DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment CalpC and CAPN2

DIOPT Version :9

Sequence 1:	NP_573118.2	Gene:	CalpC / 32597	FlyBaseID:	FBgn0260450	Length:	681	Species:	Drosophila melanogaster
Sequence 2:	NP_001739.3	Gene:	CAPN2 / 824	HGNCID:	1479	Length:	700	Species:	Homo sapiens

Alignment Length:	708	Identity:	197/708 - (27%)
Similarity:	341/708 - (48%)	Gaps:	81/708 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly     5 YERILSDCRSKNVLWEDPDFPAVQSSVFYYQTPPFT-----FQWKRIMDLADSGSGAVAANSSAA 64
            ||.:.::|.....|::||.|||:.|::.:.:..|::     .:|||..::.            |.
Human    32 YEALRNECLEAGTLFQDPSFPAIPSALGFKELGPYSSKTRGIEWKRPTEIC------------AD 84

  Fly    65 PVF-LNESAEFDVVPGKMGDRWLVSCLGLLSSLRNLFYRVVPADQTLASAH-GVFRFRLWWCGEW 127
            |.| :..:...|:..|.:||.||::.:..|:....:..||||.:|:....: |:|.|:.|..|||
Human    85 PQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPLNQSFQENYAGIFHFQFWQYGEW 149

  Fly   128 VEVLVDDRLPTINGRLAFMQPQASNCFWAALLEKAIAKLHGSYEALKYGTRSDGLTDLLGGVV-- 190
            |||:|||||||.:|.|.|:.....:.||:||||||.||::|.||||..|..::|..|..||:.
Human   150 VEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEW 214

  Fly   191 ----RQMPILSDNIRPQTLK-ELLTTTCIVTCLADKSATVAKKNLAERMPNGILVNVNYRLSSLD 250
                :..|.|...|:....| .||..:..:|..||..|...:|         ::....|.::..:
Human   215 YELKKPPPNLFKIIQKALQKGSLLGCSIDITSAADSEAITFQK---------LVKGHAYSVTGAE 270

  Fly   251 KVKTLMGDSVQLVCLKDTFSSKPFGEKTHFLGDWSPMSKTWERVSQVERARLIRQLGPGEFWLSF 315
            :|:: .|...:|:.:::     |:|| ..:.|.|:....:|..:...||.||.|:...||||:||
Human   271 EVES-NGSLQKLIRIRN-----PWGE-VEWTGRWNDNCPSWNTIDPEERERLTRRHEDGEFWMSF 328

  Fly   316 CDFVEIFSTMEVVYLDTETSNDEEMLKSRPLHWKMKMHQGQWKRGVTAGGCRNH-ESFHINPQLL 379
            .||:..:|.:|:..|..:|...:...|     ||:....|.|:||.|||||||: .:|.:|||.|
Human   329 SDFLRHYSRLEICNLTPDTLTSDTYKK-----WKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYL 388

  Fly   380 ISV----QDEQD-------LVIALNQHTAVEPKV------IGFTMYTWDGEY--MLSECLQKDFF 425
            |.:    :||:|       ||..:.:|...:.|:      |||.:|....|.  ..:..|.|:||
Human   389 IKLEEEDEDEEDGESGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELSGQTNIHLSKNFF 453

  Fly   426 KNHVSYLNSD-YGNTRHVSYHTHLEAGHYVLIPTTYEPAEEAHFTVRILGTGSFRLSCLETQTMI 489
            ..:.:...|| :.|.|.|.....|..|.|:|:|:|:||.::..|.:|:..........::.:...
Human   454 LTNRARERSDTFINLREVLNRFKLPPGEYILVPSTFEPNKDGDFCIRVFSEKKADYQAVDDEIEA 518

  Fly   490 LLDPFPALKSTDAERCGGPKVKSVCQYEPVYMQLADENKTINCFELHELLEACLP--NDYIKGCA 552
            .|:.|. :...|.:.          .:..::.|||.|:..|:.|||..:|...|.  .|......
Human   519 NLEEFD-ISEDDIDD----------GFRRLFAQLAGEDAEISAFELQTILRRVLAKRQDIKSDGF 572

  Fly   553 NIDICRQVIALQDRSGSGRITFQQFKTFMVNLKSWQGVFKMYTKEKAGILRAERLRDALCDIGFQ 617
            :|:.|:.::.:.|..|||::..::|......::.:|.:::....:::|.:.:..:|.||.:.||:
Human   573 SIETCKIMVDMLDSDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFK 637

  Fly   618 LSTDIMNCLIQRYIRKDGTLRLSDFVSAVIHLTTAFNQFHLKNYGQVNVIEVHLHDWI 675
            :...:...::.|:......:...:||..::.|.|.|..|...:......||:.|..|:
Human   638 MPCQLHQVIVARFADDQLIIDFDNFVRCLVRLETLFKIFKQLDPENTGTIELDLISWL 695

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CalpC	NP_573118.2	CysPc	7..329	CDD:238004	103/335 (31%)
		Calpain_III	346..483	CDD:238132	50/157 (32%)
		FRQ1	<567..648	CDD:227455	14/80 (18%)
CAPN2	NP_001739.3	Peptidase_C2	46..342	CDD:306994	101/323 (31%)
		Domain III	345..514		52/173 (30%)
		Calpain_III	363..507	CDD:307285	48/143 (34%)
		Linker	515..529		2/14 (14%)
		Domain IV	530..700		36/176 (20%)
		EFh_PEF_CAPN2	533..700	CDD:320074	36/163 (22%)
		EF-hand motif	533..561	CDD:320074	9/27 (33%)
		EF-hand motif	576..605	CDD:320074	6/28 (21%)
		EF-hand motif	606..636	CDD:320074	5/29 (17%)
		EF-hand motif	642..670	CDD:320074	3/27 (11%)
		EF-hand motif	672..700	CDD:320074	6/24 (25%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG0045
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0	Normalized mean entropy	S2747
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D704215at2759
OrthoFinder	1	1.000	-	-		FOG0000071
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR10183
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	6	5.870