DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sl and Plcg1

DIOPT Version :9

Sequence 1:	NP_476726.2	Gene:	sl / 32601	FlyBaseID:	FBgn0003416	Length:	1236	Species:	Drosophila melanogaster
Sequence 2:	NP_037319.2	Gene:	Plcg1 / 25738	RGDID:	3347	Length:	1290	Species:	Rattus norvegicus

Alignment Length:	1285	Identity:	528/1285 - (41%)
Similarity:	762/1285 - (59%)	Gaps:	150/1285 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly     8 SAPLLGEMEQTIGMLERGTIVTKLYGKQ-RRPDRRHLMLIRETRQLLWATVATQTPRTDYEGAIQ 71
            |||...|:......||.||::|..|.|: :||:|:...:..||||:.|:..|.:     .||:|.
  Rat    15 SAPSEAEVLHLCRSLEVGTVMTLFYSKKSQRPERKTFQVKLETRQITWSRGADK-----IEGSID 74

  Fly    72 LREIREIRVGKHSKEFRLFADD-CQRFESSKCFVILHGNHFKLKSFSVVALSEIEADNWVRGLRY 135
            :|||:|||.||.|::|..:.:| ..|.:.|.|||||:|..|:||:.|:.|.||.|.:.|::||.:
  Rat    75 IREIKEIRPGKTSRDFDRYQEDPAFRPDQSHCFVILYGMEFRLKTLSLQATSEDEVNMWIKGLTW 139

  Fly   136 MVKDTLGAPYPLQIDRWLRREYYQIENVNTHSAKATEQSPAQVTIKDFKLFLAGVSCKMTTGKFM 200
            :::|||.|..||||:||||:::|.::          .....:::.||.|..|:.|:.::...:|:
  Rat   140 LMEDTLQAATPLQIERWLRKQFYSVD----------RNREDRISAKDLKNMLSQVNYRVPNMRFL 194

  Fly   201 -EHFTEDVRRKHDLKFDDFSRLYQKLLLPNGFASVLSGSGVANFPYSEDQQV---VRP------- 254
             |..|:..:|..|:.:..|::||:.|:        .|.....:.|:.|...:   .||
  Rat   195 RERLTDLEQRSGDITYGQFAQLYRSLM--------YSAQKTMDLPFLETNTLRTGERPELCQVSL 251

  Fly   255 AELKQFLETEQRDVSASEISSAAIASFIRDFVQDVERDVQEPYLTFPEFVDFLFSKQNDLWNSKY 319
            :|.:|||...|.::.|  :....:..|:..|::|..|:::|||....|.|.|||||:|.:|||:.
  Rat   252 SEFQQFLLEYQGELWA--VDRLQVQEFMLSFLRDPLREIEEPYFFLDELVTFLFSKENSVWNSQL 314

  Fly   320 DQVFMD-MNLPLSSYWIASSHNTYLTGDQFSSESSCEAYARALRMGCRCIELDCWNGPDNLPYIF 383
            |.|..: ||.|||.|||:|||||||||||||||||.|||||.|||||||||||||:|||.:|.|:
  Rat   315 DAVCPETMNNPLSHYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDGMPVIY 379

  Fly   384 HGHTITSKIKFMDVIKTIKDHAFTSSEYPVILSIEQNCSLEQQRNMAQALIEVFGDMLLTQPCDR 448
            ||||:|:||||.||:.|||:|||.:||||||||||.:||:.|||||||...:|.||.|||:|.|.
  Rat   380 HGHTLTTKIKFSDVLHTIKEHAFVASEYPVILSIEDHCSIAQQRNMAQHFRKVLGDTLLTKPVDI 444

  Fly   449 NEQHLPSPYQLRRKIILKHKKLPQ---FDDIANGISSTGSLGHRSSLGGAGGGAHGENDGENVRK 510
            ....||||.||:|||::|||||.:   ::::...:                  .:.|||   :..
  Rat   445 AADGLPSPNQLKRKILIKHKKLAEGSAYEEVPTSV------------------MYSEND---ISN 488

  Fly   511 VFKEGLLYFKDPVDKSWNLYQFVLTHQELIYSSEINESRNGNSEDDDFGLSSSCSLNSNMQQKQK 575
            ..|.|:||.:|||:..|..:.||||..::.||.| ..|..||.::::                .|
  Rat   489 SIKNGILYLEDPVNHEWYPHYFVLTSSKIYYSEE-TSSDQGNEDEEE----------------PK 536

  Fly   576 DTSANDELHFGENWFHGKL---EGGRKEADDLLKKY---KHFGDGTFLVRESATFVGDYSLSFWR 634
            :.|.:.|||..|.||||||   ..||..|:.||.:|   ....||:||||||.||||||:|||||
  Rat   537 EASGSTELHSSEKWFHGKLGAGRDGRHIAERLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWR 601

  Fly   635 RNRPNHCRIKLKHENGSIKYYLVENFVFDSLYSLIVYYRKNMLRSSEFSIILKEPVPQPKKHEDQ 699
            ..:..||||..:.:.|:.|::|.:|.||||||.||.:|::..||.:||.:.|.|||||...||.:
  Rat   602 NGKVQHCRIHSRQDAGTPKFFLTDNLVFDSLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESK 666

  Fly   700 EWFHPNTTKEQAEQGLYRL-EIGSFLVRPSVQSINAFVISFTINRKIKHCRIMQEGRLYGIDTMN 763
            ||:|.:.|:.|||..|.|: ..|:||||...:. |::.|||....||||||:.|||:...:....
  Rat   667 EWYHASLTRAQAEHMLMRVPRDGAFLVRKRNEP-NSYAISFRAEGKIKHCRVQQEGQTVMLGNSE 730

