRKRRSRSRDRRNRDQRSASRDRRRRSKPLTR 67

  Fly    52 ---------------------SLYWDVPPPGFEHITPMQYKAMQASGQIPA-----SVVPD---- 86
                                 ..||||||||||||||||||||||:|||||     ::.||
Human    68 GAKEEHGGLIRSPRHEKKKKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAV 132

  Fly    87 TPQTAVPVVGSTITRQARRLYVGNIPFGVTEEEMMEFFNQQMHLVGLAQAAGSPVLACQINLDKN 151
            || |.||||||.:|||||||||||||||:|||.||:|||.||.|.||.||.|:||||.|||.|||
Human   133 TP-TPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKN 196

  Fly   152 FAFLEFRSIDETTQAMAFDGINLKGQSLKIRRPHDYQPMPGITDTPAIKPAVVSSGVISTVVPDS 216
            ||||||||:||||||||||||..:||||||||||||||:||:::    .|:|...||:|||||||
Human   197 FAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSE----NPSVYVPGVVSTVVPDS 257

  Fly   217 PHKIFIGGLPNYLNDDQVKELLLSFGKLRAFNLVKDAATGLSKGYAFCEYVDLSITDQSIAGLNG 281
            .||:||||||||||||||||||.|||.|:|||||||:|||||||||||||||:::|||:||||||
Human   258 AHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNG 322

  Fly   282 MQLGDKKLIVQRASVGAKNA-----QNAANTTQSVMLQVPGL--SNVVTSGPPTEVLCLLNMVTP 339
            ||||||||:|||||||||||     .:..|.| .|.||||||  |.|...|.|||||||:|||.|
Human   323 MQLGDKKLLVQRASVGAKNATLVSPPSTINQT-PVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLP 386

  Fly   340 DELRDEEEYEDILEDIKEECTKYGVVRSVEIPRPIEGVEVPGCGKVFVEFNSVLDCQKAQQALTG 404
            :||.|:||||:|:||:::||:|||:|:|:|||||::|||||||||:||||.||.|||||.|.|||
Human   387 EELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVEFTSVFDCQKAMQGLTG 451

  Fly   405 RKFSDRVVVTSYFDPDKYHRREF 427
            |||::|||||.|.|||.||||:|
Human   452 RKFANRVVVTKYCDPDSYHRRDF 474

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
U2af50	NP_001245708.1	RRM1_U2AF65	103..184	CDD:240676	65/80 (81%)
		RRM2_U2AF65	218..294	CDD:240677	65/75 (87%)
		RRM3_U2AF65	328..415	CDD:240678	63/86 (73%)
U2AF2	NP_009210.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..90		24/86 (28%)
		Required for interaction with PRPF19. /evidence=ECO:0000269\|PubMed:21536736	2..93		25/89 (28%)
		Necessary and sufficient to stimulate pre-mRNAs 3'-end cleavage in a CFIm complex-dependent manner. /evidence=ECO:0000269\|PubMed:17024186	17..47		17/29 (59%)
		U2AF_lg	63..474	CDD:273727	287/416 (69%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165145395
Domainoid	1	1.000	131	1.000	Domainoid score	I5180
eggNOG	1	0.900	-	-		E1_KOG0120
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H110853
Inparanoid	1	1.050	581	1.000	Inparanoid score	I1020
Isobase	1	0.950	-	0	Normalized mean entropy	S1673
OMA	1	1.010	-	-		QHG51929
OrthoDB	1	1.010	-	-		D391481at33208
OrthoFinder	1	1.000	-	-		FOG0003153
OrthoInspector	1	1.000	-	-		oto90876
orthoMCL	1	0.900	-	-		OOG6_101356
Panther	1	1.100	-	-	LDO	PTHR23139
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1575
SonicParanoid	1	1.000	-	-		X2550
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	17	16.750