DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sl and Plcg1

DIOPT Version :9

Sequence 1:	NP_476726.2	Gene:	sl / 32601	FlyBaseID:	FBgn0003416	Length:	1236	Species:	Drosophila melanogaster
Sequence 2:	NP_067255.2	Gene:	Plcg1 / 18803	MGIID:	97615	Length:	1302	Species:	Mus musculus

Alignment Length:	1284	Identity:	530/1284 - (41%)
Similarity:	762/1284 - (59%)	Gaps:	150/1284 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 APLLGEMEQTIGMLERGTIVTKLYGKQ-RRPDRRHLMLIRETRQLLWATVATQTPRTDYEGAIQL 72
            ||...|:......||.||::|..|.|: :||:|:...:..||||:.|:..|.:     .||:|.:
Mouse    16 APSEAEVLHLCRSLEVGTVMTLFYSKKSQRPERKTFQVKLETRQITWSRGADK-----IEGSIDI 75

  Fly    73 REIREIRVGKHSKEFRLFADD-CQRFESSKCFVILHGNHFKLKSFSVVALSEIEADNWVRGLRYM 136
            |||:|||.||.|::|..:.:| ..|.:.|.|||||:|..|:||:.|:.|.||.|.:.|::||.::
Mouse    76 REIKEIRPGKTSRDFDRYQEDPAFRPDQSHCFVILYGMEFRLKTLSLQATSEDEVNMWIKGLTWL 140

  Fly   137 VKDTLGAPYPLQIDRWLRREYYQIENVNTHSAKATEQSPAQVTIKDFKLFLAGVSCKMTTGKFM- 200
            ::|||.|..||||:||||:::|.::          .....:::.||.|..|:.|:.::...:|:
Mouse   141 MEDTLQAATPLQIERWLRKQFYSVD----------RNREDRISAKDLKNMLSQVNYRVPNMRFLR 195

  Fly   201 EHFTEDVRRKHDLKFDDFSRLYQKLLLPNGFASVLSGSGVANFPYSEDQQV---VRP-------A 255
            |..|:..:|..|:.:..|::||:.|:        .|.....:.|:.|...:   .||       :
Mouse   196 ERLTDLEQRSGDITYGQFAQLYRSLM--------YSAQKTMDLPFLETNALRTGERPEHCQVSLS 252

  Fly   256 ELKQFLETEQRDVSASEISSAAIASFIRDFVQDVERDVQEPYLTFPEFVDFLFSKQNDLWNSKYD 320
            |.:|||...|.::.|  :....:..|:..|::|..|:::|||....|.|.|||||:|.:|||:.|
Mouse   253 EFQQFLLEYQGELWA--VDRLQVQEFMLSFLRDPLREIEEPYFFLDELVTFLFSKENSVWNSQLD 315

  Fly   321 QVFMD-MNLPLSSYWIASSHNTYLTGDQFSSESSCEAYARALRMGCRCIELDCWNGPDNLPYIFH 384
            .|..| ||.|||.|||:|||||||||||||||||.|||||.|||||||||||||:|||.:|.|:|
Mouse   316 AVCPDTMNNPLSHYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDGMPVIYH 380

  Fly   385 GHTITSKIKFMDVIKTIKDHAFTSSEYPVILSIEQNCSLEQQRNMAQALIEVFGDMLLTQPCDRN 449
            |||:|:||||.||:.|||:|||.:||||||||||.:||:.|||||||...:|.||.|||:|.|..
Mouse   381 GHTLTTKIKFSDVLHTIKEHAFVASEYPVILSIEDHCSIAQQRNMAQHFRKVLGDTLLTKPVDIA 445

  Fly   450 EQHLPSPYQLRRKIILKHKKLPQ---FDDIANGISSTGSLGHRSSLGGAGGGAHGENDGENVRKV 511
            ...||||.||||||::|||||.:   ::::...:                  .:.|||   :...
Mouse   446 ADGLPSPNQLRRKILIKHKKLAEGSAYEEVPTSV------------------MYSEND---ISNS 489

  Fly   512 FKEGLLYFKDPVDKSWNLYQFVLTHQELIYSSEINESRNGNSEDDDFGLSSSCSLNSNMQQKQKD 576
            .|.|:||.:|||:..|..:.||||..::.||.| ..|..||.::::                .|:
Mouse   490 IKNGILYLEDPVNHEWYPHYFVLTSSKIYYSEE-TSSDQGNEDEEE----------------PKE 537

  Fly   577 TSANDELHFGENWFHGKL---EGGRKEADDLLKKY---KHFGDGTFLVRESATFVGDYSLSFWRR 635
            .|::.|||..|.||||||   ..||..|:.||.:|   ....||:||||||.||||||:|||||.
Mouse   538 ASSSTELHSSEKWFHGKLGAGRDGRHIAERLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWRN 602

  Fly   636 NRPNHCRIKLKHENGSIKYYLVENFVFDSLYSLIVYYRKNMLRSSEFSIILKEPVPQPKKHEDQE 700
            .:..||||..:.:.|:.|::|.:|.||||||.||.:|::..||.:||.:.|.|||||...||.:|
Mouse   603 GKVQHCRIHSRQDAGTPKFFLTDNLVFDSLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESKE 667

  Fly   701 WFHPNTTKEQAEQGLYRL-EIGSFLVRPSVQSINAFVISFTINRKIKHCRIMQEGRLYGIDTMNF 764
            |:|.:.|:.|||..|.|: ..|:||||...:. |::.|||....||||||:.|||:...:....|
Mouse   668 WYHASLTRAQAEHMLMRVPRDGAFLVRKRNEP-NSYAISFRAEGKIKHCRVQQEGQTVMLGNSEF 731