  Fly   764 FESLVSLINYYTRNPLYRNVKLSHPVSQELLRQALAEAAQGDHSGGHDDNGASNYMGSNLEENVT 828
            |:|||.||:||.::||||.:||.:|:::|.|.:  ...|:.|:...::......|:.:|......
  Rat   731 FDSLVDLISYYEKHPLYRKMKLRYPINEEALEK--IGTAEPDYGALYEGRNPGFYVEANPMPTFK 793

  Fly   829 C--KALYSYKANKPDELSFPKHAIITNVQRDNSMWWIGDYGGMIKKHLPANYVK------VIDST 885
            |  |||:.|||.:.|||:|.|.|||.||::.:..||.|||||..:...|:|||:      :::..
  Rat   794 CAVKALFDYKAQREDELTFTKSAIIQNVEKQDGGWWRGDYGGKKQLWFPSNYVEEMINPAILEPE 858

  Fly   886 TE--DYNS------------------LNEEGTDGRTDSIEIFGAVASLFESNDPGIIFKLQIQTP 930
            .|  |.||                  :..||.:.|                     :|...|..|
  Rat   859 REHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNR---------------------LFVFSISMP 902

  Fly   931 TM-QNPFVIGFDNQETAYEWIKAIQEAALIASQLASERRKKERTARVAKEMSDLIIYFRSVPFRE 994
            :: |....:..|:||...:|:|.|:|.|..|....:|.:..||..::|.|:|:|::|.|.|||.|
  Rat   903 SVAQWSLDVAADSQEELQDWVKKIREVAQTADARLTEGKMMERRKKIALELSELVVYCRPVPFDE 967

  Fly   995 HS-----WIFQEMSSFPETKAEKQFFQQNTQLFLSYHRNQISRVYPKGQRLDSSNFNPMPFWNVG 1054
            ..     ..:::|||||||||||...:...:.||.|:|.|:||:|||||||||||::|:|.|..|
  Rat   968 EKIGTERACYRDMSSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWICG 1032

  Fly  1055 SQMIALNYQTGDKAMQLNQAKFRNNGQCGYILKPSFMKSDSFNP-NNPLCDGLSEVKVSIRLIAA 1118
            ||::|||:||.||.||:|||.|...|.|||:|:||.|:.::|:| :.....||....:.|.::.|
  Rat  1033 SQLVALNFQTPDKPMQMNQALFMAGGHCGYVLQPSTMRDEAFDPFDKSSLRGLEPCVICIEVLGA 1097

  Fly  1119 RHLFRGGKS-NNPQIVVELIGASFDTGVKYRTK-VNENGFNPVW-NESCEFNVRNPQFAILRFEV 1180
            |||.:.|:. ..|.:.:|:.||.:|: .|.:|: |.:||.|||| .:...|.:.||:||.|||.|
  Rat  1098 RHLPKNGRGIVCPFVEIEVAGAEYDS-TKQKTEFVVDNGLNPVWPAKPFHFQISNPEFAFLRFVV 1161

  Fly  1181 QDEDMFAETHFIAQACYPLTCIRQGYRSVILRNKFSEELELSSLLINIKI 1230
            .:||||::.:|:|||.:|:..::.|||:|.|:|.:||:|||:||||.|.|
  Rat  1162 YEEDMFSDQNFLAQATFPVKGLKTGYRAVPLKNNYSEDLELASLLIKIDI 1211

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sl	NP_476726.2	PH_PLC_gamma	22..147	CDD:270168	53/126 (42%)
		PI-PLCc_gamma	323..>470	CDD:176534	104/147 (71%)
		SH2_N-SH2_PLC_gamma_like	584..682	CDD:199829	52/103 (50%)
		SH2_C-SH2_PLC_gamma_like	696..796	CDD:198186	46/100 (46%)
		SH3_PLCgamma	828..880	CDD:212759	27/53 (51%)
		PLCYc	981..1091	CDD:128454	63/114 (55%)
		C2_PLC_like	1109..1215	CDD:175974	44/108 (41%)
Plcg1	NP_037319.2	PH_PLC_gamma	29..151	CDD:270168	53/126 (42%)
		EFh_PI-PLC	156..306	CDD:333715	42/169 (25%)
		EF-hand motif	156..185	CDD:320029	8/38 (21%)
		EF-hand motif	193..221	CDD:320029	8/27 (30%)
		EF-hand motif	226..262	CDD:320029	8/35 (23%)
		EF-hand motif	272..306	CDD:320029	13/33 (39%)
		PI-PLCc_gamma	319..>466	CDD:176534	104/146 (71%)
		PH-like	489..>523	CDD:418428	15/34 (44%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	522..546		8/40 (20%)
		SH2_N-SH2_PLC_gamma_like	545..649	CDD:199829	52/103 (50%)
		SH2_C-SH2_PLC_gamma_like	663..765	CDD:198186	46/104 (44%)
		SH3_PLCgamma1	791..850	CDD:212903	28/58 (48%)
		PHsplit_PLC_gamma	<864..933	CDD:270054	17/89 (19%)
		PLCYc	954..1069	CDD:128454	63/114 (55%)
		C2_PLC_like	1087..1211	CDD:175974	54/124 (44%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1271..1290
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166345384
Domainoid	1	1.000	225	1.000	Domainoid score	I2449
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H1997
Inparanoid	1	1.050	922	1.000	Inparanoid score	I338
OMA	1	1.010	-	-		QHG45872
OrthoDB	1	1.010	-	-		D368239at2759
OrthoFinder	1	1.000	-	-		FOG0003159
OrthoInspector	1	1.000	-	-		otm45622
orthoMCL	1	0.900	-	-		OOG6_104615
Panther	1	1.100	-	-	LDO	PTHR10336
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X101
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	13	12.910