  Fly   765 ESLVSLINYYTRNPLYRNVKLSHPVSQELLRQALAEAAQGDHSGGHDDNGASNYMGSNLEENVTC 829
            :|||.||:||.::||||.:||.:|:::|.|.:  ...|:.|:...::......|:.:|......|
Mouse   732 DSLVDLISYYEKHPLYRKMKLRYPINEEALEK--IGTAEPDYGALYEGRNPGFYVEANPMPTFKC 794

  Fly   830 --KALYSYKANKPDELSFPKHAIITNVQRDNSMWWIGDYGGMIKKHLPANYVK------VIDSTT 886
              |||:.|||.:.|||:|.|.|||.||::.:..||.|||||..:...|:|||:      |::...
Mouse   795 AVKALFDYKAQREDELTFTKSAIIQNVEKQDGGWWRGDYGGKKQLWFPSNYVEEMINPAVLEPER 859

  Fly   887 E--DYNS------------------LNEEGTDGRTDSIEIFGAVASLFESNDPGIIFKLQIQTPT 931
            |  |.||                  :..||.:.|                     :|...|..|:
Mouse   860 EHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNR---------------------LFVFSISMPS 903

  Fly   932 M-QNPFVIGFDNQETAYEWIKAIQEAALIASQLASERRKKERTARVAKEMSDLIIYFRSVPFREH 995
            : |....:..|:||...:|:|.|:|.|..|....:|.:..||..::|.|:|:|::|.|.|||.|.
Mouse   904 VAQWSLDVAADSQEELQDWVKKIREVAQTADARLTEGKMMERRKKIALELSELVVYCRPVPFDEE 968

  Fly   996 S-----WIFQEMSSFPETKAEKQFFQQNTQLFLSYHRNQISRVYPKGQRLDSSNFNPMPFWNVGS 1055
            .     ..:::|||||||||||...:...:.||.|:|.|:||:|||||||||||::|:|.|..||
Mouse   969 KIGTERACYRDMSSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWICGS 1033

  Fly  1056 QMIALNYQTGDKAMQLNQAKFRNNGQCGYILKPSFMKSDSFNP-NNPLCDGLSEVKVSIRLIAAR 1119
            |::|||:||.||.||:|||.|...|.|||:|:||.|:.::|:| :.....||....:.|.::.||
Mouse  1034 QLVALNFQTPDKPMQMNQALFMAGGHCGYVLQPSTMRDEAFDPFDKSSLRGLEPCVICIEVLGAR 1098

  Fly  1120 HLFRGGKS-NNPQIVVELIGASFDTGVKYRTK-VNENGFNPVW-NESCEFNVRNPQFAILRFEVQ 1181
            ||.:.|:. ..|.:.:|:.||.:|: .|.:|: |.:||.|||| .:...|.:.||:||.|||.|.
Mouse  1099 HLPKNGRGIVCPFVEIEVAGAEYDS-TKQKTEFVVDNGLNPVWPAKPFHFQISNPEFAFLRFVVY 1162

  Fly  1182 DEDMFAETHFIAQACYPLTCIRQGYRSVILRNKFSEELELSSLLINIKI 1230
            :||||::.:|:|||.:|:..::.|||:|.|:|.:||:|||:||||.|.|
Mouse  1163 EEDMFSDQNFLAQATFPVKGLKTGYRAVPLKNNYSEDLELASLLIKIDI 1211

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sl	NP_476726.2	PH_PLC_gamma	22..147	CDD:270168	53/126 (42%)
		PI-PLCc_gamma	323..>470	CDD:176534	106/147 (72%)
		SH2_N-SH2_PLC_gamma_like	584..682	CDD:199829	52/103 (50%)
		SH2_C-SH2_PLC_gamma_like	696..796	CDD:198186	46/100 (46%)
		SH3_PLCgamma	828..880	CDD:212759	27/53 (51%)
		PLCYc	981..1091	CDD:128454	63/114 (55%)
		C2_PLC_like	1109..1215	CDD:175974	44/108 (41%)
Plcg1	NP_067255.2	PH_PLC_gamma	29..151	CDD:270168	53/126 (42%)
		PH	<71..142	CDD:278594	32/70 (46%)
		PI-PLCc_gamma	319..>466	CDD:176534	106/146 (73%)
		PH-like	489..>523	CDD:302622	15/34 (44%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	522..545		7/39 (18%)
		SH2_N-SH2_PLC_gamma_like	545..649	CDD:199829	52/103 (50%)
		SH2_C-SH2_PLC_gamma_like	663..765	CDD:198186	46/104 (44%)
		SH3_PLCgamma1	791..850	CDD:212903	28/58 (48%)
		PHsplit_PLC_gamma	<864..933	CDD:270054	17/89 (19%)
		PH	<872..929	CDD:278594	13/77 (17%)
		PLCYc	954..1069	CDD:128454	63/114 (55%)
		C2_PLC_like	1087..1211	CDD:175974	54/124 (44%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167841989
Domainoid	1	1.000	226	1.000	Domainoid score	I2507
eggNOG	1	0.900	-	-		E1_KOG0169
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H1997
Inparanoid	1	1.050	923	1.000	Inparanoid score	I354
Isobase	1	0.950	-	0	Normalized mean entropy	S3205
OMA	1	1.010	-	-		QHG45872
OrthoDB	1	1.010	-	-		D368239at2759
OrthoFinder	1	1.000	-	-		FOG0003159
OrthoInspector	1	1.000	-	-		otm43555
orthoMCL	1	0.900	-	-		OOG6_104615
Panther	1	1.100	-	-	LDO	PTHR10336
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R928
SonicParanoid	1	1.000	-	-		X101
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	17	16.750